##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552375_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	867874
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.362935172617224	31.0	31.0	34.0	30.0	34.0
2	31.569830413170575	31.0	31.0	34.0	30.0	34.0
3	31.64686002806859	31.0	31.0	34.0	30.0	34.0
4	35.35675685641003	37.0	35.0	37.0	33.0	37.0
5	35.16976312229655	37.0	35.0	37.0	33.0	37.0
6	35.26443354680518	37.0	35.0	37.0	32.0	37.0
7	35.63356201476251	37.0	35.0	37.0	35.0	37.0
8	35.67374526717012	37.0	35.0	37.0	35.0	37.0
9	37.17224504939657	39.0	37.0	39.0	34.0	39.0
10	36.70162373800805	38.0	37.0	39.0	32.0	39.0
11	36.39809234981115	38.0	35.0	39.0	32.0	39.0
12	36.305886568787635	38.0	35.0	39.0	32.0	39.0
13	35.98986027925713	38.0	35.0	39.0	31.0	39.0
14	37.14481825702809	39.0	36.0	40.0	32.0	41.0
15	37.27126979261966	39.0	36.0	41.0	32.0	41.0
16	37.288949778424055	39.0	36.0	41.0	32.0	41.0
17	37.295068178099584	39.0	36.0	41.0	32.0	41.0
18	37.28186580079597	39.0	36.0	41.0	32.0	41.0
19	37.22609848895116	39.0	35.0	40.0	32.0	41.0
20	36.86243510002604	39.0	35.0	40.0	31.0	41.0
21	36.941628623509864	39.0	35.0	40.0	31.0	41.0
22	37.04136660390794	39.0	35.0	40.0	31.0	41.0
23	36.88781090342607	39.0	35.0	40.0	31.0	41.0
24	36.928190036802576	39.0	35.0	40.0	31.0	41.0
25	36.93355602310935	39.0	35.0	40.0	31.0	41.0
26	36.8752042347161	39.0	35.0	40.0	31.0	41.0
27	36.667638389904525	39.0	35.0	40.0	31.0	41.0
28	36.63169423211203	39.0	35.0	40.0	31.0	41.0
29	36.65602956189493	38.0	35.0	40.0	31.0	41.0
30	36.50760824728013	38.0	35.0	40.0	30.0	41.0
31	36.37034984341045	38.0	35.0	40.0	30.0	41.0
32	36.09715926505461	38.0	35.0	40.0	30.0	41.0
33	35.98518448530547	38.0	35.0	40.0	30.0	41.0
34	35.8605788397855	38.0	35.0	40.0	29.0	41.0
35	35.7187783019194	38.0	34.0	40.0	29.0	41.0
36	35.76071641736012	38.0	34.0	40.0	29.0	41.0
37	35.77349822670111	38.0	34.0	40.0	29.0	41.0
38	35.689820181270555	38.0	34.0	40.0	29.0	41.0
39	35.655588253594416	38.0	34.0	40.0	29.0	41.0
40	35.49232492274224	38.0	34.0	40.0	28.0	41.0
41	35.374762926415585	38.0	34.0	40.0	28.0	41.0
42	35.41973834911519	38.0	34.0	40.0	28.0	41.0
43	35.34849759296856	38.0	34.0	40.0	28.0	41.0
44	35.23792854723151	37.0	34.0	40.0	27.0	41.0
45	35.173280914049734	37.0	34.0	40.0	27.0	41.0
46	35.04625901916638	37.0	34.0	40.0	27.0	41.0
47	34.93015460769651	37.0	34.0	40.0	27.0	41.0
48	34.863194426840764	37.0	34.0	40.0	27.0	41.0
49	34.78926664469727	37.0	33.0	40.0	27.0	41.0
50	34.64503142161189	37.0	33.0	40.0	26.0	41.0
51	33.27029499673916	35.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	17.0
9	54.0
10	68.0
11	46.0
12	31.0
13	10.0
14	21.0
15	43.0
16	94.0
17	188.0
18	390.0
19	746.0
20	1164.0
21	1772.0
22	2745.0
23	3801.0
24	5359.0
25	6965.0
26	8689.0
27	10662.0
28	13267.0
29	16887.0
30	21570.0
31	27421.0
32	35774.0
33	47734.0
34	72661.0
35	80564.0
36	85540.0
37	113748.0
38	159270.0
39	150549.0
40	20.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.552836010757325	20.949354399371337	25.19570813274738	11.30210145712396
