##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552395_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2377775
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.367302625353535	31.0	31.0	34.0	30.0	34.0
2	31.560230888120195	31.0	31.0	34.0	30.0	34.0
3	31.64587061433483	31.0	31.0	34.0	30.0	34.0
4	35.344462365026125	37.0	35.0	37.0	33.0	37.0
5	35.15326134726793	37.0	35.0	37.0	33.0	37.0
6	35.254140530538	37.0	35.0	37.0	32.0	37.0
7	35.672546393161674	37.0	35.0	37.0	35.0	37.0
8	35.70435049573656	37.0	35.0	37.0	35.0	37.0
9	37.17205791128261	39.0	37.0	39.0	34.0	39.0
10	36.61436637192333	38.0	35.0	39.0	32.0	39.0
11	36.3687514588218	38.0	35.0	39.0	32.0	39.0
12	36.342796521958554	38.0	35.0	39.0	32.0	39.0
13	36.10883409910526	38.0	35.0	39.0	31.0	39.0
14	37.31620569650198	39.0	36.0	41.0	32.0	41.0
15	37.41747684284678	39.0	36.0	41.0	32.0	41.0
16	37.39930060665959	39.0	36.0	41.0	32.0	41.0
17	37.41729516039154	39.0	36.0	41.0	32.0	41.0
18	37.37612053285109	39.0	36.0	41.0	32.0	41.0
19	37.34274395180368	39.0	36.0	41.0	32.0	41.0
20	36.95551050877396	39.0	35.0	40.0	31.0	41.0
21	37.05315221162642	39.0	35.0	40.0	31.0	41.0
22	37.154246722250846	39.0	35.0	40.0	31.0	41.0
23	36.978933666978584	39.0	35.0	40.0	31.0	41.0
24	37.03537761142244	39.0	35.0	40.0	31.0	41.0
25	37.042304255028334	39.0	35.0	40.0	31.0	41.0
26	36.995156396210746	39.0	35.0	40.0	31.0	41.0
27	36.772792631767096	39.0	35.0	40.0	31.0	41.0
28	36.74512012280388	39.0	35.0	40.0	31.0	41.0
29	36.79649251926696	39.0	35.0	40.0	31.0	41.0
30	36.678091913658776	39.0	35.0	40.0	30.0	41.0
31	36.59196854201932	39.0	35.0	40.0	30.0	41.0
32	36.323025096991934	38.0	35.0	40.0	30.0	41.0
33	36.242487199167286	38.0	35.0	40.0	30.0	41.0
34	36.18024035074807	38.0	35.0	40.0	30.0	41.0
35	36.00674412002818	38.0	35.0	40.0	29.0	41.0
36	36.06976017495347	38.0	35.0	40.0	29.0	41.0
37	36.09311183774747	38.0	35.0	40.0	30.0	41.0
38	36.04995510508774	38.0	35.0	40.0	29.0	41.0
39	36.00267687228607	38.0	35.0	40.0	29.0	41.0
40	35.88266761993881	38.0	35.0	40.0	29.0	41.0
41	35.828136388009796	38.0	35.0	40.0	29.0	41.0
42	35.82107348256248	38.0	35.0	40.0	29.0	41.0
43	35.73441011029219	38.0	34.0	40.0	29.0	41.0
44	35.63108410173376	38.0	34.0	40.0	28.0	41.0
45	35.53084879772056	38.0	34.0	40.0	28.0	41.0
46	35.37979539695724	38.0	34.0	40.0	27.0	41.0
47	35.30625101197548	38.0	34.0	40.0	27.0	41.0
48	35.24684926033792	38.0	34.0	40.0	27.0	41.0
49	35.1647178559788	37.0	34.0	40.0	27.0	41.0
50	35.04097065533955	37.0	34.0	40.0	27.0	41.0
51	33.73434113824899	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	28.0
9	72.0
10	105.0
11	58.0
12	28.0
13	44.0
14	47.0
15	107.0
16	225.0
17	488.0
18	936.0
19	1864.0
20	2893.0
21	4532.0
22	6723.0
23	9771.0
24	13854.0
25	17759.0
26	22607.0
27	28345.0
28	35283.0
29	44732.0
30	56452.0
31	73036.0
32	92631.0
33	122005.0
34	176654.0
35	205107.0
36	231991.0
37	311301.0
38	447609.0
39	470418.0
