##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552370_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1400607
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.401867904415727	31.0	31.0	34.0	30.0	34.0
2	31.615373191766142	31.0	31.0	34.0	30.0	34.0
3	31.701498707346172	31.0	31.0	34.0	30.0	34.0
4	35.40044566391572	37.0	35.0	37.0	33.0	37.0
5	35.206666109765266	37.0	35.0	37.0	33.0	37.0
6	35.291628558189416	37.0	35.0	37.0	32.0	37.0
7	35.614938380287974	37.0	35.0	37.0	35.0	37.0
8	35.6423936193379	37.0	35.0	37.0	35.0	37.0
9	37.13131235243005	39.0	37.0	39.0	34.0	39.0
10	36.661826622314464	38.0	35.0	39.0	32.0	39.0
11	36.387149285988144	38.0	35.0	39.0	32.0	39.0
12	36.31071956658791	38.0	35.0	39.0	32.0	39.0
13	36.04042961373176	38.0	35.0	39.0	31.0	39.0
14	37.248608638968676	39.0	36.0	41.0	32.0	41.0
15	37.35978686383832	39.0	36.0	41.0	32.0	41.0
16	37.33483839506728	39.0	36.0	41.0	32.0	41.0
17	37.33837257703267	39.0	36.0	41.0	32.0	41.0
18	37.30658350272417	39.0	36.0	41.0	32.0	41.0
19	37.26814516848766	39.0	36.0	41.0	32.0	41.0
20	36.90369532638349	39.0	35.0	40.0	31.0	41.0
21	36.958958508703724	39.0	35.0	40.0	31.0	41.0
22	37.03334768425404	39.0	35.0	40.0	31.0	41.0
23	36.88444795720712	39.0	35.0	40.0	31.0	41.0
24	36.90414227545629	39.0	35.0	40.0	31.0	41.0
25	36.88549036239288	39.0	35.0	40.0	31.0	41.0
26	36.82375355827866	39.0	35.0	40.0	31.0	41.0
27	36.61013689064813	39.0	35.0	40.0	30.0	41.0
28	36.5613673214542	39.0	35.0	40.0	30.0	41.0
29	36.58831420948203	38.0	35.0	40.0	30.0	41.0
30	36.452082561346614	38.0	35.0	40.0	30.0	41.0
31	36.366206937420706	38.0	35.0	40.0	30.0	41.0
32	36.12575119216169	38.0	35.0	40.0	30.0	41.0
33	36.019650051727574	38.0	35.0	40.0	29.0	41.0
34	35.919019396590194	38.0	35.0	40.0	29.0	41.0
35	35.77054519933143	38.0	34.0	40.0	28.0	41.0
36	35.78436420780419	38.0	34.0	40.0	29.0	41.0
37	35.7381413915538	38.0	34.0	40.0	29.0	41.0
38	35.66941833076659	38.0	34.0	40.0	29.0	41.0
39	35.63288417093446	38.0	34.0	40.0	28.0	41.0
40	35.49305979478898	38.0	34.0	40.0	28.0	41.0
41	35.41187999203203	38.0	34.0	40.0	28.0	41.0
42	35.39857433241445	38.0	34.0	40.0	28.0	41.0
43	35.28082181511302	37.0	34.0	40.0	27.0	41.0
44	35.177116778653826	37.0	34.0	40.0	27.0	41.0
45	35.105579937841235	37.0	34.0	40.0	27.0	41.0
46	34.97998082260049	37.0	34.0	40.0	27.0	41.0
47	34.88020693884866	37.0	33.0	40.0	26.0	41.0
48	34.799103531540254	37.0	33.0	40.0	26.0	41.0
49	34.71076825976166	36.0	33.0	40.0	26.0	41.0
50	34.583852572491786	36.0	33.0	40.0	26.0	41.0
51	33.30940299455879	35.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	25.0
9	82.0
10	113.0
11	78.0
12	53.0
13	40.0
14	43.0
15	96.0
16	168.0
17	301.0
18	635.0
19	1094.0
20	1854.0
21	2946.0
22	4454.0
23	6222.0
24	8538.0
25	11467.0
26	14268.0
27	17843.0
28	22246.0
29	27849.0
30	35229.0
31	44774.0
32	56904.0
33	75924.0
34	112975.0
35	127118.0
36	140845.0
37	186888.0
38	254437.0
39	245072.0
40	25.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.978233008973966	21.12369851071714	25.823446548532168	12.074621931776724
2	28.974508909351442	25.767970601317856	27.02721034522889	18.230310144101807
