##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552374_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	965409
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.31937344690178	31.0	31.0	34.0	30.0	34.0
2	31.515194078364715	31.0	31.0	34.0	30.0	34.0
3	31.59390475953715	31.0	31.0	34.0	30.0	34.0
4	35.305770921961575	37.0	35.0	37.0	33.0	37.0
5	35.11615595048316	37.0	35.0	37.0	32.0	37.0
6	35.22095712801517	37.0	35.0	37.0	32.0	37.0
7	35.64142865873428	37.0	35.0	37.0	35.0	37.0
8	35.67131650937582	37.0	35.0	37.0	35.0	37.0
9	37.151886920465834	39.0	37.0	39.0	34.0	39.0
10	36.65128251342177	38.0	35.0	39.0	32.0	39.0
11	36.32955255233792	38.0	35.0	39.0	32.0	39.0
12	36.2086286744789	38.0	35.0	39.0	32.0	39.0
13	35.90618069647165	38.0	35.0	39.0	31.0	39.0
14	37.07801874645875	39.0	36.0	40.0	31.0	41.0
15	37.223074365372604	39.0	36.0	40.0	32.0	41.0
16	37.236869554769015	39.0	35.0	41.0	32.0	41.0
17	37.25516957061722	39.0	35.0	41.0	32.0	41.0
18	37.20820605567174	39.0	36.0	40.0	32.0	41.0
19	37.19148568119833	39.0	35.0	40.0	32.0	41.0
20	36.80014066576964	39.0	35.0	40.0	31.0	41.0
21	36.87017315976959	39.0	35.0	40.0	31.0	41.0
22	36.96475483447948	39.0	35.0	40.0	31.0	41.0
23	36.80718638421643	39.0	35.0	40.0	31.0	41.0
24	36.849716545008384	39.0	35.0	40.0	31.0	41.0
25	36.852427313190574	39.0	35.0	40.0	31.0	41.0
26	36.789642524567306	39.0	35.0	40.0	31.0	41.0
27	36.55038641653434	39.0	35.0	40.0	30.0	41.0
28	36.525167053549325	38.0	35.0	40.0	30.0	41.0
29	36.56737403525345	38.0	35.0	40.0	30.0	41.0
30	36.42955783507301	38.0	35.0	40.0	30.0	41.0
31	36.3105357418462	38.0	35.0	40.0	30.0	41.0
32	36.01414840756612	38.0	35.0	40.0	29.0	41.0
33	35.92560769580561	38.0	35.0	40.0	29.0	41.0
34	35.86509241161	38.0	35.0	40.0	29.0	41.0
35	35.66298014623854	38.0	34.0	40.0	28.0	41.0
36	35.714591432232346	38.0	34.0	40.0	29.0	41.0
37	35.707092020066106	38.0	34.0	40.0	29.0	41.0
38	35.65622549613687	38.0	34.0	40.0	29.0	41.0
39	35.67345135585021	38.0	34.0	40.0	29.0	41.0
40	35.53394778793237	38.0	34.0	40.0	28.0	41.0
41	35.431131261465346	38.0	34.0	40.0	28.0	41.0
42	35.421792214491475	38.0	34.0	40.0	28.0	41.0
43	35.33912984030603	38.0	34.0	40.0	28.0	41.0
44	35.22815511353219	37.0	34.0	40.0	27.0	41.0
45	35.16157918560941	37.0	34.0	40.0	27.0	41.0
46	35.02544517401433	37.0	34.0	40.0	27.0	41.0
47	34.94768331349718	37.0	33.0	40.0	26.0	41.0
48	34.86452063322385	37.0	33.0	40.0	26.0	41.0
49	34.810204794030305	37.0	33.0	40.0	26.0	41.0
50	34.66650922044439	37.0	33.0	40.0	26.0	41.0
51	33.3640995681623	35.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	18.0
9	67.0
10	83.0
11	45.0
12	27.0
13	25.0
14	36.0
15	67.0
16	101.0
17	228.0
18	481.0
19	754.0
20	1379.0
21	2101.0
22	3061.0
23	4302.0
24	5845.0
25	7835.0
26	10158.0
27	12493.0
28	15720.0
29	19737.0
30	24732.0
31	31501.0
32	40019.0
33	52872.0
34	79929.0
35	89603.0
36	93955.0
37	124432.0
38	173420.0
39	170357.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.44967676912065	20.287256489218557	26.05693545429968	11.206131287361108
