##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552358_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	356424
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.637173703229863	28.0	16.0	31.0	16.0	33.0
2	26.69713038403699	30.0	25.0	31.0	16.0	33.0
3	26.907194801696857	30.0	25.0	33.0	16.0	34.0
4	30.764432249231252	33.0	28.0	35.0	19.0	37.0
5	32.355820595695015	35.0	32.0	35.0	28.0	37.0
6	31.275579085583463	35.0	30.0	35.0	17.0	37.0
7	33.14408962359437	35.0	32.0	35.0	28.0	37.0
8	33.6262541243014	35.0	35.0	37.0	30.0	37.0
9	35.07047505218504	37.0	34.0	39.0	29.0	39.0
10	35.153202365721725	37.0	34.0	39.0	30.0	39.0
11	35.501004421699996	37.0	34.0	39.0	30.0	39.0
12	35.24919477925168	37.0	34.0	39.0	30.0	39.0
13	35.43997317801271	37.0	34.0	39.0	30.0	39.0
14	35.901990326128434	38.0	34.0	40.0	30.0	41.0
15	36.17454772967028	38.0	34.0	40.0	30.0	41.0
16	35.77215058469688	38.0	34.0	40.0	27.0	41.0
17	35.78299721679797	37.0	34.0	40.0	30.0	41.0
18	35.424135299530896	37.0	34.0	39.0	27.0	40.0
19	34.782983188561936	37.0	32.0	39.0	27.0	40.0
20	35.32689717864117	37.0	34.0	39.0	30.0	40.0
21	35.36999472538325	37.0	34.0	39.0	29.0	40.0
22	36.04315365968622	38.0	34.0	40.0	30.0	40.0
23	36.50555518146926	38.0	35.0	40.0	31.0	41.0
24	36.52870738221893	38.0	35.0	40.0	31.0	41.0
25	34.56746459273225	37.0	33.0	39.0	25.0	41.0
26	35.31341043251857	37.0	34.0	39.0	29.0	41.0
27	35.93141034273786	38.0	35.0	40.0	30.0	41.0
28	36.06081801449958	38.0	35.0	40.0	30.0	41.0
29	36.10182815971989	38.0	35.0	40.0	30.0	41.0
30	35.32410836531771	37.0	34.0	40.0	27.0	41.0
31	35.438797611833095	37.0	34.0	40.0	29.0	41.0
32	35.33989013085539	38.0	34.0	40.0	28.0	41.0
33	35.43255224115099	38.0	35.0	40.0	27.0	41.0
34	35.31741689672974	38.0	35.0	40.0	26.0	41.0
35	35.19805063632079	38.0	35.0	40.0	25.0	41.0
36	34.883119543016186	38.0	34.0	40.0	24.0	41.0
37	35.02364599465805	38.0	34.0	40.0	25.0	41.0
38	34.748580342513414	38.0	34.0	40.0	24.0	41.0
39	34.712712948622986	38.0	34.0	40.0	23.0	41.0
40	34.55988092833255	38.0	34.0	40.0	23.0	41.0
41	34.26550681211142	37.0	33.0	40.0	23.0	41.0
42	34.573555652817994	38.0	34.0	40.0	23.0	41.0
43	34.526207550557764	37.0	34.0	40.0	23.0	41.0
44	34.692733934864094	38.0	34.0	40.0	23.0	41.0
45	34.71691580813862	37.0	34.0	40.0	23.0	41.0
46	34.515529257289074	37.0	34.0	40.0	23.0	41.0
47	34.46313940699841	37.0	34.0	40.0	23.0	41.0
48	34.473567997665704	37.0	34.0	40.0	23.0	41.0
49	34.5347703858326	37.0	34.0	40.0	24.0	41.0
50	34.37732027023994	37.0	34.0	40.0	23.0	41.0
51	33.336228761250645	35.0	32.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	4.0
8	11.0
9	23.0
10	31.0
11	23.0
12	20.0
13	39.0
14	48.0
15	65.0
16	124.0
17	189.0
18	342.0
19	563.0
20	848.0
21	1314.0
22	1989.0
23	2693.0
24	3866.0
25	5566.0
26	6933.0
27	7731.0
28	8182.0
29	9100.0
30	11253.0
31	14600.0
32	19711.0
33	26553.0
34	32885.0
35	43508.0
36	54476.0
37	60625.0
38	38079.0
39	5029.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.4037775233991	16.607467510605346	19.47960855610172	22.509146409893834
2	35.334320921150095	24.54492402307364	22.998451282741904	17.122303773034364
3	29.852086279262902	23.319697887908784	30.906167934819205	15.922047898009112
4	26.12310057684107	22.485298408636904	34.03783134693511	17.353769667586917
5	26.681704935694565	25.108017417457862	29.531400803537363	18.678876843310217
6	23.622427221511458	31.589623594370746	29.964873296972144	14.823075887145649
7	69.45127151931408	5.274616748591566	20.62122640450699	4.652885327587368
8	69.53740488855969	8.072969272551793	16.748030435660898	5.641595403227616
9	63.916010145220305	6.152503759567257	18.382319933562275	11.549166161650168
10	38.07207146544565	21.96933988732521	24.75029739860391	15.208291248625233
11	30.125636881915916	22.368864049558955	29.772405898592684	17.73309316993244
12	25.11278701770924	19.681334590263283	34.30577065517473	20.90010773685274
13	23.911970013242655	20.498339056853634	37.44977891500012	18.139912014903597
