FastQCFastQC Report
Sat 18 Jun 2016
SRR3552382_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552382_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences694725
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT37280.5366152074561877No Hit
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC32700.4706898413041131No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC26450.38072618662060526No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20650.29723991507431No Hit
TCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC19750.2842851487998848No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCC16040.23088272337975457No Hit
ACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC12540.18050307675699018No Hit
AGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG8360.12033538450466011No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA8110.1167368383173198No Hit
GCCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG7650.11011551333261363No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6990.10061535139803518No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6980.10047140955054157No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAACC253.889206E-545.0000049
CGCGCCA253.889206E-545.00000438
ACTTACG253.889206E-545.0000041
TCTAGAC253.889206E-545.00000426
ATGAACG253.889206E-545.0000041
CGTAAGA253.889206E-545.00000431
TCGACAG253.889206E-545.0000041
GCGTAAG351.2109922E-745.0000041
TCGCCCC207.0313585E-445.027
CGGCATA207.0313585E-445.03
CCGGACC207.0313585E-445.029
CACGCGA207.0313585E-445.020
ATTCGAT207.0313585E-445.014
TCGATCC207.0313585E-445.012
CGTTCAT207.0313585E-445.017
AGACGAA207.0313585E-445.019
ATGCGTT207.0313585E-445.035
CGTAAAG207.0313585E-445.013
ACCCGTA207.0313585E-445.014
GCTCGAT207.0313585E-445.010