##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552402_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	837195
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.227317411116886	31.0	31.0	34.0	30.0	34.0
2	31.42256941333859	31.0	31.0	34.0	28.0	34.0
3	31.478069028123674	31.0	31.0	34.0	28.0	34.0
4	35.211540919379594	37.0	35.0	37.0	32.0	37.0
5	35.05442698534989	37.0	35.0	37.0	32.0	37.0
6	35.158162674167905	37.0	35.0	37.0	32.0	37.0
7	35.60647280502153	37.0	35.0	37.0	35.0	37.0
8	35.63960725995736	37.0	35.0	37.0	35.0	37.0
9	37.144157573803	39.0	37.0	39.0	34.0	39.0
10	36.54192511899856	38.0	35.0	39.0	32.0	39.0
11	36.321105596665056	38.0	35.0	39.0	32.0	39.0
12	36.33658586111957	38.0	35.0	39.0	32.0	39.0
13	36.09049026809763	38.0	35.0	39.0	31.0	39.0
14	37.2214155602936	39.0	36.0	40.0	32.0	41.0
15	37.33268115552529	39.0	36.0	40.0	32.0	41.0
16	37.33950752214239	39.0	36.0	40.0	32.0	41.0
17	37.36219877089567	39.0	36.0	40.0	32.0	41.0
18	37.312196083349754	39.0	36.0	40.0	32.0	41.0
19	37.23420588990618	39.0	36.0	40.0	32.0	41.0
20	36.85110278967266	39.0	35.0	40.0	31.0	41.0
21	36.94501758849491	39.0	35.0	40.0	31.0	41.0
22	37.06098937523516	39.0	35.0	40.0	31.0	41.0
23	36.88696898572017	39.0	35.0	40.0	31.0	41.0
24	36.93330586064179	39.0	35.0	40.0	31.0	41.0
25	36.94446574573427	39.0	35.0	40.0	31.0	41.0
26	36.907303555324624	39.0	35.0	40.0	31.0	41.0
27	36.710335107113636	39.0	35.0	40.0	31.0	41.0
28	36.710208493839545	39.0	35.0	40.0	31.0	41.0
29	36.75304558675099	39.0	35.0	40.0	31.0	41.0
30	36.61142983414855	38.0	35.0	40.0	30.0	41.0
31	36.52617132209342	38.0	35.0	40.0	30.0	41.0
32	36.27347272738132	38.0	35.0	40.0	30.0	41.0
33	36.16902274858307	38.0	35.0	40.0	30.0	41.0
34	36.13556817706747	38.0	35.0	40.0	30.0	41.0
35	35.96666487496939	38.0	35.0	40.0	29.0	41.0
36	36.008896374201946	38.0	35.0	40.0	29.0	41.0
37	36.018625290404266	38.0	35.0	40.0	30.0	41.0
38	35.9613375617389	38.0	35.0	40.0	29.0	41.0
39	35.94515734088235	38.0	35.0	40.0	29.0	41.0
40	35.85094273138277	38.0	35.0	40.0	29.0	41.0
41	35.77742700326686	38.0	35.0	40.0	29.0	41.0
42	35.75499495338601	38.0	35.0	40.0	29.0	41.0
43	35.63088527762349	38.0	34.0	40.0	28.0	41.0
44	35.53695495075819	38.0	34.0	40.0	28.0	41.0
45	35.49846212650577	38.0	34.0	40.0	28.0	41.0
46	35.37305765084598	38.0	34.0	40.0	27.0	41.0
47	35.28331511774437	38.0	34.0	40.0	27.0	41.0
48	35.22508495631245	38.0	34.0	40.0	27.0	41.0
49	35.14444543983182	38.0	34.0	40.0	27.0	41.0
50	35.0245904478646	37.0	34.0	40.0	27.0	41.0
51	33.73556101027837	36.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	17.0
9	31.0
10	54.0
11	37.0
12	16.0
13	14.0
14	20.0
15	36.0
16	76.0
17	184.0
18	356.0
19	586.0
20	976.0
21	1605.0
22	2299.0
23	3409.0
24	4968.0
25	6608.0
26	8274.0
27	10118.0
28	12555.0
29	15680.0
30	20217.0
31	25964.0
32	33521.0
33	44358.0
34	63461.0
35	75649.0
36	82445.0
37	110578.0
38	157957.0
39	155110.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.545762934561246	20.076326303907692	25.69114722376507	11.686763537765993
2	31.95802650517502	24.049474734082263	25.483071446914995	18.50942731382772
3	31.07925871511416	24.116125872705883	27.700953780182637	17.103661631997326
4	27.56585980566057	26.448437938592562	27.463971953965327	18.521730301781545
5	23.907452863430862	29.527290535657762	26.127485233428295	20.437771367483084
6	24.462520679172712	35.88793530778373	25.40184783712277	14.247696175920781
7	79.55972025633216	6.4793745782046	8.98058397386511	4.980321191598134
8	79.77950178871112	5.320863120300527	8.824586864470046	6.075048226518314
9	74.224643004318	7.325652924348569	11.4276841118258	7.022019959507642
10	36.039632343719205	25.060947568965414	20.810802740102368	18.088617347213017
11	27.578760026039333	26.6308327211701	25.05545303065594	20.734954222134625
12	24.108959083606567	23.942211790562535	31.062775100185743	20.88605402564516
13	23.493331899975516	24.72972246609213	32.79606304385478	18.98088259007758
