##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552398_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	879731
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.276070753446223	31.0	31.0	34.0	30.0	34.0
2	31.474679191707466	31.0	31.0	34.0	30.0	34.0
3	31.550359143874662	31.0	31.0	34.0	30.0	34.0
4	35.26894584821951	37.0	35.0	37.0	33.0	37.0
5	35.09380708421097	37.0	35.0	37.0	32.0	37.0
6	35.19772521373011	37.0	35.0	37.0	32.0	37.0
7	35.57409367181559	37.0	35.0	37.0	35.0	37.0
8	35.61309422994074	37.0	35.0	37.0	35.0	37.0
9	37.112449146386794	39.0	37.0	39.0	34.0	39.0
10	36.56158189264673	38.0	35.0	39.0	32.0	39.0
11	36.3001144668086	38.0	35.0	39.0	32.0	39.0
12	36.22537798486128	38.0	35.0	39.0	32.0	39.0
13	35.934885777584284	38.0	35.0	39.0	31.0	39.0
14	37.10993133128195	39.0	36.0	40.0	32.0	41.0
15	37.23906284989389	39.0	36.0	40.0	32.0	41.0
16	37.22192579322543	39.0	36.0	40.0	32.0	41.0
17	37.254118588523085	39.0	36.0	40.0	32.0	41.0
18	37.209418560901	39.0	36.0	40.0	32.0	41.0
19	37.1675284831386	39.0	35.0	40.0	32.0	41.0
20	36.7629388983678	39.0	35.0	40.0	31.0	41.0
21	36.843296416745574	39.0	35.0	40.0	31.0	41.0
22	36.94554244422443	39.0	35.0	40.0	31.0	41.0
23	36.816737161700566	39.0	35.0	40.0	31.0	41.0
24	36.86672062255394	39.0	35.0	40.0	31.0	41.0
25	36.86245568247566	39.0	35.0	40.0	31.0	41.0
26	36.8012324221836	39.0	35.0	40.0	31.0	41.0
27	36.58502883267726	38.0	35.0	40.0	31.0	41.0
28	36.552855361468445	38.0	35.0	40.0	30.0	41.0
29	36.608314359730414	38.0	35.0	40.0	30.0	41.0
30	36.49752594827282	38.0	35.0	40.0	30.0	41.0
31	36.38252829558126	38.0	35.0	40.0	30.0	41.0
32	36.111595476344476	38.0	35.0	40.0	30.0	41.0
33	36.03012398108058	38.0	35.0	40.0	29.0	41.0
34	35.96610213803992	38.0	35.0	40.0	29.0	41.0
35	35.80938036740776	38.0	35.0	40.0	29.0	41.0
36	35.86586695251162	38.0	35.0	40.0	29.0	41.0
37	35.88234130660395	38.0	35.0	40.0	29.0	41.0
38	35.83887802066768	38.0	35.0	40.0	29.0	41.0
39	35.80710467176898	38.0	34.0	40.0	29.0	41.0
40	35.70405157940325	38.0	34.0	40.0	29.0	41.0
41	35.62190260431882	38.0	34.0	40.0	28.0	41.0
42	35.63903966098728	38.0	34.0	40.0	29.0	41.0
43	35.55084338280679	38.0	34.0	40.0	28.0	41.0
44	35.45000119354666	38.0	34.0	40.0	28.0	41.0
45	35.35676928515649	38.0	34.0	40.0	28.0	41.0
46	35.22709896547922	38.0	34.0	40.0	27.0	41.0
47	35.137295377791624	37.0	34.0	40.0	27.0	41.0
48	35.093259189456774	37.0	34.0	40.0	27.0	41.0
49	35.02859737806216	37.0	34.0	40.0	27.0	41.0
50	34.920953109530075	37.0	34.0	40.0	27.0	41.0
51	33.71715899519285	36.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	14.0
9	53.0
10	59.0
11	43.0
12	22.0
13	24.0
14	23.0
15	34.0
16	98.0
17	191.0
18	400.0
19	661.0
20	1201.0
21	1762.0
22	2737.0
23	3851.0
24	5396.0
25	7177.0
26	9034.0
27	10875.0
28	13792.0
29	17223.0
30	22031.0
31	28024.0
32	35757.0
33	47866.0
34	70880.0
35	80530.0
36	85323.0
37	113325.0
38	159727.0
39	161580.0
40	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.04380429926875	19.61895170228172	24.51328872121137	11.823955277238156
