##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552386_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2169054
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.37856641651153	31.0	31.0	34.0	30.0	34.0
2	31.577499684194123	31.0	31.0	34.0	30.0	34.0
3	31.662964130906836	31.0	31.0	34.0	30.0	34.0
4	35.359250161591184	37.0	35.0	37.0	33.0	37.0
5	35.163244898467255	37.0	35.0	37.0	33.0	37.0
6	35.258455068430756	37.0	35.0	37.0	32.0	37.0
7	35.621142212227085	37.0	35.0	37.0	35.0	37.0
8	35.633158049546026	37.0	35.0	37.0	35.0	37.0
9	37.10067568626692	39.0	37.0	39.0	34.0	39.0
10	36.622021397346494	38.0	35.0	39.0	32.0	39.0
11	36.362653027541036	38.0	35.0	39.0	32.0	39.0
12	36.32437873838088	38.0	35.0	39.0	32.0	39.0
13	36.0706123499	38.0	35.0	39.0	31.0	39.0
14	37.26243883278148	39.0	36.0	41.0	32.0	41.0
15	37.37234434919555	39.0	36.0	41.0	32.0	41.0
16	37.341083716680174	39.0	36.0	41.0	32.0	41.0
17	37.33966604796377	39.0	36.0	41.0	32.0	41.0
18	37.314633937191054	39.0	36.0	41.0	32.0	41.0
19	37.281546701926274	39.0	36.0	41.0	32.0	41.0
20	36.89716069770508	39.0	35.0	40.0	31.0	41.0
21	36.98755586536804	39.0	35.0	40.0	31.0	41.0
22	37.07571595727907	39.0	35.0	40.0	31.0	41.0
23	36.90768464040084	39.0	35.0	40.0	31.0	41.0
24	36.94274139786285	39.0	35.0	40.0	31.0	41.0
25	36.95552254577341	39.0	35.0	40.0	31.0	41.0
26	36.905686534314036	39.0	35.0	40.0	31.0	41.0
27	36.6887929023436	39.0	35.0	40.0	31.0	41.0
28	36.63255732683465	39.0	35.0	40.0	30.0	41.0
29	36.67443733535449	39.0	35.0	40.0	30.0	41.0
30	36.55695432202241	39.0	35.0	40.0	30.0	41.0
31	36.46899662249073	38.0	35.0	40.0	30.0	41.0
32	36.2297545381535	38.0	35.0	40.0	30.0	41.0
33	36.16671691898865	38.0	35.0	40.0	30.0	41.0
34	36.09665734463042	38.0	35.0	40.0	29.0	41.0
35	35.94281516273915	38.0	35.0	40.0	29.0	41.0
36	35.996172986011416	38.0	35.0	40.0	29.0	41.0
37	36.016207065384265	38.0	35.0	40.0	29.0	41.0
38	35.966501986580326	38.0	35.0	40.0	29.0	41.0
39	35.9287256103352	38.0	35.0	40.0	29.0	41.0
40	35.80663598047813	38.0	34.0	40.0	29.0	41.0
41	35.74726724184829	38.0	34.0	40.0	29.0	41.0
42	35.753906080715375	38.0	34.0	40.0	29.0	41.0
43	35.67816799397341	38.0	34.0	40.0	29.0	41.0
44	35.56625100158871	38.0	34.0	40.0	28.0	41.0
45	35.471696877993814	38.0	34.0	40.0	28.0	41.0
46	35.33752179521579	37.0	34.0	40.0	28.0	41.0
47	35.245382088228325	37.0	34.0	40.0	28.0	41.0
48	35.197648375743526	37.0	34.0	40.0	27.0	41.0
49	35.12456951279221	37.0	34.0	40.0	27.0	41.0
50	35.018196411892006	37.0	34.0	40.0	27.0	41.0
51	33.79857117434605	36.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	10.0
8	41.0
9	118.0
10	159.0
11	130.0
12	61.0
13	56.0
14	45.0
15	99.0
16	196.0
17	434.0
18	832.0
19	1450.0
20	2493.0
21	3922.0
22	5928.0
23	8460.0
24	11659.0
25	16230.0
26	20704.0
27	26511.0
28	33172.0
29	41974.0
30	53795.0
31	67978.0
32	86529.0
33	114148.0
34	168025.0
35	189349.0
36	215430.0
37	285833.0
38	405130.0
39	408110.0
40	43.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.441703157228915	20.195670554997708	25.299831170639365	11.062795117134014
