##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552401_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1016528
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21751688099098	31.0	31.0	34.0	28.0	34.0
2	31.397244345458265	31.0	31.0	34.0	28.0	34.0
3	31.47876792375616	31.0	31.0	34.0	28.0	34.0
4	35.21386130042655	37.0	35.0	37.0	32.0	37.0
5	35.03827735192734	37.0	35.0	37.0	32.0	37.0
6	35.1340238537453	37.0	35.0	37.0	32.0	37.0
7	35.470289062377034	37.0	35.0	37.0	33.0	37.0
8	35.45978172760613	37.0	35.0	37.0	33.0	37.0
9	36.87776627894165	39.0	37.0	39.0	33.0	39.0
10	36.46320022665387	38.0	35.0	39.0	32.0	39.0
11	36.21499063478822	38.0	35.0	39.0	32.0	39.0
12	36.19422583539263	38.0	35.0	39.0	32.0	39.0
13	35.92214380715534	38.0	35.0	39.0	30.0	39.0
14	37.0519572505627	39.0	36.0	40.0	31.0	41.0
15	37.174590370358715	39.0	36.0	40.0	32.0	41.0
16	37.166595509420304	39.0	36.0	40.0	32.0	41.0
17	37.19470590087042	39.0	36.0	40.0	32.0	41.0
18	37.09089272504053	39.0	36.0	40.0	32.0	41.0
19	36.93526395731352	39.0	35.0	40.0	31.0	41.0
20	36.54160829804983	38.0	35.0	40.0	30.0	41.0
21	36.608502667904865	38.0	35.0	40.0	31.0	41.0
22	36.69681799222451	38.0	35.0	40.0	31.0	41.0
23	36.49770493286953	38.0	35.0	40.0	30.0	41.0
24	36.526472463129394	38.0	35.0	40.0	31.0	41.0
25	36.52996080776919	38.0	35.0	40.0	31.0	41.0
26	36.49457663733808	38.0	35.0	40.0	31.0	41.0
27	36.274218713109725	38.0	35.0	40.0	30.0	41.0
28	36.25245443312924	38.0	35.0	40.0	30.0	41.0
29	36.31482162813026	38.0	35.0	40.0	30.0	41.0
30	36.15630164638849	38.0	35.0	40.0	30.0	41.0
31	36.05051016794422	38.0	35.0	40.0	30.0	41.0
32	35.823824823320166	38.0	34.0	40.0	29.0	41.0
33	35.742585546094155	38.0	34.0	40.0	29.0	41.0
34	35.667124761934744	38.0	34.0	40.0	28.0	41.0
35	35.5442801378811	38.0	34.0	40.0	27.0	41.0
36	35.582437473439	38.0	34.0	40.0	28.0	41.0
37	35.576076605858376	38.0	34.0	40.0	28.0	41.0
38	35.53505658476697	38.0	34.0	40.0	28.0	41.0
39	35.4892850959344	38.0	34.0	40.0	28.0	41.0
40	35.34422268742228	38.0	34.0	40.0	27.0	41.0
41	35.25575685077047	38.0	34.0	40.0	27.0	41.0
42	35.277616553602066	38.0	34.0	40.0	27.0	41.0
43	35.18721963389105	38.0	34.0	40.0	27.0	41.0
44	35.09276576739647	37.0	34.0	40.0	26.0	41.0
45	35.03407677899674	37.0	34.0	40.0	26.0	41.0
46	34.903396660003466	37.0	33.0	40.0	26.0	41.0
47	34.770575921174824	37.0	33.0	40.0	26.0	41.0
48	34.70905867816725	37.0	33.0	40.0	25.0	41.0
49	34.609137180677756	37.0	33.0	40.0	25.0	41.0
50	34.45930264586907	37.0	33.0	40.0	24.0	41.0
51	33.23305604961201	35.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	19.0
9	55.0
10	80.0
11	68.0
12	35.0
13	25.0
14	17.0
15	61.0
16	118.0
17	288.0
18	561.0
19	1058.0
20	1711.0
21	2669.0
22	3813.0
23	5329.0
24	7328.0
25	9487.0
26	11658.0
27	14301.0
28	17731.0
29	21978.0
30	27567.0
31	34517.0
32	43629.0
33	57471.0
34	82983.0
35	102346.0
36	97214.0
37	124335.0
38	174702.0
39	173356.0
40	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.32929737301875	19.106212519478067	23.490941715329043	12.073548392174144
2	29.783045818708388	28.48736089906033	23.950250263642516	17.779343018588765
3	29.647978216045207	27.23771504572427	26.677376324115027	16.4369304141155
