##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552384_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1340176
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.431171726698583	31.0	31.0	34.0	30.0	34.0
2	31.645824876732608	31.0	31.0	34.0	30.0	34.0
3	31.717782589749405	31.0	31.0	34.0	30.0	34.0
4	35.43201713804754	37.0	35.0	37.0	33.0	37.0
5	35.23395061544156	37.0	35.0	37.0	33.0	37.0
6	35.323147855207075	37.0	35.0	37.0	32.0	37.0
7	35.665458865104284	37.0	35.0	37.0	35.0	37.0
8	35.66630949964781	37.0	35.0	37.0	35.0	37.0
9	37.14716276071203	39.0	37.0	39.0	34.0	39.0
10	36.73575410990795	39.0	37.0	39.0	32.0	39.0
11	36.482544083762136	38.0	35.0	39.0	32.0	39.0
12	36.445672807153684	38.0	35.0	39.0	32.0	39.0
13	36.18887220782942	38.0	35.0	39.0	32.0	39.0
14	37.39332968207161	39.0	36.0	41.0	32.0	41.0
15	37.49343742911379	39.0	36.0	41.0	32.0	41.0
16	37.472584944067044	39.0	36.0	41.0	32.0	41.0
17	37.498603168539056	39.0	36.0	41.0	32.0	41.0
18	37.457238452262985	39.0	36.0	41.0	32.0	41.0
19	37.44364695383293	39.0	36.0	41.0	32.0	41.0
20	37.05523080550614	39.0	35.0	41.0	31.0	41.0
21	37.133454859660226	39.0	35.0	40.0	31.0	41.0
22	37.21487849357099	39.0	35.0	40.0	32.0	41.0
23	37.066104004250185	39.0	35.0	40.0	31.0	41.0
24	37.10922446007092	39.0	35.0	40.0	31.0	41.0
25	37.10326852592495	39.0	35.0	40.0	31.0	41.0
26	37.06574808084908	39.0	35.0	40.0	31.0	41.0
27	36.88215876123733	39.0	35.0	40.0	31.0	41.0
28	36.859891536633995	39.0	35.0	40.0	31.0	41.0
29	36.91460375353685	39.0	35.0	40.0	31.0	41.0
30	36.78756073829109	39.0	35.0	40.0	31.0	41.0
31	36.68053822781486	39.0	35.0	40.0	31.0	41.0
32	36.40743827676365	39.0	35.0	40.0	30.0	41.0
33	36.319998268884085	38.0	35.0	40.0	30.0	41.0
34	36.273264108594695	38.0	35.0	40.0	30.0	41.0
35	36.103101383698856	38.0	35.0	40.0	30.0	41.0
36	36.15626529649837	38.0	35.0	40.0	30.0	41.0
37	36.186588179462994	38.0	35.0	40.0	30.0	41.0
38	36.11703313594632	38.0	35.0	40.0	30.0	41.0
39	36.06722922959373	38.0	35.0	40.0	30.0	41.0
40	35.94146888169912	38.0	35.0	40.0	29.0	41.0
41	35.85192541875097	38.0	35.0	40.0	29.0	41.0
42	35.84749316507683	38.0	35.0	40.0	29.0	41.0
43	35.78409029858765	38.0	34.0	40.0	29.0	41.0
44	35.70838009336087	38.0	34.0	40.0	29.0	41.0
45	35.63887877413116	38.0	34.0	40.0	29.0	41.0
46	35.497780142309665	38.0	34.0	40.0	28.0	41.0
47	35.40220687432098	38.0	34.0	40.0	28.0	41.0
48	35.32312920094077	37.0	34.0	40.0	28.0	41.0
49	35.263566874798535	37.0	34.0	40.0	28.0	41.0
50	35.148587946657756	37.0	34.0	40.0	27.0	41.0
51	33.93489362591182	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	35.0
9	98.0
10	104.0
11	90.0
12	46.0
13	41.0
14	32.0
15	69.0
16	121.0
17	256.0
18	518.0
19	908.0
20	1504.0
21	2376.0
22	3443.0
23	5044.0
24	7165.0
25	9151.0
26	11608.0
27	14690.0
28	18822.0
29	23714.0
30	30191.0
31	38779.0
32	51221.0
33	68380.0
34	103147.0
35	117521.0
36	130344.0
37	177438.0
38	254292.0
39	268994.0
40	30.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.119112713554046	21.320259428612363	24.712724298898056	10.847903558935542
