##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552381_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2402720
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.413051874542184	31.0	31.0	34.0	30.0	34.0
2	31.61878496037824	31.0	31.0	34.0	30.0	34.0
3	31.701872045015648	31.0	31.0	34.0	30.0	34.0
4	35.40084695678231	37.0	35.0	37.0	33.0	37.0
5	35.20272441233269	37.0	35.0	37.0	33.0	37.0
6	35.2841050975561	37.0	35.0	37.0	32.0	37.0
7	35.640535726177	37.0	35.0	37.0	35.0	37.0
8	35.65679438303256	37.0	35.0	37.0	35.0	37.0
9	37.14912599054405	39.0	37.0	39.0	34.0	39.0
10	36.656796880202435	38.0	37.0	39.0	32.0	39.0
11	36.38517763201705	38.0	35.0	39.0	32.0	39.0
12	36.33666844243191	38.0	35.0	39.0	32.0	39.0
13	36.09246895185456	38.0	35.0	39.0	31.0	39.0
14	37.320170473463406	39.0	36.0	41.0	32.0	41.0
15	37.43214024106013	39.0	36.0	41.0	32.0	41.0
16	37.40018229340081	39.0	36.0	41.0	32.0	41.0
17	37.3965892821469	39.0	36.0	41.0	32.0	41.0
18	37.3681527602051	39.0	36.0	41.0	32.0	41.0
19	37.34774339082373	39.0	36.0	41.0	32.0	41.0
20	36.953406972098286	39.0	35.0	41.0	31.0	41.0
21	37.032057834454285	39.0	35.0	40.0	31.0	41.0
22	37.11142829792902	39.0	35.0	40.0	31.0	41.0
23	36.963440600652596	39.0	35.0	40.0	31.0	41.0
24	36.999841429712994	39.0	35.0	40.0	31.0	41.0
25	37.002095957914364	39.0	35.0	40.0	31.0	41.0
26	36.931826430045945	39.0	35.0	40.0	31.0	41.0
27	36.71279799560498	39.0	35.0	40.0	31.0	41.0
28	36.658469151628154	39.0	35.0	40.0	30.0	41.0
29	36.69440300992209	39.0	35.0	40.0	30.0	41.0
30	36.580705616967435	39.0	35.0	40.0	30.0	41.0
31	36.4880535393221	38.0	35.0	40.0	30.0	41.0
32	36.26732245122195	38.0	35.0	40.0	30.0	41.0
33	36.19879136978091	38.0	35.0	40.0	30.0	41.0
34	36.14271117733236	38.0	35.0	40.0	30.0	41.0
35	35.971263401478325	38.0	35.0	40.0	29.0	41.0
36	36.01234475927282	38.0	35.0	40.0	29.0	41.0
37	36.011824099354065	38.0	35.0	40.0	29.0	41.0
38	35.95090439168942	38.0	35.0	40.0	29.0	41.0
39	35.91724670373576	38.0	34.0	40.0	29.0	41.0
40	35.8030099220883	38.0	34.0	40.0	29.0	41.0
41	35.69756401078777	38.0	34.0	40.0	28.0	41.0
42	35.680747652660315	38.0	34.0	40.0	28.0	41.0
43	35.5802440567357	38.0	34.0	40.0	28.0	41.0
44	35.46253995471799	38.0	34.0	40.0	28.0	41.0
45	35.371748268628885	38.0	34.0	40.0	28.0	41.0
46	35.22931843910235	37.0	34.0	40.0	27.0	41.0
47	35.112788006925484	37.0	34.0	40.0	27.0	41.0
48	35.045999950056604	37.0	34.0	40.0	27.0	41.0
49	34.94029266830925	37.0	33.0	40.0	27.0	41.0
50	34.814732886062465	36.0	33.0	40.0	27.0	41.0
51	33.53519011786642	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	57.0
9	149.0
10	197.0
11	138.0
12	88.0
13	60.0
14	71.0
15	126.0
16	243.0
17	447.0
18	923.0
19	1664.0
20	2818.0
21	4387.0
22	6587.0
23	9354.0
24	13428.0
25	18005.0
26	23509.0
27	30078.0
28	37673.0
29	47516.0
30	58976.0
31	75040.0
32	95422.0
33	125253.0
34	186146.0
35	207639.0
36	235750.0
37	314998.0
38	444180.0
39	461734.0
40	60.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.68977658653526	19.94027602051009	25.722056669108344	10.647890723846308