14	20.841189145512086	24.68829259533589	33.241308104953646	21.22921015419837
15	19.38365542163266	21.77883644199044	38.80827329248311	20.02923484389379
16	22.259443808497746	22.100363611877988	33.37738199447849	22.262810585145782
17	21.66099925930914	21.98925998249276	34.779925033106636	21.569815725091466
18	22.227459430341394	21.780519830314457	33.79486229883509	22.197158440509057
19	21.695228155230847	24.229849841761496	31.879446950822615	22.19547505218504
20	24.439431688101813	23.139294772518124	34.339157856934435	18.082115682445625
21	23.729602944807308	25.376517855138825	31.883374856911995	19.010504343141875
22	21.982526429196685	21.352658631293068	33.76568356788544	22.899131371624808
23	21.94408906246493	24.85354521581038	33.40094943101475	19.80141629070994
24	22.93897156195991	21.78669225416919	32.61031804816735	22.664018135703547
25	21.162155185958298	25.0241285659776	31.21030009202523	22.603416156038875
26	20.606637039032165	22.83432092115009	33.23457455165757	23.32446748816017
27	22.3368796714026	23.852770857181333	31.394631113505262	22.4157183579108
28	19.023410320292687	24.637790945615336	34.40368774268848	21.935110991403498
29	22.62025003927906	24.69109824254259	31.19514959710906	21.493502121069287
30	22.330707247547863	25.272147779049668	32.777534621686534	19.619610351715934
31	24.140630260588512	23.30763360491998	31.000998810405584	21.55073732408592
32	26.67693533544318	24.778353870670884	29.563104616973046	18.98160617691289
33	25.175633515139275	22.972919893160952	29.566751958341754	22.284694633358022
34	25.73592126231679	24.46215743047606	30.555181469261328	19.246739837945817
35	24.586728166453437	24.848214486117655	29.09287814513052	21.472179202298385
36	23.2851884272664	27.26864633133571	30.37618117747402	19.06998406392387
37	24.87963773483267	26.08017417457859	30.109925257558412	18.930262833030323
38	24.8459699683523	24.826891567346756	29.287309496554663	21.03982896774628
39	27.53995241622337	23.19260206944538	28.942495454851525	20.324950059479722
40	27.426323704352118	22.60061050883218	29.98395169797769	19.989114088838015
41	23.200177316903464	24.793784930307723	29.881265010212555	22.12477274257626
42	24.26463986712455	24.00006733553296	30.54003097434516	21.19526182299733
43	23.683309765896798	23.24955670774134	30.38824546046282	22.67888806589904
44	23.468677754584427	23.22683096536709	30.41012950867506	22.89436177137342
45	23.817700267097614	22.0139496779117	29.396729737615875	24.77162031737481
46	25.93063317846161	24.15550019078401	28.86309563890198	21.0507709918524
47	21.625367539784076	23.08374295782551	33.94356160079007	21.34732790160034
48	22.867988687630465	23.39825600969632	30.71987296029448	23.01388234237874
49	23.165106726819744	21.855711175453955	33.2881624133055	21.6910196844208
50	22.393553744977893	22.430868852826972	31.570825758085874	23.60475164410926
51	22.0380782438893	21.618353421767335	29.972729109150897	26.370839225192466
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	82.0
1	333.0
2	584.0
3	5961.0
4	11338.0
5	7392.0
6	3446.0
7	3266.0
8	3086.0
9	2989.5
10	2893.0
11	2781.0
12	2669.0
13	2498.0
14	2327.0
15	2215.0
16	2103.0
17	1984.5
18	1866.0
19	1780.0
20	1694.0
21	1748.0
22	1802.0
23	1784.5
24	1767.0
25	1958.0
26	2488.5
27	2828.0
28	3096.0
29	3364.0
30	4044.0
31	4724.0
32	5020.0
33	5316.0
34	5920.5
35	6525.0
36	7413.0
37	8301.0
38	8980.0
39	9659.0
40	10516.5
41	11374.0
42	12682.0
43	13990.0
44	15763.5
45	17537.0
46	28346.0
47	39155.0
48	33461.5
49	27768.0
50	27771.0
51	27774.0
52	25016.5
53	22259.0
54	20843.0
55	19427.0
56	18217.0
57	17007.0
58	16801.0
59	16595.0
60	15870.5
61	15146.0
62	14194.5
63	13243.0
64	11703.0
65	10163.0
66	8939.0
67	7715.0
68	6582.0
69	5449.0
70	4820.5
71	4192.0
72	3631.5
73	3071.0
74	2635.5
75	1970.0
76	1740.0
77	1340.5
78	941.0
79	774.5
80	608.0
81	462.0
82	316.0
83	220.0
84	124.0
85	91.0
86	58.0
87	40.5
88	23.0
89	22.5
90	22.0
91	11.0
92	0.0
