##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552397_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1599243
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.1629208319186	31.0	31.0	34.0	28.0	34.0
2	31.35048957538035	31.0	31.0	34.0	28.0	34.0
3	31.432688465730347	31.0	31.0	34.0	28.0	34.0
4	35.139931830247185	37.0	35.0	37.0	32.0	37.0
5	34.97999991245858	35.0	35.0	37.0	32.0	37.0
6	35.075457575865585	36.0	35.0	37.0	32.0	37.0
7	35.58776996366406	37.0	35.0	37.0	35.0	37.0
8	35.66567307157199	37.0	35.0	37.0	35.0	37.0
9	37.152966122096515	39.0	37.0	39.0	34.0	39.0
10	36.4246233999461	38.0	35.0	39.0	32.0	39.0
11	36.1859023300399	38.0	35.0	39.0	31.0	39.0
12	36.263438389287934	38.0	35.0	39.0	32.0	39.0
13	36.06949538000166	38.0	35.0	39.0	31.0	39.0
14	37.221330967213866	39.0	36.0	40.0	32.0	41.0
15	37.335213597933524	39.0	36.0	40.0	32.0	41.0
16	37.295145265603786	39.0	36.0	40.0	32.0	41.0
17	37.33312073274668	39.0	36.0	40.0	32.0	41.0
18	37.30646874802641	39.0	36.0	40.0	32.0	41.0
19	37.269349310892714	39.0	36.0	40.0	32.0	41.0
20	36.91105041572794	39.0	35.0	40.0	31.0	41.0
21	37.01386718591233	39.0	35.0	40.0	31.0	41.0
22	37.11556280065006	39.0	35.0	40.0	31.0	41.0
23	36.96034873999761	39.0	35.0	40.0	31.0	41.0
24	37.01319624347269	39.0	35.0	40.0	31.0	41.0
25	37.051571274659324	39.0	35.0	40.0	31.0	41.0
26	37.01788283581669	39.0	35.0	40.0	31.0	41.0
27	36.80904965661879	39.0	35.0	40.0	31.0	41.0
28	36.78769392768954	39.0	35.0	40.0	31.0	41.0
29	36.83767132324481	39.0	35.0	40.0	31.0	41.0
30	36.70042763982709	39.0	35.0	40.0	30.0	41.0
31	36.62849610722073	39.0	35.0	40.0	30.0	41.0
32	36.3691333962381	38.0	35.0	40.0	30.0	41.0
33	36.29373772466098	38.0	35.0	40.0	30.0	41.0
34	36.288786632175345	38.0	35.0	40.0	30.0	41.0
35	36.139784260428215	38.0	35.0	40.0	30.0	41.0
36	36.20882442505611	38.0	35.0	40.0	30.0	41.0
37	36.218041911079176	38.0	35.0	40.0	30.0	41.0
38	36.17658041961103	38.0	35.0	40.0	30.0	41.0
39	36.1378020726056	38.0	35.0	40.0	30.0	41.0
40	36.07842898171197	38.0	35.0	40.0	29.0	41.0
41	36.03568188199041	38.0	35.0	40.0	29.0	41.0
42	36.04399956729527	38.0	35.0	40.0	29.0	41.0
43	35.93839272705899	38.0	35.0	40.0	29.0	41.0
44	35.85146472424766	38.0	34.0	40.0	28.0	41.0
45	35.778405783236195	38.0	34.0	40.0	28.0	41.0
46	35.65165018699472	38.0	34.0	40.0	28.0	41.0
47	35.563548503885904	38.0	34.0	40.0	28.0	41.0
48	35.505626724644095	38.0	34.0	40.0	28.0	41.0
49	35.43465752234026	38.0	34.0	40.0	28.0	41.0
50	35.31569623878298	38.0	34.0	40.0	27.0	41.0
51	34.120638327008464	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	23.0
9	64.0
10	87.0
11	63.0
12	40.0
13	24.0
14	34.0
15	54.0
16	138.0
17	265.0
18	536.0
19	903.0
20	1547.0
21	2573.0
22	3961.0
23	5628.0
24	8155.0
25	11173.0
26	14747.0
27	18695.0
28	23620.0
29	30197.0
30	39459.0
31	49943.0
32	63624.0
33	82751.0
34	115247.0
35	135666.0
36	159966.0
37	213805.0
38	310857.0
39	305362.0
40	34.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.45830245935108	21.302141075496344	26.62247075647666	10.617085708675917