2	30.30298000879646	24.80666686952008	25.999076094924334	18.89127702675913
3	33.219827629925305	24.277726603394846	26.067308605502674	16.435137161177177
4	29.97790741954788	26.230098466889253	24.836772159660388	18.955221953902484
5	25.67815277996767	30.521923382267108	23.84560273741456	19.95432110035066
6	26.083536878288875	36.17844461225953	22.57998187228165	15.158036637169936
7	82.60204679090516	6.476878860553955	6.718919860916325	4.202154487624559
8	83.75595997148989	5.464916963800809	6.455118222045186	4.324004842664129
9	77.10854593753774	7.9158932880416994	8.803561368227808	6.171999406192746
10	40.76159468597832	27.228183346288287	16.185028127469394	15.825193840264005
11	31.655044088344503	26.940408122619353	21.819373791523862	19.58517399751228
12	27.014864544635586	24.65148401100203	28.27794974214565	20.055701702216727
13	24.590535781958405	25.210713979458326	29.782661012588896	20.416089225994373
14	21.45894016469853	27.671510252856773	28.498184000951564	22.37136558149313
15	22.17828601777549	25.862611073767646	30.812556994892702	21.146545913564164
16	24.244809027345564	26.09367954878025	28.695274529818064	20.966236894056117
17	23.890645415005803	25.737210784056092	27.19107039289939	23.181073408038714
18	24.788778887017106	25.324035270675605	27.827615172328585	22.059570669978708
19	24.859731477409046	26.563930635198567	26.301650396901138	22.274687490491246
20	26.373156223865337	26.597217035629356	26.581219278081598	20.448407462423713
21	25.406974653466442	27.59636228512522	26.323411035409904	20.67325202599843
22	23.931354406114373	25.27774781079678	26.13475736427032	24.656140418818527
23	24.10871283057038	26.598738436203064	26.472923219062324	22.81962551416424
24	22.916211399070747	25.71194631392303	27.89257436652107	23.479267920485153
25	23.092094526000736	27.37492934707942	25.882389281225826	23.650586845694022
26	22.761212952743453	27.709775782437873	26.255178524831564	23.27383273998711
27	23.358754553828533	26.886329247681246	26.3450794678233	23.409836730666918
28	20.468600597311084	28.822426735341764	27.283968033990856	23.42500463335629
29	22.49750351996769	27.832824816717334	25.60913651757863	24.060535145736345
30	23.13446322682607	27.26372879605579	25.52218616963893	24.079621807479203
31	23.871420444119877	29.436749845785304	23.352484539342957	23.33934517075186
32	22.306221975109885	28.978531654813573	24.335171000814178	24.38007536926236
33	22.25315736722092	28.061449830202474	23.867040654589513	25.818352147987095
34	20.722812802263107	29.517061354857926	25.897926008296707	23.862199834582263
35	21.758195047241795	27.24782324460341	25.406513622989564	25.587468085165238
36	21.565161586571843	30.86262490468195	24.779281659193366	22.792931849552847
37	21.900238537168736	29.941900939303494	24.76356051993173	23.39430000359604
38	20.75886538555518	29.84098136791431	24.872640330761705	24.52751291576881
39	22.819717720259618	27.03538040085678	26.009403177606462	24.135498701277147
40	22.316041924267445	27.533385521983316	26.79315498830366	23.35741756544558
41	19.77797694294379	27.918760897607896	25.988472393956073	26.314789765492236
