FastQCFastQC Report
Sat 18 Jun 2016
SRR3552399_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552399_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2095709
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC32470.1549356327619913Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC24220.11556948030475606Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGCT21660.10335404390590487Illumina Single End Adapter 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATTA207.0346164E-445.00000433
TTATGCG1000.040.51
ACGATAG700.038.571431
TTTAGCG1850.037.70271
ACAACGA4950.036.8181813
AGGGCGA20450.036.7481656
TACGGGT2450.036.7346954
TTAGCGG5350.036.5887832
GTATACG800.036.5625041
TAGGGCG8600.035.8430255
TCGATCA2350.035.42553317
ATCGTAG1150.035.2173921
CGTTAGG2950.035.0847472
TATGCGG2700.035.02
GCGATAT900.035.09
TACGGGA5900.034.7033924
CTCGATC2400.034.687516
TATAGCG1300.034.6153831
CACAACG5300.034.3867912
AAGGGCG8950.034.1899455
Produced by FastQC (version 0.11.5)