FastQCFastQC Report
Sat 18 Jun 2016
SRR3552401_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552401_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016528
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA282902.7830025341161284No Hit
GCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC56010.5509931846442007Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGCT51290.5045606220389404Illumina Single End Adapter 1 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC37710.37096863047550094Illumina Single End Adapter 1 (95% over 21bp)
GAAACTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTC37580.36968976752238997No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37440.36831253049596274No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCC27600.27151244235279304No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22540.221735161254781No Hit
TCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC16160.15897250247902667Illumina Single End Adapter 1 (95% over 21bp)
GAAAACTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTT11680.1149009176333559No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTC11430.11244156580045014No Hit
CGCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTG11270.1108675806273905No Hit
GAAAAACTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCT11270.1108675806273905No Hit
ACTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC11210.11027733618749311Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCT10520.10348952512867328No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGC207.032903E-445.026
CTATGCG406.8139343E-945.01
GTCGCAA207.032903E-445.09
GCGAACT207.032903E-445.015
TAACGCG253.890487E-545.01
CTACGGT253.890487E-545.03
ACTCGGT207.032903E-445.011
ATGCGCA351.211738E-745.01
CAATGCG207.032903E-445.01
CGCGTAT207.032903E-445.024
CGATCTA207.032903E-445.010
TATCGAA207.032903E-445.031
TTAGCCG302.165254E-644.9999961
CCCTCGT750.042.014
GCGTAAG700.041.7857131
TTTCGCG556.184564E-1140.9090921
ACGCATT451.9279469E-840.017
TATACGG451.9279469E-840.02
CGTATGG1250.039.6000022
CTACGAA1250.039.60000211
Produced by FastQC (version 0.11.5)