2	30.083858425517572	24.63734404340053	27.39827530900557	17.88052222207632
3	30.692896576692846	24.098776733742152	27.717801484827508	17.49052520473749
4	27.110076454922737	27.123207667627746	27.719302194850936	18.047413682598577
5	24.860324541048485	30.591098413436857	26.160564717181817	18.38801232833284
6	24.31200261623781	36.097203489400925	25.19398240292438	14.396811491436887
7	82.62509199665091	5.595584911111069	7.397625001328753	4.3816980909092615
8	84.226036943729	4.106818038284364	6.8062201929287784	4.860924825057856
9	78.2110661106536	7.0120050548915955	9.33347840196893	5.44345043248587
10	35.868282681243564	30.890177415189562	18.663330088047907	14.578209815518967
11	25.624561120480127	27.603372345540983	27.464431609205104	19.307634924773783
12	22.355264334438232	25.119822315933227	32.51438336763081	20.01052998199773
13	23.11612431631716	25.370378360261697	33.98595460477238	17.52754271864876
14	20.4275397797583	28.82688872172647	30.833525611805086	19.91204588671015
15	19.722081009577657	28.471970801185307	33.015182808366205	18.790765380870823
16	21.77267619742591	27.6361378477192	30.430710029682796	20.160475925172097
17	21.956263056958825	27.560101873198757	28.84708577745846	21.636549292383958
18	21.907427451613046	27.040293438833245	31.248034226193266	19.80424488336044
19	21.284257614383804	29.081071481944896	29.908400411944903	19.726270491726396
20	22.041803528294324	28.760607362358314	29.947293813385457	19.2502952959619
21	21.917307125933956	29.29117088522507	30.246560403891092	18.544961584949878
22	20.2590850796283	27.180672355608248	28.970831824807114	23.589410739956342
23	19.216404261266113	28.553822027046543	29.49801875012115	22.731754961566192
24	19.729459500526186	28.100607599970733	29.95848660897687	22.211446290526204
25	18.873366961743777	29.315244775184258	28.413505639855856	23.39788262321611
26	19.226784172261503	29.63383300724155	29.02854663112485	22.110836189372097
27	21.51236553794514	28.424823494615893	28.84708577745846	21.215725189980507
28	19.401491830822458	28.10892403468391	29.778213817412365	22.71137031708127
29	20.252394414107176	27.052049000683446	28.842896295309718	23.852660289899656
30	22.633583514200158	28.378113895136636	27.228945194695243	21.759357395967967
31	21.99671969800712	29.568239473300807	25.256449457649648	23.178591371042426
32	20.73856193211413	30.598226786048148	25.28727654271427	23.375934739123448
33	21.714085976927834	30.084733839697908	25.201173305119983	23.000006878254272
34	19.85770767794513	28.338157490762818	26.688251879170334	25.11588295212172
35	19.895663135621042	29.303114035828205	25.914135625417778	24.88708720313298
36	22.425797705539434	30.10161682746149	25.592045736639147	21.880539730359928
37	21.55263459024051	30.159269104194923	26.838510470266247	21.449585835298326
38	19.84288816646376	33.262925021400754	25.652824492588056	21.241362319547434
