##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552357_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	562717
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.085101391996332	30.0	28.0	31.0	16.0	33.0
2	28.698761544435303	31.0	28.0	31.0	16.0	33.0
3	28.244973938942664	31.0	27.0	31.0	16.0	34.0
4	32.026199670527106	35.0	32.0	35.0	25.0	37.0
5	33.270212202581405	35.0	32.0	35.0	28.0	37.0
6	33.29050837276997	35.0	33.0	35.0	28.0	37.0
7	34.02406360568456	35.0	33.0	35.0	30.0	37.0
8	34.014637908575715	35.0	35.0	37.0	30.0	37.0
9	35.35205796163969	37.0	34.0	39.0	30.0	39.0
10	35.39128904938006	37.0	34.0	39.0	30.0	39.0
11	35.683448340817854	37.0	35.0	39.0	30.0	39.0
12	35.67508001357699	37.0	35.0	39.0	30.0	39.0
13	35.588327702912835	37.0	35.0	39.0	30.0	39.0
14	36.83953745843826	38.0	36.0	40.0	31.0	41.0
15	36.75550765304762	38.0	35.0	40.0	31.0	41.0
16	36.70286840454438	38.0	35.0	40.0	31.0	41.0
17	35.75058866179625	38.0	33.0	40.0	29.0	41.0
18	35.37668845974086	37.0	33.0	39.0	27.0	40.0
19	34.76918415473497	37.0	33.0	39.0	27.0	40.0
20	35.12609357812186	36.0	34.0	39.0	29.0	40.0
21	35.65220172839989	37.0	34.0	39.0	30.0	40.0
22	36.08284093069874	38.0	35.0	40.0	31.0	41.0
23	36.27137975216672	38.0	35.0	40.0	31.0	41.0
24	36.18025224046901	38.0	35.0	40.0	31.0	41.0
25	35.533028147363595	37.0	34.0	40.0	29.0	41.0
26	35.61385563258263	37.0	34.0	40.0	30.0	41.0
27	35.9797358174713	38.0	35.0	40.0	30.0	41.0
28	35.851504397414686	38.0	35.0	40.0	30.0	41.0
29	35.79063365777114	38.0	35.0	40.0	30.0	41.0
30	35.3284510686544	37.0	34.0	40.0	27.0	41.0
31	35.36215895379027	38.0	34.0	40.0	28.0	41.0
32	35.10185759449244	37.0	34.0	40.0	27.0	41.0
33	34.8559453508602	38.0	34.0	40.0	24.0	41.0
34	34.528041626608044	38.0	34.0	40.0	22.0	41.0
35	34.22361684470169	38.0	34.0	40.0	20.0	41.0
36	33.95047243996538	38.0	33.0	40.0	18.0	41.0
37	33.92373253340489	38.0	33.0	40.0	18.0	41.0
38	33.747700886946724	37.0	33.0	40.0	18.0	41.0
39	33.54515324754717	37.0	33.0	40.0	17.0	41.0
40	33.324539688688986	37.0	33.0	40.0	15.0	41.0
41	33.249489530261215	37.0	33.0	40.0	15.0	41.0
42	33.13897038831242	37.0	33.0	40.0	15.0	41.0
43	33.13498614756618	37.0	33.0	40.0	15.0	41.0
44	32.98307675083568	36.0	33.0	40.0	15.0	41.0
45	32.95206649168232	36.0	33.0	40.0	15.0	40.0
46	32.6304199091195	36.0	32.0	39.0	15.0	40.0
47	32.56476879141736	35.0	32.0	39.0	15.0	40.0
48	32.54913037992454	35.0	32.0	39.0	15.0	40.0
49	32.47846963926805	35.0	32.0	39.0	13.0	40.0
50	32.33724412093468	35.0	31.0	39.0	12.0	40.0
51	29.78170732357473	34.0	26.0	37.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	13.0
9	32.0
10	38.0
11	23.0
12	27.0
13	32.0
14	47.0
15	100.0
16	218.0
17	439.0
18	841.0
19	1320.0
20	1893.0
21	2670.0
22	3799.0
23	5592.0
24	8037.0
25	11382.0
26	14957.0
27	16261.0
28	15721.0
29	15711.0
30	17789.0
31	21901.0
32	28722.0
33	38180.0
34	51232.0
35	61645.0
36	72314.0
37	85104.0
38	71202.0
39	15473.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.94645443446706	14.626712894758823	17.316697380743783	25.110135290030332
2	36.51800105559278	28.447514469973363	20.070124058807536	14.964360415626327
3	26.03120218511259	27.90105861383253	32.95031072457381	13.117428476481074
4	23.35792236594949	19.588887486960584	41.76930855119003	15.283881595899892
5	27.094081038959196	22.35848570418168	32.32886157695609	18.218571679903043
6	20.048265824561902	32.8479502129845	34.37589409952072	12.727889862932878
7	62.941052074133175	4.999493528718698	27.837083294089215	4.222371103058909
8	61.57073626707563	10.28723141472534	22.895878390025537	5.246153928173487