40	69.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.44395495789131	20.937094552680552	26.626867554751815	10.992082934676326
2	29.272702421381336	25.31538938713713	27.836191397419856	17.575716794061673
3	32.0426449096319	24.463836990463776	27.169685945894795	16.323832154009526
4	28.299944275635834	27.200723365331037	26.03942761615376	18.459904742879374
5	25.063052643753085	31.26553185225684	25.247763139910212	18.423652364079864
6	24.600813785997413	37.29524450379031	23.68874262703578	14.4151990831765
7	84.02956545509983	6.082114581909559	6.069035127377485	3.819284835613126
8	85.44904374888289	5.257688385149983	5.481342852036041	3.811925013931091
9	78.89510151296906	8.011733658567358	7.874798919157616	5.218365909305969
10	39.83463532083565	27.17061118062054	16.99458527404822	16.00016822449559
11	31.15669060361052	27.55601350001577	22.602180610024078	18.68511528634963
12	26.70336764412108	25.14161348319332	28.249981600445796	19.905037272239802
13	24.83485611548612	25.371576368663984	29.996908874893546	19.796658640956355
14	22.013689268328584	27.596219154461625	28.82846358465372	21.561627992556065
15	21.698815068709195	26.85977436889529	31.826097927684494	19.61531263471102
16	24.072883262714093	26.08577346468863	29.66924961360936	20.17209365898792
17	23.903901756894577	25.613146744330308	28.24855169223329	22.23439980654183
18	24.469682791685504	25.425029702137504	29.055314316955975	21.049973189221017
19	24.956945043160097	27.25236828547697	27.206905615543942	20.58378105581899
20	25.733006697437734	26.993765179632216	27.314443124349445	19.958784998580605
21	24.60468294939597	28.512159476821818	27.16346163955799	19.71969593422422
22	23.55698079086541	26.544311383541334	26.588260033014055	23.310447792579197
23	22.88568094121605	27.704471617373382	27.169223328531928	22.24062411287864
24	22.393708403864956	26.59263386989938	28.29451903565308	22.719138690582582
25	22.271661532314873	28.205317996866818	26.841227618256564	22.681792852561745
26	21.816908664612928	28.644299818107267	27.344597365183837	22.19419415209597
27	22.377180347173304	28.348939659976235	27.350527278653363	21.923352714197094
28	20.640472710832604	29.07440779720537	28.042308460640726	22.2428110313213
29	22.514619760069813	27.80528014635531	26.78449390711905	22.895606186455826
30	22.751185456992356	28.197621726193606	26.29832511486579	22.75286770194825
31	23.173975670532325	28.924477715511347	24.70099147312088	23.200555140835444
32	23.43501803156312	29.24427248162673	25.27737065113394	22.04333883567621
33	22.959195045788604	28.41109861109651	24.88540757641072	23.744298766704166
34	21.50068866902882	28.887888887720663	26.682297526048508	22.929124917202007
35	21.995563078928832	27.540620958669344	26.563867481153597	23.899948481248227
36	21.457581142034044	30.803082713881675	25.020954463731847	22.718381680352433
37	22.278769017253524	29.219375256279505	25.527814868942606	22.974040857524365
38	22.41629254239783	29.30609498375582	25.938661143295725	22.338951330550618
