Basic Statistics
Measure | Value |
---|---|
Filename | SRR3552391_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1626464 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 49 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC | 5707 | 0.35088388061463394 | RNA PCR Primer, Index 14 (95% over 23bp) |
CTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGCT | 3500 | 0.21519074507643574 | RNA PCR Primer, Index 16 (95% over 24bp) |
CCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC | 3123 | 0.19201162767820254 | RNA PCR Primer, Index 16 (95% over 23bp) |
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA | 2669 | 0.16409831388828772 | No Hit |
TCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC | 2295 | 0.14110364570012002 | RNA PCR Primer, Index 14 (95% over 23bp) |
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 1733 | 0.10655016034784662 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CACGGTA | 20 | 7.034151E-4 | 45.000004 | 23 |
GTATACG | 35 | 1.2123382E-7 | 45.000004 | 1 |
ACGCGAA | 25 | 3.8915234E-5 | 45.0 | 37 |
TAATACG | 30 | 2.1660599E-6 | 44.999996 | 1 |
TTTAGCG | 225 | 0.0 | 41.0 | 1 |
CCCTCGT | 260 | 0.0 | 40.673077 | 14 |
TGTTACG | 50 | 1.0822987E-9 | 40.5 | 1 |
CGTTAGG | 310 | 0.0 | 39.919353 | 2 |
TAAACGG | 115 | 0.0 | 39.130432 | 2 |
ATTAGCG | 140 | 0.0 | 38.57143 | 1 |
GGTCGAA | 35 | 6.2498893E-6 | 38.57143 | 8 |
AAATGCG | 35 | 6.2498893E-6 | 38.57143 | 1 |
ACTATGC | 275 | 0.0 | 38.454544 | 8 |
CGTGTAC | 275 | 0.0 | 38.454544 | 18 |
TATACGG | 100 | 0.0 | 38.25 | 2 |
TTAGCGG | 535 | 0.0 | 37.850468 | 2 |
TATGCGT | 30 | 1.1401303E-4 | 37.499996 | 11 |
TCGATCA | 85 | 0.0 | 37.058823 | 17 |
TTATGCG | 80 | 0.0 | 36.562504 | 1 |
CCTCGTG | 290 | 0.0 | 36.46552 | 15 |