2	31.126638198632524	24.85199464438386	25.8534072918419	18.167959865141714
3	32.03310618822548	24.85326210947672	25.907677842636147	17.205953859661655
4	29.106183616515764	26.32905237396212	25.457266838273757	19.10749717124836
5	25.39308701493535	29.99421575021259	24.10926009996843	20.503437134883633
6	25.703270290387774	35.28380847911102	22.99285380135826	16.02006742914294
7	81.46747108451227	6.041545201261934	7.481846443147278	5.0091372710785205
8	82.17817332930817	4.991623207977195	7.3556760543581206	5.474527408356512
9	76.37329842811283	7.399576436210786	9.889223550884115	6.337901584792262
10	42.15853914277879	25.70280939399037	16.450659888416983	15.687991574813855
11	33.136722611807706	25.949965087097898	22.018518817247664	18.894793483846733
12	29.982578116178153	22.676102752242837	27.798620537082574	19.542698594496436
13	25.625494023325967	25.238571497705887	28.367481915577606	20.76845256339054
14	20.28681582810408	28.882187967377753	27.854158552969672	22.976837651548497
15	19.598351834482884	26.459255606228556	33.36302274293273	20.57936981635583
16	22.88673240585615	25.630218211399352	30.283543463682516	21.199505919061984
17	21.506578143831938	25.633329262081823	27.24335560231094	25.6167369917753
18	24.090017675376842	24.597234160719182	28.95593139096228	22.356816772941695
19	24.761543726393462	27.35120535930331	26.126488407303366	21.760762506999864
20	27.19438536008683	26.393693093697934	25.97761887094209	20.434302675273138
21	24.63295363151794	28.03609740584463	26.344492403275126	20.986456559362303
22	22.847095315679464	26.369956929231662	25.306092819925475	25.476854935163402
23	23.15958307311891	27.38876841569168	26.196544659708664	23.255103851480747
24	23.912226890078514	24.373814632077927	27.870174702779437	23.84378377506412
25	22.392881916038505	26.48748551056951	25.480542106342625	25.639090467049364
26	21.46198641738317	27.271931178949938	26.94342727170073	24.32265513196616
27	22.154944150879043	27.424718334689135	26.56433998483651	23.85599752959531
28	19.571619843433492	29.355298119312252	26.705604730640626	24.367477306613633
29	23.016820414023233	26.804121335585577	26.652140748541843	23.526917501849347
30	24.198904449263374	25.277517243286468	26.754459748765374	23.769118558684784
31	24.521992823843092	28.590555771920812	23.451791389072607	23.435660015163492
32	25.626300592021423	26.792944597948548	25.42707812424384	22.153676685786188
33	25.442863825854907	26.479189375416247	24.682384770139446	23.395562028589403
34	23.431742395785562	25.657411098846143	28.17286841177406	22.737978093594233
35	23.383348274058218	24.262277703906328	27.802307708261797	24.552066313773658
36	23.536250653896765	28.749219356726897	25.704883427778686	22.009646561597652
37	25.768717578819043	28.04301085180568	25.203543371503233	20.98472819787204
38	24.700359729638173	27.68097673164538	25.63171612469091	21.986947414025536
39	27.26547862938629	23.365373314559488	26.85919845507528	22.509949600978942
40	26.338155077810832	21.997778479364516	29.782433855605767	21.88163258721888
