##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552362_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	410579
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.164401978669147	28.0	23.0	31.0	16.0	33.0
2	27.083930254591685	30.0	25.0	31.0	16.0	33.0
3	27.211033686574325	30.0	25.0	33.0	16.0	34.0
4	30.63609195794232	33.0	28.0	35.0	19.0	37.0
5	32.451554999159725	33.0	32.0	35.0	28.0	37.0
6	31.475323871897977	35.0	31.0	35.0	17.0	37.0
7	33.21360079302643	35.0	32.0	35.0	28.0	37.0
8	33.53030232914981	35.0	33.0	37.0	28.0	37.0
9	35.08466823680705	37.0	34.0	39.0	30.0	39.0
10	35.16485499745481	37.0	34.0	39.0	30.0	39.0
11	35.48709748915556	37.0	34.0	39.0	30.0	39.0
12	35.20303035469423	37.0	34.0	39.0	30.0	39.0
13	35.346846770049126	37.0	34.0	39.0	30.0	39.0
14	35.89550610235789	38.0	34.0	40.0	29.0	41.0
15	36.15501767016823	38.0	34.0	40.0	30.0	41.0
16	35.80826345234413	38.0	34.0	40.0	28.0	41.0
17	35.730867871956434	37.0	34.0	40.0	30.0	41.0
18	35.452288110205345	37.0	34.0	39.0	27.0	40.0
19	34.77124986908731	37.0	33.0	39.0	27.0	40.0
20	35.01021484294131	36.0	34.0	39.0	29.0	40.0
21	35.173055611709316	37.0	34.0	39.0	29.0	40.0
22	35.718855567381674	37.0	34.0	39.0	30.0	40.0
23	36.184931523531404	38.0	35.0	40.0	31.0	41.0
24	36.16889806833764	38.0	35.0	40.0	31.0	41.0
25	34.44991097937303	36.0	33.0	39.0	25.0	40.0
26	34.951380854841574	36.0	33.0	39.0	27.0	40.0
27	35.56635629196817	37.0	34.0	39.0	30.0	41.0
28	35.606760209362875	37.0	34.0	40.0	30.0	41.0
29	35.74819462271572	38.0	35.0	40.0	30.0	41.0
30	35.01397051480957	37.0	34.0	40.0	27.0	41.0
31	34.99283207373003	37.0	34.0	40.0	27.0	41.0
32	34.74863302799218	37.0	34.0	40.0	25.0	41.0
33	34.64083647726747	38.0	34.0	40.0	23.0	41.0
34	34.444803557902375	38.0	34.0	40.0	22.0	41.0
35	34.272386069428784	38.0	34.0	40.0	20.0	41.0
36	33.99330701277951	38.0	33.0	40.0	18.0	41.0
37	34.08530636004277	38.0	34.0	40.0	18.0	41.0
38	33.72233845374459	37.0	33.0	40.0	18.0	41.0
39	33.68267251856525	37.0	33.0	40.0	18.0	41.0
40	33.49836206917548	37.0	33.0	40.0	18.0	41.0
41	33.25417520136198	36.0	33.0	40.0	18.0	41.0
42	33.489959301376835	37.0	33.0	40.0	18.0	41.0
43	33.56575470250549	37.0	33.0	40.0	17.0	41.0
44	33.68215373898811	37.0	33.0	40.0	18.0	41.0
45	33.67962316630904	37.0	33.0	40.0	18.0	41.0
46	33.54120400702423	36.0	33.0	40.0	18.0	41.0
47	33.450478470647546	36.0	33.0	40.0	18.0	41.0
48	33.50392494501667	36.0	33.0	40.0	18.0	41.0
49	33.57118849234861	36.0	33.0	40.0	18.0	41.0
50	33.44790892861057	36.0	33.0	40.0	18.0	41.0
51	32.390392591925064	35.0	31.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	14.0
9	27.0
10	36.0
11	35.0
12	37.0
13	29.0
14	61.0
15	71.0
16	135.0
17	234.0
18	484.0
19	754.0
20	1337.0
21	2100.0
22	3015.0
23	4117.0
24	5717.0
25	8081.0
26	10523.0
27	11624.0
28	11448.0
29	11939.0
30	13697.0
31	17723.0
32	23628.0
33	31170.0
34	37510.0
35	47513.0
36	57300.0
37	63519.0
38	40968.0
39	5730.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.20414828814918	15.767976443023146	18.856785174107785	26.171090094719894