4	26.825822800749215	29.853383281129492	25.927274015078776	17.393519903042513
5	23.063014496403444	33.33887507279682	24.078136558953613	19.519973871846126
6	23.533636063148286	37.80948483465286	24.053543040624557	14.603336061574302
7	75.22576849826075	10.462869689767523	9.098421292871421	5.21294051910031
8	75.82771945288276	9.362063809358915	8.94426911998489	5.865947617773441
9	70.59667810429225	11.591515432924622	11.1088922292352	6.70291423354792
10	36.967402767065934	28.132230494388743	19.201143500228227	15.699223238317096
11	28.002966962051218	29.37567878110588	22.834393150016528	19.78696110682637
12	25.168514787590702	26.27768246423119	29.367808855240586	19.185993892937528
13	23.244416287598572	28.218209434467127	29.984614294933344	18.55275998300096
14	18.58719090866164	32.114609730376344	28.36272094816867	20.93547841279335
15	17.99183101695182	30.20851368580108	32.09818126013253	19.70147403711457
16	19.90491162073253	29.416799143752066	29.719988037712685	20.95830119780272
17	19.435568916940802	29.403912140147643	27.02739127697417	24.133127665937387
18	20.230136307115988	30.110041238411533	28.294547715360522	21.365274739111957
19	21.327794217178475	31.294956951505515	26.155305117025797	21.221943714290212
20	22.570455511309085	30.791478444273057	26.731875560732217	19.90619048368564
21	21.744113295452756	31.313844773582233	27.350648481891298	19.59139344907371
22	19.51761289408654	30.43133096186234	24.97806258165048	25.07299356240064
23	19.678749626178522	31.550631168054398	26.120579069145165	22.650040136621914
24	21.29739662852376	29.322458407441804	27.16442636110368	22.21571860293076
25	19.671371570679806	30.659853934175942	25.460292289046638	24.208482206097617
26	18.6111941825508	30.75675239639243	25.916354492940673	24.715698928116097
27	19.84864165079565	30.676675900713015	26.651799065052806	22.822883383438526
28	17.454806950718524	30.063608675806275	28.179941919947115	24.30164245352809
29	19.464884390789038	28.692962712291248	26.000759447846	25.841393449073706
30	20.504895093888216	29.330721829600364	26.34388821557301	23.82049486093841
31	20.540113012135425	31.64890686729731	24.378964475154643	23.432015645412623
32	21.07241512284954	30.552724568334565	23.52901248170242	24.845847827113467
33	20.400913698392962	30.025144413139625	25.31804337903137	24.25589850943604
34	18.311054884862983	29.052618324335384	26.99463271055987	25.641694080241763
35	19.968264523948186	27.749752097335247	24.29318228322289	27.98880109549368
36	20.19668912218847	30.98763634646562	25.713900650055876	23.10177388129004
37	19.69262037051611	31.16520154880141	26.967284718177954	22.174893362504523
38	19.915339272504053	30.713271055986652	25.996726110840036	23.37466356066926
39	20.203378559173974	29.680244912092927	26.715742212708353	23.400634316024743
40	21.450860182897078	28.16311995341004	28.281660711756096	22.10435915193679
41	19.073158830843813	29.85682637369556	25.723541309240865	25.34647348621976
42	20.09674106369918	29.417192640045332	26.02240174397557	24.46366455227992
43	20.40130719468623	27.19679143122472	27.406524955534916	24.995376418554137
44	19.041285631089355	26.825921174822533	27.80621881541876	26.326574378669353
