##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552382_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	694725
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.091123825974307	31.0	31.0	34.0	28.0	34.0
2	31.284822051891037	31.0	31.0	34.0	28.0	34.0
3	31.33375220410954	31.0	31.0	34.0	28.0	34.0
4	35.100052538774335	37.0	35.0	37.0	32.0	37.0
5	34.9345525207816	35.0	35.0	37.0	32.0	37.0
6	35.031719025513695	36.0	35.0	37.0	32.0	37.0
7	35.46743819496923	37.0	35.0	37.0	33.0	37.0
8	35.47571629061859	37.0	35.0	37.0	33.0	37.0
9	36.90299327071863	39.0	37.0	39.0	33.0	39.0
10	36.46266508330634	38.0	35.0	39.0	32.0	39.0
11	36.20712800028788	38.0	35.0	39.0	32.0	39.0
12	36.17711468566699	38.0	35.0	39.0	32.0	39.0
13	35.84456439598402	38.0	35.0	39.0	30.0	39.0
14	36.93377811364209	39.0	35.0	40.0	31.0	41.0
15	37.06613264241246	39.0	36.0	40.0	32.0	41.0
16	37.06781964086509	39.0	35.0	40.0	32.0	41.0
17	37.10369858577135	39.0	35.0	40.0	32.0	41.0
18	37.02266652272482	39.0	36.0	40.0	32.0	41.0
19	36.92544819892763	39.0	35.0	40.0	31.0	41.0
20	36.5515635683184	38.0	35.0	40.0	30.0	41.0
21	36.58267947749109	38.0	35.0	40.0	30.0	41.0
22	36.67717154269675	38.0	35.0	40.0	31.0	41.0
23	36.50351290078808	38.0	35.0	40.0	30.0	41.0
24	36.524526971103676	38.0	35.0	40.0	30.0	41.0
25	36.53892979236389	38.0	35.0	40.0	31.0	41.0
26	36.479406959588324	38.0	35.0	40.0	30.0	41.0
27	36.26271978120839	38.0	35.0	40.0	30.0	41.0
28	36.24828385332326	38.0	35.0	40.0	30.0	41.0
29	36.267464104501784	38.0	35.0	40.0	30.0	41.0
30	36.07993378675015	38.0	35.0	40.0	30.0	41.0
31	35.863586311130305	38.0	34.0	40.0	29.0	41.0
32	35.52177480297959	38.0	34.0	40.0	27.0	41.0
33	35.372109827629636	38.0	34.0	40.0	27.0	41.0
34	35.274203461801434	38.0	34.0	40.0	26.0	41.0
35	35.07363057324841	38.0	34.0	40.0	25.0	41.0
36	35.10309978768578	38.0	34.0	40.0	25.0	41.0
37	35.10398934830329	38.0	34.0	40.0	26.0	41.0
38	35.023269639065816	38.0	34.0	40.0	25.0	41.0
39	34.967650509194286	38.0	34.0	40.0	25.0	41.0
40	34.79582712584116	38.0	33.0	40.0	24.0	41.0
41	34.705414372593474	38.0	33.0	40.0	24.0	41.0
42	34.74670697038397	38.0	33.0	40.0	24.0	41.0
43	34.67150887041635	37.0	33.0	40.0	24.0	41.0
44	34.55829141027025	37.0	33.0	40.0	24.0	41.0
45	34.447613084313936	37.0	33.0	40.0	23.0	41.0
46	34.27800784483069	37.0	33.0	40.0	23.0	41.0
47	34.16606283061643	37.0	33.0	40.0	23.0	41.0
48	34.15856202094354	37.0	33.0	40.0	23.0	41.0
49	34.11602432617222	37.0	33.0	40.0	23.0	41.0
50	33.96703875634244	36.0	33.0	40.0	23.0	41.0
51	32.7379984886106	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	14.0
9	43.0
10	62.0
11	44.0
12	30.0
13	14.0
14	35.0
15	57.0
16	113.0
17	320.0
18	671.0
19	1042.0
20	1640.0
21	2496.0
22	3539.0
23	4852.0
24	6661.0
25	8127.0
26	9573.0
27	10979.0
28	12855.0
29	15638.0
30	19132.0
31	23953.0
32	29703.0
33	38883.0
34	55867.0
35	63509.0
36	68517.0
37	88642.0
38	120592.0
39	107104.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.79668214041527	20.852855446399655	25.309870812191875	12.0405916009932
2	31.848861060131707	25.468494728129837	24.64860196480622	18.034042246932238