2	30.670073916228276	24.655432176866217	25.97127422254778	18.703219684357727
3	32.95456815608976	24.23761403742425	25.626872877405603	17.180944929080376
4	29.926874542185523	26.358959845508423	24.217844775920625	19.49632083638543
5	26.03869780914963	30.735583005926614	23.2807817806486	19.944937404275155
6	26.729414996337486	36.35492275421189	22.06307851102084	14.85258373842978
7	83.4714823200373	6.6146284211227275	6.020343610574682	3.8935456482652993
8	85.02592894719318	5.510088566291536	5.6914663381500965	3.7725161483651863
9	78.82304221881867	7.630185789438635	7.847897382965972	5.69887460877672
10	42.59776420057268	26.225777452220818	15.707906039821534	15.468552307384964
11	31.962983618565627	26.970766464673368	21.109575814077377	19.956674102683625
12	27.352250782446564	24.369131650795765	27.74284977026037	20.535767796497304
13	24.60224245854698	25.592911367117267	28.44526203635879	21.35958413797696
14	21.522524472264767	27.455508756742358	28.187887061330493	22.834079709662383
15	22.346174335752814	26.264899780249053	29.78940534061397	21.599520543384166
16	24.168484051408402	25.65113704468269	28.37471698741426	21.80566191649464
17	24.16020177132583	25.613637877072655	26.534885463141773	23.691274888459745
18	25.290212758873277	24.77700273023906	27.41209962043018	22.52068489045748
19	25.866268229340083	26.01801291869215	25.67157221815276	22.44414663381501
20	26.92261270560032	25.67011553572618	26.251206965439167	21.156064793234332
21	26.231687420922956	26.817273756409403	25.812412599054408	21.138626223613237
22	24.680903309582472	25.404957714590132	25.390390890324298	24.523748085503097
23	24.54376706399414	26.316050143171072	25.910592994606112	23.229589798228677
24	23.514974695345277	25.82577245788107	27.5682975960578	23.090955250715854
25	24.01370113870946	26.775695878004928	25.974312445894654	23.236290537390957
26	23.760363254977694	27.27175867350336	25.835927615369247	23.131950456149696
27	24.356687420922952	26.574840181128057	26.57159386029167	22.496878537657324
28	22.198050542718253	27.82883565292668	27.12575747486182	22.84735632949324
29	24.136561896517282	26.177582073649862	25.67190517413598	24.013950855696876
30	24.622511154025435	25.935689551841246	25.693339215555707	23.74846007857761
31	24.787324365718852	27.85463974162616	23.97570253712459	23.3823333555304
32	24.464731637477524	27.25173969501232	24.439177265765466	23.84435140174469
33	23.653109808883265	27.43028734101352	24.057235133515352	24.859367716587865
34	22.755710195112204	27.113854298461742	26.507583072517814	23.622852433908236
35	24.394810881001533	25.51887028034894	26.334445961243922	23.751872877405606
36	23.964714989678367	29.2331191316508	24.389400346274222	22.412765532396616
37	23.553014916428047	28.621395751481653	25.743407471532265	22.082181860558034
38	22.482478191383098	28.300634281148035	25.550084903775723	23.666802623693147
39	23.3140357594726	25.894111673436772	25.816741026836254	24.975111540254378
40	23.79848671505627	25.027344010121862	28.54040420856363	22.633765066258242
