FastQCFastQC Report
Sat 18 Jun 2016
SRR3552394_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552394_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2453268
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC63550.25904222449402187TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGCT62280.2538654561996488Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC42360.17266764169263205TruSeq Adapter, Index 13 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC37420.15253123588617304TruSeq Adapter, Index 13 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAC207.034853E-445.00000418
CGACGGT453.8562575E-1045.00000430
TTCCGCG451.9297659E-840.0000041
TTTCGCG750.039.01
TAGTCCG356.251188E-638.5714261
TTTAGCG1950.038.0769231
CGAACCG301.1402993E-437.50000422
TCCGCGG900.037.5000042
ATCGTAG301.1402993E-437.5000041
ACGACAC601.5643309E-1037.50000440
CGTAAGG2850.037.1052632
CGTTTTT26450.037.0888441
TTAGTCG552.752131E-936.818181
CTCGCGG800.036.5625042
GCGTTAG1550.036.2903251
ATTCGCG751.8189894E-1236.01
CGTTTCG250.00210757436.042
TACGAAT1200.035.62500412
TACGCGG2150.035.5813942
TAACGTC4500.035.532
Produced by FastQC (version 0.11.5)