##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552396_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2294163
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.234647232999574	31.0	31.0	34.0	30.0	34.0
2	31.42753806072193	31.0	31.0	34.0	28.0	34.0
3	31.50654596033499	31.0	31.0	34.0	28.0	34.0
4	35.21998349724932	37.0	35.0	37.0	33.0	37.0
5	35.03929537700678	37.0	35.0	37.0	32.0	37.0
6	35.144785701800615	37.0	35.0	37.0	32.0	37.0
7	35.61011227188303	37.0	35.0	37.0	35.0	37.0
8	35.65927050519078	37.0	35.0	37.0	35.0	37.0
9	37.142047012352656	39.0	37.0	39.0	34.0	39.0
10	36.521776787438384	38.0	35.0	39.0	32.0	39.0
11	36.2864024918892	38.0	35.0	39.0	32.0	39.0
12	36.309339397418576	38.0	35.0	39.0	32.0	39.0
13	36.08359301409708	38.0	35.0	39.0	31.0	39.0
14	37.27549437420096	39.0	36.0	40.0	32.0	41.0
15	37.358971441872264	39.0	36.0	40.0	32.0	41.0
16	37.3245266356401	39.0	36.0	40.0	32.0	41.0
17	37.33719530826711	39.0	36.0	40.0	32.0	41.0
18	37.295327315452305	39.0	36.0	40.0	32.0	41.0
19	37.26149449712161	39.0	36.0	40.0	32.0	41.0
20	36.883770682379584	39.0	35.0	40.0	31.0	41.0
21	36.986699724474676	39.0	35.0	40.0	31.0	41.0
22	37.086519135737085	39.0	35.0	40.0	31.0	41.0
23	36.91008746980925	39.0	35.0	40.0	31.0	41.0
24	36.97115549331063	39.0	35.0	40.0	31.0	41.0
25	36.954897712150355	39.0	35.0	40.0	31.0	41.0
26	36.920021375987666	39.0	35.0	40.0	31.0	41.0
27	36.7231138328009	39.0	35.0	40.0	31.0	41.0
28	36.67940377383822	39.0	35.0	40.0	30.0	41.0
29	36.74613704431638	39.0	35.0	40.0	30.0	41.0
30	36.61316349361401	39.0	35.0	40.0	30.0	41.0
31	36.48484697905075	38.0	35.0	40.0	30.0	41.0
32	36.24062370459292	38.0	35.0	40.0	30.0	41.0
33	36.16754868769133	38.0	35.0	40.0	30.0	41.0
34	36.08302940985449	38.0	35.0	40.0	29.0	41.0
35	35.927893092164766	38.0	35.0	40.0	29.0	41.0
36	35.99419090971304	38.0	35.0	40.0	29.0	41.0
37	36.000151689308915	38.0	35.0	40.0	29.0	41.0
38	35.97218854981098	38.0	35.0	40.0	29.0	41.0
39	35.933345189509204	38.0	35.0	40.0	29.0	41.0
40	35.837536391267754	38.0	35.0	40.0	29.0	41.0
41	35.751832803510474	38.0	34.0	40.0	28.0	41.0
42	35.76743675144268	38.0	34.0	40.0	28.0	41.0
43	35.6753103419417	38.0	34.0	40.0	28.0	41.0
44	35.58134884051395	38.0	34.0	40.0	28.0	41.0
45	35.49904039076561	38.0	34.0	40.0	28.0	41.0
46	35.37336492655491	38.0	34.0	40.0	27.0	41.0
47	35.27142840330003	38.0	34.0	40.0	27.0	41.0
48	35.20730741451239	38.0	34.0	40.0	27.0	41.0
49	35.12749486413999	37.0	34.0	40.0	27.0	41.0
50	34.98700092364841	37.0	34.0	40.0	26.0	41.0
51	33.69623082579572	36.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	16.0
9	68.0
10	83.0
11	60.0
12	41.0
13	23.0
14	44.0
15	86.0
16	217.0
17	391.0
18	886.0
19	1645.0
20	2782.0
21	4273.0
22	6472.0
23	9536.0
24	13586.0
25	17652.0
26	22583.0
27	28686.0
28	35799.0
29	45761.0
30	57311.0
31	73008.0
32	92537.0
33	119580.0
34	167186.0
35	195667.0
36	229391.0
37	305917.0
38	433997.0
39	428823.0
40	52.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.63691202412383	20.822365280932523	27.590062257999975	10.95066043694367
2	29.340984053879342	25.471686187947412	27.860138970073184	17.32719078810006