9	57.421048235613995	5.590376690236833	22.383364995192963	14.605210078956206
10	34.87543472118312	19.38878690354121	29.93689545544208	15.798882919833593
11	28.135457077003185	19.891704000412286	34.159621976944	17.813216945640526
12	22.996284100178244	18.255890616419975	38.802275388872204	19.945549894529577
13	19.860427177426665	19.686449849569144	44.304152886797446	16.14897008620674
14	19.53806264960895	23.82529761851872	36.44194150878683	20.194698223085496
15	16.85376485871228	19.69000403399933	46.02775462621531	17.42847648107308
16	19.63775752287562	21.245848268312493	35.90579278749353	23.21060142131835
17	18.74263617413371	19.449563457297362	40.04552910255066	21.762271266018267
18	20.55473710586316	20.50213517629643	37.930433948148	21.012693769692405
19	19.571294274031175	22.627715174768134	35.54219972028568	22.258790830915007
20	20.998299322750157	22.262167306123683	39.458555543905725	17.280977827220433
21	20.973597740960376	25.76499377129179	35.97083436256591	17.29057412518193
22	19.643088799520896	19.029281148428073	36.32056611049604	25.007063941554993
23	19.48190653561204	25.4488490662269	36.93615085380396	18.13309354435711
24	22.621139933572294	18.919989977199908	35.00178597767617	23.457084111551634
25	18.886402934334665	27.45767410616704	33.823040711405554	19.832882248092737
26	18.704073273066214	20.7599912567063	35.962837447598	24.57309802262949
27	21.59753481767922	21.915456614959204	33.15058901721469	23.33641955014688
28	16.99859787424229	21.818605089236687	39.54847640998939	21.63432062653163
29	21.122340359363587	24.036416173671668	33.043430356644635	21.797813110320106
30	20.308432124851393	21.615305739830145	36.25694620919574	21.819315926122723
31	22.25132704361162	20.804951689748133	34.88200996237896	22.061711304261287
32	22.406111775546144	27.202483664079814	31.706523883230826	18.684880677143216
33	22.06544319791298	19.889038362089646	30.85120940010698	27.194309039890392
34	21.19857761539104	25.208586198746442	32.00063264482858	21.592203541033946
35	18.8851589697841	26.34699147173446	29.15781822834569	25.61003133013575
36	20.066925292820372	26.585121828556808	30.716505810203	22.631447068419828
37	19.442099669993976	25.836077459895474	31.532546555373305	23.18927631473725
38	22.56054108903765	24.548396440839714	30.42506268692789	22.46599978319475
39	23.277597797827326	23.954136804112903	28.6083413154392	24.15992408262057
40	24.327859296946777	24.622678895430564	31.05895148005125	19.99051032757141
41	18.962817899583627	25.37136784564888	27.831396599000918	27.834417655766575
42	20.367964714056978	25.067662786089635	33.31123815345902	21.25313434639437
43	23.363431351816278	21.516854831114042	28.331825766770862	26.787888050298818
44	22.874197865001413	21.445948851731867	31.356436716857672	24.323416566409048
45	20.74630764665009	20.87994498122502	30.776038399408584	27.5977089727163
46	25.670807883891904	23.7689637953003	29.003033496411163	21.557194824396632
47	19.07299761691934	21.053389181418012	38.429974569810405	21.443638631852245
48	22.14967736890835	23.530478020034938	29.870254497376123	24.44959011368059
49	20.33988665705852	20.838716441834883	36.10091751271065	22.720479388395944
50	21.651203002575006	20.3530371394502	33.576913439615296	24.418846418359493
51	21.29294121201243	20.016455873911752	30.01508751290613	28.675515401169683
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	201.0
1	676.0
2	1151.0
3	14273.0
4	27395.0
5	18054.0
6	8713.0
7	7749.0
8	6785.0
9	6439.5
10	6094.0
11	5864.5
