##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552399_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2095709
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.468474392198534	31.0	31.0	34.0	30.0	34.0
2	31.67683919857194	31.0	31.0	34.0	30.0	34.0
3	31.747148578357013	31.0	31.0	34.0	30.0	34.0
4	35.44754305106291	37.0	35.0	37.0	33.0	37.0
5	35.270127675168645	37.0	35.0	37.0	33.0	37.0
6	35.35959906647344	37.0	35.0	37.0	33.0	37.0
7	35.738125379048334	37.0	35.0	37.0	35.0	37.0
8	35.78347995833391	37.0	35.0	37.0	35.0	37.0
9	37.31719766437039	39.0	37.0	39.0	35.0	39.0
10	36.74359130967133	39.0	37.0	39.0	32.0	39.0
11	36.47605273442067	38.0	35.0	39.0	32.0	39.0
12	36.42597946566055	38.0	35.0	39.0	32.0	39.0
13	36.185677496255444	38.0	35.0	39.0	32.0	39.0
14	37.40968283287422	39.0	36.0	41.0	32.0	41.0
15	37.52273001642881	39.0	36.0	41.0	32.0	41.0
16	37.4989509516827	39.0	36.0	41.0	32.0	41.0
17	37.483323304905404	39.0	36.0	41.0	32.0	41.0
18	37.43644513622836	39.0	36.0	41.0	32.0	41.0
19	37.40747117085435	39.0	36.0	41.0	32.0	41.0
20	37.04177011216729	39.0	35.0	41.0	31.0	41.0
21	37.11461848949449	39.0	35.0	41.0	31.0	41.0
22	37.193467222787135	39.0	35.0	41.0	32.0	41.0
23	37.0474793971873	39.0	35.0	40.0	31.0	41.0
24	37.109070963573664	39.0	35.0	41.0	31.0	41.0
25	37.12504980414743	39.0	35.0	40.0	31.0	41.0
26	37.07380557128876	39.0	35.0	40.0	31.0	41.0
27	36.89151451847561	39.0	35.0	40.0	31.0	41.0
28	36.85527284560977	39.0	35.0	40.0	31.0	41.0
29	36.8877024434213	39.0	35.0	40.0	31.0	41.0
30	36.76645230802559	39.0	35.0	40.0	31.0	41.0
31	36.67630286456755	39.0	35.0	40.0	31.0	41.0
32	36.42759896531437	38.0	35.0	40.0	30.0	41.0
33	36.33549171187412	38.0	35.0	40.0	30.0	41.0
34	36.2368611290976	38.0	35.0	40.0	30.0	41.0
35	36.08164444586534	38.0	35.0	40.0	29.0	41.0
36	36.18012806167268	38.0	35.0	40.0	30.0	41.0
37	36.18821172214272	38.0	35.0	40.0	30.0	41.0
38	36.13129256017892	38.0	35.0	40.0	30.0	41.0
39	36.08316994391874	38.0	35.0	40.0	30.0	41.0
40	35.95770786879285	38.0	35.0	40.0	29.0	41.0
41	35.89419284833915	38.0	34.0	40.0	29.0	41.0
42	35.861889699380974	38.0	34.0	40.0	29.0	41.0
43	35.74331980251075	38.0	34.0	40.0	29.0	41.0
44	35.64565357117806	38.0	34.0	40.0	29.0	41.0
45	35.58152873323539	38.0	34.0	40.0	29.0	41.0
46	35.48569768035543	38.0	34.0	40.0	28.0	41.0
47	35.41652347725758	37.0	34.0	40.0	28.0	41.0
48	35.31721722815524	37.0	34.0	40.0	28.0	41.0
49	35.24050667339788	37.0	34.0	40.0	28.0	41.0
50	35.12179458121333	37.0	34.0	40.0	28.0	41.0
51	33.85634551361854	36.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	5.0
8	46.0
9	141.0
10	179.0
11	110.0
12	67.0
13	37.0
14	46.0
15	92.0
16	166.0
17	354.0
18	698.0
19	1202.0
20	2047.0
21	3288.0
22	5069.0
23	7370.0
24	10486.0
25	13959.0
26	18258.0
27	22845.0
28	28921.0
29	37153.0
30	47504.0
31	61500.0
32	80084.0
33	107915.0
34	165746.0
35	184619.0
36	205602.0
37	277643.0
38	398755.0
39	413760.0
40	41.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.718717150138694	19.816396264939453	26.199104932984497	11.265781651937363
2	31.644231140869273	23.6655947939337	26.508308166830414	18.181865898366613
3	32.85317761196808	24.163326110638454	25.95016769980947	17.033328577584005
4	28.801470051424122	26.407721682733627	25.20478749673738	19.58602076910487
5	25.680044319130186	29.989325808115534	24.382154201752247	19.948475671002033
6	26.387633015843328	35.285051502856554	23.47630324629994	14.851012235000184
7	84.00913485603202	5.4391139227822185	6.34186330258638	4.209887918599386
8	85.92261616474424	4.287618175996763	5.879251365528325	3.9105142937306656
9	80.24267682202063	6.4470305753327395	7.986652727072317	5.323639875574329
10	41.68255230091582	25.506785531769914	16.479530316470463	16.331131850843796
11	31.851941276198176	25.384535734684537	22.763465729259167	20.00005725985812
