FastQCFastQC Report
Sat 18 Jun 2016
SRR3552391_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552391_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1626464
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC57070.35088388061463394RNA PCR Primer, Index 14 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGCT35000.21519074507643574RNA PCR Primer, Index 16 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC31230.19201162767820254RNA PCR Primer, Index 16 (95% over 23bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA26690.16409831388828772No Hit
TCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC22950.14110364570012002RNA PCR Primer, Index 14 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17330.10655016034784662No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGTA207.034151E-445.00000423
GTATACG351.2123382E-745.0000041
ACGCGAA253.8915234E-545.037
TAATACG302.1660599E-644.9999961
TTTAGCG2250.041.01
CCCTCGT2600.040.67307714
TGTTACG501.0822987E-940.51
CGTTAGG3100.039.9193532
TAAACGG1150.039.1304322
ATTAGCG1400.038.571431
GGTCGAA356.2498893E-638.571438
AAATGCG356.2498893E-638.571431
ACTATGC2750.038.4545448
CGTGTAC2750.038.45454418
TATACGG1000.038.252
TTAGCGG5350.037.8504682
TATGCGT301.1401303E-437.49999611
TCGATCA850.037.05882317
TTATGCG800.036.5625041
CCTCGTG2900.036.4655215