##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3552392_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1320310 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.3667244813718 31.0 31.0 34.0 30.0 34.0 2 31.574999810650528 31.0 31.0 34.0 30.0 34.0 3 31.64049276306322 31.0 31.0 34.0 30.0 34.0 4 35.359972279237454 37.0 35.0 37.0 33.0 37.0 5 35.178989025304666 37.0 35.0 37.0 33.0 37.0 6 35.26867856791208 37.0 35.0 37.0 32.0 37.0 7 35.639255932318925 37.0 35.0 37.0 35.0 37.0 8 35.653347319947585 37.0 35.0 37.0 35.0 37.0 9 37.15965795911566 39.0 37.0 39.0 34.0 39.0 10 36.69854276647151 39.0 37.0 39.0 32.0 39.0 11 36.4252796691686 38.0 35.0 39.0 32.0 39.0 12 36.33528338041823 38.0 35.0 39.0 32.0 39.0 13 36.05249146033886 38.0 35.0 39.0 31.0 39.0 14 37.197995925199386 39.0 36.0 40.0 32.0 41.0 15 37.313397611167076 39.0 36.0 41.0 32.0 41.0 16 37.296093341715206 39.0 36.0 41.0 32.0 41.0 17 37.30617203535533 39.0 36.0 41.0 32.0 41.0 18 37.264073588778395 39.0 36.0 40.0 32.0 41.0 19 37.23403064431838 39.0 35.0 40.0 32.0 41.0 20 36.86758715756148 39.0 35.0 40.0 31.0 41.0 21 36.94021858502927 39.0 35.0 40.0 31.0 41.0 22 37.05300800569563 39.0 35.0 40.0 31.0 41.0 23 36.89279866092054 39.0 35.0 40.0 31.0 41.0 24 36.94323454340269 39.0 35.0 40.0 31.0 41.0 25 36.95829691511842 39.0 35.0 40.0 31.0 41.0 26 36.90568881550545 39.0 35.0 40.0 31.0 41.0 27 36.706638592451775 39.0 35.0 40.0 31.0 41.0 28 36.692435109936305 39.0 35.0 40.0 31.0 41.0 29 36.71271292348009 39.0 35.0 40.0 31.0 41.0 30 36.57646310336209 38.0 35.0 40.0 30.0 41.0 31 36.490786254743206 38.0 35.0 40.0 30.0 41.0 32 36.229096954503106 38.0 35.0 40.0 30.0 41.0 33 36.13942786163855 38.0 35.0 40.0 30.0 41.0 34 36.094986783406924 38.0 35.0 40.0 30.0 41.0 35 35.93074656709409 38.0 35.0 40.0 29.0 41.0 36 35.98074467359938 38.0 35.0 40.0 29.0 41.0 37 35.98545190144739 38.0 35.0 40.0 30.0 41.0 38 35.95839992123062 38.0 35.0 40.0 30.0 41.0 39 35.91146094477812 38.0 35.0 40.0 29.0 41.0 40 35.82467299346366 38.0 34.0 40.0 29.0 41.0 41 35.72121471472609 38.0 34.0 40.0 29.0 41.0 42 35.706862782225386 38.0 34.0 40.0 29.0 41.0 43 35.612439502844026 38.0 34.0 40.0 29.0 41.0 44 35.50639471033318 38.0 34.0 40.0 29.0 41.0 45 35.43070945459778 38.0 34.0 40.0 28.0 41.0 46 35.291164196287234 37.0 34.0 40.0 28.0 41.0 47 35.18187622603782 37.0 34.0 40.0 28.0 41.0 48 35.12931205550212 37.0 34.0 40.0 27.0 41.0 49 35.080294779256384 37.0 34.0 40.0 27.0 41.0 50 34.963098817701905 37.0 34.0 40.0 27.0 41.0 51 33.68783770478145 35.0 32.0 39.0 24.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 30.0 9 70.0 10 116.0 11 74.0 12 44.0 13 26.0 14 40.0 15 88.0 16 131.0 17 290.0 18 538.0 19 968.0 20 1635.0 21 2519.0 22 3687.0 23 5314.0 24 7350.0 25 9791.0 26 12525.0 27 15772.0 28 19352.0 29 24501.0 30 31269.0 31 40180.0 32 52271.0 33 70602.0 34 108639.0 35 120494.0 36 130320.0 37 173792.0 38 242936.0 39 244907.0 40 37.