12	27.269148531594794	23.209520024010967	28.768163900617882	20.753167543776353
13	24.602127490028433	23.825683813926457	30.9316799231191	20.64050877292601
14	22.081548535602987	26.209984306027224	30.002495575483046	21.705971582886747
15	21.522883186549276	25.61648587661741	31.677203275836487	21.183427660996827
16	24.595304023602512	24.492093129341907	29.666809657256803	21.245793189798775
17	24.55646275317804	24.70853539303405	27.428808102651658	23.30619375113625
18	25.48516993532976	24.00061268048188	28.84875715092124	21.66546023326712
19	25.139034092996692	26.46865571508258	26.98471018638561	21.40760000553512
20	26.510216828767735	26.063733085080038	26.62168268590725	20.804367400244978
21	25.69598164630681	26.505874622860333	27.148091648220245	20.650052082612614
22	24.56323850305553	24.712734449296157	26.865848264238977	23.858178783409336
23	23.52110908527854	26.3568081255556	27.23216820655921	22.88991458260665
24	23.47902308956062	24.66983727225488	28.190030199803502	23.661109438380997
25	23.21114238665769	25.84729082138789	26.74135578937725	24.20021100257717
26	21.83370878304192	26.007188975186917	28.418163017861737	23.740939223909425
27	22.548645828213747	25.89648658282233	28.21197981208269	23.342887776881238
28	21.410987880473865	26.54576565735033	28.623773625059584	23.419472837116224
29	23.045231947756108	25.3302820191162	28.478333585435767	23.146152447691925
30	23.486514587664605	24.752482334140858	28.558926835739122	23.202076242455416
31	25.0034713788985	26.2323633672423	26.07967041225666	22.684494841602532
32	24.605133632579715	25.88116957077533	27.39674258210467	22.116954214540282
33	24.112221687266697	25.070703995640613	26.849433771578017	23.96764054551467
34	23.00095099081027	25.780487653581673	28.269048804008573	22.949512551599483
35	22.31664796973244	25.666254236633044	29.226767647607566	22.790330146026953
36	24.19510533189484	27.483443550607454	27.081097614220294	21.24035350327741
37	24.351377028012955	27.013244682348553	27.015344210479604	21.62003407915889
38	23.69694456625419	27.14370172576441	26.121326959038683	23.03802674894272
39	24.05047647359438	24.81083967287443	26.640960171474187	24.497723682057003
40	23.953516447178497	25.29363570991965	27.85319908441487	22.899648758486986
41	22.803070464458568	25.528973726791264	27.647349894474853	24.02060591427531
42	24.433974373350498	25.092844474113534	27.528392539231355	22.944788613304613
43	24.165282489124206	23.545015076043477	28.499376583294723	23.790325851537595
44	23.09313936238285	24.179883752944708	28.20487004636617	24.522106838306275
45	23.843577519588834	23.514285618852618	28.02316542993326	24.61897143162529
46	23.130310553612166	25.284808148459543	27.374649820180185	24.210231477748103
47	22.60022741706983	25.195816785631976	29.09478367464185	23.109172122656343
48	23.003527684425652	24.57211378106407	29.189071574345483	23.235286960164796
49	24.171151624581466	23.440945283911077	29.11759218479283	23.270310906714624
50	21.943552277534714	23.95456621124402	29.174374877428118	24.927506633793143
51	21.73121363700781	23.77916017920427	27.612850829957786	26.876775353830133
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	533.0
1	1018.5
2	1504.0
3	3032.0
4	4560.0
5	3451.0
6	2342.0
7	2331.0
8	2320.0
9	2531.0
10	2742.0
11	2968.5
12	3195.0
13	3368.0
14	3541.0
15	3764.5
16	3988.0
17	4236.0
18	4484.0
19	4607.0
20	4730.0
21	5549.0
22	6368.0
23	7405.5
24	8443.0
25	10814.5
26	15235.0
27	17284.0
28	20509.5
29	23735.0
30	29000.0
31	34265.0
32	37290.5
33	40316.0
34	46873.5
35	53431.0
36	57687.0
37	61943.0
38	67496.5
39	73050.0
40	79595.5
41	86141.0
42	96088.0
43	106035.0
44	115321.5
45	124608.0
46	141028.5
47	157449.0
48	173968.0
49	190487.0
50	191904.0
51	193321.0
52	175431.0
53	157541.0
54	144664.5
55	131788.0
56	122675.5
57	113563.0
58	108289.5
59	103016.0
60	98754.0
61	94492.0
62	87349.5
