##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552389_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1041549
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.30292381827451	31.0	31.0	34.0	30.0	34.0
2	31.506967987103824	31.0	31.0	34.0	30.0	34.0
3	31.587549889635532	31.0	31.0	34.0	30.0	34.0
4	35.29346386967872	37.0	35.0	37.0	33.0	37.0
5	35.10228515413101	37.0	35.0	37.0	32.0	37.0
6	35.1918383100555	37.0	35.0	37.0	32.0	37.0
7	35.54387743639521	37.0	35.0	37.0	35.0	37.0
8	35.5520642811812	37.0	35.0	37.0	35.0	37.0
9	37.031901523596105	39.0	37.0	39.0	33.0	39.0
10	36.56969571282772	38.0	35.0	39.0	32.0	39.0
11	36.3012868333607	38.0	35.0	39.0	32.0	39.0
12	36.22736040263108	38.0	35.0	39.0	32.0	39.0
13	35.95958327452669	38.0	35.0	39.0	31.0	39.0
14	37.13638148565262	39.0	36.0	40.0	32.0	41.0
15	37.26876603981186	39.0	36.0	40.0	32.0	41.0
16	37.254165670554144	39.0	36.0	40.0	32.0	41.0
17	37.259996409194386	39.0	36.0	40.0	32.0	41.0
18	37.2323260835544	39.0	36.0	40.0	32.0	41.0
19	37.22607385730292	39.0	36.0	40.0	32.0	41.0
20	36.849573087775994	39.0	35.0	40.0	31.0	41.0
21	36.89860006586344	39.0	35.0	40.0	31.0	41.0
22	36.99362583997488	39.0	35.0	40.0	31.0	41.0
23	36.83610660660228	39.0	35.0	40.0	31.0	41.0
24	36.858179499956314	39.0	35.0	40.0	31.0	41.0
25	36.847790166377195	39.0	35.0	40.0	31.0	41.0
26	36.76862634403182	39.0	35.0	40.0	31.0	41.0
27	36.53122608729882	38.0	35.0	40.0	30.0	41.0
28	36.4731174433464	38.0	35.0	40.0	30.0	41.0
29	36.51924489390321	38.0	35.0	40.0	30.0	41.0
30	36.362114504454425	38.0	35.0	40.0	30.0	41.0
31	36.2419751735156	38.0	35.0	40.0	30.0	41.0
32	35.99201669820623	38.0	35.0	40.0	29.0	41.0
33	35.90864568061608	38.0	35.0	40.0	29.0	41.0
34	35.86859187613833	38.0	35.0	40.0	29.0	41.0
35	35.68537533999841	38.0	34.0	40.0	28.0	41.0
36	35.69903960351361	38.0	34.0	40.0	28.0	41.0
37	35.659107732809495	38.0	34.0	40.0	28.0	41.0
38	35.6459945715468	38.0	34.0	40.0	28.0	41.0
39	35.64021951919689	38.0	34.0	40.0	28.0	41.0
40	35.50940762268506	38.0	34.0	40.0	28.0	41.0
41	35.430142028843576	38.0	34.0	40.0	28.0	41.0
42	35.43819541855448	38.0	34.0	40.0	28.0	41.0
43	35.34264926566105	38.0	34.0	40.0	27.0	41.0
44	35.23743002009507	38.0	34.0	40.0	27.0	41.0
45	35.14397786373949	37.0	34.0	40.0	27.0	41.0
46	34.99552397438815	37.0	34.0	40.0	26.0	41.0
47	34.88435877716747	37.0	33.0	40.0	26.0	41.0
48	34.840812098134606	37.0	33.0	40.0	26.0	41.0
49	34.77715594753583	37.0	33.0	40.0	26.0	41.0
50	34.65500038884392	37.0	33.0	40.0	26.0	41.0
51	33.42688054042585	35.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	22.0
9	67.0
10	87.0
11	64.0
12	43.0
13	17.0
14	30.0
15	80.0
16	129.0
17	267.0
18	487.0
19	849.0
20	1546.0
21	2323.0
22	3349.0
23	4655.0
24	6556.0
25	8524.0
26	10728.0
27	13719.0
28	16841.0
29	21470.0
30	26822.0
31	34362.0
32	43719.0
33	57346.0
34	84075.0
35	94867.0
36	103476.0
37	136425.0
38	187987.0
39	180589.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.65598642022603	20.838193882380953	25.27495105847157	11.230868638921452
2	30.377447436462425	25.548966011200626	25.638832162481073	18.43475438985588