2	30.623083066348045	24.45771688476076	26.35763702223617	18.561563026655023
3	31.600699807024796	24.758936367902102	26.583137302428295	17.057226522644804
4	29.631379032099346	26.28896146607293	25.60137724011274	18.47828226171498
5	25.45076749854207	29.702126249081996	24.538718822799453	20.30838742957648
6	25.33392582832768	35.55539672822607	23.528162675094183	15.582514768352066
7	81.83629943371152	6.054221578626261	7.508734639929812	4.600744347732412
8	82.85483147557149	5.290503817552975	6.746674207512049	5.107990499363482
9	76.83531021567025	7.507388060397199	9.600594152323005	6.056707571609546
10	42.570765344014816	25.199578624189336	16.70204027515799	15.52761575663786
11	32.919001169452535	26.2706272678212	21.211527963795657	19.59884359893061
12	28.20472980881678	23.766092920202734	29.054525076936304	18.974652194044182
13	24.80451290592899	26.089149780041414	29.678302149658847	19.428035164370748
14	20.202836310827845	28.57752517326853	28.47777470481423	22.741863811089395
15	19.581234481965677	26.77549100950996	33.58794044803808	20.05533406048628
16	22.65185014848629	25.0217265428435	30.969671921434333	21.356751387235875
17	21.489337679677732	25.042339567996567	27.836077766003836	25.63224498632186
18	22.944057907063222	26.087078119222006	29.005323132475457	21.96354084123931
19	25.311862640601028	26.143945208714648	26.5695679240612	21.97462422662312
20	26.102822741449476	25.980180420940762	27.511241349521292	20.405755488088467
21	24.326477171851515	26.97240237039431	27.54759899690183	21.153521460852343
22	22.985594706492275	26.681230442227076	25.419174671046157	24.914000180234492
23	23.058102835171415	27.056408216621143	26.750423913595174	23.135065034612275
24	22.64594591515099	25.11515844579862	28.323643139850567	23.91525249919982
25	22.033770143017104	27.084479220724067	26.625088434021226	24.2566622022376
26	22.702502255520717	27.75476507884223	26.83784800017402	22.704884665463034
27	22.85311199709139	28.220578014085223	27.256012736570717	21.67029725225267
28	20.191027844157244	27.972807380084504	29.37086768405929	22.465297091698957
29	23.129678716481823	27.47364070564911	26.796932698990787	22.59974787887828
30	23.368126876795223	28.212084204725667	25.806264495151797	22.613524423327316
31	24.27530714961224	28.856267136519342	24.273960570079627	22.594465143788796
32	24.251793799312	29.66390410696399	24.91793633579136	21.16636575793265
33	24.575387219302907	28.603213767429143	24.194926709819363	22.62647230344859
34	22.967053342158607	27.22628440381227	27.482652430213516	22.324009823815604
35	23.570942471014874	26.535281937500066	26.65181285859154	23.24196273289352
36	25.104696558660628	29.467407078243525	25.21035126045024	20.217545102645616
37	23.91939582083863	28.713218956939496	27.661022426764198	19.70636279545768
38	21.64212266510878	30.179022569708795	26.29631586198181	21.88253890320061
39	24.346365115717795	26.48514774567049	26.65792425800878	22.510562880602937