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.90273496375851 20.60470647045012 26.088191409593204 11.404367156198166 2 31.710431641053994 23.778582302641045 26.15984124940355 18.35114480690141 3 31.791851913565754 24.933841294847422 26.712590225022907 16.561716566563913 4 29.982579848671904 25.77281093076626 25.940347342669522 18.304261877892316 5 25.77432572653392 29.671592277571175 24.653149639099908 19.900932356794996 6 26.060773606198545 35.086002529708935 23.747074550673705 15.106149313418818 7 80.42164340192834 6.4689353257947 8.13952783815922 4.969893434117745 8 80.46595117813241 5.556422355355939 8.196559898811643 5.78106656770001 9 74.8161416637002 7.472487521869864 10.778453545000795 6.932917269429149 10 42.141921215472124 24.137134460846315 17.325855291560316 16.395089032121245 11 31.425044118426733 25.49370981057479 22.584771758147706 20.496474312850772 12 27.914732146238386 22.568411963856974 29.325007005930427 20.191848883974217 13 25.377146276253303 23.05337382887352 30.685596564443202 20.883883330429974 14 20.973483500087102 26.000106035703734 30.021964538631078 23.004445925578086 15 20.78655770235778 24.742749808757033 32.628170656891186 21.842521831994002 16 23.188645090925615 25.127583673531216 29.36158932371943 22.322181911823737 17 22.951579553286727 25.202263104876888 27.11454128197166 24.731616059864727 18 23.29642280979467 24.679128386515288 29.62963243480698 22.394816368883067 19 23.892343464792358 26.877551484121152 26.98593512129727 22.244169929789216 20 25.695253387462035 26.201498133014216 27.090077330323943 21.01317114919981 21 24.74487052283176 27.725079716127272 26.856798782104203 20.673250978936764 22 23.320280843135325 25.32208345009884 26.375093727988126 24.98254197877771 23 22.98535949890556 26.142951276594133 27.111738909801485 23.759950314698823 24 23.533488347433558 24.239080216009874 27.887693041785646 24.339738394770922 25 22.343086093417455 25.320265695177646 26.62685278457332 25.709795426831576 26 21.054222114503414 26.097280184199168 28.2534404798873 24.595057221410123 27 21.692102612265302 26.70054759867001 28.011906294733812 23.595443494330876 28 19.828449379312435 27.005248767334944 28.808916087888452 24.35738576546417 29 22.525316024267028 25.455991395960037 28.008876703198492 24.009815876574443 30 23.362164946111143 25.653520764062986 27.376373730411796 23.607940559414075 31 23.266051154653073 25.81802758443093 27.142943702615298 23.772977558300703 32 24.057910642197665 26.412054744719043 26.1127311010293 23.417303512053987 33 22.87281017336838 26.846270951518964 25.27186797040089 25.00905090471177 34 21.49646673887193 24.321409365982234 30.283948466647985 23.898175428497854 35 21.654232718073786 25.585733653460174 27.892010209723473 24.868023418742567 36 21.670971211306437 29.05673667547773 27.006612083525837 22.265680029689996 37 22.162219478758775 28.07651233422454 28.315774325726533 21.445493861290153 38 23.0762472449652 26.830820034688823 25.44334285130007 24.649589869045908 39 23.69223894388439 24.710333179329098 26.850133680726497 24.747294196060015 40 23.314979057948513 