FastQCFastQC Report
Sat 18 Jun 2016
SRR3552390_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552390_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences958146
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC36700.3830313960502888TruSeq Adapter, Index 22 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC23670.2470395952182653TruSeq Adapter, Index 22 (95% over 22bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA21520.22460042623984236No Hit
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT17580.18347934448403477Illumina Paired End PCR Primer 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC14180.1479941470297846TruSeq Adapter, Index 22 (95% over 22bp)
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG12980.13546995969299042No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT11700.12211082653374328No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC11550.120545303116644No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG10710.1117783719808881No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10560.11021284856378881No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGTT207.032697E-445.00000429
GTCGAAC207.032697E-445.0000049
CCCGTCG207.032697E-445.00000430
ACGTGCG207.032697E-445.00000436
TATTACG207.032697E-445.00000418
TCGACGC207.032697E-445.00000412
CGGACGT207.032697E-445.00000433
ACGTTAT253.890319E-545.016
GCAACTA700.045.09
ACCCACG253.890319E-545.031
TACGTTA253.890319E-545.024
GTATTCG302.165123E-644.9999961
TGCTCGA302.165123E-644.9999961
TGCGTAG1100.042.9545441
TACGGGT1550.042.096774
ATGTACG700.041.7857131
TTAACGG1200.041.2499962
TAGTCGG556.002665E-1140.9090922
TACGGGA2750.040.909094
ATACTCG501.0804797E-940.544
Produced by FastQC (version 0.11.5)