3	32.689772636717045	24.494382885490744	26.14720958879515	16.66863488899706
4	29.213219925322765	26.695911570170967	25.382579216148255	18.708289288358014
5	25.537348698909028	30.219413584958556	24.191852711682312	20.051385004450104
6	26.094307612987965	36.198392970469946	22.733736002818876	14.973563413723214
7	81.2240230656455	6.842404918059544	7.199373241201326	4.734198775093635
8	81.90473995942583	5.886520941405541	7.035962782355895	5.172776316812747
9	75.74314794599198	8.082769029589583	9.727242789345485	6.446840235072954
10	41.550421535616664	26.03977345280923	16.436960719082826	15.972844292491281
11	31.596689162007742	26.506290150535406	22.154214540074445	19.742806147382407
12	27.534374282919	24.415653992274965	28.224308217856287	19.825663506949745
13	23.91745371557171	26.306779613825178	29.43231667449155	20.34344999611156
14	20.364188338714744	28.609023675314365	29.05768235579891	21.969105630171985
15	20.82081591936625	27.018027956437958	31.829227429530437	20.33192869466535
16	22.5087825920816	25.8447754258321	29.32276829990716	22.323673682179138
17	22.31311248918678	25.919567874387088	27.30337218892246	24.46394744750367
18	23.309032988366365	25.857928911649857	28.32838397425373	22.504654125730042
19	24.16688989188219	26.897918388861207	26.149129805702852	22.786061913553755
20	25.63047921893257	26.700424079904067	26.941411301820654	20.72768539934271
21	24.761293035661307	28.17755093615375	26.473934495640627	20.587221532544316
22	23.294535350713215	26.62140715415213	25.14687259072785	24.937184904406802
23	23.2753331816362	26.88198058852728	26.19963151037541	23.64305471946111
24	22.899546732798935	26.022971554866835	27.608878698937833	23.468603013396393
25	22.603833329012847	27.73014039665921	25.661202689455802	24.00482358487215
26	22.60680966521978	28.01990112803142	26.773680354932893	22.5996088518159
27	23.5316821388144	27.66888547730352	26.795954871062232	22.003477512819845
28	22.309464077062145	27.44729244615472	27.397558828245238	22.845684648537897
29	24.465387610184447	25.78390454985795	26.135208233122015	23.615499606835588
30	23.883177843769232	26.511474736186198	25.93464157711255	23.67070584293202
31	25.78524870169334	28.68948076374707	23.331307504495708	22.193963030063877
32	25.862729453919115	28.16247723342829	23.263043793426906	22.71174951922569
33	23.721687601831505	27.056048251210456	23.764316417182485	25.457947729775555
34	23.23107218191367	27.14937079292477	25.330349316258765	24.289207708902797
35	24.919710930546714	26.14384920920667	24.8090104258177	24.12742943442891
36	24.835029364917062	27.939924093825642	25.032523673874202	22.1925228673831
37	24.751691951122798	28.315998575199053	25.646321008421115	21.285988465257034
38	23.82739554260049	28.57628397703805	24.410853450005714	23.18546703035575
39	24.569943420808816	25.565575887452248	25.374610315981293	24.489870375757647
40	23.86445572891914	26.695143483407886	26.483727601869905	22.956673185803066
41	21.307302872932528	27.52390910077202	25.16348246697947	26.00530555931598