2	37.7408488987503	24.98593449738053	21.22953195365569	16.04368465021348
3	27.042055243936	24.487613833147822	33.563333731145526	14.906997191770646
4	25.01686642521902	20.251157511709074	38.79156021131134	15.940415851760562
5	26.778281402604616	22.946619286422347	32.36234683215654	17.9127524788165
6	21.57489788810436	32.2276589888913	32.82900489309	13.36843822991434
7	64.71422064937565	4.9038065755920295	26.226864988223948	4.155107786808386
8	64.795569184006	9.581834433811762	20.488383477966483	5.1342129042157545
9	60.144332759347165	5.323944965524296	21.376641279753713	13.155080995374824
10	39.21169860124361	18.05036302392475	28.429364385416694	14.308573989414949
11	31.009622995818102	19.0489528202855	32.22571052099596	17.71571366290044
12	24.876820295241597	17.398356954447255	36.6638332696022	21.06098948070895
13	23.641491649597278	18.468552945961676	40.24219455939052	17.647760845050524
14	19.5796667632782	23.70773955803877	36.60537923274205	20.107214445940976
15	18.144133041387896	19.19265232756668	43.14248902160121	19.520725609444224
16	20.882704668285516	19.912124097920252	36.261231090727975	22.943940143066254
17	20.39169075866033	20.937261769354983	38.00705832495086	20.663989147033824
18	21.7561054023708	19.793998231765386	37.19552144654257	21.25437491932125
19	21.042479035703238	22.393010845659422	34.574832127312895	21.989677991324445
20	23.324622058117924	21.356182366852664	38.195085476850984	17.124110098178427
21	22.53792814537519	24.611828661475624	35.15328353374137	17.696959659407813
22	20.597984797079246	19.16245107518894	37.85459071214066	22.38497341559115
23	21.808714035544927	23.162899222804867	35.83622153105736	19.192165210592847
24	22.624634966717732	20.47182150085611	35.240964589031584	21.66257894339457
25	20.26211764362035	23.945452641270013	34.25528339247745	21.537146322632186
26	18.989524549477686	19.56968086531459	38.10959644794303	23.331198137264693
27	21.358374393234918	20.92703231290446	35.02736379600515	22.68722949785547
28	17.321879589555238	23.174833588663816	38.31564692787502	21.187639893905924
29	21.027865526488203	19.82614795203846	37.76520474744203	21.380781774031306
30	20.044132797829405	21.97335957270099	36.58930437260551	21.393203256864087
31	22.769065149459667	21.587806487910974	33.67780621999664	21.965322142632722
32	24.30324005855146	22.212534006853733	34.622569590748675	18.86165634384613
33	22.123635159128938	21.20590678042472	31.424890216012024	25.245567844434326
34	21.98139700276926	22.30338132247387	35.96433329517584	19.75088837958103
35	22.910329071871676	22.329685639060937	31.264872290107387	23.495112998960003
36	23.02260953434053	26.41927619288858	30.813314855362794	19.7447994174081
37	21.566129782575338	23.87019306881258	33.13418367719733	21.42949347141476
38	22.18281987144983	26.355707427803175	28.21649426785101	23.244978432895984
39	23.045260473623834	22.82191734112071	32.9405546800981	21.19226750515735
40	23.277615270142896	21.34668358586289	31.44948962319066	23.926211520803548
