##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552373_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1098432
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.47343577026161	31.0	31.0	34.0	30.0	34.0
2	31.70035013546583	31.0	31.0	34.0	30.0	34.0
3	31.768842313406747	31.0	31.0	34.0	30.0	34.0
4	35.467303392472175	37.0	35.0	37.0	33.0	37.0
5	35.26780538076094	37.0	35.0	37.0	33.0	37.0
6	35.35380342160462	37.0	35.0	37.0	33.0	37.0
7	35.667252046553635	37.0	35.0	37.0	35.0	37.0
8	35.69760895531084	37.0	35.0	37.0	35.0	37.0
9	37.205553916856026	39.0	37.0	39.0	34.0	39.0
10	36.73207535832896	39.0	37.0	39.0	32.0	39.0
11	36.42276627046554	38.0	35.0	39.0	32.0	39.0
12	36.294848474916975	38.0	35.0	39.0	32.0	39.0
13	36.01396536153353	38.0	35.0	39.0	31.0	39.0
14	37.22083934189827	39.0	36.0	41.0	32.0	41.0
15	37.342519154576706	39.0	36.0	41.0	32.0	41.0
16	37.336083617374584	39.0	36.0	41.0	32.0	41.0
17	37.31308446949834	39.0	36.0	41.0	32.0	41.0
18	37.28292238390724	39.0	36.0	41.0	32.0	41.0
19	37.24613084833654	39.0	35.0	41.0	32.0	41.0
20	36.89616198362757	39.0	35.0	41.0	31.0	41.0
21	36.957780727436926	39.0	35.0	40.0	31.0	41.0
22	37.038480306473225	39.0	35.0	40.0	31.0	41.0
23	36.89252407067529	39.0	35.0	40.0	31.0	41.0
24	36.93712218871992	39.0	35.0	40.0	31.0	41.0
25	36.946773218551535	39.0	35.0	40.0	31.0	41.0
26	36.88058068228165	39.0	35.0	40.0	31.0	41.0
27	36.665545067878575	39.0	35.0	40.0	31.0	41.0
28	36.62679073442871	39.0	35.0	40.0	31.0	41.0
29	36.6651262891103	39.0	35.0	40.0	31.0	41.0
30	36.53954546116646	38.0	35.0	40.0	30.0	41.0
31	36.41891896812911	38.0	35.0	40.0	30.0	41.0
32	36.18296262308454	38.0	35.0	40.0	30.0	41.0
33	36.11382953155043	38.0	35.0	40.0	30.0	41.0
34	36.004289751208994	38.0	35.0	40.0	29.0	41.0
35	35.83738274194488	38.0	34.0	40.0	29.0	41.0
36	35.868486169375984	38.0	34.0	40.0	29.0	41.0
37	35.89693308279438	38.0	34.0	40.0	29.0	41.0
38	35.86659711297559	38.0	34.0	40.0	29.0	41.0
39	35.802129763153296	38.0	34.0	40.0	29.0	41.0
40	35.65889376857193	38.0	34.0	40.0	29.0	41.0
41	35.5452927445668	38.0	34.0	40.0	28.0	41.0
42	35.54378240983511	38.0	34.0	40.0	28.0	41.0
43	35.45885225485055	38.0	34.0	40.0	28.0	41.0
44	35.36775148575424	37.0	34.0	40.0	28.0	41.0
45	35.28387009846763	37.0	34.0	40.0	28.0	41.0
46	35.14948672289227	37.0	34.0	40.0	27.0	41.0
47	35.028530669172056	37.0	34.0	40.0	27.0	41.0
48	34.95681025316087	37.0	34.0	40.0	27.0	41.0
49	34.89183399609625	37.0	34.0	40.0	27.0	41.0
50	34.779939040377556	36.0	33.0	40.0	27.0	41.0
51	33.52280432471013	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	31.0
9	87.0
10	98.0
11	54.0
12	53.0
13	25.0
14	33.0
15	65.0
16	116.0
17	228.0
18	485.0
19	825.0
20	1404.0
21	2181.0
22	3105.0
23	4482.0
24	6320.0
25	8409.0
26	10883.0
27	13585.0
28	17023.0
29	21125.0
30	26893.0
31	34022.0
32	44167.0
33	58545.0
34	92246.0
35	100201.0
36	104635.0
37	139787.0
38	199480.0
39	207803.0
40	31.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.618081046437105	21.458770319874148	26.389617199790244	11.533531433898503