42	23.209565752547405	25.547429837674464	26.469614007598295	24.773390402179828
43	24.940065229768354	23.605610489760924	27.122967810443864	24.331356470026854
44	23.552708513953736	24.251475446666458	26.02585188022839	26.169964159151416
45	24.079039968354827	24.735178085716562	25.423095792900767	25.762686153027847
46	23.54617977646755	26.546902738133298	26.227666677227862	23.67925080817129
47	21.88096767410847	25.69077402983441	28.35747526040542	24.070783035651708
48	21.70901225002376	25.532068102412847	27.85812285355754	24.90079679400585
49	23.506143253941968	24.414885905511888	28.31446240167289	23.764508438873257
50	21.86118943995914	24.674979285660108	27.81424589721655	25.649585377164204
51	22.0462983498616	24.24456266579873	25.679732782615122	28.029406201724548
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	385.0
1	766.0
2	1147.0
3	1676.5
4	2206.0
5	1761.5
6	1317.0
7	1292.0
8	1267.0
9	1352.0
10	1437.0
11	1586.5
12	1736.0
13	1895.0
14	2054.0
15	2152.0
16	2250.0
17	2317.5
18	2385.0
19	2812.0
20	3239.0
21	3287.0
22	3335.0
23	4121.5
24	4908.0
25	6812.5
26	9005.5
27	9294.0
28	12009.5
29	14725.0
30	16565.5
31	18406.0
32	19748.0
33	21090.0
34	22374.0
35	23658.0
36	26030.0
37	28402.0
38	30687.0
39	32972.0
40	37237.0
41	41502.0
42	45544.0
43	49586.0
44	54587.5
45	59589.0
46	62641.0
47	65693.0
48	74978.5
49	84264.0
50	93661.5
51	103059.0
52	100712.5
53	98366.0
54	85523.5
55	72681.0
56	64943.5
57	57206.0
58	52706.5
59	48207.0
60	46713.0
61	45219.0
62	41257.0
63	37295.0
64	32014.5
65	26734.0
66	23512.5
67	20291.0
68	17767.0
69	15243.0
70	13433.5
71	11624.0
72	9545.0
73	7466.0
74	6403.0
75	4371.0
76	3402.0
77	2752.5
78	2103.0
79	1523.5
80	944.0
81	669.5
82	395.0
83	320.0
84	245.0
85	158.0
86	71.0
87	59.0
88	47.0
89	31.0
90	15.0
91	15.0
92	15.0
93	16.0
94	17.0
95	8.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1041549.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.257305120664505
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.17224761579465	19.944782796285303
2	10.97433021760979	5.98261334472637
3	4.0741726192557985	3.3315289859193657
4	1.9228776986639502	2.0964985656881785
5	1.0703096570352164	1.4586878447701335
6	0.734208313649446	1.2007524016362898
7	0.5102827328572356	0.9736252503107343
8	0.4064810849107898	0.8863663165753708
9	0.3266111565201327	0.8012285954174131
>10	5.823937533256263	41.80921513156799
>50	0.9054488156129383	15.356286286131821
>100	0.07229274242742174	3.7089397757203364
>500	0.0032209637715187294	0.6122223516588357
>1k	0.00357884863502081	1.8372523535918597
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	3924	0.3767465572911116	TruSeq Adapter, Index 18 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT	3221	0.30925093298539	RNA PCR Primer, Index 34 (96% over 26bp)
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	2575	0.24722792686661887	RNA PCR Primer, Index 34 (96% over 25bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1919	0.18424481229399672	No Hit
TCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	1608	0.1543854393792323	TruSeq Adapter, Index 18 (95% over 23bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1279	0.12279787124753613	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1164	0.11175662402825023	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1136	0.10906832035746758	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.60108453850947E-5	0.0	0.0	0.36445716908181947	0.0
2	9.60108453850947E-5	0.0	0.0	1.3753553601414816	0.0
3	9.60108453850947E-5	0.0	0.0	1.8101884788905755	0.0
4	9.60108453850947E-5	0.0	0.0	2.3283590114339314	0.0
5	9.60108453850947E-5	0.0	0.0	3.583892836534815	0.0
6	9.60108453850947E-5	0.0	0.0	4.270850435265167	0.0
7	9.60108453850947E-5	0.0	0.0	5.032888515086664	0.0
8	9.60108453850947E-5	0.0	0.0	6.273348637462088	0.0
9	9.60108453850947E-5	0.0	0.0	6.709909951428114	0.0
10	9.60108453850947E-5	0.0	0.0	7.8696249528346724	0.0
11	9.60108453850947E-5	0.0	0.0	9.483759285448885	0.0
12	9.60108453850947E-5	0.0	0.0	10.71221805215117	0.0
13	9.60108453850947E-5	0.0	0.0	11.201489320233614	0.0
14	9.60108453850947E-5	0.0	0.0	11.403112095542312	0.0
15	9.60108453850947E-5	0.0	0.0	11.78091477213266	0.0
16	9.60108453850947E-5	0.0	0.0	12.57204413810584	0.0
17	9.60108453850947E-5	0.0	0.0	13.48885170068811	0.0
18	9.60108453850947E-5	0.0	0.0	14.525960852537903	0.0
19	9.60108453850947E-5	0.0	0.0	15.11517941066623	0.0
20	9.60108453850947E-5	0.0	0.0	15.672426357281319	0.0
21	0.0010561192992360417	0.0	0.0	16.346614513575453	0.0
22	0.0011521301446211364	0.0	0.0	17.039716806410453	0.0
23	0.0011521301446211364	0.0	0.0	17.691246403193706	0.0
24	0.00163218437154661	0.0	0.0	18.265871312823496	0.0
25	0.001920216907701894	0.0	0.0	18.757926895422106	0.0
26	0.0021122385984720833	0.0	0.0	19.206105521679728	0.0
27	0.0021122385984720833	0.0	0.0	19.63162558842647	0.0
28	0.0021122385984720833	0.0	0.0	20.07375553142483	0.0
29	0.0021122385984720833	0.0	0.0	20.54795309678181	0.0
30	0.0021122385984720833	0.0	0.0	21.067563792005945	0.0
31	0.0022082494438571782	0.0	0.0	21.553954734726833	0.0
32	0.0022082494438571782	0.0	0.0	22.03439300503385	0.0
33	0.0022082494438571782	0.0	0.0	22.487180151869957	0.0
34	0.002496281980012462	0.0	0.0	22.952736741142278	0.0
35	0.002496281980012462	0.0	0.0	23.431830859613903	0.0
36	0.002496281980012462	0.0	0.0	23.889994613791576	0.0
37	0.0026883036707826516	0.0	0.0	24.344701977535383	0.0
38	0.002784314516167746	0.0	0.0	24.80728223060077	0.0
39	0.002880325361552841	0.0	0.0	25.263813800406894	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATGCG	20	7.0329825E-4	45.0	1
CTACGAT	20	7.0329825E-4	45.0	20
TATAGCG	50	2.1827873E-11	45.0	1
TTACGAG	35	1.2117744E-7	45.0	1
TATACGC	20	7.0329825E-4	45.0	30
AAGTCGA	20	7.0329825E-4	45.0	12
CGGCATA	80	0.0	42.1875	39
CCCTCGT	215	0.0	39.76744	14
CGGGATC	120	0.0	39.374996	6
ATAGCGG	150	0.0	39.0	2
GCGTACG	65	9.094947E-12	38.07692	1
TCGTGTA	225	0.0	38.0	17
ATAATCG	90	0.0	37.5	34
AACTATG	210	0.0	37.5	7
TCGCCTA	30	1.1398483E-4	37.499996	21
TTTCGTC	30	1.1398483E-4	37.499996	19
AAACGTT	30	1.1398483E-4	37.499996	23
TATGGGA	455	0.0	37.08791	4
TACGCAG	85	0.0	37.058823	1
AGGGATC	365	0.0	36.986298	6
>>END_MODULE