22.980587892237427 28.94380865099863 24.760624398815427 41 19.22041035817346 25.799471336277087 27.59291378539888 27.387204520150572 42 20.948565109709083 26.058349932970287 28.00637729018185 24.986707667138777 43 23.114723057463777 23.938241776552477 28.380910543735943 24.566124622247806 44 21.864865069566996 23.99709159212609 27.68811869939635 26.44992463891056 45 23.046784467284198 22.213419575705707 27.938590179579037 26.801205777431058 46 23.263627481424816 23.631192674447668 28.10847452492218 24.996705319205336 47 20.09073626648287 24.556581408911544 30.654921950148072 24.697760374457513 48 20.32234853935818 24.1996955260507 29.683862123289227 25.794093811301895 49 22.485476895577552 22.953700267361455 30.090357567540956 24.470465269520037 50 20.765199082033764 22.38648499216093 29.883739424832044 26.96457650097326 51 20.06271254478115 21.838886322151616 27.91465640645 30.183744726617235 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 467.0 1 902.0 2 1337.0 3 2430.0 4 3523.0 5 2725.5 6 1928.0 7 1986.0 8 2044.0 9 2263.5 10 2483.0 11 2672.5 12 2862.0 13 2943.5 14 3025.0 15 3198.5 16 3372.0 17 3607.5 18 3843.0 19 3915.0 20 3987.0 21 4133.0 22 4279.0 23 4978.0 24 5677.0 25 6830.5 26 8950.0 27 9916.0 28 11814.0 29 13712.0 30 16414.0 31 19116.0 32 21417.0 33 23718.0 34 26823.0 35 29928.0 36 31266.0 37 32604.0 38 36740.5 39 40877.0 40 45886.5 41 50896.0 42 60356.0 43 69816.0 44 85074.0 45 100332.0 46 117636.5 47 134941.0 48 134135.0 49 133329.0 50 116437.0 51 99545.0 52 89546.5 53 79548.0 54 75548.0 55 71548.0 56 68526.0 57 65504.0 58 66110.5 59 66717.0 60 63614.5 61 60512.0 62 56059.5 63 51607.0 64 44928.0 65 38249.0 66 32421.0 67 26593.0 68 23137.0 69 19681.0 70 16486.5 71 13292.0 72 11021.5 73 8751.0 74 7169.0 75 4550.0 76 3513.0 77 2578.0 78 1643.0 79 1337.0 80 1031.0 81 783.0 82 535.0 83 370.5 84 206.0 85 158.5 86 111.0 87 84.0 88 57.0 89 52.0 90 47.0 91 36.0 92 25.0 93 16.5 94 8.0 95 5.5 96 3.0 97 2.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1320310.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.77838215690619 #Duplication Level Percentage of deduplicated Percentage of total 1 70.67282358154384 19.631767015557365 2 11.669083070577923 6.482964979103957 3 4.621718319219627 3.851515730785709 4 2.4237570331733918 2.693121964919185 5 1.383142458032457 1.9210729888334086 6 0.8830956925738745 1.4718581777660897 7 0.6809299747906582 1.324059314327918 8 0.49206395498060335 1.0934992469671907 9 0.39831332475131764 0.9958049777816984 >10 5.9788525760748765 41.26276596253069 >50 0.7103609950406065 12.366194745495502 >100 0.07785287276082464 3.9434963795913913 >500 0.005245406314466813 0.9954985505828132 >1k 0.0024846661489579644 1.5040993045667295 >5k 2.760740165508849E-4 0.4622806611904474 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC 6028 0.4565594443729124 No Hit CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGCT 4667 0.3534775923836069 No Hit CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC 3809 0.2884928539509661 No Hit TCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC 2501 0.18942521074596116 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCC 1794 0.13587718035915808 No Hit ACTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC 1538 0.11648779453310207 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1503 0.11383690193969596 No Hit GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA 1341 0.10156705622164491 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.39369542001499647 0.0 2 0.0 0.0 0.0 1.5381993622709818 0.0 3 0.0 0.0 0.0 2.09859805651703 0.0 4 0.0 0.0 0.0 2.7392051866607083 0.0 5 0.0 0.0 0.0 4.308912300899031 0.0 6 0.0 0.0 0.0 5.2051412168354405 0.0 7 0.0 0.0 0.0 6.132196226643742 0.0 8 0.0 0.0 0.0 7.7384856586710695 0.0 9 0.0 0.0 0.0 8.288583741697026 0.0 10 0.0 0.0 0.0 9.670001742015133 0.0 11 0.0 0.0 0.0 11.750725208473767 0.0 12 0.0 0.0 0.0 13.227120903424195 0.0 13 0.0 0.0 0.0 13.80918117714779 0.0 14 0.0 0.0 0.0 14.045110617960933 0.0 15 0.0 0.0 0.0 14.491445190902137 0.0 16 0.0 0.0 0.0 15.409865864834774 0.0 17 0.0 0.0 0.0 16.503018230567065 0.0 18 0.0 0.0 0.0 17.730154281948938 0.0 19 0.0 0.0 0.0 18.465208928206255 0.0 20 0.0 0.0 0.0 19.157167634873627 0.0 21 2.2721936514909376E-4 0.0 0.0 19.97970173671335 0.0 22 2.2721936514909376E-4 0.0 0.0 20.821095045860442 0.0 23 3.786989419151563E-4 0.0 0.0 21.63507055161288 0.0 24 9.846172489794065E-4 0.0 0.0 22.313396096371306 0.0 25 0.0021964538631079064 0.0 0.0 22.911513205232104 0.0 26 0.0022721936514909376 0.0 0.0 23.442221902432003 0.0 27 0.0022721936514909376 0.0 0.0 23.95808560110883 0.0 28 0.0022721936514909376 0.0 0.0 24.48826411979005 0.0 29 0.0022721936514909376 0.0 0.0 25.04275511054222 0.0 30 0.0022721936514909376 0.0 0.0 25.66139770205482 0.0 31 0.0022721936514909376 0.0 0.0 26.214904075558014 0.0 32 0.0022721936514909376 0.0 0.0 26.758412796994644 0.0 33 0.0022721936514909376 0.0 0.0 27.288894274829396 0.0 34 0.002347933439873969 0.0 0.0 27.822405344199467 0.0 35 0.002347933439873969 0.0 0.0 28.40332952109732 0.0 36 0.002347933439873969 0.0 0.0 28.933659519355302 0.0 37 0.002347933439873969 0.0 0.0 29.485348137937304 0.0 38 0.002347933439873969 0.0 0.0 30.02802372170172 0.0 39 0.002347933439873969 0.0 0.0 30.624777514371626 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGACAG 20 7.0336694E-4 45.0 1 TATAGCG 30 2.1657488E-6 44.999996 1 TATACGG 75 0.0 42.0 2 ATACTCG 70 0.0 41.785713 44 CGAACTT 60 3.6379788E-12 41.249996 14 TATTGCG 110 0.0 40.909092 1 CGAATAT 210 0.0 40.714287 14 TCCCGAT 50 1.0822987E-9 40.500004 39 GTACGAG 80 0.0 39.375 1 GAATCGT 35 6.248996E-6 38.571426 38 CGTCTAC 35 6.248996E-6 38.571426 41 TACGATA 35 6.248996E-6 38.571426 31 CGCCCTA 35 6.248996E-6 38.571426 17 GCTACGA 220 0.0 37.840908 10 CCCGTAA 30 1.1400142E-4 37.499996 32 CAACTCG 60 1.5643309E-10 37.499996 9 CGCAAAC 60 1.5643309E-10 37.499996 20 TAGGGCG 265 0.0 37.35849 5 TACGAAT 230 0.0 37.173916 12 ATAAGGG 855 0.0 37.105263 3 >>END_MODULE