FastQCFastQC Report
Sat 18 Jun 2016
SRR3552396_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552396_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2294163
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC46500.2026883007005169RNA PCR Primer, Index 23 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGCT37220.1622378183241557Illumina Paired End PCR Primer 2 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC35790.1560046082165914RNA PCR Primer, Index 1 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26600.11594642577706989No Hit
TCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC24760.10792607151279138RNA PCR Primer, Index 23 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAACG253.8920258E-545.01
ACGATAG850.039.7058831
CGTTAGG2950.038.8983042
CCGTCGA301.1402766E-437.49999636
GCGATCG850.037.0588269
GCGTTAG1650.036.818181
CGTTTTT23150.036.25271
AGTTACG751.8189894E-1236.01
AAGGGCG10200.035.7352945
GTAATCG702.1827873E-1135.3571431
TAATGCG702.1827873E-1135.3571431
CGAAAGG3650.035.1369862
TCTAGCG458.7049557E-735.01
CGATCGA458.7049557E-735.010
CGTAAGG4000.034.8752
TTAGCGG5100.034.852942
TAAGGGA21050.034.631834
TATACGG1300.034.6153832
TACGCGG1700.034.4117662
ACGTAGG2750.034.3636362
Produced by FastQC (version 0.11.5)