41	21.35904308450423	26.292868082839448	27.566050143171072	24.78203868948525
42	22.82758706798961	26.448691482985947	26.78451921156023	23.939202237464208
43	23.846723713125126	25.303322900712526	26.63947526137045	24.210478124791905
44	23.114595125524406	25.612097955650263	25.70282846107745	25.570478457747885
45	23.520926283545315	24.785451488313246	25.8876606512619	25.80596157687954
46	23.10327462209496	25.683808350536058	26.853524339082373	24.359392688286608
47	22.559765598987813	25.885787773856293	28.618940201105413	22.935506426050477
48	22.10415695545049	25.825356262902044	27.952320703203036	24.118166078444432
49	22.67946327495505	26.31322001731371	27.674801891189986	23.332514816541252
50	21.384847173203703	25.648764733302258	27.873909569154957	25.092478524339086
51	21.54995172138243	24.872103282945996	26.600685889325433	26.97725910634614
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	539.0
1	1138.5
2	1738.0
3	3237.0
4	4736.0
5	3636.0
6	2536.0
7	2484.0
8	2432.0
9	2597.0
10	2762.0
11	2877.5
12	2993.0
13	3237.0
14	3481.0
15	3848.0
16	4215.0
17	4257.0
18	4299.0
19	4659.0
20	5019.0
21	5980.0
22	6941.0
23	7830.0
24	8719.0
25	12466.0
26	18862.0
27	21511.0
28	27669.0
29	33827.0
30	39902.5
31	45978.0
32	50808.0
33	55638.0
34	60623.0
35	65608.0
36	70690.5
37	75773.0
38	79151.0
39	82529.0
40	91050.0
41	99571.0
42	106146.0
43	112721.0
44	125505.0
45	138289.0
46	152799.5
47	167310.0
48	182071.0
49	196832.0
50	196282.0
51	195732.0
52	182944.5
53	170157.0
54	157913.0
55	145669.0
56	136688.0
57	127707.0
58	125316.5
59	122926.0
60	118925.0
61	114924.0
62	108253.0
63	101582.0
64	88164.5
65	74747.0
66	65530.0
67	56313.0
68	49188.0
69	42063.0
70	36639.5
71	31216.0
72	26857.5
73	22499.0
74	18980.0
75	12297.5
76	9134.0
77	7233.5
78	5333.0
79	4133.0
80	2933.0
81	2118.0
82	1303.0
83	877.0
84	451.0
85	336.0
86	221.0
87	142.0
88	63.0
89	51.5
90	40.0
91	30.0
92	20.0
93	15.5
94	11.0
95	7.5
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2402720.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.865672455998574
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.69280704273598	15.457457637915741
2	11.541887163576815	5.047422484857302
3	4.584693763713394	3.007422364452492
4	2.2696596228484562	1.9851053559923841
5	1.3219431493986786	1.445258795510135
6	0.8507889742818141	1.1161843824492688
7	0.586461015137319	0.8976355125643527
8	0.436993135152321	0.7644119007008455
9	0.3365359676983872	0.6622726715419905
>10	5.069573464272277	29.61219366777343
>50	2.0979984990311333	30.966722072117086
>100	0.20111832018999296	6.391043601078791
>500	0.005646052590626008	0.8613309261899762
>1k	0.003893829372845523	1.7855386268564315
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	4249	0.17684124658720118	No Hit
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	4199	0.1747602716920823	TruSeq Adapter, Index 23 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3034	0.12627355663581274	No Hit