2	30.913767958614624	24.42576196587366	25.975894904237773	18.684575171273945
3	32.54585776788586	23.259496368776365	27.092713568124804	17.10193229521297
4	28.068239041252387	26.926412732983152	26.512081534014374	18.49326669175009
5	23.717590945413995	30.622315230451125	25.609305571816837	20.05078825231804
6	24.56625945885731	36.31473711850554	24.34551016162895	14.773493261008195
7	80.51063336406243	6.8796029695440994	7.776013349535256	4.833750316858222
8	81.84206308519309	5.421657302061653	7.57151902115533	5.164760591589929
9	76.65138548033433	7.847057793802878	9.584861736144344	5.916694989718448
10	39.53333462160592	25.604076700718743	18.355724647647975	16.50686403002736
11	32.179268435464934	26.683838582475776	21.66548638163257	19.47140660042672
12	26.883786066422577	23.744530998680276	29.100031714239922	20.271651220657226
13	24.52295076563177	25.87666002448476	29.762165934814167	19.83822327506931
14	20.897297014655617	28.961807643472838	28.96658183012762	21.174313511743932
15	20.319847771648377	26.703617355759885	32.30714843514665	20.669386437445084
16	22.661017970265913	25.34524758136294	30.63561475041803	21.358119697953125
17	21.9280666476457	25.30364395479982	28.251931556350744	24.51635784120373
18	23.11286063580799	25.538261127549216	29.696918717198777	21.651959519444013
19	24.111688686655352	27.16091623462172	27.945701583779588	20.78169349494334
20	24.837137715960903	27.075890243722228	27.916601779407568	20.1703702609093
21	23.350774270771407	29.111285154211913	27.90011946833748	19.6378211066792
22	21.666282079408365	26.788643346659374	26.568462404985162	24.9766121689471
23	21.63627290614972	28.35650897831269	26.879239221989447	23.12797889354814
24	21.448147217729055	25.864156202293653	29.00056949226525	23.68712708771204
25	20.83455056147845	28.49291431130652	26.660649675866825	24.011885451348196
26	20.653927166372448	29.154821189659113	27.154209639082854	23.037042004885585
27	22.28544862008955	28.45096967141092	27.395306065149462	21.868275643350067
28	19.158583703427524	28.85075096819369	29.558467304210033	22.432198024168752
29	22.024687091849668	27.97252796593504	27.513524020410784	22.489260921804508
30	23.456602074952457	27.34733685637996	26.60892932044	22.58713174822758
31	23.257677631003112	29.992804618684577	23.926291104894563	22.823226645417748
32	22.39264047760054	29.153457136329173	25.4958618032103	22.958040582859987
33	23.564930643571728	27.2499207144002	25.515185892051097	23.669962749976982
34	20.581632339885715	28.016177672493072	27.019509372751443	24.382680614869773
35	22.229522433562078	26.485027809637263	26.565052271660317	24.720397485140346
36	23.86047553172504	29.5688113752954	25.15780391960724	21.412909173372316
37	21.752558452526966	29.563241490864822	26.32963940113512	22.354560655473094
38	21.69890568821606	28.572825102218747	26.94323605738572	22.785033152179473
39	23.5825496657501	24.671973591927532	27.865108766202397	23.880367976119974