41	21.945350346705506	24.130557091327127	31.18985627613687	22.734236285830498
42	22.967078199323396	22.785870685056956	32.23691421139415	22.010136904225497
43	24.210200716549068	22.115841287547585	29.856130001777974	23.81782799412537
44	21.676461777148855	22.188665275135843	31.961206004203817	24.173666943511478
45	21.48526836491881	21.6050991404821	31.21299433239401	25.696638162205083
46	24.715584576902376	23.273474775865303	31.052489289515538	20.958451357716783
47	19.564322578602415	22.22227634633043	36.55398839200251	21.659412683064648
48	21.47357755754678	23.08837032580819	32.12390307346455	23.314149043180485
49	21.567347575009926	20.551952243051886	35.621402945596344	22.259297236341848
50	21.781435485010192	20.57021912957068	33.85828305880232	23.790062326616802
51	20.235813327033288	20.608701370503606	32.70893055903979	26.446554743423313
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	111.0
1	424.0
2	737.0
3	9579.0
4	18421.0
5	12154.5
6	5888.0
7	5529.5
8	5171.0
9	5000.5
10	4830.0
11	4620.0
12	4410.0
13	4159.5
14	3909.0
15	3625.5
16	3342.0
17	3134.5
18	2927.0
19	2686.0
20	2445.0
21	2437.0
22	2429.0
23	2263.5
24	2098.0
25	2162.0
26	2389.0
27	2552.0
28	3022.0
29	3492.0
30	3913.0
31	4334.0
32	4980.0
33	5626.0
34	6494.0
35	7362.0
36	7979.5
37	8597.0
38	8797.5
39	8998.0
40	10070.5
41	11143.0
42	12526.0
43	13909.0
44	15980.0
45	18051.0
46	32791.0
47	47531.0
48	38548.0
49	29565.0
50	29842.5
51	30120.0
52	26906.0
53	23692.0
54	22715.0
55	21738.0
56	20942.5
57	20147.0
58	19711.5
59	19276.0
60	18669.0
61	18062.0
62	17332.0
63	16602.0
64	14553.5
65	12505.0
66	10748.0
67	8991.0
68	7726.5
69	6462.0
70	5384.0
71	4306.0
72	3806.5
73	3307.0
74	2783.5
75	1874.0
76	1488.0
77	1130.5
78	773.0
79	601.5
80	430.0
81	312.5
82	195.0
83	131.5
84	68.0
85	47.5
86	27.0
87	23.5
88	20.0
89	13.0
90	6.0
91	3.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	410579.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.281604107542464
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.7087794088654	29.739623002043125
2	7.651697610868044	6.011419126014939
3	3.2997048257378765	3.888530959091479
4	2.1526432587563207	3.3823712110094344
5	1.714147737986986	3.3667236412722104
6	1.4292356257847325	3.368560081708294
7	1.2123760843327733	3.333685416194876
8	1.0528193502922796	3.3085146331953115
9	0.9296992859476237	3.2868071360693523
>10	4.7671359288706885	27.56405677510741
>50	0.0530509543310759	1.3981921549943908
>100	0.021844510606913607	1.6607669047750313
>500	0.001248257748966492	0.3689775895610307
>1k	0.00312064437241623	1.8809276193436728
>5k	0.0018723866234497378	4.838877531671803
>10k+	6.24128874483246E-4	2.6019662179476537
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10613	2.5848862216528365	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGC	6868	1.6727596881477136	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCG	6457	1.5726571500247213	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTC	6412	1.5616970181134446	No Hit
GCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCTGC	2279	0.5550697916844262	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCATCTGGT	1643	0.4001665940050514	No Hit
GAACTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCT	1300	0.31662603299243264	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCATCTGGTCGTA	1275	0.3105370708195012	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCT	1175	0.28618122212777564	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTC	899	0.218959079738613	No Hit
CCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCTGC	606	0.14759644307185707	No Hit
CTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCTGCT	485	0.1181258661548691	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1220228019455452	0.0
2	0.0	0.0	0.0	0.9467118386473736	0.0
3	0.0	0.0	0.0	1.1639660089775659	0.0
4	0.0	0.0	0.0	1.7231762949395852	0.0
5	0.0	0.0	0.0	3.91593335265564	0.0
6	0.0	0.0	0.0	4.42448347333887	0.0
7	0.0	0.0	0.0	5.120086511974553	0.0
8	0.0	0.0	0.0	5.888757096685413	0.0
9	0.0	0.0	0.0	6.176156111247774	0.0
10	0.0	0.0	0.0	8.268079955380085	0.0
11	0.0	0.0	0.0	8.973668891979376	0.0
12	0.0	0.0	0.0	11.318406445531798	0.0
13	0.0	0.0	0.0	11.677168096760916	0.0
14	0.0	0.0	0.0	11.847902596089913	0.0
15	0.0	0.0	0.0	12.36375947138066	0.0
16	0.0	0.0	0.0	12.791691732894279	0.0
17	0.0	0.0	0.0	13.266387223896011	0.0
18	0.0	0.0	0.0	13.757157575034281	0.0
19	0.0	0.0	0.0	14.477603579335524	0.0
20	0.0	0.0	0.0	14.84099284181607	0.0
21	0.0	0.0	0.0	15.180269814091806	0.0
22	0.0	0.0	0.0	15.585064019348286	0.0
23	0.0	0.0	0.0	15.928237927414699	0.0
24	0.0	0.0	0.0	16.221482345663077	0.0
25	0.0	0.0	0.0	16.473808938109354	0.0
26	0.0	0.0	0.0	16.725891972068712	0.0
27	0.0	0.0	0.0	17.00744558294506	0.0
28	0.0	0.0	0.0	17.2380954700557	0.0
29	0.0	0.0	0.0	17.47702634572153	0.0
30	0.0	0.0	0.0	17.740313070079083	0.0
31	0.0	0.0	0.0	17.972667866598144	0.0
32	0.0	0.0	0.0	18.243261345563216	0.0
33	0.0	0.0	0.0	18.508983654789944	0.0
34	2.4355848691725587E-4	0.0	0.0	18.781282043163436	0.0
35	2.4355848691725587E-4	0.0	0.0	19.07744916325482	0.0
36	2.4355848691725587E-4	0.0	0.0	19.34804264221989	0.0
37	4.8711697383451173E-4	0.0	0.0	19.60621463835218	0.0
38	4.8711697383451173E-4	0.0	0.0	19.9113934224595	0.0
39	4.8711697383451173E-4	0.0	0.0	20.23264706670336	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGACGG	60	0.0	45.000004	2
TATGGGT	20	7.027983E-4	45.0	4
CTGAACG	20	7.027983E-4	45.0	1
CGAATGC	20	7.027983E-4	45.0	45
TTAGTCA	55	1.8189894E-12	45.0	32
TATAGAG	20	7.027983E-4	45.0	1
CGGTCGA	20	7.027983E-4	45.0	41
CGTCATA	20	7.027983E-4	45.0	38
GTAACGG	20	7.027983E-4	45.0	2
ACGAACG	20	7.027983E-4	45.0	1
CGTTTTT	5465	0.0	43.84721	1
ATAGGGC	80	0.0	42.1875	4
GCGTTAG	60	3.6379788E-12	41.250004	1
CCGATGA	475	0.0	41.21053	18
TACGGCT	755	0.0	41.125828	7
ACGGCTG	765	0.0	40.588234	8
TACGGGA	100	0.0	40.500004	4
GATGAAT	435	0.0	40.344826	20
CGATGAA	430	0.0	40.2907	19
AACGGGC	95	0.0	40.263157	4
>>END_MODULE