FastQCFastQC Report
Sat 18 Jun 2016
SRR3552359_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552359_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465903
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88631.9023273084740815No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTC73751.5829475234115253No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCG62761.3470615128041674No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGC56381.21012313721955No Hit
GCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGC22420.4812160471171038No Hit
GAACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCT18840.4043760181840426No Hit
GAATGACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCT11480.2464032212713805No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTC10440.22408097822937392No Hit
CCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGC7330.1573288860556811No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTA6690.14359212110675398No Hit
CTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGCT5860.1257772540636141Illumina Single End Adapter 1 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTT5010.10753311311582024No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAC302.162713E-645.0000049
TTCACGG351.2098462E-745.02
GCGTTAG253.8872233E-545.01
ATCCGCA207.028964E-445.011
CATAGCG207.028964E-445.01
CATACGA253.8872233E-545.018
TGTAGCG207.028964E-445.01
CGATATA207.028964E-445.010
CGTTTTT39450.043.5741461
TACGGCT7150.039.9650347
ACGGCTG7350.038.8775528
CGAGACA2000.038.2522
CGGGTAT301.13888E-437.5000046
GTTAGCG301.13888E-437.5000041
CGTTAGG601.546141E-1037.5000042
ACATACG301.13888E-437.50000417
AGTACGG601.546141E-1037.5000042
TGCGTAG301.13888E-437.5000041
GGCGATA1800.037.58
CACGACC2000.037.12527