14	19.964643840443387	28.434952430437356	30.07423599042039	21.52616773869887
15	18.424978648940808	26.97818309951684	34.52278143084944	20.07405682069291
16	20.906598821063195	26.16869427074935	31.485137871105294	21.439569037082162
17	20.830989196065435	25.807368653658948	29.021793011186166	24.33984913908946
18	21.5508931610915	25.543630814804196	31.127873434504505	21.777602589599795
19	21.249051893525404	27.97484457026141	29.0725577673063	21.703545768906885
20	22.67715406804866	27.297583000376257	29.06801880087674	20.957244130698342
21	21.484958701377817	28.5921440046823	29.868071357330134	20.05482593660975
22	19.740442788119854	26.21778677607965	27.783252408339752	26.25851802746075
23	19.608215529237512	28.153058725864344	28.63132245175855	23.60740329313959
24	20.63653031850405	25.86792802154815	29.757463912230726	23.738077747717078
25	19.779262895741134	27.438052066722808	28.20310680307455	24.579578234461504
26	18.756681537754048	27.791852555258927	30.28541737588017	23.16604853110685
27	21.243915694670896	27.017480993078074	30.272636601986395	21.465966710264635
28	18.008707648755667	26.558687044237004	31.66287424076828	23.769731066239046
29	20.003822287519636	25.691863902675006	30.144351077108677	24.159962732696684
30	22.10046643852388	26.56489826145641	28.831753653569358	22.502881646450348
31	21.172367250162747	27.723409719360482	27.351333918621112	23.75288911185566
32	20.635455300139157	26.725912123220997	28.43017457103781	24.20845800560204
33	22.261480300288465	26.27798780451388	26.590101469788998	24.870430425408657
34	19.07643977806843	24.153154283052338	30.930786734273376	25.839619204605857
35	20.649669431852793	23.37066035989226	30.059185733311832	25.920484474943112
36	23.1112225944971	26.099176416486003	29.09095252599454	21.698648463022355
37	20.86515089077216	24.874491605898267	29.986681716923773	24.273675786405796
38	19.527350258900256	27.5383871141132	28.72305735222977	24.21120527475678
39	21.919027227826255	25.165821582785377	27.028470069697025	25.886681119691353
40	21.326931001737947	23.519849019642976	30.82424046966358	24.328979508955502
41	20.521025567520113	23.76125036580486	29.972348138725145	25.745375927949883
42	23.50264872580462	23.289675643070012	30.542705104545536	22.66497052657983
43	23.266383578497244	22.55830481548504	30.329134789385986	23.84617681663173
44	20.717156695871335	23.866243826109805	29.10349440691834	26.313105071100516
45	21.62662223257425	22.543015665406507	28.41703545768907	27.413326644330173
46	22.269602661267683	24.23593069714941	29.175520637366443	24.31894600421646
47	19.864786578992945	24.13010111144954	31.480360011705756	24.524752297851755
48	20.314024809034933	23.380096632206357	31.492543553174592	24.813335005584122
49	22.50527057615012	22.120175108546995	31.277539880195178	24.09701443510771
50	20.103560102485083	21.557104378310907	30.86509116752967	27.474244351674344
51	19.67080548737152	21.679298132454207	28.657600678456035	29.992295701718234
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2633.0
1	2418.0
2	2203.0
3	2701.5
4	3200.0
5	2416.0
6	1632.0
7	1613.0
8	1594.0
9	1639.5
10	1685.0
11	1763.5
12	1842.0
13	2009.5
14	2177.0
15	2235.0
16	2293.0
17	2358.5
18	2424.0
19	2558.0
20	2692.0
21	2720.0
22	2748.0
23	3066.0
24	3384.0
25	3730.5
26	5136.0
27	6195.0
28	7418.5
29	8642.0
30	9783.5
31	10925.0
32	12403.5
33	13882.0
34	16478.0
35	19074.0
36	21704.0
37	24334.0
38	26608.0
39	28882.0
40	32769.5
41	36657.0
42	42498.5
43	48340.0
44	55103.5
45	61867.0
46	73097.0
47	84327.0
48	94576.5
49	104826.0
50	100370.0
51	95914.0
52	81890.5
53	67867.0
54	57688.0
55	47509.0
56	41241.0
57	34973.0
58	31613.0
59	28253.0
60	26361.0
61	24469.0
62	20927.0
63	17385.0
64	15541.0
65	13697.0
66	11118.5
67	8540.0
68	7395.0
69	6250.0
70	5089.5
71	3929.0
72	3226.0
73	2523.0
74	2031.5
75	1219.0
76	898.0
77	640.5
78	383.0
79	322.0
80	261.0
81	209.5
82	158.0
83	104.0
84	50.0
