FastQCFastQC Report
Sat 18 Jun 2016
SRR3552381_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552381_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2402720
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC42490.17684124658720118No Hit
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC41990.1747602716920823TruSeq Adapter, Index 23 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT30340.12627355663581274No Hit
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC27960.11636811613504694TruSeq Adapter, Index 23 (95% over 22bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG24700.10280015981887194No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG24160.10055270693214358No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG502.1827873E-1145.01
GTACGAC207.0348254E-445.035
GTACGAG1300.041.538461
TACGAAT4350.040.34482612
GTATGCG1400.040.178571
CGAATAT4250.039.70588314
ACGGGTA3350.039.6268655
CGTTAGG3450.039.1304322
CACGACC8900.038.1741627
TGTTACG1300.038.076921
ACACGAC8950.037.96089626
TCGTAAG950.037.8947371
CCCGTTA301.1402923E-437.50000441
ACGTAAG601.5643309E-1037.5000041
ATGTACG900.037.51
ATAACGG900.037.52
CGTGTAC7150.037.4475518
CCCTCGT7350.037.34694314
GCGAGAC9600.037.03125421
AACACGT8750.037.02857241
Produced by FastQC (version 0.11.5)