41	21.83392981008764	24.008784685334508	28.7705358151068	25.38674968947105
42	24.49779576297942	23.605961234004013	27.78986350553191	24.10637949748466
43	24.56877380817953	23.9101528562902	27.29140405174023	24.22966928379004
44	23.629812622569634	24.665907723932275	26.586578236011217	25.11770141748687
45	24.68307611473555	22.667345720692175	26.768517088886174	25.8810610756861
46	23.612644231766364	24.40734484498902	27.882388457310626	24.097622465933995
47	22.0099922338957	24.721791412117426	29.59715350384964	23.671062850137233
48	22.48125880024059	23.03709985550898	29.623655046700325	24.857986297550106
49	24.728704858078476	22.50430362011075	29.51511394511185	23.25187757669892
50	21.848678494804545	22.97891168533681	28.85015566775822	26.32225415210042
51	20.770411373079504	22.408552393550217	27.157628872393918	29.66340736097636
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	325.0
1	569.0
2	813.0
3	1407.0
4	2001.0
5	1582.5
6	1164.0
7	1236.0
8	1308.0
9	1393.5
10	1479.0
11	1576.0
12	1673.0
13	1831.5
14	1990.0
15	2131.5
16	2273.0
17	2496.0
18	2719.0
19	2527.5
20	2336.0
21	2405.5
22	2475.0
23	2899.5
24	3324.0
25	3793.0
26	5031.5
27	5801.0
28	7272.0
29	8743.0
30	10517.5
31	12292.0
32	13617.5
33	14943.0
34	16336.5
35	17730.0
36	19821.5
37	21913.0
38	23804.0
39	25695.0
40	28587.0
41	31479.0
42	35975.0
43	40471.0
44	46115.5
45	51760.0
46	59800.0
47	67840.0
48	78142.5
49	88445.0
50	89818.0
51	91191.0
52	80239.5
53	69288.0
54	61903.0
55	54518.0
56	50293.5
57	46069.0
58	44630.0
59	43191.0
60	41928.0
61	40665.0
62	37778.0
63	34891.0
64	29981.0
65	25071.0
66	20407.0
67	15743.0
68	13285.0
69	10827.0
70	9203.0
71	7579.0
72	6554.0
73	5529.0
74	4470.5
75	2774.5
76	2137.0
77	1652.5
78	1168.0
79	973.5
80	779.0
81	548.5
82	318.0
83	213.5
84	109.0
85	89.5
86	70.0
87	47.0
88	24.0
89	24.0
90	24.0
91	16.0
92	8.0
93	7.0
94	6.0
95	3.0
96	0.0
97	0.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	867874.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.42172404375185
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.29941766997982	21.271735152101027
2	11.125547708238754	6.54665589014793
3	4.349037708818186	3.838685619741586
4	2.067017613582781	2.4326088728162834
5	1.2549759975425958	1.8461778740615227
6	0.8207284932375429	1.4488348345727549
7	0.6173942553048017	1.271536238404289
8	0.4567710901157855	1.0751194371640282
9	0.38447612024203537	1.0180755280056168
>10	5.983278108110128	43.653539014357044
>50	0.5763074565899852	10.383628528589925
>100	0.05989155191386896	3.2232659102463828
>500	0.003569695147184242	0.7270173643544753
>1k	0.00158653117652633	1.263119735437053
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	3514	0.40489748511880763	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT	3214	0.37033025531355934	TruSeq Adapter, Index 15 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	2737	0.31536835992321466	TruSeq Adapter, Index 14 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	1359	0.15658955101777447	TruSeq Adapter, Index 14 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	936	0.10784975699237448	TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCC	917	0.10566049910470876	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	872	0.10047541463392151	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.432666493062357	0.0