85	38.5
86	27.0
87	25.5
88	24.0
89	12.0
90	0.0
91	3.5
92	7.0
93	5.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	837195.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.01575734504636
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.39236015588116	24.99857559508491
2	11.995286417049977	8.40048076927505
3	4.605649792758103	4.838109466784424
4	2.3432276687682263	3.281995658151475
5	1.4667852606414318	2.5680298381955473
6	0.9928850655676159	2.085997351646165
7	0.7345291642574989	1.8004066484950196
8	0.5661789402812417	1.5860147509410765
9	0.4623738182610876	1.4571332480639527
>10	5.165874015360763	37.969931986382164
>50	0.21711399259833733	4.768938848302392
>100	0.05116697526365092	3.279204443373877
>500	0.0034572280583543623	0.7447454864011618
>1k	0.0031115052525189264	2.2204359089028136
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGC	3508	0.41901826933987907	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGCT	3099	0.37016465697955675	TruSeq Adapter, Index 13 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2359	0.2817742580880201	No Hit
CCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGC	2307	0.27556304086861483	TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCC	1801	0.21512311946440196	No Hit
TCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGC	1616	0.1930255197415178	TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1484	0.1772585837230275	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1129	0.13485508155208764	No Hit
ACTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGC	1039	0.12410489790311695	TruSeq Adapter, Index 13 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4226016638895359	0.0
2	0.0	0.0	0.0	1.5602099869206099	0.0
3	0.0	0.0	0.0	2.127341897646307	0.0
4	0.0	0.0	0.0	2.811770256630773	0.0
5	0.0	0.0	0.0	4.542072038175097	0.0
6	0.0	0.0	0.0	5.631065641815826	0.0
7	0.0	0.0	0.0	6.760073817927723	0.0
8	0.0	0.0	0.0	8.651628354206606	0.0
9	0.0	0.0	0.0	9.314914685348096	0.0
10	0.0	0.0	0.0	11.198466307132747	0.0
11	0.0	0.0	0.0	13.873231445481638	0.0
12	0.0	0.0	0.0	15.729310375718919	0.0
13	0.0	0.0	0.0	16.43571688794128	0.0
14	0.0	0.0	0.0	16.6965880111563	0.0
15	0.0	0.0	0.0	17.233380514694904	0.0
16	0.0	0.0	0.0	18.439073334169457	0.0
17	0.0	0.0	0.0	19.868489420027593	0.0
18	0.0	0.0	0.0	21.513028625350127	0.0
19	0.0	0.0	0.0	22.403382724454875	0.0
20	0.0	0.0	0.0	23.276297636751295	0.0
21	0.0	0.0	0.0	24.3853582498701	0.0
22	0.0	0.0	0.0	25.475904657815683	0.0
23	0.0	0.0	0.0	26.57146781813078	0.0
24	0.0	0.0	0.0	27.379045503138457	0.0
25	0.0	0.0	0.0	28.146369722704986	0.0
26	0.0	0.0	0.0	28.81742007537073	0.0
27	0.0	0.0	0.0	29.496115003075747	0.0
28	0.0	0.0	0.0	30.17146542920108	0.0
29	0.0	0.0	0.0	30.86485227455969	0.0
30	0.0	0.0	0.0	31.661679775918394	0.0
31	0.0	0.0	0.0	32.37561141669504	0.0
32	0.0	0.0	0.0	33.07162608472339	0.0
33	1.19446484988563E-4	0.0	0.0	33.78113820555546	0.0
34	1.19446484988563E-4	0.0	0.0	34.463774867265094	0.0
35	1.19446484988563E-4	0.0	0.0	35.20219303746439	0.0
36	1.19446484988563E-4	0.0	0.0	35.85437084550195	0.0
37	1.19446484988563E-4	0.0	0.0	36.514432121548744	0.0
38	1.19446484988563E-4	0.0	0.0	37.15335136975256	0.0
39	1.19446484988563E-4	0.0	0.0	37.80911257233978	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGTA	45	3.8380676E-10	45.0	41
TCGTTTA	25	3.889895E-5	45.0	38
GTCGACG	25	3.889895E-5	45.0	1
CGACGGT	25	3.889895E-5	45.0	28
TCGGTTC	100	0.0	45.0	13
CTAACGG	60	0.0	44.999996	2
GCGTTAG	80	0.0	42.1875	1
TATGGGT	240	0.0	41.249996	4
GCGTAAG	105	0.0	40.714287	1
CGTTAGG	150	0.0	40.500004	2
CGTTTTT	1475	0.0	39.661015	1
ACGCATT	40	3.4567165E-7	39.375	17
CCGATAG	40	3.4567165E-7	39.375	11
CATAGCG	40	3.4567165E-7	39.375	1
ACTAACG	40	3.4567165E-7	39.375	1
TATAGCG	40	3.4567165E-7	39.375	1
AATAGCG	40	3.4567165E-7	39.375	1
CTCGGTT	115	0.0	39.130432	12
GCAACGA	35	6.246255E-6	38.57143	11
CGCGCAA	35	6.246255E-6	38.57143	18
>>END_MODULE