SRR3552389_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552389_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1041549
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	3924	0.3767465572911116	TruSeq Adapter, Index 18 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT	3221	0.30925093298539	RNA PCR Primer, Index 34 (96% over 26bp)
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	2575	0.24722792686661887	RNA PCR Primer, Index 34 (96% over 25bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1919	0.18424481229399672	No Hit
TCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	1608	0.1543854393792323	TruSeq Adapter, Index 18 (95% over 23bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1279	0.12279787124753613	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1164	0.11175662402825023	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1136	0.10906832035746758	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATGCG	20	7.0329825E-4	45.0	1
CTACGAT	20	7.0329825E-4	45.0	20
TATAGCG	50	2.1827873E-11	45.0	1
TTACGAG	35	1.2117744E-7	45.0	1
TATACGC	20	7.0329825E-4	45.0	30
AAGTCGA	20	7.0329825E-4	45.0	12
CGGCATA	80	0.0	42.1875	39
CCCTCGT	215	0.0	39.76744	14
CGGGATC	120	0.0	39.374996	6
ATAGCGG	150	0.0	39.0	2
GCGTACG	65	9.094947E-12	38.07692	1
TCGTGTA	225	0.0	38.0	17
ATAATCG	90	0.0	37.5	34
AACTATG	210	0.0	37.5	7
TCGCCTA	30	1.1398483E-4	37.499996	21
TTTCGTC	30	1.1398483E-4	37.499996	19
AAACGTT	30	1.1398483E-4	37.499996	23
TATGGGA	455	0.0	37.08791	4
TACGCAG	85	0.0	37.058823	1
AGGGATC	365	0.0	36.986298	6