63	80207.0
64	71314.0
65	62421.0
66	54498.0
67	46575.0
68	38516.5
69	30458.0
70	26131.5
71	21805.0
72	17481.0
73	13157.0
74	10888.5
75	6453.5
76	4287.0
77	3062.5
78	1838.0
79	1367.5
80	897.0
81	688.5
82	480.0
83	387.5
84	295.0
85	200.0
86	105.0
87	86.5
88	68.0
89	63.0
90	58.0
91	38.0
92	18.0
93	13.0
94	8.0
95	8.5
96	9.0
97	5.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2095709.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.13851959065224
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.79804290991666	17.546194690801258
2	12.022209654512833	6.044411058457987
3	4.759310369638145	3.589260508954286
4	2.295351416891049	2.3080694624378806
5	1.3926273959250455	1.7504295537470385
6	0.9284699982551378	1.4004216744281777
7	0.6580287228093615	1.1579307557688507
8	0.4983076933971935	1.0021374170110464
9	0.39316779796633405	0.8895290752431263
>10	5.845664326203375	37.663564408940985
>50	1.2990134635384971	21.253014275993472
>100	0.10535991022881039	4.125911438745383
>500	0.00270646826286039	0.44223793540806394
>1k	0.0017398724546959653	0.8268877440624406
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	3247	0.1549356327619913	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	2422	0.11556948030475606	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGCT	2166	0.10335404390590487	Illumina Single End Adapter 1 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11848018975916981	0.0
2	0.0	0.0	0.0	0.5531302294354798	0.0
3	0.0	0.0	0.0	0.7635602080250645	0.0
4	0.0	0.0	0.0	1.0314886274764292	0.0
5	0.0	0.0	0.0	1.8051170272208594	0.0
6	0.0	0.0	0.0	2.3521872550053464	0.0
7	0.0	0.0	0.0	2.9015001605661856	0.0
8	0.0	0.0	0.0	3.834501832076877	0.0
9	0.0	0.0	0.0	4.157447431871505	0.0
10	0.0	0.0	0.0	5.097606585647148	0.0
11	0.0	0.0	0.0	6.515646971979411	0.0
12	0.0	0.0	0.0	7.52146409639888	0.0
13	0.0	0.0	0.0	7.928295388338744	0.0
14	0.0	0.0	0.0	8.093824094852863	0.0
15	0.0	0.0	0.0	8.400689217825567	0.0
16	0.0	0.0	0.0	9.080220584060097	0.0
17	0.0	0.0	0.0	9.919220655157753	0.0
18	0.0	0.0	0.0	10.778118526951976	0.0
19	0.0	0.0	0.0	11.305577253330496	0.0
20	0.0	0.0	0.0	11.822013457020988	0.0
21	4.7716548433012405E-5	0.0	0.0	12.484700881658666	0.0
22	9.543309686602481E-5	0.0	0.0	13.178213196584068	0.0
23	9.543309686602481E-5	0.0	0.0	13.85636078291404	0.0
24	9.543309686602481E-5	0.0	0.0	14.405339672635847	0.0
25	1.9086619373204962E-4	0.0	0.0	14.908319809668232	0.0
26	1.9086619373204962E-4	0.0	0.0	15.375178519536824	0.0
27	1.9086619373204962E-4	0.0	0.0	15.819228719254438	0.0
28	1.9086619373204962E-4	0.0	0.0	16.273919709272615	0.0
29	1.9086619373204962E-4	0.0	0.0	16.769837797136912	0.0
30	1.9086619373204962E-4	0.0	0.0	17.329075744771817	0.0
31	1.9086619373204962E-4	0.0	0.0	17.843841869267155	0.0
32	1.9086619373204962E-4	0.0	0.0	18.350830196367912	0.0
33	1.9086619373204962E-4	0.0	0.0	18.870892857739314	0.0
34	1.9086619373204962E-4	0.0	0.0	19.383177721716134	0.0
35	1.9086619373204962E-4	0.0	0.0	19.93907551096073	0.0
36	1.9086619373204962E-4	0.0	0.0	20.443773443736703	0.0
37	2.3858274216506203E-4	0.0	0.0	20.96474271952833	0.0
38	2.8629929059807447E-4	0.0	0.0	21.5194475950621	0.0
39	2.8629929059807447E-4	0.0	0.0	22.075631683597294	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGATTA	20	7.0346164E-4	45.000004	33
TTATGCG	100	0.0	40.5	1
ACGATAG	70	0.0	38.57143	1
TTTAGCG	185	0.0	37.7027	1
ACAACGA	495	0.0	36.81818	13
AGGGCGA	2045	0.0	36.748165	6
TACGGGT	245	0.0	36.734695	4
TTAGCGG	535	0.0	36.588783	2
GTATACG	80	0.0	36.562504	1
TAGGGCG	860	0.0	35.843025	5
TCGATCA	235	0.0	35.425533	17
ATCGTAG	115	0.0	35.217392	1
CGTTAGG	295	0.0	35.084747	2
TATGCGG	270	0.0	35.0	2
GCGATAT	90	0.0	35.0	9
TACGGGA	590	0.0	34.703392	4
CTCGATC	240	0.0	34.6875	16
TATAGCG	130	0.0	34.615383	1
CACAACG	530	0.0	34.38679	12
AAGGGCG	895	0.0	34.189945	5
>>END_MODULE