40	23.535262483645567	23.65961867889162	27.47533052717251	25.3297883102903
41	19.604174457874056	25.60612278071365	26.748858457869506	28.04084430354279
42	21.169652996199975	26.15322183712976	27.99298876588412	24.68413640078615
43	22.732517098976846	24.142948242133105	29.799222716944158	23.325311941945888
44	22.265215162362132	25.168602675135922	27.2483293188486	25.317852843653345
45	22.95724488508419	24.719488116253718	27.29573017206396	25.02753682659813
46	23.060685595937848	25.92712999769248	26.99586578169918	24.016318624670497
47	20.61016378870359	25.81732370463244	29.338854718089962	24.233657788574007
48	20.39282462480008	25.17633231067224	29.852761810144234	24.57808125438344
49	22.32011830889215	23.95357217149333	30.18195334710269	23.544356172511822
50	20.770212712749693	23.655753861123458	29.1402712874731	26.433762138653748
51	20.432723184700777	23.5736833191055	27.023828874962913	28.96976462123081
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1131.0
1	1273.5
2	1416.0
3	2228.0
4	3040.0
5	2273.0
6	1506.0
7	1516.0
8	1526.0
9	1581.5
10	1637.0
11	1705.0
12	1773.0
13	1801.5
14	1830.0
15	1938.5
16	2047.0
17	2237.5
18	2428.0
19	2475.5
20	2523.0
21	2715.5
22	2908.0
23	3343.5
24	3779.0
25	4834.5
26	7099.0
27	8308.0
28	9558.5
29	10809.0
30	12881.0
31	14953.0
32	15681.0
33	16409.0
34	18545.5
35	20682.0
36	22734.5
37	24787.0
38	26779.5
39	28772.0
40	32951.0
41	37130.0
42	43310.5
43	49491.0
44	54085.5
45	58680.0
46	65550.0
47	72420.0
48	84452.0
49	96484.0
50	94120.5
51	91757.0
52	80969.5
53	70182.0
54	62002.0
55	53822.0
56	48287.5
57	42753.0
58	38361.0
59	33969.0
60	32543.0
61	31117.0
62	28791.0
63	26465.0
64	23105.5
65	19746.0
66	16225.5
67	12705.0
68	10606.5
69	8508.0
70	7592.0
71	6676.0
72	5374.0
73	4072.0
74	3251.5
75	1904.0
76	1377.0
77	1126.5
78	876.0
79	682.0
80	488.0
81	370.5
82	253.0
83	170.5
84	88.0
85	74.0
86	60.0
87	36.0
88	12.0
89	9.0
90	6.0
91	6.0
92	6.0
93	4.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	879731.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.52628937603717
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.30171917825913	22.794049211987737
2	11.684367370961906	7.367294938257434
3	4.411444667153129	4.1722944342913655
4	2.142640351141394	2.7019799895542986
5	1.2986041290583534	2.047008477880518
6	0.812520501411968	1.536945387088591
7	0.6082317948787493	1.3422704101137752
8	0.468098483714548	1.1805926603255248
9	0.3898418777794771	1.1061241064796157
>10	5.300923950465736	40.025838378447745
>50	0.5197111620541507	10.280592451572517
>100	0.055304002966499635	3.0079933814974833
>500	0.004028768428023119	0.8590637886584455
>1k	0.0025637617269238027	1.5779523838449347
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGCCGTCTTCTGCT	3912	0.4446813855599041	No Hit
CCTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGCCGTCTTCTGC	2222	0.25257720826025226	No Hit
GCTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGCCGTCTTCTGC	2104	0.2391640171825251	No Hit
TCTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGCCGTCTTCTGC	1531	0.1740304706779686	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGCC	1376	0.15641144849959818	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1367	0.15538840850214442	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1154	0.1311764618957386	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	985	0.1119660441657734	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5039040343013944	0.0
2	0.0	0.0	0.0	1.354618627739616	0.0
3	0.0	0.0	0.0	1.7285965823643819	0.0
4	0.0	0.0	0.0	2.1793025367981804	0.0
5	0.0	0.0	0.0	3.257132009671138	0.0
6	0.0	0.0	0.0	3.93324777687725	0.0
7	0.0	0.0	0.0	4.664380361724209	0.0
8	0.0	0.0	0.0	5.970916109583498	0.0
9	0.0	0.0	0.0	6.416847877362512	0.0
10	0.0	0.0	0.0	7.8173896338767195	0.0
11	0.0	0.0	0.0	10.021927157278759	0.0
12	0.0	0.0	0.0	11.640262762139791	0.0
13	0.0	0.0	0.0	12.272274138344562	0.0
14	0.0	0.0	0.0	12.525988057713096	0.0
15	0.0	0.0	0.0	12.976921354368551	0.0
16	0.0	0.0	0.0	13.967451414125454	0.0
17	0.0	0.0	0.0	15.104276193518245	0.0
18	0.0	0.0	0.0	16.420019301354618	0.0
19	0.0	0.0	0.0	17.135465272907286	0.0
20	0.0	0.0	0.0	17.831928168951645	0.0
21	3.4101333248458905E-4	0.0	0.0	18.66616045131978	0.0
22	3.4101333248458905E-4	0.0	0.0	19.515510991428062	0.0
23	3.4101333248458905E-4	0.0	0.0	20.352926064899385	0.0
24	3.4101333248458905E-4	0.0	0.0	21.03824919208258	0.0
25	3.4101333248458905E-4	0.0	0.0	21.652527874998153	0.0
26	3.4101333248458905E-4	0.0	0.0	22.20474213140153	0.0
27	3.4101333248458905E-4	0.0	0.0	22.746612316719542	0.0
28	3.4101333248458905E-4	0.0	0.0	23.302123035336937	0.0
29	3.4101333248458905E-4	0.0	0.0	23.881618358339082	0.0
30	3.4101333248458905E-4	0.0	0.0	24.504877059010084	0.0
31	3.4101333248458905E-4	0.0	0.0	25.112790159719278	0.0
32	3.4101333248458905E-4	0.0	0.0	25.685237873850074	0.0
33	3.4101333248458905E-4	0.0	0.0	26.252456716882776	0.0
34	3.4101333248458905E-4	0.0	0.0	26.789439044435174	0.0
35	4.5468444331278537E-4	0.0	0.0	27.361091060790173	0.0
36	4.5468444331278537E-4	0.0	0.0	27.95127146821017	0.0
37	4.5468444331278537E-4	0.0	0.0	28.549522524498965	0.0
38	4.5468444331278537E-4	0.0	0.0	29.135156087485832	0.0
39	4.5468444331278537E-4	0.0	0.0	29.744092228192482	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGATCG	30	2.1649194E-6	45.000004	1
GTCGATT	25	3.890057E-5	45.0	35
TCGATAG	25	3.890057E-5	45.0	1
AGCGGTA	20	7.032385E-4	45.0	10
GATATCC	20	7.032385E-4	45.0	29
GCGATAT	40	6.8121153E-9	45.0	9
CCGGTCA	20	7.032385E-4	45.0	34
CAATACG	25	3.890057E-5	45.0	1
CGATACG	20	7.032385E-4	45.0	10
TCGGTAA	20	7.032385E-4	45.0	28
TCGAACG	35	1.2115015E-7	45.0	1
CGAGACA	125	0.0	43.199997	22
GCTAGCG	60	3.6379788E-12	41.250004	1
ATAACGG	50	1.0804797E-9	40.5	2
TATTGCG	45	1.9274012E-8	40.0	1
AATACGG	90	0.0	40.0	2
TCAACGT	45	1.9274012E-8	40.0	24
CGTATGG	40	3.4569894E-7	39.375	2
AACGAGC	80	0.0	39.375	15
CCTCGTG	150	0.0	39.0	15
>>END_MODULE