3	31.512538559814622	24.783025443266236	27.40677100973209	16.297664987187048
4	27.893397286940814	27.193752144028128	26.723602464166667	18.18924810486439
5	24.599036772888415	31.18919623409496	25.75802155295853	18.453745440058096
6	24.173696463590424	37.01567848492021	24.430958044393535	14.379667007095835
7	83.28139718058394	6.112076604844555	6.66940404844817	3.937122166123331
8	85.02560628865517	4.928158984344181	5.863140500478823	4.183094226521829
9	78.70003134040606	7.492667260347238	8.408251724049249	5.399049675197447
10	38.17156845437748	28.714393876982587	17.408963530490205	15.70507413814973
11	29.029497904028617	27.758271753140466	24.365967021523755	18.84626332130716
12	24.92612774244899	25.071365896843425	29.718245826473535	20.284260534234054
13	23.820452164907202	25.38520584631519	31.749749254957038	19.04459273382057
14	21.822642942110043	27.532176222875183	29.77621903936207	20.86896179565271
15	21.573053004516243	27.02541188224202	31.73540851282145	19.666126600420284
16	23.615976720049968	26.839287356652513	29.164797793356446	20.37993812994107
17	23.912381116773307	26.785803798596696	27.895620319916244	21.406194764713753
18	24.23729264224033	25.856009359404712	29.562328396020682	20.34436960233427
19	23.444105758832308	28.17729167456715	27.90486116287291	20.473741403727633
20	24.24906164034552	28.17925317425135	27.902550952133744	19.66913423326939
21	23.652983680758517	28.684186781845927	28.424658579185525	19.238170958210034
22	22.504285876809973	26.60129206163642	27.732946612773375	23.16147544878023
23	21.75991854109756	28.308450620117227	27.661373668741064	22.27025717004415
24	21.630067262003617	27.28201962981706	28.736275495681866	22.351637612497456
25	21.271635886377734	28.91372583377903	27.316367668731473	22.498270611111764
26	21.702381217027735	28.55952257969464	28.06125807102634	21.676838132251284
27	22.135829058353742	28.1326130706493	27.398576299940324	22.332981571056635
28	20.813255204621466	28.57578123263255	28.5005032336412	22.110460329104775
29	23.119281411129027	26.979164078576805	27.053875422103836	22.847679088190333
30	23.837451828836922	27.47995674239363	26.37214530964016	22.310446119129285
31	23.163698481755656	29.10813224692404	25.210850318830875	22.517318952489425
32	24.239254141924526	28.348378035911136	25.5268697123962	21.885498109768136
33	23.189982577523914	28.181083907289935	25.107501079914545	23.5214324352716
34	22.267641837131887	28.494182845769895	26.69897474590951	22.539200571188708
35	23.350258896163872	27.063334209469858	26.434041521897093	23.152365372469173
36	23.562362395348543	29.670254467533475	25.24249584706928	21.524887290048703
37	22.34213523624956	30.48323070331097	26.160695643683557	21.013938416755913
38	23.016542416558895	29.498906572898264	26.389842395679818	21.094708614863027
39	24.1411791577146	28.478403670532565	25.92945662535748	21.45096054639535
40	23.741861410893648	26.75228394843784	28.212249957827755	21.29360468284076
41	20.605815715797	28.378323597756566	27.38719960177198	23.628661084674455
42	21.874382944891014	27.88228212206369	27.57519844928194	22.66813648376336
43	21.981873127585093	26.912865389250896	28.267259126749057	22.838002356414954
