FastQCFastQC Report
Sat 18 Jun 2016
SRR3552395_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552395_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2377775
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC38400.16149551576578944Illumina PCR Primer Index 8 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGCT29370.12351883588649051RNA PCR Primer, Index 40 (96% over 26bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25310.10644404958417006No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATTCG207.0348085E-445.00000424
CGAATAT2300.038.15217614
AATTGCG659.094947E-1238.076921
CTACGAA2400.036.562511
CGTTAGG3700.036.4864852
CCCTCGT2100.036.4285714
TAACGCG504.8827133E-836.01
TAGGGTA10300.035.825245
TACGAAT2450.035.81632612
GGCCGAT3400.035.7352948
TTACGAG1200.035.6251
CGACCAA4600.035.21739229
ACACGAC4550.035.1098926
CGTAAGG3550.034.8591542
GACCGAT10400.034.6153839
TACGGGA8450.034.6153834
CACGACC4750.034.5789527
TACACGC14150.034.3462935
TTACACG14100.034.3085134
TAGGGAT17150.034.110795
Produced by FastQC (version 0.11.5)