2	30.87610339101556	24.830121482258345	26.297758987356524	17.996016139369576
3	32.768619268193206	24.979789372487325	25.167238390724233	17.084352968595233
4	29.367953592029366	26.946866078191455	25.295603187088506	18.389577142690673
5	26.290111722892266	30.134136660257532	23.795920002330597	19.779831614519605
6	26.752771222979664	35.73229840354251	22.409216046145776	15.10571432733205
7	83.73973081629086	5.996638845190235	6.167700868146595	4.095929470372313
8	86.13705718697197	4.728831643652042	5.283167278447824	3.8509438909281593
9	80.07213919477947	7.121879187787683	7.44998324884927	5.355998368583581
10	44.9778411408262	25.247261551010897	14.62302627745732	15.151871030705589
11	34.085769533298375	26.063789110295403	20.628040698013166	19.222400658393056
12	30.904234399580492	22.440624453766826	26.715263211559755	19.93987793509293
13	25.936061585969817	23.862378372079473	28.004464545825318	22.197095496125385
14	21.79952878284682	26.388706811163548	28.508000495251412	23.303763910738216
15	22.05443759832197	25.06882538017829	31.307900716657926	21.56883630484181
16	25.749249839771604	24.292810114781798	28.954819233234286	21.003120812212316
17	24.879282468099984	23.865291615684903	26.96953475499621	24.2858911612189
18	25.59648662821185	24.650228689623027	27.665253743518033	22.08803093864709
19	26.903713657286023	26.6556327565111	24.776863747596575	21.663789838606302
20	28.088402377206783	26.063333915982057	25.07792926644526	20.770334440365904
21	26.926291295228104	26.86429382975004	25.399660607119966	20.80975426790188
22	25.058446949833947	24.968682631241624	25.07574433374119	24.897126085183245
23	25.576184961836507	26.285377702033447	25.73067791178698	22.407759424343062
24	25.42633499388219	24.29007894890171	26.989927460234224	23.293658596981878
25	24.200041513721377	25.84939258870827	25.00291324360543	24.947652653964923
26	23.12869617782439	26.96061294645458	25.531393841403016	24.37929703431801
27	23.2512344869778	26.927656878168154	25.709465856784945	24.111642778069104
28	22.053527209695275	28.34394846472062	25.852852065489717	23.74967226009439
29	24.08278345860281	25.970656353784307	26.165843675348132	23.780716512264753
30	24.93017319233234	25.190726417293014	26.4753758084251	23.403724581949543
31	25.953450008739733	26.691866223853637	24.233088620870475	23.12159514653615
32	26.704702703490067	26.386886033910155	24.499650410767348	22.40876085183243
33	25.64856085765892	25.59102429645167	24.522045970984095	24.23836887490532
34	23.57132712812445	26.644343937540054	26.65636106741246	23.127967866923033
35	23.52771951290567	25.747975295694225	26.640884460758606	24.083420730641496
36	23.826326982462273	28.66449630018062	25.000637272038688	22.50853944531842
37	24.953479141175787	27.92362203577463	24.816010458544543	22.30688836450504
38	24.347524471246285	27.247749519314805	24.566563974829574	23.838162034609333
39	24.956574462506556	25.38236322321272	26.33763400920585	23.32342830507487