44	22.85243027631428	26.999781619701825	26.75808126972669	23.389706834257197
45	23.39894767721387	25.89122917595655	26.886232582427667	23.82359056440192
46	23.347120496669156	27.13198669841681	26.923501076427435	22.5973917284866
47	20.952042204498984	27.54860923134058	29.094619693544004	22.40472887061643
48	21.39590778859218	26.988274154887858	28.58619897539974	23.029619081120217
49	22.874922139359757	26.55787753529283	28.15776385548891	22.409436469858505
50	21.7597005966882	25.470988765837475	28.35971114519762	24.409599492276705
51	21.664807600854864	25.197860832033296	26.84430007806769	26.293031489044154
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	513.0
1	746.5
2	980.0
3	3138.0
4	5296.0
5	4061.5
6	2827.0
7	2890.0
8	2953.0
9	3383.5
10	3814.0
11	4266.0
12	4718.0
13	5011.5
14	5305.0
15	5586.0
16	5867.0
17	6135.0
18	6403.0
19	6551.0
20	6699.0
21	7718.5
22	8738.0
23	9740.0
24	10742.0
25	14157.5
26	21151.0
27	24729.0
28	30027.0
29	35325.0
30	39856.0
31	44387.0
32	49444.5
33	54502.0
34	61336.5
35	68171.0
36	74566.0
37	80961.0
38	86313.0
39	91665.0
40	100019.0
41	108373.0
42	118529.0
43	128685.0
44	141125.5
45	153566.0
46	176866.0
47	200166.0
48	221195.5
49	242225.0
50	235806.0
51	229387.0
52	203409.0
53	177431.0
54	155504.0
55	133577.0
56	120020.5
57	106464.0
58	96586.0
59	86708.0
60	79180.0
61	71652.0
62	63037.5
63	54423.0
64	46560.0
65	38697.0
66	32530.5
67	26364.0
68	22324.0
69	18284.0
70	15570.0
71	12856.0
72	10953.0
73	9050.0
74	7378.5
75	4764.5
76	3822.0
77	3030.0
78	2238.0
79	1760.5
80	1283.0
81	906.0
82	529.0
83	424.5
84	320.0
85	202.5
86	85.0
87	75.0
88	65.0
89	44.0
90	23.0
91	16.0
92	9.0
93	7.5
94	6.0
95	3.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2294163.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.08490181627075
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.27566722865507	23.581484521492392
2	11.436609057678776	7.567581755687501
3	4.448356716844793	4.415203356616755
4	2.2075777007828266	2.9214996592875417
5	1.3982810705486362	2.313099596532579
6	0.9900848353628892	1.9654115740655866
7	0.7545643390221186	1.7475280949424223
8	0.6125504367097266	1.621293684484406
9	0.5383980456309915	1.6031561829996075
>10	6.01392590881663	42.421210369559894
>50	0.2794626063404258	5.859472431261018
>100	0.04144770080340636	2.460920988168084
>500	0.0017376776716827836	0.4064606287525725
>1k	0.0013366751320636788	1.1156771561494825
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	4650	0.2026883007005169	RNA PCR Primer, Index 23 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGCT	3722	0.1622378183241557	Illumina Paired End PCR Primer 2 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	3579	0.1560046082165914	RNA PCR Primer, Index 1 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2660	0.11594642577706989	No Hit
TCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	2476	0.10792607151279138	RNA PCR Primer, Index 23 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.717776374215781E-5	0.0	0.0	0.19009111383977512	0.0
2	8.717776374215781E-5	0.0	0.0	0.8072660922523813	0.0