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	2796	0.11636811613504694	TruSeq Adapter, Index 23 (95% over 22bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2470	0.10280015981887194	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2416	0.10055270693214358	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.16194979023773E-5	0.0	0.0	0.10854365052940002	0.0
2	4.16194979023773E-5	0.0	0.0	0.5812579077046015	0.0
3	4.16194979023773E-5	0.0	0.0	0.7762452553772391	0.0
4	4.16194979023773E-5	0.0	0.0	1.007774522208164	0.0
5	4.16194979023773E-5	0.0	0.0	1.7094792568422454	0.0
6	4.16194979023773E-5	0.0	0.0	2.133998135446494	0.0
7	4.16194979023773E-5	0.0	0.0	2.5743740427515482	0.0
8	4.16194979023773E-5	0.0	0.0	3.2608460411533597	0.0
9	4.16194979023773E-5	0.0	0.0	3.508898248651528	0.0
10	4.16194979023773E-5	0.0	0.0	4.20727342345342	0.0
11	4.16194979023773E-5	0.0	0.0	5.220500099886795	0.0
12	4.16194979023773E-5	0.0	0.0	5.964407005393887	0.0
13	4.16194979023773E-5	0.0	0.0	6.266897516148365	0.0
14	4.16194979023773E-5	0.0	0.0	6.387177865086236	0.0
15	4.16194979023773E-5	0.0	0.0	6.64093194379703	0.0
16	4.16194979023773E-5	0.0	0.0	7.201879536525271	0.0
17	4.16194979023773E-5	0.0	0.0	7.877280748485051	0.0
18	4.16194979023773E-5	0.0	0.0	8.582564759938736	0.0
19	4.16194979023773E-5	0.0	0.0	9.007458214024107	0.0
20	4.16194979023773E-5	0.0	0.0	9.411874875141507	0.0
21	4.16194979023773E-5	0.0	0.0	9.922587733901578	0.0
22	7.491509622427915E-4	0.0	0.0	10.477875074915096	0.0
23	7.491509622427915E-4	0.0	0.0	11.028625890657255	0.0
24	7.491509622427915E-4	0.0	0.0	11.503171405740162	0.0
25	8.740094559499235E-4	0.0	0.0	11.930187454218553	0.0
26	8.740094559499235E-4	0.0	0.0	12.31845575014983	0.0
27	8.740094559499235E-4	0.0	0.0	12.700064926416728	0.0
28	8.740094559499235E-4	0.0	0.0	13.102442232136912	0.0
29	8.740094559499235E-4	0.0	0.0	13.520260371578877	0.0
30	8.740094559499235E-4	0.0	0.0	13.995763135113538	0.0
31	9.57248451754678E-4	0.0	0.0	14.43855297329693	0.0
32	9.57248451754678E-4	0.0	0.0	14.885962575747486	0.0
33	9.988679496570553E-4	0.0	0.0	15.32721249250849	0.0
34	9.988679496570553E-4	0.0	0.0	15.760096890191116	0.0
35	9.988679496570553E-4	0.0	0.0	16.25104048744756	0.0
36	9.988679496570553E-4	0.0	0.0	16.69520376906173	0.0
37	0.00108210694546181	0.0	0.0	17.13545481787308	0.0
38	0.00108210694546181	0.0	0.0	17.589565159485915	0.0
39	0.00108210694546181	0.0	0.0	18.038597922354665	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATACG	50	2.1827873E-11	45.0	1
GTACGAC	20	7.0348254E-4	45.0	35
GTACGAG	130	0.0	41.53846	1
TACGAAT	435	0.0	40.344826	12
GTATGCG	140	0.0	40.17857	1
CGAATAT	425	0.0	39.705883	14
ACGGGTA	335	0.0	39.626865	5
CGTTAGG	345	0.0	39.130432	2
CACGACC	890	0.0	38.17416	27
TGTTACG	130	0.0	38.07692	1
ACACGAC	895	0.0	37.960896	26
TCGTAAG	95	0.0	37.894737	1
CCCGTTA	30	1.1402923E-4	37.500004	41
ACGTAAG	60	1.5643309E-10	37.500004	1
ATGTACG	90	0.0	37.5	1
ATAACGG	90	0.0	37.5	2
CGTGTAC	715	0.0	37.44755	18
CCCTCGT	735	0.0	37.346943	14
GCGAGAC	960	0.0	37.031254	21
AACACGT	875	0.0	37.028572	41
>>END_MODULE