40	25.74100717934057	24.8927656568356	28.15169529184004	21.21453187198379
41	22.54298437242661	25.713557673483468	27.08551505113377	24.657942902956158
42	24.52960351519408	24.516034136826985	27.39595342492146	23.55840892305748
43	23.78038737985662	25.800360261816497	27.617621132597687	22.8016312257292
44	22.42945735952327	26.177402530948022	27.295374292139396	24.097765817389313
45	23.50247408093357	25.707964189271078	25.959670978828665	24.829890750966687
46	23.135065034612275	27.108199737106244	26.548644149785222	23.208091078496267
47	22.447170059529174	26.219353662540957	29.51298361627041	21.820492661659465
48	22.45649253321649	24.490759874830253	29.379775825582733	23.672971766370523
49	23.333944473275057	24.241124746092073	29.76551907015576	22.659411710477116
50	21.750677692045546	25.614532286315956	28.075043841522092	24.559746180116406
51	21.90594867045988	24.49915010114884	26.398241574296488	27.19665965409479
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	319.0
1	559.5
2	800.0
3	1694.5
4	2589.0
5	1935.0
6	1281.0
7	1342.5
8	1404.0
9	1451.5
10	1499.0
11	1624.5
12	1750.0
13	1789.5
14	1829.0
15	1833.5
16	1838.0
17	2074.0
18	2310.0
19	2344.5
20	2379.0
21	2581.0
22	2783.0
23	3218.5
24	3654.0
25	4593.5
26	6431.5
27	7330.0
28	9723.0
29	12116.0
30	14215.5
31	16315.0
32	17129.5
33	17944.0
34	21687.5
35	25431.0
36	26781.5
37	28132.0
38	29389.0
39	30646.0
40	37417.0
41	44188.0
42	51925.0
43	59662.0
44	69371.5
45	79081.0
46	89690.0
47	100299.0
48	99208.5
49	98118.0
50	85408.5
51	72699.0
52	65676.5
53	58654.0
54	53278.0
55	47902.0
56	45798.0
57	43694.0
58	41435.0
59	39176.0
60	39266.5
61	39357.0
62	35929.0
63	32501.0
64	29700.0
65	26899.0
66	22072.5
67	17246.0
68	15026.5
69	12807.0
70	10935.5
71	9064.0
72	7482.5
73	5901.0
74	5053.0
75	3551.0
76	2897.0
77	2279.5
78	1662.0
79	1196.5
80	731.0
81	562.0
82	393.0
83	286.0
84	179.0
85	144.0
86	109.0
87	79.0
88	49.0
89	41.0
90	33.0
91	20.5
92	8.0
93	6.0
94	4.0
95	3.5
96	3.0
97	4.5
98	6.0
99	3.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	965409.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.105417017262532
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.92094101404939	18.514207404284374
2	11.766942649857013	6.143618897854592
3	4.943873763483541	3.871856588293231
4	2.509775664863558	2.6207496140416207
5	1.5676131409562397	2.0461597383201697
6	0.9721713694335459	1.5227363406783547
7	0.6498702030313359	1.1875592860058264
8	0.47786463758367037	0.9979884513539795
9	0.36179432916828536	0.8500312653676956
>10	4.169182637208398	28.17521584427074
>50	1.5027611374015326	26.26434940212145
>100	0.1499536320574785	5.6021954811095425
>500	0.004434112775893181	0.7419854675752462
>1k	0.0028217081301138425	1.461346218723223
