FastQCFastQC Report
Sat 18 Jun 2016
SRR3552397_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552397_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1599243
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGCT48180.3012675372035394No Hit
GCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC46910.29332627999622324No Hit
CCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC37460.23423582282367345No Hit
TCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC34190.21378864875444195No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCC28120.17583319107852902No Hit
ACTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC19850.1241212248545093No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16200.10129792658151388No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGCG207.034115E-445.0000041
CTAGACG406.8175723E-945.0000041
ACGATCG207.034115E-445.00000433
TCGACGT302.1660362E-645.00000426
GCGCGAC453.8562575E-1045.09
CGCTAAG603.6379788E-1241.25000421
ACGGATC603.6379788E-1241.2500045
TTTCGCG451.9290383E-840.01
CGTGCGG1700.039.7058832
GTCGCTC403.459354E-739.3750041
CGCTCCG403.459354E-739.37500421
ACGTGCG403.459354E-739.3750041
CTATCGT356.249824E-638.57142612
CGGCCGC356.249824E-638.57142622
TAGCGCG700.038.5714261
GTACGGC356.249824E-638.5714269
CGTTTTT20700.038.3695681
TTGCGCG950.037.8947371
CAACGCG301.1401214E-437.5000041
ACCGCTA301.1401214E-437.50000426