2	0.0	0.0	0.0	1.5579450473225376	0.0
3	0.0	0.0	0.0	2.069770496638913	0.0
4	0.0	0.0	0.0	2.6858737558677874	0.0
5	0.0	0.0	0.0	4.139656217377177	0.0
6	0.0	0.0	0.0	4.956479857675192	0.0
7	0.0	0.0	0.0	5.8325286850395335	0.0
8	1.1522409935082743E-4	0.0	0.0	7.368235481187361	0.0
9	1.1522409935082743E-4	0.0	0.0	7.894348718823239	0.0
10	1.1522409935082743E-4	0.0	0.0	9.244199042718183	0.0
11	1.1522409935082743E-4	0.0	0.0	11.284587394022635	0.0
12	1.1522409935082743E-4	0.0	0.0	12.783422478378197	0.0
13	1.1522409935082743E-4	0.0	0.0	13.398373496613564	0.0
14	1.1522409935082743E-4	0.0	0.0	13.641957242641213	0.0
15	2.3044819870165486E-4	0.0	0.0	14.083726439552285	0.0
16	2.3044819870165486E-4	0.0	0.0	14.99514906541733	0.0
17	2.3044819870165486E-4	0.0	0.0	16.083555907885245	0.0
18	2.3044819870165486E-4	0.0	0.0	17.347564277763823	0.0
19	2.3044819870165486E-4	0.0	0.0	18.03487603039151	0.0
20	2.3044819870165486E-4	0.0	0.0	18.71181761407762	0.0
21	4.608963974033097E-4	0.0	0.0	19.514699138354185	0.0
22	8.065686954557919E-4	0.0	0.0	20.31193468176256	0.0
23	0.0010370168941574468	0.0	0.0	21.099376176726114	0.0
24	0.0014979132915607565	0.0	0.0	21.697850148754313	0.0
25	0.0016131373909115839	0.0	0.0	22.236177140921377	0.0
26	0.0016131373909115839	0.0	0.0	22.702143398696123	0.0
27	0.0016131373909115839	0.0	0.0	23.196800457209225	0.0
28	0.0016131373909115839	0.0	0.0	23.701136340067798	0.0
29	0.0016131373909115839	0.0	0.0	24.192567123799076	0.0
30	0.0016131373909115839	0.0	0.0	24.764770001175286	0.0
31	0.0016131373909115839	0.0	0.0	25.29480085818909	0.0
32	0.0016131373909115839	0.0	0.0	25.805704514710662	0.0
33	0.0016131373909115839	0.0	0.0	26.307966363780917	0.0
34	0.0018435855896132389	0.0	0.0	26.785339807391395	0.0
35	0.0018435855896132389	0.0	0.0	27.29416943012465	0.0
36	0.0018435855896132389	0.0	0.0	27.81129518801116	0.0
37	0.0018435855896132389	0.0	0.0	28.330955876083394	0.0
38	0.0018435855896132389	0.0	0.0	28.873315711727738	0.0
39	0.0018435855896132389	0.0	0.0	29.45588875804552	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTGCG	55	1.8189894E-12	45.0	1
ATTACAC	25	3.890013E-5	45.0	16
ATTCGCG	20	7.032331E-4	45.0	1
TAACGCG	40	6.8121153E-9	45.0	1
AAAGTCG	35	1.2114651E-7	45.0	1
CTACGCA	75	0.0	45.0	15
CCGACTA	20	7.032331E-4	45.0	44
TCATCGC	20	7.032331E-4	45.0	36
TAGGCCC	20	7.032331E-4	45.0	40
TCGACCC	20	7.032331E-4	45.0	25
TATCGGT	20	7.032331E-4	45.0	30
TGCGTAG	45	3.8380676E-10	45.0	1
TACGTAG	95	0.0	42.63158	1
TACGGTT	110	0.0	40.909092	33
CGTATGG	105	0.0	40.714287	2
GTTTACG	45	1.9274012E-8	40.0	1
ACGCATT	85	0.0	39.705883	17
CGAATAT	125	0.0	39.600002	14
ATCACGC	40	3.4569166E-7	39.375	45
TAGTGCG	80	0.0	39.375	1
>>END_MODULE