FastQCFastQC Report
Sat 18 Jun 2016
SRR3552370_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552370_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1400607
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC39270.2803784359209971No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC33600.23989598795379433No Hit
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC27670.1975572019845681No Hit
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT23990.17128287949439064Illumina Single End Adapter 2 (95% over 21bp)
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG16690.11916262020681033No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG15750.11245124435334108No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA15630.11159447296779182No Hit
TCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC15110.10788179696374502No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAAAC302.1658434E-645.00000410
CTATACG207.033815E-445.026
TATATCG207.033815E-445.01
TACGTAG207.033815E-445.01
CGCTTAA207.033815E-445.044
TTAGCCG556.184564E-1140.9090921
TAGAACG556.184564E-1140.9090921
TACGGGT1600.039.3754
CCCTCGT5350.038.6915914
CGTGTAC5350.038.6915918
CGAAACA356.2492672E-638.5714311
CTATCGG356.2492672E-638.571432
CGATTAC356.2492672E-638.5714310
ACCGTAG356.2492672E-638.571431
TGTAACG700.038.571431
TCTATCG356.2492672E-638.571431
AGGGTAC5100.038.3823556
ATTACGG601.5643309E-1037.5000042
CCAATCG301.14004906E-437.5000041
ACTTCGA301.14004906E-437.50000437