42	21.4969290759935	26.80790796356384	26.66747808030598	25.027684880136686
43	22.62105968777172	24.68066724018858	27.41623767780793	25.282035394231773
44	21.614076920168884	26.76157440063733	25.651044187927084	25.973304491266703
45	22.412950530507768	25.902997343542395	25.52389198240339	26.160160143546452
46	21.517998168786946	27.61558725601115	25.612963070536743	25.25345150466517
47	20.45924167863041	27.281155748081883	28.163983008260747	24.095619565026965
48	20.450666511760428	26.51160367607261	27.591705877308726	25.446023934858236
49	21.731363073487337	26.12433807549282	28.150474815288142	23.993824035731706
50	20.076217558437918	26.287081833831706	27.946975962792997	25.689724644937378
51	20.858586277704475	25.42827426149833	25.527995153647627	28.185144307149567
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	658.0
1	1228.5
2	1799.0
3	3316.5
4	4834.0
5	3675.0
6	2516.0
7	2573.5
8	2631.0
9	2724.5
10	2818.0
11	2974.5
12	3131.0
13	3532.5
14	3934.0
15	4139.0
16	4344.0
17	4669.5
18	4995.0
19	5467.0
20	5939.0
21	6894.0
22	7849.0
23	8205.0
24	8561.0
25	11835.5
26	17331.5
27	19553.0
28	23819.0
29	28085.0
30	33562.5
31	39040.0
32	41739.0
33	44438.0
34	48364.5
35	52291.0
36	57146.0
37	62001.0
38	67319.5
39	72638.0
40	84286.5
41	95935.0
42	106317.0
43	116699.0
44	135457.5
45	154216.0
46	176146.0
47	198076.0
48	201913.5
49	205751.0
50	185007.5
51	164264.0
52	151380.5
53	138497.0
54	130244.0
55	121991.0
56	117021.5
57	112052.0
58	107187.0
59	102322.0
60	97701.5
61	93081.0
62	88149.0
63	83217.0
64	72531.5
65	61846.0
66	52510.5
67	43175.0
68	37390.5
69	31606.0
70	26751.0
71	21896.0
72	18073.5
73	14251.0
74	12149.5
75	8218.5
76	6389.0
77	4806.0
78	3223.0
79	2582.5
80	1942.0
81	1334.5
82	727.0
83	560.0
84	393.0
85	299.5
86	206.0
87	129.5
88	53.0
89	36.0
90	19.0
91	13.5
92	8.0
93	6.5
94	5.0
95	2.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2169054.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.176256823943664
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.29165124228143	16.993890130124747
2	11.830550146239144	5.7203683740804365
3	4.593792735679523	3.3318213892126476
4	2.288364239754012	2.212963262680863
5	1.29774898508049	1.5687356378159083
6	0.8528849608367818	1.2371739512681503
7	0.6392061482759683	1.0817528402914536
8	0.47825083870908597	0.9249852082317855
9	0.37697218912456515	0.8202398813783776
>10	5.878513553676493	37.530842044356675
>50	1.3620878303059492	21.291993882066393
>100	0.10003244015568895	4.240081006127279
>500	0.006434799334659255	0.9911182583340453
>1k	0.0033148966269456763	1.7699079095744574
>5k	1.949939192320986E-4	0.2841262244567775
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	6027	0.2778630684160007	No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT	4379	0.2018852458260606	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	4329	0.19958009344165703	No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	3430	0.1581334535700817	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	2815	0.12978007924191837	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2781	0.128212575620524	No Hit
TCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	2416	0.11138496321437825	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2208	0.10179552929525959	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.220609537614094E-5	0.0	0.0	0.22968538358196708	0.0
2	9.220609537614094E-5	0.0	0.0	0.9240894878596845	0.0
3	9.220609537614094E-5	0.0	0.0	1.2526659087325627	0.0
4	9.220609537614094E-5	0.0	0.0	1.6366120898788135	0.0
5	9.220609537614094E-5	0.0	0.0	2.64045985023886	0.0
6	9.220609537614094E-5	0.0	0.0	3.238324172657758	0.0
7	9.220609537614094E-5	0.0	0.0	3.8458701350911504	0.0
8	9.220609537614094E-5	0.0	0.0	4.873046037581361	0.0
9	9.220609537614094E-5	0.0	0.0	5.23647636250642	0.0
10	9.220609537614094E-5	0.0	0.0	6.207452649864872	0.0
11	9.220609537614094E-5	0.0	0.0	7.602300357667444	0.0
12	9.220609537614094E-5	0.0	0.0	8.637313778264627	0.0
13	9.220609537614094E-5	0.0	0.0	9.065749400429866	0.0
14	9.220609537614094E-5	0.0	0.0	9.232458020869927	0.0
15	9.220609537614094E-5	0.0	0.0	9.568641444611337	0.0
16	9.220609537614094E-5	0.0	0.0	10.275308959574081	0.0
17	9.220609537614094E-5	0.0	0.0	11.09944704004603	0.0
18	9.220609537614094E-5	0.0	0.0	11.979047086886725	0.0
19	9.220609537614094E-5	0.0	0.0	12.50194785376482	0.0
20	1.383091430642114E-4	0.0	0.0	13.033147169226769	0.0
21	1.8441219075228187E-4	0.0	0.0	13.695048624884397	0.0
22	1.8441219075228187E-4	0.0	0.0	14.363773331599859	0.0
23	1.8441219075228187E-4	0.0	0.0	15.033143480982954	0.0
24	1.8441219075228187E-4	0.0	0.0	15.578081504655946	0.0
25	3.2272133381649326E-4	0.0	0.0	16.072951618539694	0.0
26	3.6882438150456374E-4	0.0	0.0	16.528127008364017	0.0
27	3.6882438150456374E-4	0.0	0.0	16.95826844329371	0.0
28	3.6882438150456374E-4	0.0	0.0	17.419483332365168	0.0
29	3.6882438150456374E-4	0.0	0.0	17.89923164660723	0.0
30	3.6882438150456374E-4	0.0	0.0	18.462149858878572	0.0
31	3.6882438150456374E-4	0.0	0.0	18.9566972514285	0.0
32	3.6882438150456374E-4	0.0	0.0	19.47973632744966	0.0
33	4.149274291926342E-4	0.0	0.0	19.984610802681722	0.0
34	4.6103047688070465E-4	0.0	0.0	20.488148289530827	0.0
35	4.6103047688070465E-4	0.0	0.0	21.00247388953894	0.0
36	4.6103047688070465E-4	0.0	0.0	21.502922472192946	0.0
37	4.6103047688070465E-4	0.0	0.0	21.970315169654604	0.0
38	4.6103047688070465E-4	0.0	0.0	22.46864301211496	0.0
39	4.6103047688070465E-4	0.0	0.0	22.96411246561865	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATATCG	25	3.891954E-5	45.0	1
TTATCCG	35	1.2125929E-7	45.0	1
CGTAGAT	20	7.034673E-4	45.0	12
ACGTCGA	20	7.034673E-4	45.0	28
TCGTAAG	75	0.0	42.000004	1
ACGTTAG	65	0.0	41.53846	1
TCGATAG	55	6.184564E-11	40.909092	1
ATTCGCG	40	3.460118E-7	39.375	1
ACACGAC	890	0.0	38.679775	26
AACACGT	855	0.0	38.157894	41
CGAATAT	230	0.0	38.152172	14
GCTACGA	220	0.0	37.840908	10
GCGAGAC	940	0.0	37.57979	21
TCTCGAC	30	1.1402556E-4	37.500004	31
CGTTTAG	30	1.1402556E-4	37.500004	3
CGAGACA	945	0.0	37.380955	22
ACACGTG	875	0.0	37.285713	42
CACGACC	930	0.0	37.258064	27
CGTCATA	85	0.0	37.058823	38
CTACGAA	225	0.0	37.0	11
>>END_MODULE