3	31.999911466956828	24.408916990990335	26.738406990683327	16.852764551369514
4	29.851343024845654	26.33508186093601	25.12403550746212	18.689539606756213
5	25.22449195241777	30.285012141164508	24.071634655545772	20.418861250871945
6	25.9958003922585	36.38943686558756	22.459190907942055	15.155571834211882
7	83.065485178926	6.554729485144656	6.25478810258695	4.124997233342401
8	84.54955601392825	5.034959842411183	6.187103163128558	4.2283809805320125
9	78.38358654497657	7.340388845693331	8.332387314928456	5.943637294401642
10	42.859560176409225	25.4970166506379	15.8464865590419	15.796936613910969
11	32.373963574364545	27.135306335039022	20.150763204810485	20.339966885785948
12	27.25511153378499	23.821243218119	28.11131173841056	20.812333509685445
13	24.460180478892365	25.431188049181536	29.00392472692197	21.10470674500413
14	21.85973652851942	27.137948046811132	28.236757348778063	22.76555807589138
15	22.091207597848648	24.8862814479722	30.851409424628034	22.171101529551116
16	23.624542787519985	25.268972666850875	28.51692159185267	22.589562953776472
17	23.735923067641387	24.66180734495829	26.96195292469622	24.640316662704098
18	24.952895423198658	24.25498373205332	27.47158910386711	23.320531740880917
19	25.587905815121587	25.41498079047156	25.68807666961539	23.309036724791465
20	26.439750765203947	25.979307543086676	25.678580786758882	21.902360904950495
21	25.42761816840841	26.21313473372616	26.641663221731722	21.717583876133705
22	24.56606314262316	24.375502906953912	25.17165771697557	25.886776233447357
23	24.131037471610522	26.30987850267777	25.468457604452926	24.090626421258783
24	23.736565646180548	25.060348834469625	27.280529084889622	23.9225564344602
25	23.935907788551678	26.145592589498694	25.73584167435976	24.182657947589867
26	23.250133692034954	26.419902228105386	26.354930398034565	23.975033681825096
27	23.77062230875613	25.679723148606282	25.881564207518597	24.66809033511899
28	20.632125928258247	27.264036235717796	27.16893461192183	24.93490322410212
29	23.037582990803273	25.37264200450233	26.533638629537048	25.056136375157344
30	23.419203245450007	25.643381762335903	26.05063376093365	24.886781231280438
31	24.244131294503028	26.478733863246433	23.84316228606597	25.43397255618457
32	24.4032765793688	26.492799193492534	24.022441698492152	25.08148252864651
33	23.732138994021877	26.372280018591937	23.145036402074243	26.750544585311943
34	22.037516591020893	25.836512312161798	26.979659533330906	25.1463115634864
35	23.263984829434666	24.432264011246552	26.318517614148725	25.98523354517006
36	24.386640934966053	27.118028112097115	24.896848295060643	23.598482657876193
37	23.6636686807934	25.84850711155949	25.791317621574073	24.69650658607304
38	23.61369034996969	27.346500481576918	24.276974197615747	24.762834970837645
39	24.439475170408258	24.933618067023797	26.041566263769923	24.585340498798022
40	24.097623387574103	24.55920897153877	28.347923436053087	22.995244204834048
41	22.797330014772164	24.916197048850965	26.340293886864767	25.946179049512104