40	24.78132465186739	25.630535162850315	27.23955602167453	22.34858416360776
41	21.942641874963584	26.209633368292256	26.88668938996679	24.96103536677737
42	23.513426411466526	24.951112130746374	26.691684146128296	24.8437773116588
43	23.694775825904564	23.753131736875837	27.285621686185397	25.266470751034202
44	23.451337907125794	24.891572714560393	26.10093296626464	25.556156412049173
45	23.68039168560275	24.78787944997961	25.723667919361414	25.808060945056226
46	23.583799452310203	26.062969760531374	25.435711996737165	24.917518790421255
47	22.584465856784945	25.81916768630193	27.378026131795142	24.21834032511799
48	22.97283764493387	24.843413156208122	28.309899930082157	23.873849268775853
49	23.62868161160636	24.58021980423003	27.74400235972732	24.047096224436288
50	21.912963205733263	24.728066917205616	27.37920963700985	25.979760240051274
51	21.748000786575776	24.44775825904562	26.716901911087803	27.087339043290797
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	254.0
1	572.5
2	891.0
3	1597.5
4	2304.0
5	1811.5
6	1319.0
7	1307.5
8	1296.0
9	1366.5
10	1437.0
11	1529.5
12	1622.0
13	1858.0
14	2094.0
15	2131.5
16	2169.0
17	2321.0
18	2473.0
19	2662.5
20	2852.0
21	3025.0
22	3198.0
23	3715.5
24	4233.0
25	5454.0
26	7683.5
27	8692.0
28	11449.5
29	14207.0
30	15953.5
31	17700.0
32	19698.5
33	21697.0
34	22704.0
35	23711.0
36	26099.5
37	28488.0
38	31156.5
39	33825.0
40	38763.0
41	43701.0
42	48042.0
43	52383.0
44	57980.0
45	63577.0
46	68308.0
47	73039.0
48	78677.0
49	84315.0
50	86278.5
51	88242.0
52	81786.0
53	75330.0
54	72778.5
55	70227.0
56	65776.0
57	61325.0
58	61177.0
59	61029.0
60	62231.5
61	63434.0
62	58250.5
63	53067.0
64	45568.0
65	38069.0
66	32683.5
67	27298.0
68	23868.5
69	20439.0
70	17237.5
71	14036.0
72	12136.5
73	10237.0
74	8603.0
75	5780.5
76	4592.0
77	3775.5
78	2959.0
79	2215.5
80	1472.0
81	1142.0
82	812.0
83	630.0
84	448.0
85	290.0
86	132.0
87	117.5
88	103.0
89	68.0
90	33.0
91	23.5
92	14.0
93	12.0
94	10.0
95	6.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1098432.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.99426655718042
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.53193364505101	17.643448163442574
2	10.285082560522095	4.935660250395498
3	3.572416740737162	2.5715255859174344
4	1.7836466194608425	1.7118916972462883
5	1.023852118798592	1.2283290326793685
6	0.7174138897699397	1.032829206177815
7	0.5166241476757812	0.8677212256446185
8	0.4157380639597519	0.7980263939693126
9	0.32311091078990734	0.6977528388923742
>10	6.15545277569854	39.04546649118797
>50	1.5638499511179695	24.290926100008996
>100	0.10471865550625617	3.8887836326430567
>500	0.004619940684099529	0.755734111662418
>1k	0.0015399802280331764	0.5319052701322825
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC	1882	0.17133513954436871	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC	1465	0.13337193381110526	Illumina Single End Adapter 1 (95% over 21bp)
