##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552394_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2453268
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.182324556469165	31.0	31.0	34.0	28.0	34.0
2	31.378650029267085	31.0	31.0	34.0	28.0	34.0
3	31.45859400603603	31.0	31.0	34.0	28.0	34.0
4	35.18023917484759	37.0	35.0	37.0	32.0	37.0
5	34.99679407223344	37.0	35.0	37.0	32.0	37.0
6	35.10321497692058	37.0	35.0	37.0	32.0	37.0
7	35.608993391671845	37.0	35.0	37.0	35.0	37.0
8	35.675337957369514	37.0	35.0	37.0	35.0	37.0
9	37.123959143477194	39.0	37.0	39.0	34.0	39.0
10	36.459950971520435	38.0	35.0	39.0	32.0	39.0
11	36.235447574419105	38.0	35.0	39.0	31.0	39.0
12	36.30619606174295	38.0	35.0	39.0	32.0	39.0
13	36.092690240120525	38.0	35.0	39.0	31.0	39.0
14	37.23431520730715	39.0	36.0	40.0	32.0	41.0
15	37.33463486255884	39.0	36.0	40.0	32.0	41.0
16	37.304574958789665	39.0	36.0	40.0	32.0	41.0
17	37.32807748684612	39.0	36.0	40.0	32.0	41.0
18	37.289031202461366	39.0	36.0	40.0	32.0	41.0
19	37.26253307832654	39.0	36.0	40.0	32.0	41.0
20	36.8956689607495	39.0	35.0	40.0	31.0	41.0
21	36.9893407487482	39.0	35.0	40.0	31.0	41.0
22	37.08516599083345	39.0	35.0	40.0	31.0	41.0
23	36.90978808674796	39.0	35.0	40.0	31.0	41.0
24	36.978799299546566	39.0	35.0	40.0	31.0	41.0
25	36.98926860008772	39.0	35.0	40.0	31.0	41.0
26	36.963427558668684	39.0	35.0	40.0	31.0	41.0
27	36.758392071310595	39.0	35.0	40.0	31.0	41.0
28	36.73516590930954	39.0	35.0	40.0	30.0	41.0
29	36.81134348142967	39.0	35.0	40.0	31.0	41.0
30	36.6757973446032	39.0	35.0	40.0	30.0	41.0
31	36.59354338784022	39.0	35.0	40.0	30.0	41.0
32	36.34665800882741	39.0	35.0	40.0	30.0	41.0
33	36.28139078160233	38.0	35.0	40.0	30.0	41.0
34	36.25219829223713	38.0	35.0	40.0	30.0	41.0
35	36.1128091182863	38.0	35.0	40.0	29.0	41.0
36	36.17550467376577	38.0	35.0	40.0	30.0	41.0
37	36.18987448578794	38.0	35.0	40.0	30.0	41.0
38	36.15422530273904	38.0	35.0	40.0	30.0	41.0
39	36.12514980018489	38.0	35.0	40.0	30.0	41.0
40	36.02425132517116	38.0	35.0	40.0	29.0	41.0
41	35.94620970884551	38.0	35.0	40.0	29.0	41.0
42	35.94073048684449	38.0	35.0	40.0	29.0	41.0
43	35.84857015213992	38.0	35.0	40.0	29.0	41.0
44	35.766257090542084	38.0	35.0	40.0	28.0	41.0
45	35.68607547157506	38.0	34.0	40.0	28.0	41.0
46	35.5548443953127	38.0	34.0	40.0	28.0	41.0
47	35.46425624921533	38.0	34.0	40.0	27.0	41.0
48	35.4048685263901	38.0	34.0	40.0	27.0	41.0
49	35.33065405002633	38.0	34.0	40.0	27.0	41.0
50	35.2102342671082	38.0	34.0	40.0	27.0	41.0
51	33.890308763657295	37.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	12.0
9	56.0
10	94.0
11	65.0
12	34.0
13	31.0
14	46.0
15	95.0
16	193.0
17	478.0
18	976.0
19	1712.0
20	2890.0
21	4602.0
22	6875.0
23	9977.0
24	14137.0
25	18427.0
26	23530.0
27	29402.0
28	36970.0
29	46722.0
30	60723.0
31	76739.0
32	97409.0
33	126612.0
34	172350.0
35	204070.0
36	242010.0
37	323834.0
38	474531.0
39	477613.0
40	50.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.88394256151387	21.6706042715268	27.764312745285064	10.681140421674273
2	28.926558370304427	25.266460900317455	28.470513616938714	17.336467112439408
3	30.077390647903123	25.217301982498448	28.465785230150153	16.23952213944828
4	26.876843459418215	27.78881068028442	27.317194860080512	18.017151000216852
5	23.99089704019292	31.206129945851817	26.262193938860328	18.540779075094935
6	24.2904566480303	36.04746811192255	24.920840283246672	14.741234956800481
7	83.21895528739624	5.696116363968389	6.780873512392449	4.304054836242922
8	85.00628549347238	4.3930381841690345	6.11033935142838	4.490336970930204
9	78.33245287510374	7.807585636791414	8.76699977336353	5.092961714741316
10	35.61213043173432	33.985728424289555	16.380273170318123	14.021867973657995
11	26.132815493456075	26.915893412378917	28.418460600309466	18.532830493855542
12	23.29109579548586	24.550436397491023	31.901202803770317	20.257265003252805