42	24.300535410718354	24.06006824184086	26.25661588154279	25.382780465897998
43	24.7095009520872	22.476469130883967	26.60239453322738	26.21163538380145
44	23.000956014071043	24.22642468586834	25.40005868883991	27.37256061122071
45	23.36829674562529	23.65959901671204	26.41033494763342	26.561769290029254
46	23.56449739291607	25.00080322317395	26.095114475366753	25.339584908543223
47	22.476683323730352	24.803888599728545	28.236043372623442	24.483384703917658
48	22.888004986409463	24.77468697500441	26.57433527035064	25.762972768235485
49	23.189160128430032	24.709072566394426	27.497006654971738	24.604760650203804
50	22.409997950888435	23.886572036267133	27.747755080475823	25.95567493236861
51	22.78512102252809	23.835736934057877	25.12110820522816	28.25803383818587
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	570.0
1	849.0
2	1128.0
3	2060.5
4	2993.0
5	2355.5
6	1718.0
7	1675.0
8	1632.0
9	1807.5
10	1983.0
11	2169.5
12	2356.0
13	2588.0
14	2820.0
15	2717.5
16	2615.0
17	2701.5
18	2788.0
19	2766.0
20	2744.0
21	3195.0
22	3646.0
23	4005.0
24	4364.0
25	6556.0
26	10477.0
27	12206.0
28	14733.0
29	17260.0
30	19950.0
31	22640.0
32	25190.0
33	27740.0
34	30415.5
35	33091.0
36	34323.0
37	35555.0
38	38318.0
39	41081.0
40	45605.5
41	50130.0
42	55153.0
43	60176.0
44	66471.5
45	72767.0
46	80595.5
47	88424.0
48	97203.0
49	105982.0
50	116123.5
51	126265.0
52	125020.0
53	123775.0
54	112099.5
55	100424.0
56	90804.5
57	81185.0
58	76308.0
59	71431.0
60	68985.5
61	66540.0
62	61961.5
63	57383.0
64	50513.0
65	43643.0
66	38693.0
67	33743.0
68	30301.0
69	26859.0
70	23253.0
71	19647.0
72	17902.5
73	16158.0
74	13448.5
75	8686.5
76	6634.0
77	5470.0
78	4306.0
79	3299.5
80	2293.0
81	1751.5
82	1210.0
83	1000.5
84	791.0
85	512.0
86	233.0
87	164.5
88	96.0
89	69.0
90	42.0
91	34.0
92	26.0
93	21.5
94	17.0
95	12.5
96	8.0
97	5.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1400607.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.088097500404576
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.24424715512554	18.375588139715198
2	10.770220807483781	5.404086994380785
3	4.056913362039334	3.053407139326109
4	2.041275604074084	2.0484688552083146
5	1.1119245765528676	1.3948036094827208
6	0.7225948800554569	1.0877118482474673
7	0.5476482759409763	0.961761733991498
8	0.40794997296868296	0.8187750957700585
9	0.3304037032224436	0.7460280288845464
>10	5.126259384007958	34.21698492891174
>50	1.515340411667969	25.08396935173003
>100	0.11831307420825815	4.577388066025701
>500	0.0034543963263163023	0.5406403662930963
>1k	0.0034543963263163023	1.6903858420326225
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC	3927	0.2803784359209971	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	3360	0.23989598795379433	No Hit
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC	2767	0.1975572019845681	No Hit
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT	2399	0.17128287949439064	Illumina Single End Adapter 2 (95% over 21bp)
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1669	0.11916262020681033	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1575	0.11245124435334108	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1563	0.11159447296779182	No Hit
TCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC	1511	0.10788179696374502	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.139761546243878E-5	0.0	0.0	0.19962773283297885	0.0
2	7.139761546243878E-5	0.0	0.0	0.9592983613533275	0.0
3	7.139761546243878E-5	0.0	0.0	1.2946529611804025	0.0
4	7.139761546243878E-5	0.0	0.0	1.6594233785780022	0.0
5	7.139761546243878E-5	0.0	0.0	2.6207922707797406	0.0
6	7.139761546243878E-5	0.0	0.0	3.185047625779394	0.0
7	7.139761546243878E-5	0.0	0.0	3.7887858621297767	0.0
8	7.139761546243878E-5	0.0	0.0	4.794635468764614	0.0
9	7.139761546243878E-5	0.0	0.0	5.139557349063656	0.0
10	7.139761546243878E-5	0.0	0.0	6.014749319402231	0.0
11	7.139761546243878E-5	0.0	0.0	7.308973894889858	0.0
12	7.139761546243878E-5	0.0	0.0	8.273555679787407	0.0
13	7.139761546243878E-5	0.0	0.0	8.655604320126917	0.0
14	7.139761546243878E-5	0.0	0.0	8.80975177191032	0.0
15	7.139761546243878E-5	0.0	0.0	9.111192504392738	0.0
16	7.139761546243878E-5	0.0	0.0	9.743918172620871	0.0
17	7.139761546243878E-5	0.0	0.0	10.509871791301913	0.0
18	7.139761546243878E-5	0.0	0.0	11.396201789652629	0.0
19	7.139761546243878E-5	0.0	0.0	11.883847503261086	0.0
20	7.139761546243878E-5	0.0	0.0	12.35264424638746	0.0
21	4.2838569277463273E-4	0.0	0.0	12.921968832084946	0.0
22	4.2838569277463273E-4	0.0	0.0	13.518710102120009	0.0
23	5.711809236995102E-4	0.0	0.0	14.105598501221257	0.0
24	7.853737700868267E-4	0.0	0.0	14.622374441938389	0.0
25	0.0012137594628614592	0.0	0.0	15.056828932027328	0.0
26	0.0012137594628614592	0.0	0.0	15.457440952387072	0.0
27	0.0012137594628614592	0.0	0.0	15.866406493755921	0.0
28	0.0012137594628614592	0.0	0.0	16.282725989517402	0.0
29	0.0012137594628614592	0.0	0.0	16.71632370822079	0.0
30	0.0012137594628614592	0.0	0.0	17.194187948510894	0.0
31	0.001356554693786337	0.0	0.0	17.62878523383076	0.0
32	0.001356554693786337	0.0	0.0	18.046675477132414	0.0
33	0.001356554693786337	0.0	0.0	18.49876517824058	0.0
34	0.0014993499247112146	0.0	0.0	18.931149137481107	0.0
35	0.0014993499247112146	0.0	0.0	19.385880550361378	0.0
36	0.0016421451556360921	0.0	0.0	19.826046849687316	0.0
37	0.0016421451556360921	0.0	0.0	20.246721599992004	0.0
38	0.001713542771098531	0.0	0.0	20.685102958931378	0.0
39	0.001713542771098531	0.0	0.0	21.127054198643872	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGAAAC	30	2.1658434E-6	45.000004	10
CTATACG	20	7.033815E-4	45.0	26
TATATCG	20	7.033815E-4	45.0	1
TACGTAG	20	7.033815E-4	45.0	1
CGCTTAA	20	7.033815E-4	45.0	44
TTAGCCG	55	6.184564E-11	40.909092	1
TAGAACG	55	6.184564E-11	40.909092	1
TACGGGT	160	0.0	39.375	4
CCCTCGT	535	0.0	38.69159	14
CGTGTAC	535	0.0	38.69159	18
CGAAACA	35	6.2492672E-6	38.57143	11
CTATCGG	35	6.2492672E-6	38.57143	2
CGATTAC	35	6.2492672E-6	38.57143	10
ACCGTAG	35	6.2492672E-6	38.57143	1
TGTAACG	70	0.0	38.57143	1
TCTATCG	35	6.2492672E-6	38.57143	1
AGGGTAC	510	0.0	38.382355	6
ATTACGG	60	1.5643309E-10	37.500004	2
CCAATCG	30	1.14004906E-4	37.500004	1
ACTTCGA	30	1.14004906E-4	37.500004	37
>>END_MODULE