2	30.96063502107186	25.773182029822948	25.366668258497395	17.899514690607802
3	31.482805243490407	24.486559974212344	26.822148732703766	17.208486049593485
4	29.53753835317152	26.07948508255632	25.47172908632896	18.911247477943196
5	25.71774155036353	30.234088657012215	24.332774202791274	19.715395589832976
6	25.572462124377694	36.129284511885004	23.336114062630582	14.962139301106719
7	80.94757703465814	6.630099330237223	7.645339119638017	4.776984515466625
8	81.26343107173983	5.973319922159478	7.58504853093922	5.178200475161471
9	74.97336170771601	8.575813922947434	9.95772197084562	6.493102398490945
10	40.3329861152565	26.10291484103581	17.352944687861893	16.2111543558458
11	29.637674454698487	26.040982676902136	23.824557371569107	20.496785496830267
12	26.311320304198855	23.58309654851303	30.205211852771573	19.900371294516543
13	23.183372930122612	25.09938993087475	31.37035732620193	20.346879812800704
14	20.302557276059265	27.648756581225154	29.872046656558542	22.176639486157043
15	20.400902568020918	26.517114170079154	32.26397129929203	20.818011962607898
16	22.107618700827352	25.79377633982402	29.89234249829873	22.206262461049892
17	22.413623285299842	25.982035195377325	27.65853141676914	23.945810102553693
18	21.89593008679457	26.679928606391996	29.769373574814058	21.65476773199938
19	23.375884958393524	26.70044829932785	27.728522223946705	22.195144518331922
20	24.161378803977986	26.92803034825277	27.71315110851112	21.19743973925813
21	23.81791645276441	27.73053688470768	27.87514475710653	20.576401905421378
22	22.14686727713375	26.0397888038586	26.900869736512217	24.912474182495433
23	22.431829849213834	26.235658600064472	27.808810186124806	23.52370136459689
24	22.402729193777535	26.081201275056408	28.323966404412555	23.1921031267535
25	21.293098816871815	27.68487124079225	26.74096536574301	24.281064576592925
26	20.573193371616863	27.623759864376023	28.54729528061986	23.255751483387257
27	21.094468189252755	27.48049509915116	29.004847124556775	22.420189587039314
28	18.867596494788742	26.8510255369444	30.466819283437403	23.814558684829457
29	20.54692816465897	25.30055753871133	28.898368572486	25.254145724143694
30	21.59679027232244	26.019269110922743	28.58266376953475	23.801276847220066
31	23.01951385489667	27.386328362841894	26.303187044089732	23.290970738171705
32	22.06307231289025	27.884024187867862	26.480477191055503	23.572426308186387
33	21.917643652773965	27.41804061556094	25.836009598739267	24.828306132925825
34	20.933668413700886	25.73065030264682	28.863000083571112	24.472681200081183
35	21.540678239276033	25.425391888826542	27.809705590907463	25.224224280989958
36	21.711476701567555	28.327995725934503	28.25972111125703	21.700806461240912
37	21.846682823748523	27.270074975227132	29.011264192165804	21.87197800885854
38	20.47186339704636	28.71063203638925	26.321393608003724	24.496110958560667
39	23.001754993374004	25.866378744284336	26.83363976074784	24.29822650159382
40	23.264332445887703	25.628872625684984	28.15413796396891	22.9526569644584
41	20.333374123995654	26.96086185695013	27.591749143396093	25.114014875658125
