FastQCFastQC Report
Sat 18 Jun 2016
SRR3552371_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552371_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2506590
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC54030.21555180544085792No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38060.15183975041789843No Hit
CCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC36360.14505762809234857No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT34930.1393526663714449No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA26760.10675858437159648No Hit
TCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC25870.10320794385998508No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGCG650.041.5384641
GTACGCG850.039.7058831
TACTACG900.037.5000041
TTTAGCG1500.037.51
TAGGGCG9650.036.6062165
TAGTTCG504.882895E-836.01
GTAGCGT250.00210758236.015
TCGTCAG250.00210758236.038
ATACCGG1500.036.02
CGTTAGG3900.035.769232
TAACGGG8650.035.6358383
CTACGAA2150.035.58139811
CGAATAT2150.035.58139814
TGTAGCG3100.035.5645141
ATTAGCG653.6743586E-1034.6153871
TTGCGAG2600.034.6153871
TAAGGGA21600.034.5833364
TTACGAG850.034.4117621
TCGATAG1050.034.2857131
AGGGCGA25100.034.2430276
Produced by FastQC (version 0.11.5)