Basic Statistics
Measure | Value |
---|---|
Filename | SRR3552357_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 562717 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 45 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 17596 | 3.126971461676118 | No Hit |
GAATGATACCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCG | 14933 | 2.653731804797083 | No Hit |
GAATCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTC | 14924 | 2.6521324218035 | No Hit |
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGC | 13438 | 2.3880565186408087 | No Hit |
GCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC | 3744 | 0.6653433253304948 | TruSeq Adapter, Index 16 (95% over 21bp) |
GAATGACTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCT | 2531 | 0.449782039639819 | No Hit |
GAACTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCT | 2480 | 0.4407188693428491 | No Hit |
GAATGATCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTC | 2221 | 0.3946921809719628 | No Hit |
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACTAGCACTCGTA | 1655 | 0.29410876159774807 | No Hit |
GAATGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTT | 1172 | 0.20827520760879803 | No Hit |
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 714 | 0.12688438415757833 | No Hit |
CTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGCT | 678 | 0.12048685218324663 | TruSeq Adapter, Index 13 (95% over 22bp) |
CCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC | 573 | 0.10182738392477925 | TruSeq Adapter, Index 16 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTGCCA | 25 | 3.888258E-5 | 45.000004 | 42 |
ATTACGG | 20 | 7.030212E-4 | 45.000004 | 2 |
TAGCCGT | 20 | 7.030212E-4 | 45.000004 | 44 |
GTATGCG | 20 | 7.030212E-4 | 45.000004 | 1 |
TATCGGG | 25 | 3.888258E-5 | 45.000004 | 3 |
CGATAGA | 25 | 3.888258E-5 | 45.000004 | 10 |
CGTTTTT | 7035 | 0.0 | 44.00853 | 1 |
TACGGCT | 1610 | 0.0 | 42.624226 | 7 |
AACACGT | 355 | 0.0 | 41.830986 | 41 |
TCAAGCG | 395 | 0.0 | 41.58228 | 17 |
ACGGCTG | 1675 | 0.0 | 40.97015 | 8 |
ACCGCGC | 55 | 6.002665E-11 | 40.909092 | 35 |
GACACGA | 355 | 0.0 | 40.56338 | 25 |
GCGTAAG | 50 | 1.0786607E-9 | 40.500004 | 1 |
GAATCTG | 2035 | 0.0 | 40.2457 | 1 |
CGAGACA | 370 | 0.0 | 40.135136 | 22 |
GTCAAGC | 415 | 0.0 | 40.120483 | 16 |
CACGACC | 365 | 0.0 | 40.068493 | 27 |
ACACGAC | 360 | 0.0 | 40.0 | 26 |
CGTAAGG | 90 | 0.0 | 40.0 | 2 |