FastQCFastQC Report
Sat 18 Jun 2016
SRR3552361_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552361_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences768568
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154052.0043769711983845No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCG129461.684431306013261No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTC126911.6512527193429858No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGC114971.4958988664633448No Hit
GCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC40920.5324187319794735TruSeq Adapter, Index 14 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCT21970.2858562937827232No Hit
GAACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCT21300.2771387827752392No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTC19230.2502055771252511No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTA12260.15951744022649914No Hit
CCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC9920.12907120775259964TruSeq Adapter, Index 14 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATA253.8895945E-545.00000424
CAATACG253.8895945E-545.0000041
TGCGTCG302.1645592E-645.0000041
CGTATCT207.031826E-445.022
TACGATC207.031826E-445.011
CAATGCG207.031826E-445.01
ATACGAT207.031826E-445.010
CGTTTTT84900.043.674911
TACGGCT15400.041.639617
CGGTCTA603.6379788E-1241.25000431
CGAATAT501.0804797E-940.50000414
CACGACC1800.040.027
ACGGCTG16100.039.8291938
TGATACC19550.038.7851684
TATACGG356.2455892E-638.5714262
GATACCT19800.038.1818165
GAATGAT46200.038.1818161
GAATCTG20250.038.111111
AATGACT3400.037.720592
CGGCTGT16950.037.5663729