13	22.936629834164062	25.5069564352529	33.537836061938606	18.018577668644436
14	20.716489189114277	28.366570631500515	30.79565705825862	20.121283121126595
15	19.705388893508577	27.829939492953887	33.381187868589976	19.083483744947554
16	22.69805011111709	28.024903924071893	29.809054697652275	19.467991267158745
17	22.885677390321806	27.21182520621473	28.986967587723804	20.91552981573966
18	23.052923691989623	26.020720117003115	31.08021626662884	19.84613992437842
19	21.610032006287124	29.0057996109679	29.288116911809066	20.09605147093591
20	22.34729348770701	29.2485370534324	29.174024199557486	19.2301452593031
21	22.31582525839003	29.28836148353951	29.70417418724738	18.691639070823083
22	21.394849645452517	27.01372210455604	28.72368611990211	22.867742130089333
23	19.76767316086135	29.105136495482757	29.002579416517072	22.12461092713882
24	20.111907871459618	27.723713838031554	29.892086800137612	22.27229149037121
25	19.388750026495273	29.730914029775796	28.23841504474847	22.641920898980462
26	19.55636318575875	29.2315800801217	29.064415302364033	22.14764143175552
27	20.67144722875772	28.160355900782143	29.27617365897244	21.892023211487697
28	18.277253035542795	28.90678882209363	30.232449125003875	22.583509017359702
29	20.048482269364783	27.00614038091232	29.033313930642716	23.91206341908018
30	21.045968071975828	27.9708943335991	28.892481375862726	22.090656218562344
31	20.800336530701088	29.03033830792233	27.06854693413031	23.10077822724627
32	20.96118320542232	28.402074294369793	27.37740026772452	23.259342232483366
33	20.222821151215438	28.450010353536587	27.89144928316026	23.43571921208771
34	18.835814105919123	28.251132774731502	28.354505092798664	24.55854802655071
35	19.470151650777655	26.79319992760677	29.09205190790407	24.644596513711505
36	19.790499855702677	29.257300873773268	28.01622162764117	22.935977642882882
37	19.457026301243893	28.639634968539923	29.025039253762735	22.87829947645345
38	19.78397794289087	29.765113310082715	28.271962133774213	22.1789466132522
39	19.987665432394667	27.649200984156643	27.999427702150765	24.363705881297925
40	21.83251075707994	26.257465552071768	28.198305280955854	23.711718409892438
41	20.281518366521716	25.896559201848312	27.104172882864813	26.717749548765156
42	20.866819279426462	25.756867981810384	28.330007157799308	25.04630558096384
43	20.098945569746153	26.01542106284352	29.907535581110583	23.97809778629974
44	19.618484405291227	27.253687734075527	28.719161542888916	24.40866631774433
45	20.129109416500764	26.047296911711236	28.964874608073803	24.858719063714197
46	20.953519957868444	26.842644179111293	28.331230016451524	23.87260584656874
47	18.812579791527057	26.5748381342764	30.11505469439132	24.497527379805224
48	19.729153113316606	26.148345798339196	29.564360681344233	24.55814040699997
49	21.117668350950648	24.871885175203033	29.73250374602367	24.277942727822644
50	19.47027393664288	24.782616493591405	29.801432212053474	25.945677357712242
51	19.344441781330048	24.467118961320168	27.9284203764122	28.260018880937594
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	774.0
1	870.0
2	966.0
3	2898.5
4	4831.0
5	3828.0
6	2825.0
7	3018.5
8	3212.0
9	3716.0
10	4220.0
11	4763.0
12	5306.0
13	5747.0
14	6188.0
15	6568.0
16	6948.0
17	7299.5
18	7651.0
19	7872.5
20	8094.0
21	9072.5
22	10051.0
23	11671.5
24	13292.0
25	15897.5
26	22568.0
27	26633.0
28	31230.5
29	35828.0
30	41923.5
31	48019.0
32	55584.0
33	63149.0
34	70897.5
35	78646.0
36	86507.0
37	94368.0
38	102992.5
39	111617.0
40	122452.5
41	133288.0
42	148480.5
43	163673.0
44	179741.0
45	195809.0
46	220261.0
47	244713.0
48	266264.0
49	287815.0
50	272716.0
51	257617.0
52	219603.0
53	181589.0
54	153870.0
55	126151.0
56	107422.0
57	88693.0
58	77962.5
59	67232.0
60	59465.5
61	51699.0
62	44195.5
63	36692.0
64	31002.5
65	25313.0
66	20244.0
67	15175.0
68	12529.0
