FastQCFastQC Report
Sat 18 Jun 2016
SRR3552373_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552373_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1098432
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC18820.17133513954436871Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC14650.13337193381110526Illumina Single End Adapter 1 (95% over 21bp)
GAGGAGGGGAAGAAGATTGATCAAACTGAATGAAACACACACACACACACA13840.12599778593485988No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAACG302.1654141E-645.00000419
GTATACG453.8562575E-1045.0000041
CGGAATA207.033151E-445.019
AGCCGAT253.8906925E-545.042
CGAATAT1000.045.014
CGAATAC207.033151E-445.045
TCGTAAG253.8906925E-545.01
ATTAGCG950.040.2631571
ATTACGG451.9281288E-840.0000042
TATAGCG403.4580444E-739.3751
TCGTTAG700.038.571431
ACTACGT356.248036E-638.5714314
CGTAAAT356.248036E-638.5714315
GCGTACG356.248036E-638.571431
GCTACGA1350.038.33333210
AACCGTT301.13988885E-437.50000427
TCGCACG301.13988885E-437.5000041
CGGTACA301.13988885E-437.50000417
CTACGAA1450.037.24138311
CGATGTA850.037.05882310
Produced by FastQC (version 0.11.5)