3	8.717776374215781E-5	0.0	0.0	1.1066345329429512	0.0
4	8.717776374215781E-5	0.0	0.0	1.4691632634647145	0.0
5	8.717776374215781E-5	0.0	0.0	2.3688813741656545	0.0
6	8.717776374215781E-5	0.0	0.0	3.1192203866944066	0.0
7	8.717776374215781E-5	0.0	0.0	3.8471983028232954	0.0
8	8.717776374215781E-5	0.0	0.0	5.2862416489150945	0.0
9	8.717776374215781E-5	0.0	0.0	5.868153221893998	0.0
10	8.717776374215781E-5	0.0	0.0	7.261341064257422	0.0
11	8.717776374215781E-5	0.0	0.0	8.967191956282095	0.0
12	8.717776374215781E-5	0.0	0.0	10.34590828986432	0.0
13	8.717776374215781E-5	0.0	0.0	10.940068338648997	0.0
14	8.717776374215781E-5	0.0	0.0	11.217250038467188	0.0
15	8.717776374215781E-5	0.0	0.0	11.600483487877714	0.0
16	8.717776374215781E-5	0.0	0.0	12.406267558146478	0.0
17	8.717776374215781E-5	0.0	0.0	13.402317097782502	4.3588881871078905E-5
18	8.717776374215781E-5	0.0	0.0	14.482100879492869	4.3588881871078905E-5
19	8.717776374215781E-5	0.0	0.0	15.14312627306778	4.3588881871078905E-5
20	8.717776374215781E-5	0.0	0.0	15.791205768726982	4.3588881871078905E-5
21	1.3076664561323671E-4	0.0	0.0	16.576938953335052	4.3588881871078905E-5
22	1.3076664561323671E-4	0.0	0.0	17.44278850282216	4.3588881871078905E-5
23	1.3076664561323671E-4	0.0	0.0	18.305891952751395	4.3588881871078905E-5
24	1.3076664561323671E-4	0.0	0.0	18.989060498316814	4.3588881871078905E-5
25	1.7435552748431562E-4	0.0	0.0	19.620183918928166	4.3588881871078905E-5
26	1.7435552748431562E-4	0.0	0.0	20.196559703909443	4.3588881871078905E-5
27	1.7435552748431562E-4	0.0	0.0	20.787276231026304	4.3588881871078905E-5
28	1.7435552748431562E-4	0.0	0.0	21.407022953469305	4.3588881871078905E-5
29	1.7435552748431562E-4	0.0	0.0	22.038059196316915	4.3588881871078905E-5
30	1.7435552748431562E-4	0.0	0.0	22.77606255527615	4.3588881871078905E-5
31	1.7435552748431562E-4	0.0	0.0	23.43979045952707	4.3588881871078905E-5
32	1.7435552748431562E-4	0.0	0.0	24.089787865988598	4.3588881871078905E-5
33	1.7435552748431562E-4	0.0	0.0	24.72993418514726	4.3588881871078905E-5
34	1.7435552748431562E-4	0.0	0.0	25.370342037597155	4.3588881871078905E-5
35	1.7435552748431562E-4	0.0	0.0	26.025570109883212	4.3588881871078905E-5
36	1.7435552748431562E-4	0.0	0.0	26.67430343877048	4.3588881871078905E-5
37	2.6153329122647343E-4	0.0	0.0	27.306080692609896	4.3588881871078905E-5
38	2.6153329122647343E-4	0.0	0.0	27.96104723160473	4.3588881871078905E-5
39	0.0017871441567142352	0.0	0.0	28.628044301995978	4.3588881871078905E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGAACG	25	3.8920258E-5	45.0	1
ACGATAG	85	0.0	39.705883	1
CGTTAGG	295	0.0	38.898304	2
CCGTCGA	30	1.1402766E-4	37.499996	36
GCGATCG	85	0.0	37.058826	9
GCGTTAG	165	0.0	36.81818	1
CGTTTTT	2315	0.0	36.2527	1
AGTTACG	75	1.8189894E-12	36.0	1
AAGGGCG	1020	0.0	35.735294	5
GTAATCG	70	2.1827873E-11	35.357143	1
TAATGCG	70	2.1827873E-11	35.357143	1
CGAAAGG	365	0.0	35.136986	2
TCTAGCG	45	8.7049557E-7	35.0	1
CGATCGA	45	8.7049557E-7	35.0	10
CGTAAGG	400	0.0	34.875	2
TTAGCGG	510	0.0	34.85294	2
TAAGGGA	2105	0.0	34.63183	4
TATACGG	130	0.0	34.615383	2
TACGCGG	170	0.0	34.411766	2
ACGTAGG	275	0.0	34.363636	2
>>END_MODULE