GAGGAGGGGAAGAAGATTGATCAAACTGAATGAAACACACACACACACACA	1384	0.12599778593485988	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.103886266969644E-5	0.0	0.0	0.08621380294820252	0.0
2	9.103886266969644E-5	0.0	0.0	0.5601621220066422	0.0
3	9.103886266969644E-5	0.0	0.0	0.7629056691720562	0.0
4	9.103886266969644E-5	0.0	0.0	1.0015185282293306	0.0
5	9.103886266969644E-5	0.0	0.0	1.6934138845190234	0.0
6	9.103886266969644E-5	0.0	0.0	2.0887046262308453	0.0
7	9.103886266969644E-5	0.0	0.0	2.5241435063800033	0.0
8	9.103886266969644E-5	0.0	0.0	3.2469920759773934	0.0
9	9.103886266969644E-5	0.0	0.0	3.504905173920643	0.0
10	9.103886266969644E-5	0.0	0.0	4.230940103711473	0.0
11	9.103886266969644E-5	0.0	0.0	5.249391860397367	0.0
12	9.103886266969644E-5	0.0	0.0	5.968963030938647	0.0
13	9.103886266969644E-5	0.0	0.0	6.26192609100973	0.0
14	9.103886266969644E-5	0.0	0.0	6.37308454232943	0.0
15	9.103886266969644E-5	0.0	0.0	6.586479636427198	0.0
16	9.103886266969644E-5	0.0	0.0	7.090288702441298	0.0
17	9.103886266969644E-5	0.0	0.0	7.6677482083551824	0.0
18	9.103886266969644E-5	0.0	0.0	8.348081629085824	0.0
19	9.103886266969644E-5	0.0	0.0	8.709505913884518	0.0
20	9.103886266969644E-5	0.0	0.0	9.08085343471421	0.0
21	1.8207772533939288E-4	0.0	0.0	9.536320864650703	0.0
22	5.462331760181787E-4	0.0	0.0	10.007173862378371	0.0
23	6.372720386878751E-4	0.0	0.0	10.488951523626406	0.0
24	0.0010924663520363574	0.0	0.0	10.870859552525783	0.0
25	0.0011835052147060538	0.0	0.0	11.218628007924023	0.0
26	0.0012745440773757502	0.0	0.0	11.547642457612305	0.0
27	0.0012745440773757502	0.0	0.0	11.857083551826603	0.0
28	0.0012745440773757502	0.0	0.0	12.202302919070092	0.0
29	0.0013655829400454466	0.0	0.0	12.563272009555439	0.0
30	0.0013655829400454466	0.0	0.0	12.975131824273145	0.0
31	0.0013655829400454466	0.0	0.0	13.349301549845599	0.0
32	0.001456621802715143	0.0	0.0	13.72319815883004	0.0
33	0.001456621802715143	0.0	0.0	14.089993736526248	0.0
34	0.001456621802715143	0.0	0.0	14.46434553982404	0.0
35	0.001456621802715143	0.0	0.0	14.862731602866631	0.0
36	0.001456621802715143	0.0	0.0	15.267945580609451	0.0
37	0.001456621802715143	0.0	0.0	15.678530851249782	0.0
38	0.001456621802715143	0.0	0.0	16.115881547515002	0.0
39	0.001456621802715143	0.0	0.0	16.568344694983395	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAAACG	30	2.1654141E-6	45.000004	19
GTATACG	45	3.8562575E-10	45.000004	1
CGGAATA	20	7.033151E-4	45.0	19
AGCCGAT	25	3.8906925E-5	45.0	42
CGAATAT	100	0.0	45.0	14
CGAATAC	20	7.033151E-4	45.0	45
TCGTAAG	25	3.8906925E-5	45.0	1
ATTAGCG	95	0.0	40.263157	1
ATTACGG	45	1.9281288E-8	40.000004	2
TATAGCG	40	3.4580444E-7	39.375	1
TCGTTAG	70	0.0	38.57143	1
ACTACGT	35	6.248036E-6	38.57143	14
CGTAAAT	35	6.248036E-6	38.57143	15
GCGTACG	35	6.248036E-6	38.57143	1
GCTACGA	135	0.0	38.333332	10
AACCGTT	30	1.13988885E-4	37.500004	27
TCGCACG	30	1.13988885E-4	37.500004	1
CGGTACA	30	1.13988885E-4	37.500004	17
CTACGAA	145	0.0	37.241383	11
CGATGTA	85	0.0	37.058823	10
>>END_MODULE