3	30.394616574903736	25.881032063046526	27.0804994782108	16.643851883838927
4	28.30400518190651	27.159091726942314	26.062110907193492	18.474792183957682
5	23.65223649645543	30.621324984706177	25.433804742883876	20.292633775954513
6	24.22800388642988	35.69325992299111	25.278779373133254	14.799956817445754
7	76.54208499766094	7.921983518658463	10.105149519594084	5.430781964086509
8	75.66188060023751	7.068984130411314	10.53092950448019	6.738205764870991
9	69.15211054733888	9.702256288459463	13.556443196948434	7.58918996725323
10	38.20893159163698	26.6771744215337	19.293677354348844	15.820216632480477
11	27.900248299686925	27.740328907121526	24.927273381553853	19.4321494116377
12	25.799273093670156	23.759041347295693	31.413436971463526	19.02824858757062
13	23.42653567958545	26.755766670265213	31.67886573824175	18.138831911907587
14	18.847889452661125	30.768289610997158	28.883515059915794	21.500305876425923
15	17.520169851380043	27.03530173809781	35.38148188131995	20.063046529202204
16	18.55842239735147	27.61034941883479	31.978264781028464	21.852963402785274
17	19.060635503256684	26.993414660477168	28.199935226168627	25.74601461009752
18	20.206700493000827	26.833927093454246	31.257404008780455	21.701968404764475
19	21.18147468422757	28.124509698081972	29.159739465256035	21.534276152434416
20	23.01183921695635	28.337255748677535	28.580661412789233	20.070243621576882
21	21.092662564324012	30.404260678685812	29.18780812551729	19.315268631472886
22	19.869588686170786	27.837633596027207	26.338047428838752	25.95473028896326
23	19.583864118895967	29.12202670121271	27.838353305264675	23.455755874626654
24	21.132966281622227	26.878117240634786	28.276656230882725	23.712260246860268
25	18.66321206232682	28.803915218251824	27.085105617330598	25.447767102090758
26	19.112886393896865	28.726618446147757	28.011659289646985	24.148835870308396
27	22.162870200439023	28.080175609053942	26.88099607758466	22.87595811292238
28	18.008420598078377	27.804958796646158	28.753679513476555	25.43294109179891
29	19.592644571593077	26.569361977760984	27.69282809744863	26.145165353197307
30	22.57425600057577	28.267300010795637	25.46460829824751	23.693835690381086
31	21.35118212242254	30.446291698153942	22.780524668034115	25.422001511389396
32	20.550865450358057	29.656050955414017	25.11209471373565	24.68098888049228
33	21.951851452013386	29.300226708409806	24.551585159595525	24.196336679981286
34	18.465004138328116	28.226420526107454	27.01946813487351	26.28910720069092
35	19.992658965777828	27.378315160675086	26.24175033286552	26.387275540681564
36	22.02123142250531	29.089064018136675	26.308395408255063	22.581309151102953
37	21.462017344992624	28.11990355896218	27.55968188851704	22.858397207528157
38	19.400050379646622	30.165317211846414	27.40163373996905	23.03299866853791
39	21.135701176724602	27.266616287020046	29.25992299111159	22.33775954514376
40	21.38155385224369	29.082730576846956	28.217352189715356	21.318363381193997
41	19.374428730792758	28.53661520745619	25.993738529634026	26.095217532117026
