FastQCFastQC Report
Sat 18 Jun 2016
SRR3552392_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552392_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1320310
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC60280.4565594443729124No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGCT46670.3534775923836069No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC38090.2884928539509661No Hit
TCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC25010.18942521074596116No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCC17940.13587718035915808No Hit
ACTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC15380.11648779453310207No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15030.11383690193969596No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA13410.10156705622164491No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACAG207.0336694E-445.01
TATAGCG302.1657488E-644.9999961
TATACGG750.042.02
ATACTCG700.041.78571344
CGAACTT603.6379788E-1241.24999614
TATTGCG1100.040.9090921
CGAATAT2100.040.71428714
TCCCGAT501.0822987E-940.50000439
GTACGAG800.039.3751
GAATCGT356.248996E-638.57142638
CGTCTAC356.248996E-638.57142641
TACGATA356.248996E-638.57142631
CGCCCTA356.248996E-638.57142617
GCTACGA2200.037.84090810
CCCGTAA301.1400142E-437.49999632
CAACTCG601.5643309E-1037.4999969
CGCAAAC601.5643309E-1037.49999620
TAGGGCG2650.037.358495
TACGAAT2300.037.17391612
ATAAGGG8550.037.1052633