42	20.42821241389191	26.940416781079502	28.972836403576846	23.65853440145175
43	21.574927472212604	24.085866333974046	30.069408794068842	24.269797399744512
44	20.59811547140077	24.188539415718534	28.506405128878594	26.706939984002105
45	21.943013454949202	23.828959778417165	27.60144936187486	26.626577404758777
46	21.97159179092895	25.733933453516556	28.46200797507193	23.832466780482562
47	20.75406513771326	25.22668664414226	30.54173481691957	23.477513401224915
48	19.617274149066986	25.4217356526307	30.140444240159503	24.820545958142812
49	21.1387907259942	24.94948424684519	30.202973340814935	23.708751686345675
50	20.149443058225188	23.949391721684314	29.937187354496725	25.963977865593773
51	20.56259774835544	23.34133725719607	27.71934432492449	28.376720669524
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	408.0
1	869.0
2	1330.0
3	2389.5
4	3449.0
5	2630.0
6	1811.0
7	1883.0
8	1955.0
9	2101.0
10	2247.0
11	2316.5
12	2386.0
13	2723.0
14	3060.0
15	3193.0
16	3326.0
17	3372.0
18	3418.0
19	3536.0
20	3654.0
21	4009.0
22	4364.0
23	5134.5
24	5905.0
25	8941.5
26	12715.0
27	13452.0
28	16885.5
29	20319.0
30	23165.5
31	26012.0
32	27985.5
33	29959.0
34	33207.0
35	36455.0
36	40858.5
37	45262.0
38	47291.5
39	49321.0
40	55694.0
41	62067.0
42	70352.0
43	78637.0
44	92066.5
45	105496.0
46	121625.5
47	137755.0
48	134344.0
49	130933.0
50	113693.0
51	96453.0
52	85847.0
53	75241.0
54	69772.0
55	64303.0
56	61308.0
57	58313.0
58	57122.5
59	55932.0
60	53901.5
61	51871.0
62	47793.0
63	43715.0
64	38263.5
65	32812.0
66	29218.0
67	25624.0
68	22286.5
69	18949.0
70	15519.0
71	12089.0
72	10550.5
73	9012.0
74	7122.5
75	3939.0
76	2645.0
77	2106.5
78	1568.0
79	1171.0
80	774.0
81	556.0
82	338.0
83	255.0
84	172.0
85	128.5
86	85.0
87	71.0
88	57.0
89	41.0
90	25.0
91	14.0
92	3.0
93	2.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1340176.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.81843470850914
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.1989055030615	18.38244293048105
2	11.60743723283591	5.993717206581838
3	4.632694845939798	3.588267882130304
4	2.2570266834510857	2.3309158424817915
5	1.3760424388437	1.7763630931711873
6	0.8344249913497553	1.2926128294987178
7	0.6049556412731862	1.0933305408029226
8	0.45604007085361786	0.9419392635038412
9	0.3700531217658498	0.8598773126692419
>10	5.3525336365232095	35.82277510447015
>50	1.1922954219151418	19.926884160743715
>100	0.10881500829446429	4.82965731964046
>500	0.004680215409506065	0.7946466663114614
>1k	0.0038026750202236775	1.9546706916435321
>5k	2.9251346309412907E-4	0.4118991558698642
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	5454	0.40696147371688496	No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT	4203	0.3136155251250582	No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	3277	0.2445201227301489	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	3055	0.22795513425102376	No Hit
TCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	2140	0.15968051957354856	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2010	0.14998030109478158	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1672	0.12475973304998747	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1633	0.12184966750635737	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1478	0.11028402239705828	No Hit
ACTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	1477	0.11020940533183701	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCC	1377	0.10274769880970858	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1377	0.10274769880970858	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.35167022838791323	0.0
2	0.0	0.0	0.0	1.3727301488759684	0.0
3	0.0	0.0	0.0	1.8527417294444908	0.0
4	0.0	0.0	0.0	2.4198314251262523	0.0
5	0.0	0.0	0.0	3.7945762347631953	0.0
6	0.0	0.0	0.0	4.535225224149664	0.0
7	0.0	0.0	0.0	5.3328070342999725	0.0
8	0.0	0.0	0.0	6.641366507085636	0.0
9	0.0	0.0	0.0	7.110782464392736	0.0
10	0.0	0.0	0.0	8.314057258151168	0.0
11	0.0	0.0	0.0	10.092778858896144	0.0
12	0.0	0.0	0.0	11.396040520051098	0.0
13	0.0	0.0	0.0	11.91261446257805	0.0
14	0.0	0.0	0.0	12.113483602153748	0.0
15	0.0	0.0	0.0	12.49179212282566	0.0
16	0.0	0.0	0.0	13.312430606129345	0.0
17	0.0	0.0	0.0	14.265738231396472	0.0
18	0.0	0.0	0.0	15.410140160695311	0.0
19	0.0	0.0	0.0	16.034386528336576	0.0
20	0.0	0.0	0.0	16.64579876075978	0.0
21	7.461706522128437E-5	0.0	0.0	17.376150595145713	0.0
22	2.238511956638531E-4	0.0	0.0	18.119336564749705	0.0
23	2.238511956638531E-4	0.0	0.0	18.845435226418022	0.0
24	5.223194565489906E-4	0.0	0.0	19.433492317426964	0.0
25	8.954047826554124E-4	0.0	0.0	19.97476450854216	0.0
26	8.954047826554124E-4	0.0	0.0	20.455522259762898	0.0
27	8.954047826554124E-4	0.0	0.0	20.915984169243444	0.0
28	8.954047826554124E-4	0.0	0.0	21.393160301333555	0.0
29	8.954047826554124E-4	0.0	0.0	21.882722866250404	0.0
30	8.954047826554124E-4	0.0	0.0	22.444514750301455	0.0
31	8.954047826554124E-4	0.0	0.0	22.95743245663256	0.0
32	9.700218478766968E-4	0.0	0.0	23.456172920571625	0.0
33	9.700218478766968E-4	0.0	0.0	23.92238034407421	0.0
34	9.700218478766968E-4	0.0	0.0	24.420001552034957	0.0
35	0.0010446389130979812	0.0	0.0	24.9442610522797	0.0
36	0.0010446389130979812	0.0	0.0	25.44106147252301	0.0
37	0.0010446389130979812	0.0	0.0	25.91719296570003	0.0
38	0.0011192559783192654	0.0	0.0	26.42914065018326	0.0
39	0.0011192559783192654	0.0	0.0	26.92422487792648	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACGA	20	7.033706E-4	45.0	15
CCGTACG	20	7.033706E-4	45.0	1
ACGCTAG	40	6.8157533E-9	45.0	1
ATCGCGA	20	7.033706E-4	45.0	25
TCGCGAT	20	7.033706E-4	45.0	26
ACGTTGA	30	2.1657725E-6	44.999996	33
GCTACGA	255	0.0	40.588234	10
CCAACGG	45	1.9286745E-8	40.0	2
AAGCTAC	260	0.0	39.80769	8
CGAGACA	40	3.4587902E-7	39.375	22
CGAATAT	265	0.0	39.056602	14
AGTACGG	185	0.0	38.91892	2
ACACGCA	35	6.2490653E-6	38.571426	29
TTTGTCG	35	6.2490653E-6	38.571426	1
TACGAAT	270	0.0	38.333336	12
CGAAAGG	195	0.0	38.07692	2
AATATAC	300	0.0	37.5	16
ACTATAC	30	1.1400232E-4	37.499996	26
ATTAGCG	60	1.5643309E-10	37.499996	1
CGACGTT	30	1.1400232E-4	37.499996	27
>>END_MODULE