45	20.383698235562623	26.36130042654998	26.37802401901374	26.876977318873656
46	20.839170195016763	27.88393433333858	27.496537232619268	23.780358239025386
47	18.85958871767428	28.349735570490925	28.890202729290287	23.900472982544503
48	18.79800654777832	27.261029701100213	28.344423370531853	25.596540380589616
49	20.300080273243825	26.396124848503927	29.45260730643917	23.851187571813075
50	18.883395243416807	25.69137298726646	29.146172068059116	26.279059701257612
51	18.181004359938928	25.60647616199455	27.09704012088206	29.115479357184455
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4252.0
1	17641.5
2	31031.0
3	19891.0
4	8751.0
5	5981.0
6	3211.0
7	2936.0
8	2661.0
9	2565.0
10	2469.0
11	2502.0
12	2535.0
13	2593.0
14	2651.0
15	2798.5
16	2946.0
17	3084.0
18	3222.0
19	3380.5
20	3539.0
21	3614.5
22	3690.0
23	3736.0
24	3782.0
25	5066.5
26	6674.0
27	6997.0
28	9416.0
29	11835.0
30	13320.0
31	14805.0
32	16822.0
33	18839.0
34	21128.0
35	23417.0
36	25135.0
37	26853.0
38	29406.5
39	31960.0
40	35885.5
41	39811.0
42	46548.5
43	53286.0
44	61442.0
45	69598.0
46	80939.0
47	92280.0
48	100545.5
49	108811.0
50	107167.0
51	105523.0
52	89618.0
53	73713.0
54	64980.5
55	56248.0
56	49735.5
57	43223.0
58	40586.5
59	37950.0
60	36023.0
61	34096.0
62	31056.0
63	28016.0
64	23694.5
65	19373.0
66	16205.5
67	13038.0
68	11029.5
69	9021.0
70	7452.0
71	5883.0
72	5358.0
73	4833.0
74	3755.5
75	2115.0
76	1552.0
77	1253.5
78	955.0
79	669.0
80	383.0
81	305.0
82	227.0
83	186.0
84	145.0
85	92.0
86	39.0
87	30.5
88	22.0
89	20.0
90	18.0
91	12.0
92	6.0
93	3.0
94	0.0
95	0.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1016528.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.498771862746047
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.01569695861454	21.243746151188247
2	11.8226830517258	6.975092602968272
3	4.721845233899915	4.17865905978025
4	2.3587474071475323	2.783206065811553
5	1.4007192627949798	2.065974898847147
6	0.9084315826008947	1.607856960483428
7	0.6186771205676067	1.2775150665427124
8	0.47021169481042724	1.109653400992637
9	0.386465885609982	1.0260242093108674
>10	4.446806011526618	32.147530586626786
>50	0.767179729388074	14.400230820133682
>100	0.07137339728345911	3.6253267411518997
>500	0.006088725834606179	1.2068789753997806
>1k	0.004059150556404119	2.4587602176788748
>5k	6.765250927340199E-4	1.07067477519964
>10k+	3.3826254636700994E-4	2.822869467884232
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	28290	2.7830025341161284	No Hit
GCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC	5601	0.5509931846442007	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGCT	5129	0.5045606220389404	Illumina Single End Adapter 1 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC	3771	0.37096863047550094	Illumina Single End Adapter 1 (95% over 21bp)
GAAACTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTC	3758	0.36968976752238997	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3744	0.36831253049596274	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCC	2760	0.27151244235279304	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2254	0.221735161254781	No Hit
TCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC	1616	0.15897250247902667	Illumina Single End Adapter 1 (95% over 21bp)
GAAAACTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTT	1168	0.1149009176333559	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTC	1143	0.11244156580045014	No Hit
CGCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTG	1127	0.1108675806273905	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCT	1127	0.1108675806273905	No Hit
ACTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC	1121	0.11027733618749311	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCT	1052	0.10348952512867328	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5851289880849322	0.0
2	0.0	0.0	0.0	1.9598082886059214	0.0
3	0.0	0.0	0.0	2.500865691845183	0.0
4	0.0	0.0	0.0	3.1163922685848298	0.0
5	0.0	0.0	0.0	4.931393921269262	0.0
6	0.0	0.0	0.0	5.99068594273842	0.0
7	0.0	0.0	0.0	7.113921111863126	0.0
8	0.0	0.0	0.0	8.80182346182299	0.0
9	0.0	0.0	0.0	9.404069538664945	0.0
10	0.0	0.0	0.0	10.919718886248091	0.0
11	0.0	0.0	0.0	13.06201108085562	0.0
12	0.0	0.0	0.0	14.64691577605339	0.0
13	0.0	0.0	0.0	15.236569971510868	0.0
14	0.0	0.0	0.0	15.481029543701698	0.0
15	0.0	0.0	0.0	15.910924244093621	0.0
16	0.0	0.0	0.0	16.819310437095684	0.0
17	0.0	0.0	0.0	17.884701651110447	0.0
18	0.0	0.0	0.0	19.161203626461838	0.0
19	9.837407331622937E-5	0.0	0.0	19.851887995215087	0.0
20	9.837407331622937E-5	0.0	0.0	20.49013898289078	0.0
21	9.837407331622937E-5	0.0	0.0	21.32966332457148	0.0
22	9.837407331622937E-5	0.0	0.0	22.18856735869548	0.0
23	9.837407331622937E-5	0.0	0.0	23.019926652290934	0.0
24	9.837407331622937E-5	0.0	0.0	23.66270284733918	0.0
25	9.837407331622937E-5	0.0	0.0	24.23297735035336	0.0
26	9.837407331622937E-5	0.0	0.0	24.772854264712826	0.0
27	9.837407331622937E-5	0.0	0.0	25.292761242189098	0.0
28	9.837407331622937E-5	0.0	0.0	25.850935734185384	0.0
29	9.837407331622937E-5	0.0	0.0	26.383631341192768	0.0
30	9.837407331622937E-5	0.0	0.0	26.987844899501045	0.0
31	9.837407331622937E-5	0.0	0.0	27.579269828278218	0.0
32	9.837407331622937E-5	0.0	0.0	28.146494735019598	0.0
33	1.9674814663245873E-4	0.0	0.0	28.70673508255552	0.0
34	2.951222199486881E-4	0.0	0.0	29.252219319094014	0.0
35	2.951222199486881E-4	0.0	0.0	29.875419073552326	0.0
36	2.951222199486881E-4	0.0	0.0	30.429363480396013	0.0
37	2.951222199486881E-4	0.0	0.0	30.957435505957534	0.0
38	2.951222199486881E-4	0.0	0.0	31.505575842475565	0.0
39	2.951222199486881E-4	0.0	0.0	32.060208867832465	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGC	20	7.032903E-4	45.0	26
CTATGCG	40	6.8139343E-9	45.0	1
GTCGCAA	20	7.032903E-4	45.0	9
GCGAACT	20	7.032903E-4	45.0	15
TAACGCG	25	3.890487E-5	45.0	1
CTACGGT	25	3.890487E-5	45.0	3
ACTCGGT	20	7.032903E-4	45.0	11
ATGCGCA	35	1.211738E-7	45.0	1
CAATGCG	20	7.032903E-4	45.0	1
CGCGTAT	20	7.032903E-4	45.0	24
CGATCTA	20	7.032903E-4	45.0	10
TATCGAA	20	7.032903E-4	45.0	31
TTAGCCG	30	2.165254E-6	44.999996	1
CCCTCGT	75	0.0	42.0	14
GCGTAAG	70	0.0	41.785713	1
TTTCGCG	55	6.184564E-11	40.909092	1
ACGCATT	45	1.9279469E-8	40.0	17
TATACGG	45	1.9279469E-8	40.0	2
CGTATGG	125	0.0	39.600002	2
CTACGAA	125	0.0	39.600002	11
>>END_MODULE