93	1.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	356424.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.1196607094916
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.6939687779389	31.039649612626537
2	8.320862845200558	7.009438397001248
3	3.8461940905621	4.860011703519818
4	2.7542990484015357	4.64040565644593
5	2.174450709708142	4.5793563061210065
6	1.7526455141600756	4.429250064026089
7	1.4616014134316808	4.309350893837903
8	1.2512211527665205	4.216080834165184
9	1.023395870138238	3.8794578139545752
>10	3.660237022244096	20.964183371824028
>50	0.036942808237890194	1.0496052391706578
>100	0.01679218556267736	1.2855542336365144
>500	0.003358437112535472	1.0761990107291597
>1k	0.003358437112535472	4.633257412910475
>5k	6.716874225070944E-4	2.0281994500308476
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7169	2.0113684824815388	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTC	4506	1.264224631337957	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGC	4368	1.2255066998855295	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCG	4207	1.1803357798576977	No Hit
GCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGC	1858	0.5212892510044217	No Hit
GAACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCT	1438	0.40345206832312075	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATGGGGTT	937	0.26288914326756896	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTA	803	0.22529347069782057	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCT	728	0.20425111664758824	No Hit
CCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGC	716	0.20088433999955108	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTC	620	0.17395012681525374	No Hit
CTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGCT	458	0.12849864206675196	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13719614840751465	0.0
2	0.0	0.0	0.0	1.0167665477072252	0.0
3	0.0	0.0	0.0	1.2942450564496217	0.0
4	0.0	0.0	0.0	2.019224294660292	0.0
5	0.0	0.0	0.0	4.094842098175207	0.0
6	0.0	0.0	0.0	4.813087783089803	0.0
7	0.0	0.0	0.0	5.614380625322649	0.0
8	0.0	0.0	0.0	6.583731735236684	0.0
9	0.0	0.0	0.0	6.972875002805647	0.0
10	0.0	0.0	0.0	8.879031715036025	0.0
11	0.0	0.0	0.0	9.691266581374991	0.0
12	0.0	0.0	0.0	11.709649181873274	0.0
13	0.0	0.0	0.0	12.110295602989698	0.0
14	0.0	0.0	0.0	12.31005768410657	0.0
15	0.0	0.0	0.0	12.776917379301057	0.0
16	0.0	0.0	0.0	13.254719098601665	0.0
17	0.0	0.0	0.0	13.781619644019482	0.0
18	0.0	0.0	0.0	14.33040423764954	0.0
19	0.0	0.0	0.0	14.961394294435841	0.0
20	0.0	0.0	0.0	15.35586829169753	0.0
21	0.0	0.0	0.0	15.750622853679888	0.0
22	0.0	0.0	0.0	16.194756806500124	0.0
23	0.0	0.0	0.0	16.57884990909703	0.0
24	0.0	0.0	0.0	16.917210962224765	0.0
25	0.0	0.0	0.0	17.20002020065989	0.0
26	0.0	0.0	0.0	17.485915651022378	0.0
27	0.0	0.0	0.0	17.796781361524477	0.0
28	0.0	0.0	0.0	18.071173658339507	0.0
29	0.0	0.0	0.0	18.359594191188023	0.0
30	0.0	0.0	0.0	18.692063385181694	0.0
31	0.0	0.0	0.0	19.001245707359775	0.0
32	0.0	0.0	0.0	19.309025205934503	0.0
33	0.0	0.0	0.0	19.608107198168472	0.0
34	0.0	0.0	0.0	19.90803088456445	0.0
35	0.0	0.0	0.0	20.228996925010662	0.0
36	0.0	0.0	0.0	20.511806163445783	0.0
37	0.0	0.0	0.0	20.822391309227214	0.0
38	0.0	0.0	0.0	21.126242901712565	0.0
39	0.0	0.0	0.0	21.45758983682356	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	20	7.0267305E-4	45.0	28
CGACGAA	20	7.0267305E-4	45.0	19
CGCATCG	20	7.0267305E-4	45.0	21
TTATGCG	20	7.0267305E-4	45.0	1
ATCGTAT	35	1.208773E-7	45.0	36
CATACGA	35	1.208773E-7	45.0	18
CGTAAGG	20	7.0267305E-4	45.0	2
ATAACGC	20	7.0267305E-4	45.0	11
ACATACG	35	1.208773E-7	45.0	17
TAGTCCG	20	7.0267305E-4	45.0	2
CGTTTTT	3190	0.0	43.800938	1
ACGGGAT	55	6.002665E-11	40.909092	5
TACGGCT	565	0.0	40.61947	7
GGCGTAG	40	3.4492768E-7	39.375	1
CGTTAGG	80	0.0	39.375	2
TACGAAA	40	3.4492768E-7	39.375	20
ATACGAA	40	3.4492768E-7	39.375	19
CCCATTA	40	3.4492768E-7	39.375	33
TACGGGA	70	0.0	38.571426	4
CGATGAA	260	0.0	38.076927	19
>>END_MODULE