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	3738	0.3871934071466083	TruSeq Adapter, Index 15 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	2723	0.28205662056185515	TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT	2181	0.22591461235600663	TruSeq Adapter, Index 21 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	1661	0.17205143105150253	TruSeq Adapter, Index 15 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	1368	0.14170160004723387	TruSeq Adapter, Index 21 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1199	0.12419606612327004	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1017	0.1053439526666936	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2689015743586397	0.0
2	0.0	0.0	0.0	1.4159801700626367	0.0
3	0.0	0.0	0.0	1.880239359691074	0.0
4	0.0	0.0	0.0	2.3870711791582635	0.0
5	0.0	0.0	0.0	3.6698435585332225	0.0
6	0.0	0.0	0.0	4.337643423668103	0.0
7	0.0	0.0	0.0	5.085616562513919	0.0
8	0.0	0.0	0.0	6.270813717294949	0.0
9	0.0	0.0	0.0	6.69001428410135	0.0
10	0.0	0.0	0.0	7.832638809043628	0.0
11	0.0	0.0	0.0	9.471426100233165	0.0
12	0.0	0.0	0.0	10.66915680297159	0.0
13	0.0	0.0	0.0	11.150507194360111	0.0
14	0.0	0.0	0.0	11.330638102607288	0.0
15	0.0	0.0	0.0	11.70332988401807	0.0
16	0.0	0.0	0.0	12.573738177290661	0.0
17	0.0	0.0	0.0	13.607393343132289	0.0
18	0.0	0.0	0.0	14.786893430659958	0.0
19	0.0	0.0	0.0	15.399276368875782	0.0
20	0.0	0.0	0.0	16.00378699597787	0.0
21	4.143321638808008E-4	0.0	0.0	16.772476743017727	0.0
22	4.143321638808008E-4	0.0	0.0	17.548210136843554	0.0
23	9.322473687318018E-4	0.0	0.0	18.34207056283917	0.0
24	0.001553745614553003	0.0	0.0	18.950413762457156	0.0
25	0.0027967421061954054	0.0	0.0	19.53296478487356	0.0
26	0.0027967421061954054	0.0	0.0	20.047565332413516	0.0
27	0.0027967421061954054	0.0	0.0	20.561544381707648	0.0
28	0.003107491229106006	0.0	0.0	21.086606816385594	0.0
29	0.0035218233929868066	0.0	0.0	21.625860127676457	0.0
30	0.003625406433957007	0.0	0.0	22.24839420390736	0.0
31	0.003728989474927207	0.0	0.0	22.83757454094586	0.0
32	0.003936155556867608	0.0	0.0	23.441981585006975	0.0
33	0.004039738597837807	0.0	0.0	23.96082903722671	0.0
34	0.004246904679778208	0.0	0.0	24.4989429350669	0.0
35	0.004661236843659009	0.0	0.0	25.090920014211594	0.0
36	0.004971985966569609	0.0	0.0	25.719047574654887	0.0
37	0.004971985966569609	0.0	0.0	26.281917819286956	0.0
38	0.005075569007539809	0.0	0.0	26.830182855142226	0.0
39	0.005075569007539809	0.0	0.0	27.39688567229019	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTACG	20	7.032724E-4	45.000004	15
GTCGACA	20	7.032724E-4	45.000004	23
GTTAGAT	20	7.032724E-4	45.000004	18
ATCACGC	20	7.032724E-4	45.000004	25
TCAATCG	20	7.032724E-4	45.000004	43
GAGCGTA	20	7.032724E-4	45.000004	9
ACGTCTA	20	7.032724E-4	45.000004	11
CGTCCAT	20	7.032724E-4	45.000004	27
TCGAATC	20	7.032724E-4	45.000004	29
TGCGTGA	20	7.032724E-4	45.000004	40
GATCGAG	25	3.890341E-5	45.0	1
TCGTCCA	25	3.890341E-5	45.0	26
TCGTAAG	25	3.890341E-5	45.0	1
TTACGAG	45	3.8380676E-10	45.0	1
ACGTAAC	25	3.890341E-5	45.0	11
TATCGAT	25	3.890341E-5	45.0	34
TAATGCG	45	3.8380676E-10	45.0	1
ATTAGTC	60	3.6379788E-12	41.249996	31
AATACGG	60	3.6379788E-12	41.249996	2
ATTAGCG	55	6.002665E-11	40.909092	1
>>END_MODULE