39	19.87784220409281	31.21977085408534	26.255171978242203	22.647214963579643
40	20.15428549632545	28.69945342890355	27.13458805197209	24.011673022798913
41	19.15606321240737	29.64252461946058	25.423591036509148	25.777821131622897
42	20.920022785780525	29.199002277952758	26.6514844835963	23.22949045267042
43	21.85927967169467	27.932403018177975	27.71436235769048	22.49395495243687
44	20.99881006201059	28.03501406602999	26.560566468010176	24.405609403949242
45	21.408941605497102	26.985642582146678	26.480528600093923	25.124887212262305
46	21.580647844011196	28.415444056969452	26.63072466160552	23.37318343741383
47	19.303320383456423	28.628044643622015	28.243925407208287	23.824709565713277
48	19.897914200656185	28.33734460616679	28.10154554373538	23.663195649441644
49	21.325402080859508	26.90866866386159	28.71270970077718	23.053219554501723
50	20.147032064545538	25.99129713245579	27.954350902270637	25.907319900728034
51	19.46145770217534	25.936020979926127	26.264613945472952	28.33790737242558
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	632.0
1	816.0
2	1000.0
3	2049.5
4	3099.0
5	2389.5
6	1680.0
7	1772.5
8	1865.0
9	2075.0
10	2285.0
11	2533.0
12	2781.0
13	3115.5
14	3450.0
15	3919.5
16	4389.0
17	4513.0
18	4637.0
19	5075.5
20	5514.0
21	5953.0
22	6392.0
23	7505.0
24	8618.0
25	10260.0
26	14618.0
27	17334.0
28	19736.5
29	22139.0
30	25549.0
31	28959.0
32	34468.0
33	39977.0
34	45823.5
35	51670.0
36	56006.0
37	60342.0
38	63752.0
39	67162.0
40	74948.0
41	82734.0
42	92477.0
43	102220.0
44	114145.5
45	126071.0
46	145378.0
47	164685.0
48	180180.5
49	195676.0
50	187191.5
51	178707.0
52	152735.0
53	126763.0
54	105840.0
55	84917.0
56	72505.0
57	60093.0
58	51857.0
59	43621.0
60	37591.0
61	31561.0
62	27192.5
63	22824.0
64	18330.0
65	13836.0
66	11158.5
67	8481.0
68	6574.5
69	4668.0
70	3678.0
71	2688.0
72	2197.0
73	1706.0
74	1299.0
75	719.0
76	546.0
77	488.5
78	431.0
79	317.0
80	203.0
81	124.0
82	45.0
83	30.0
84	15.0
85	14.5
86	14.0
87	12.0
88	10.0
89	7.5
90	5.0
91	3.5
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1599243.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.883972412282034
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.54339644935747	23.902904991587036
2	12.689914435151625	8.599694212697946
3	4.917532237072667	4.998765799724334
4	2.362660692339977	3.202253188753238
5	1.4070620831317093	2.3838426403601463
6	0.939288991072588	1.909610535639828
7	0.6888322776134553	1.6338261723940606
8	0.5424642003411918	1.4704673599209264
9	0.4726805623452878	1.4414665620896723
>10	5.0270558823376295	37.33301730916419
>50	0.35650148119546493	7.647332089278572
>100	0.04736836961435592	2.9423383907680223
>500	0.0029956219579356028	0.7049423060142964
>1k	0.002246716468451702	1.8295384416078377
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGCT	4818	0.3012675372035394	No Hit
GCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC	4691	0.29332627999622324	No Hit
CCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC	3746	0.23423582282367345	No Hit
TCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC	3419	0.21378864875444195	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCC	2812	0.17583319107852902	No Hit
ACTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC	1985	0.1241212248545093	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1620	0.10129792658151388	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.34797713668279306	0.0
2	0.0	0.0	0.0	1.324751773182687	0.0
3	0.0	0.0	0.0	1.8708226329582183	0.0
4	0.0	0.0	0.0	2.5495187410543614	0.0
5	0.0	0.0	0.0	4.029594001662036	0.0
6	0.0	0.0	0.0	4.983482810304626	0.0
7	0.0	0.0	0.0	5.975827313297604	0.0
8	0.0	0.0	0.0	7.680071133655111	0.0
9	0.0	0.0	0.0	8.311119698507357	0.0
10	0.0	0.0	0.0	9.9183176040164	0.0
11	0.0	0.0	0.0	12.229598628851276	0.0
12	0.0	0.0	0.0	13.928715023295396	0.0
13	0.0	0.0	0.0	14.58840213776143	0.0
14	0.0	0.0	0.0	14.853214927312484	0.0
15	0.0	0.0	0.0	15.295674265887047	0.0
16	0.0	0.0	0.0	16.33923049842957	0.0
17	0.0	0.0	0.0	17.69599741877876	0.0
18	0.0	0.0	0.0	19.18101251654689	0.0
19	0.0	0.0	0.0	20.075623279264	0.0
20	0.0	0.0	0.0	20.911893939820278	0.0
21	6.252958430957647E-5	0.0	0.0	21.91261740711074	0.0
22	6.252958430957647E-5	0.0	0.0	22.998005931556367	0.0
23	6.252958430957647E-5	0.0	0.0	24.079642680943422	0.0
24	1.2505916861915294E-4	0.0	0.0	24.902844658378996	0.0
25	4.3770709016703526E-4	0.0	0.0	25.627500010942676	0.0
26	4.3770709016703526E-4	0.0	0.0	26.315012790426472	0.0
27	4.3770709016703526E-4	0.0	0.0	26.994896960624494	0.0
28	4.3770709016703526E-4	0.0	0.0	27.71336188434153	0.0
29	4.3770709016703526E-4	0.0	0.0	28.459527413907704	0.0
30	4.3770709016703526E-4	0.0	0.0	29.278477379610226	0.0
31	4.3770709016703526E-4	0.0	0.0	30.01201193314587	0.0
32	4.3770709016703526E-4	0.0	0.0	30.72778808473759	0.0
33	4.3770709016703526E-4	0.0	0.0	31.409110435374735	0.0
34	5.627662587861883E-4	0.0	0.0	32.11269331802609	0.0
35	6.252958430957646E-4	0.0	0.0	32.80833494347013	0.0
36	8.128845960244941E-4	0.0	0.0	33.50804099189429	0.0
37	8.128845960244941E-4	0.0	0.0	34.18154714449274	0.0
38	8.754141803340705E-4	0.0	0.0	34.886318089245975	0.0
39	8.754141803340705E-4	0.0	0.0	35.591901918595234	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGCG	20	7.034115E-4	45.000004	1
CTAGACG	40	6.8175723E-9	45.000004	1
ACGATCG	20	7.034115E-4	45.000004	33
TCGACGT	30	2.1660362E-6	45.000004	26
GCGCGAC	45	3.8562575E-10	45.0	9
CGCTAAG	60	3.6379788E-12	41.250004	21
ACGGATC	60	3.6379788E-12	41.250004	5
TTTCGCG	45	1.9290383E-8	40.0	1
CGTGCGG	170	0.0	39.705883	2
GTCGCTC	40	3.459354E-7	39.375004	1
CGCTCCG	40	3.459354E-7	39.375004	21
ACGTGCG	40	3.459354E-7	39.375004	1
CTATCGT	35	6.249824E-6	38.571426	12
CGGCCGC	35	6.249824E-6	38.571426	22
TAGCGCG	70	0.0	38.571426	1
GTACGGC	35	6.249824E-6	38.571426	9
CGTTTTT	2070	0.0	38.369568	1
TTGCGCG	95	0.0	37.894737	1
CAACGCG	30	1.1401214E-4	37.500004	1
ACCGCTA	30	1.1401214E-4	37.500004	26
>>END_MODULE