69	9883.0
70	8115.0
71	6347.0
72	5185.0
73	4023.0
74	3378.0
75	2124.0
76	1515.0
77	1264.5
78	1014.0
79	821.0
80	628.0
81	461.0
82	294.0
83	217.5
84	141.0
85	99.5
86	58.0
87	41.0
88	24.0
89	21.0
90	18.0
91	10.5
92	3.0
93	4.0
94	5.0
95	3.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2453268.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.7958152373335
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.8892726686578	24.29556576656248
2	11.552390491664495	7.808449092116431
3	4.434982177479687	4.496515147529115
4	2.2624163053522106	3.0584081378245602
5	1.36138097334232	2.3004489921349145
6	0.9575435961635842	1.9416579874581827
7	0.7481550989684291	1.7699158045524195
8	0.5600546544123344	1.5142002898662335
9	0.49023017578934	1.491095560026785
>10	5.350638229938172	39.59659371135553
>50	0.3490571909119197	7.408552719390829
>100	0.040448076836657805	2.478841472061703
>500	0.0020827188647419485	0.4922548271374918
>1k	0.001102615869569266	0.8265111329227526
>5k	2.450257487931702E-4	0.5209893590605649
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	6355	0.25904222449402187	TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGCT	6228	0.2538654561996488	Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	4236	0.17266764169263205	TruSeq Adapter, Index 13 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	3742	0.15253123588617304	TruSeq Adapter, Index 13 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2993557980620136	0.0
2	0.0	0.0	0.0	1.0931133492141911	0.0
3	0.0	0.0	0.0	1.5114532941366374	0.0
4	0.0	0.0	0.0	1.9921182683669294	0.0
5	0.0	0.0	0.0	3.152570367363044	0.0
6	0.0	0.0	0.0	4.077336842122426	0.0
7	0.0	0.0	0.0	4.998108645284575	0.0
8	0.0	0.0	0.0	6.727597637110988	0.0
9	0.0	0.0	0.0	7.462739496867036	0.0
10	0.0	0.0	0.0	9.090935030335046	0.0
11	0.0	0.0	0.0	11.014817785908429	0.0
12	0.0	0.0	0.0	12.597278405783632	0.0
13	0.0	0.0	0.0	13.283995062912002	0.0
14	0.0	0.0	0.0	13.619139857528815	0.0
15	0.0	0.0	0.0	14.05215410627783	0.0
16	0.0	0.0	0.0	14.904364301005842	0.0
17	0.0	0.0	0.0	16.018836914678705	0.0
18	0.0	0.0	0.0	17.258326444562925	0.0
19	0.0	0.0	0.0	18.007531178819438	0.0
20	0.0	0.0	0.0	18.74528180370021	0.0
21	0.0	0.0	0.0	19.588687416132277	0.0
22	0.0	0.0	0.0	20.497842062098393	0.0
23	0.0	0.0	0.0	21.427703781242002	0.0
24	0.0	0.0	0.0	22.140181993977013	0.0
25	8.15239101476072E-5	0.0	0.0	22.784017074367743	0.0
26	1.222858652214108E-4	0.0	0.0	23.37596218594952	0.0
27	1.222858652214108E-4	0.0	0.0	23.994810187880002	0.0
28	1.222858652214108E-4	0.0	0.0	24.63326469020099	0.0
29	1.630478202952144E-4	0.0	0.0	25.290918073361738	0.0
30	1.630478202952144E-4	0.0	0.0	26.072161704306257	0.0
31	1.630478202952144E-4	0.0	0.0	26.76409589168407	0.0
32	1.630478202952144E-4	0.0	0.0	27.419058985809947	0.0
33	1.630478202952144E-4	0.0	0.0	28.05722815444542	0.0
34	2.03809775369018E-4	0.0	0.0	28.694622845934486	0.0
35	2.03809775369018E-4	0.0	0.0	29.348159271632777	0.0
36	2.03809775369018E-4	0.0	0.0	29.981314719794167	0.0
37	2.445717304428216E-4	0.0	0.0	30.63236466623296	0.0
38	3.260956405904288E-4	0.0	0.0	31.302735779376732	0.0
39	3.260956405904288E-4	0.0	0.0	32.03718468589653	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAC	20	7.034853E-4	45.000004	18
CGACGGT	45	3.8562575E-10	45.000004	30
TTCCGCG	45	1.9297659E-8	40.000004	1
TTTCGCG	75	0.0	39.0	1
TAGTCCG	35	6.251188E-6	38.571426	1
TTTAGCG	195	0.0	38.076923	1
CGAACCG	30	1.1402993E-4	37.500004	22
TCCGCGG	90	0.0	37.500004	2
ATCGTAG	30	1.1402993E-4	37.500004	1
ACGACAC	60	1.5643309E-10	37.500004	40
CGTAAGG	285	0.0	37.105263	2
CGTTTTT	2645	0.0	37.088844	1
TTAGTCG	55	2.752131E-9	36.81818	1
CTCGCGG	80	0.0	36.562504	2
GCGTTAG	155	0.0	36.290325	1
ATTCGCG	75	1.8189894E-12	36.0	1
CGTTTCG	25	0.002107574	36.0	42
TACGAAT	120	0.0	35.625004	12
TACGCGG	215	0.0	35.581394	2
TAACGTC	450	0.0	35.5	32
>>END_MODULE