FastQCFastQC Report
Sat 18 Jun 2016
SRR3552357_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552357_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences562717
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175963.126971461676118No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCG149332.653731804797083No Hit
GAATCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTC149242.6521324218035No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGC134382.3880565186408087No Hit
GCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC37440.6653433253304948TruSeq Adapter, Index 16 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCT25310.449782039639819No Hit
GAACTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCT24800.4407188693428491No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTC22210.3946921809719628No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACTAGCACTCGTA16550.29410876159774807No Hit
GAATGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTT11720.20827520760879803No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7140.12688438415757833No Hit
CTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGCT6780.12048685218324663TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC5730.10182738392477925TruSeq Adapter, Index 16 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCCA253.888258E-545.00000442
ATTACGG207.030212E-445.0000042
TAGCCGT207.030212E-445.00000444
GTATGCG207.030212E-445.0000041
TATCGGG253.888258E-545.0000043
CGATAGA253.888258E-545.00000410
CGTTTTT70350.044.008531
TACGGCT16100.042.6242267
AACACGT3550.041.83098641
TCAAGCG3950.041.5822817
ACGGCTG16750.040.970158
ACCGCGC556.002665E-1140.90909235
GACACGA3550.040.5633825
GCGTAAG501.0786607E-940.5000041
GAATCTG20350.040.24571
CGAGACA3700.040.13513622
GTCAAGC4150.040.12048316
CACGACC3650.040.06849327
ACACGAC3600.040.026
CGTAAGG900.040.02