39	22.802620096518805	27.34375624270589	25.77767030101671	24.075953359758596
40	22.31005877343315	26.834708919052474	28.055976700907358	22.799255606607016
41	19.62847620149089	26.98270441904722	27.230120595935276	26.158698783526617
42	21.15654340717688	27.254134642680654	26.951582887363184	24.63773906277928
43	21.77809086225568	25.9169601833647	27.81259791191345	24.492351042466172
44	21.83440401215422	27.476401257478106	26.0735351326345	24.615659597733174
45	22.498301983997646	26.478914110880968	26.206306315778406	24.816477589342977
46	21.370735246186033	28.08394402329909	26.51087676504295	24.034443965471922
47	20.498617404926875	27.42256941888951	28.1091356415136	23.969677534670016
48	20.791075690508983	26.613451651228566	27.957397146491992	24.638075511770456
49	21.77653478567148	26.327974682213412	27.341821661006616	24.553668871108496
50	20.426533208566834	26.219301658062683	27.826055871560595	25.528109261809885
51	20.685893324641736	25.666137460440957	26.272754991536207	27.3752142233811
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	549.0
1	923.0
2	1297.0
3	3194.0
4	5091.0
5	4007.5
6	2924.0
7	3056.0
8	3188.0
9	3485.5
10	3783.0
11	4103.5
12	4424.0
13	5012.5
14	5601.0
15	5717.5
16	5834.0
17	6054.5
18	6275.0
19	6576.5
20	6878.0
21	8016.0
22	9154.0
23	10211.0
24	11268.0
25	14267.0
26	21399.5
27	25533.0
28	30580.5
29	35628.0
30	40010.5
31	44393.0
32	50557.0
33	56721.0
34	62674.0
35	68627.0
36	74223.5
37	79820.0
38	87177.0
39	94534.0
40	104540.5
41	114547.0
42	123448.5
43	132350.0
44	145135.5
45	157921.0
46	174842.0
47	191763.0
48	208404.5
49	225046.0
50	220540.5
51	216035.0
52	195579.5
53	175124.0
54	155339.0
55	135554.0
56	123857.0
57	112160.0
58	106005.5
59	99851.0
60	94317.5
61	88784.0
62	81372.0
63	73960.0
64	62605.5
65	51251.0
66	43638.5
67	36026.0
68	31445.5
69	26865.0
70	22402.5
71	17940.0
72	15583.5
73	13227.0
74	11005.5
75	7065.5
76	5347.0
77	4274.0
78	3201.0
79	2456.5
80	1712.0
81	1183.0
82	654.0
83	581.5
84	509.0
85	364.5
86	220.0
87	157.5
88	95.0
89	68.5
90	42.0
91	27.5
92	13.0
93	9.5
94	6.0
95	3.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2377775.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.267523007582895
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.33526016062758	20.164711078424517
2	11.368195348549069	6.427014471396155
3	4.358444405337968	3.696072825154859
4	2.184597790393581	2.470126732090612
5	1.340576432077592	1.8947387568588363
6	0.8633596825326316	1.464302381388638
7	0.6746754910685785	1.3349983476503333
8	0.539251375414227	1.2194640529113907
9	0.4401904072873183	1.11987832191404
>10	5.97040252186177	40.128577491252
>50	0.8506901196331388	15.587005597270853
>100	0.0710183310526278	3.1720523354885635
>500	0.0015172428017104919	0.29593200105754197
>1k	0.0018206913620525903	1.0251256071416475