42	21.747022203029974	24.562380798157545	28.82565043722336	24.864946561589118
43	23.408399006801254	21.853395228327756	30.263053726294576	24.475152038576415
44	20.2929216596495	24.54597142754327	27.94659757457987	27.214509338227355
45	21.631436899492602	23.563856202094353	27.94832487674979	26.85638202166325
46	22.2715462952967	26.914534528050666	27.62704667314405	23.18687250350858
47	18.0934902299471	27.070135665191263	31.319011119507717	23.51736298535392
48	19.19147864262838	25.10935981863327	30.85048040591601	24.84868113282234
49	21.40890280326748	22.712871999712117	32.4529849940624	23.425240202958005
50	19.74939724351362	22.79866134081831	31.0445140163374	26.40742739933067
51	18.87638993846486	22.60621109071935	27.343625175429125	31.173773795386662
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	583.0
1	829.5
2	1076.0
3	2491.5
4	3907.0
5	2969.0
6	2031.0
7	2136.5
8	2242.0
9	2335.0
10	2428.0
11	2621.0
12	2814.0
13	3015.5
14	3217.0
15	3254.0
16	3291.0
17	3305.0
18	3319.0
19	3264.5
20	3210.0
21	3244.5
22	3279.0
23	3722.5
24	4166.0
25	4970.5
26	6333.0
27	6891.0
28	8018.0
29	9145.0
30	10300.0
31	11455.0
32	12370.5
33	13286.0
34	14030.0
35	14774.0
36	16074.0
37	17374.0
38	19226.5
39	21079.0
40	24549.0
41	28019.0
42	35291.5
43	42564.0
44	52472.0
45	62380.0
46	74467.5
47	86555.0
48	83126.5
49	79698.0
50	68009.0
51	56320.0
52	48487.0
53	40654.0
54	36073.0
55	31492.0
56	29059.0
57	26626.0
58	24889.5
59	23153.0
60	22216.5
61	21280.0
62	19868.5
63	18457.0
64	15637.0
65	12817.0
66	10998.0
67	9179.0
68	7735.0
69	6291.0
70	5557.5
71	4824.0
72	3906.0
73	2988.0
74	2809.5
75	2114.5
76	1598.0
77	1224.0
78	850.0
79	698.5
80	547.0
81	365.5
82	184.0
83	168.5
84	153.0
85	104.0
86	55.0
87	40.5
88	26.0
89	17.5
90	9.0
91	11.0
92	13.0
93	15.5
94	18.0
95	10.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	694725.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.849264654254824
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.22738279711051	24.786930602378206
2	11.15029625693955	7.548586579489876
3	4.443842105635361	4.5126236254611705
4	2.2324500585824447	3.0226717144145536
5	1.385768229922109	2.3453617782045857
6	0.8813621500538816	1.7900076404050134
7	0.6563569937637934	1.555204111270721
8	0.49385613700660524	1.3373333666131608
9	0.40016300161952084	1.2190701011994147
>10	4.672370907022872	35.772641974183706
>50	0.3797685378250489	8.237294581786585
>100	0.06651313370977477	4.004478473478323
>500	0.0068658718668154605	1.4651664682061678
>1k	0.0030038189417317634	2.402628982908518
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT	3728	0.5366152074561877	No Hit
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	3270	0.4706898413041131	No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	2645	0.38072618662060526	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2065	0.29723991507431	No Hit
TCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	1975	0.2842851487998848	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCC	1604	0.23088272337975457	No Hit
ACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	1254	0.18050307675699018	No Hit
AGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG	836	0.12033538450466011	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	811	0.1167368383173198	No Hit
GCCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG	765	0.11011551333261363	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	699	0.10061535139803518	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	698	0.10047140955054157	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6152074561877001	0.0
2	0.0	0.0	0.0	2.101119147864263	0.0
3	0.0	0.0	0.0	2.978588650185325	0.0
4	0.0	0.0	0.0	4.019863974954118	0.0
5	0.0	0.0	0.0	6.222893950843859	0.0
6	0.0	0.0	0.0	7.440066213249847	0.0
7	0.0	0.0	0.0	8.701428622836374	0.0
8	0.0	0.0	0.0	10.976861348015401	0.0
9	0.0	0.0	0.0	11.846701932419302	0.0
10	0.0	0.0	0.0	13.756090539422074	0.0
11	0.0	0.0	0.0	16.74677030479686	0.0
12	0.0	0.0	0.0	19.012414984346325	0.0
13	0.0	0.0	0.0	19.871172046493218	0.0
14	0.0	0.0	0.0	20.177912123502104	0.0
15	0.0	0.0	0.0	20.746194537406886	0.0
16	0.0	0.0	0.0	21.980064054122135	0.0
17	0.0	0.0	0.0	23.40148979812156	0.0
18	0.0	0.0	0.0	25.2754687106409	0.0
19	0.0	0.0	0.0	26.164597502608945	0.0
20	0.0	0.0	0.0	27.04652920220231	0.0
21	4.3182554248083775E-4	0.0	0.0	28.0473568678254	0.0
22	8.636510849616755E-4	0.0	0.0	28.99175932923099	0.0
23	0.0011515347799489007	0.0	0.0	29.91385080427507	0.0
24	0.004174313577314765	0.0	0.0	30.600165533124617	0.0
25	0.004894022814782828	0.0	0.0	31.218107884414696	0.0
26	0.00503796466227644	0.0	0.0	31.795314692864082	0.0
27	0.00503796466227644	0.0	0.0	32.34186188779733	0.0
28	0.00503796466227644	0.0	0.0	32.92770520709633	0.0
29	0.00503796466227644	0.0	0.0	33.516571305192706	0.0
30	0.00503796466227644	0.0	0.0	34.171362769441146	0.0
31	0.005181906509770053	0.0	0.0	34.77908524955918	0.0
32	0.005181906509770053	0.0	0.0	35.402065565511535	0.0
33	0.005613732052250891	0.0	0.0	35.978264781028464	0.0
34	0.005901615747238116	0.0	0.0	36.54208499766094	0.0
35	0.006045557594731728	0.0	0.0	37.14160279247184	0.0
36	0.006045557594731728	0.0	0.0	37.701104753679516	0.0
37	0.006333441289718953	0.0	0.0	38.29155421209831	0.0
38	0.0067652668321997915	0.0	0.0	38.86156392817302	0.0
39	0.0067652668321997915	0.0	0.0	39.481953290870486	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACAACC	25	3.889206E-5	45.000004	9
CGCGCCA	25	3.889206E-5	45.000004	38
ACTTACG	25	3.889206E-5	45.000004	1
TCTAGAC	25	3.889206E-5	45.000004	26
ATGAACG	25	3.889206E-5	45.000004	1
CGTAAGA	25	3.889206E-5	45.000004	31
TCGACAG	25	3.889206E-5	45.000004	1
GCGTAAG	35	1.2109922E-7	45.000004	1
TCGCCCC	20	7.0313585E-4	45.0	27
CGGCATA	20	7.0313585E-4	45.0	3
CCGGACC	20	7.0313585E-4	45.0	29
CACGCGA	20	7.0313585E-4	45.0	20
ATTCGAT	20	7.0313585E-4	45.0	14
TCGATCC	20	7.0313585E-4	45.0	12
CGTTCAT	20	7.0313585E-4	45.0	17
AGACGAA	20	7.0313585E-4	45.0	19
ATGCGTT	20	7.0313585E-4	45.0	35
CGTAAAG	20	7.0313585E-4	45.0	13
ACCCGTA	20	7.0313585E-4	45.0	14
GCTCGAT	20	7.0313585E-4	45.0	10
>>END_MODULE