FastQCFastQC Report
Sat 18 Jun 2016
SRR3552386_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552386_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2169054
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC60270.2778630684160007No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT43790.2018852458260606No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC43290.19958009344165703No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC34300.1581334535700817No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA28150.12978007924191837No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG27810.128212575620524No Hit
TCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC24160.11138496321437825No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22080.10179552929525959No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATCG253.891954E-545.01
TTATCCG351.2125929E-745.01
CGTAGAT207.034673E-445.012
ACGTCGA207.034673E-445.028
TCGTAAG750.042.0000041
ACGTTAG650.041.538461
TCGATAG556.184564E-1140.9090921
ATTCGCG403.460118E-739.3751
ACACGAC8900.038.67977526
AACACGT8550.038.15789441
CGAATAT2300.038.15217214
GCTACGA2200.037.84090810
GCGAGAC9400.037.5797921
TCTCGAC301.1402556E-437.50000431
CGTTTAG301.1402556E-437.5000043
CGAGACA9450.037.38095522
ACACGTG8750.037.28571342
CACGACC9300.037.25806427
CGTCATA850.037.05882338
CTACGAA2250.037.011