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC	3840	0.16149551576578944	Illumina PCR Primer Index 8 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGCT	2937	0.12351883588649051	RNA PCR Primer, Index 40 (96% over 26bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2531	0.10644404958417006	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.2056123897341E-5	0.0	0.0	0.1469861530212068	0.0
2	4.2056123897341E-5	0.0	0.0	0.5997623828999801	0.0
3	4.2056123897341E-5	0.0	0.0	0.8123981453249361	0.0
4	4.2056123897341E-5	0.0	0.0	1.0677208735056933	0.0
5	4.2056123897341E-5	0.0	0.0	1.7445300753856021	0.0
6	4.2056123897341E-5	0.0	0.0	2.314727003185751	0.0
7	4.2056123897341E-5	0.0	0.0	2.8603631546298534	0.0
8	4.2056123897341E-5	0.0	0.0	3.928967206737391	0.0
9	4.2056123897341E-5	0.0	0.0	4.373878941447361	0.0
10	4.2056123897341E-5	0.0	0.0	5.495389597417754	0.0
11	4.2056123897341E-5	0.0	0.0	6.905994049058468	0.0
12	4.2056123897341E-5	0.0	0.0	8.039532756463501	0.0
13	4.2056123897341E-5	0.0	0.0	8.51022489512254	0.0
14	4.2056123897341E-5	0.0	0.0	8.72820178528246	0.0
15	4.2056123897341E-5	0.0	0.0	9.043917107379798	0.0
16	4.2056123897341E-5	0.0	0.0	9.737002029207979	0.0
17	4.2056123897341E-5	0.0	0.0	10.545699235629948	0.0
18	4.2056123897341E-5	0.0	0.0	11.427784378252777	0.0
19	4.2056123897341E-5	0.0	0.0	11.942593390880129	0.0
20	4.2056123897341E-5	0.0	0.0	12.459967827065219	0.0
21	4.2056123897341E-5	0.0	0.0	13.089085384445543	0.0
22	4.2056123897341E-5	0.0	0.0	13.767576831281344	0.0
23	4.2056123897341E-5	0.0	0.0	14.472016906561807	0.0
24	4.2056123897341E-5	0.0	0.0	15.03312971160013	0.0
25	8.4112247794682E-5	0.0	0.0	15.556560229626436	0.0
26	8.4112247794682E-5	0.0	0.0	16.026957975418195	0.0
27	8.4112247794682E-5	0.0	0.0	16.506650124591268	0.0
28	8.4112247794682E-5	0.0	0.0	16.99424882505704	0.0
29	8.4112247794682E-5	0.0	0.0	17.516165322623042	0.0
30	8.4112247794682E-5	0.0	0.0	18.109703399186213	0.0
31	8.4112247794682E-5	0.0	0.0	18.670690035852846	0.0
32	8.4112247794682E-5	0.0	0.0	19.209513095225578	0.0
33	8.4112247794682E-5	0.0	0.0	19.72764454164082	0.0
34	8.4112247794682E-5	0.0	0.0	20.262640493738896	0.0
35	8.4112247794682E-5	0.0	0.0	20.838809391132465	0.0
36	8.4112247794682E-5	0.0	0.0	21.3791464709655	0.0
37	8.4112247794682E-5	0.0	0.0	21.933614408428046	0.0
38	8.4112247794682E-5	0.0	0.0	22.484044957996446	0.0
39	8.4112247794682E-5	0.0	0.0	23.072452187444146	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAATTCG	20	7.0348085E-4	45.000004	24
CGAATAT	230	0.0	38.152176	14
AATTGCG	65	9.094947E-12	38.07692	1
CTACGAA	240	0.0	36.5625	11
CGTTAGG	370	0.0	36.486485	2
CCCTCGT	210	0.0	36.42857	14
TAACGCG	50	4.8827133E-8	36.0	1
TAGGGTA	1030	0.0	35.82524	5
TACGAAT	245	0.0	35.816326	12
GGCCGAT	340	0.0	35.735294	8
TTACGAG	120	0.0	35.625	1
CGACCAA	460	0.0	35.217392	29
ACACGAC	455	0.0	35.10989	26
CGTAAGG	355	0.0	34.859154	2
GACCGAT	1040	0.0	34.615383	9
TACGGGA	845	0.0	34.615383	4
CACGACC	475	0.0	34.57895	27
TACACGC	1415	0.0	34.34629	35
TTACACG	1410	0.0	34.30851	34
TAGGGAT	1715	0.0	34.11079	5
>>END_MODULE