12	5635.0
13	5420.0
14	5205.0
15	4953.5
16	4702.0
17	4348.0
18	3994.0
19	3676.5
20	3359.0
21	3297.0
22	3235.0
23	3245.5
24	3256.0
25	3276.5
26	3645.5
27	3994.0
28	4492.0
29	4990.0
30	5525.5
31	6061.0
32	6880.5
33	7700.0
34	8532.0
35	9364.0
36	10169.0
37	10974.0
38	11771.5
39	12569.0
40	13872.0
41	15175.0
42	17264.5
43	19354.0
44	23076.5
45	26799.0
46	56123.0
47	85447.0
48	62499.5
49	39552.0
50	39519.5
51	39487.0
52	35339.0
53	31191.0
54	29116.5
55	27042.0
56	25278.0
57	23514.0
58	22456.0
59	21398.0
60	21393.0
61	21388.0
62	20123.5
63	18859.0
64	17088.5
65	15318.0
66	13175.0
67	11032.0
68	9745.0
69	8458.0
70	7343.0
71	6228.0
72	5709.0
73	5190.0
74	4334.0
75	2830.0
76	2182.0
77	1764.0
78	1346.0
79	1059.0
80	772.0
81	568.0
82	364.0
83	296.5
84	229.0
85	169.5
86	110.0
87	91.5
88	73.0
89	48.5
90	24.0
91	23.5
92	23.0
93	14.5
94	6.0
95	4.0
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	562717.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.72333673063413
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.76648502216024	27.003118457822854
2	7.0982620536520145	4.929506869824829
3	2.725717762829772	2.8393804713422677
4	1.7160603781728592	2.383493694455822
5	1.2675711563760619	2.2007150046442647
6	1.035235646950316	2.156810157876701
7	0.9067839800376721	2.2040595836554893
8	0.7985350518705886	2.218224119785347
9	0.7220874207846738	2.25659561947752
>10	5.849729106631198	34.062479999280896
>50	0.06770281958487652	1.6034726769890146
>100	0.03853852807138883	2.187211235838637
>500	0.002083163679534531	0.44756906231505805
>1k	0.0031247455193017964	2.496079097832221
>5k	0.0	0.0
>10k+	0.002083163679534531	11.011283948859072
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17596	3.126971461676118	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCG	14933	2.653731804797083	No Hit
GAATCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTC	14924	2.6521324218035	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGC	13438	2.3880565186408087	No Hit
GCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC	3744	0.6653433253304948	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCT	2531	0.449782039639819	No Hit
GAACTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCT	2480	0.4407188693428491	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTC	2221	0.3946921809719628	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACTAGCACTCGTA	1655	0.29410876159774807	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTT	1172	0.20827520760879803	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	714	0.12688438415757833	No Hit
CTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGCT	678	0.12048685218324663	TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC	573	0.10182738392477925	TruSeq Adapter, Index 16 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15140825672584976	0.0
2	0.0	0.0	0.0	1.2523168839754264	0.0
3	0.0	0.0	0.0	1.4790738506211827	0.0
4	0.0	0.0	0.0	2.1963082686323676	0.0
5	0.0	0.0	0.0	5.883419196505526	0.0
6	0.0	0.0	0.0	6.592301281105778	0.0
7	0.0	0.0	0.0	7.516744651396706	0.0
8	0.0	0.0	0.0	8.51173858262679	0.0
9	0.0	0.0	0.0	8.779901797884195	0.0
10	0.0	0.0	0.0	12.151578857578498	0.0
11	0.0	0.0	0.0	12.864192835830444	0.0
12	0.0	0.0	0.0	16.03026032623859	0.0
13	0.0	0.0	0.0	16.40700387583812	0.0
14	0.0	0.0	0.0	16.613324282010318	0.0
15	0.0	0.0	0.0	17.13063582582364	0.0
16	0.0	0.0	0.0	17.551806680800475	0.0
17	0.0	0.0	0.0	17.99057074870672	0.0
18	0.0	0.0	0.0	18.47411754043329	0.0
19	0.0	0.0	0.0	18.790084536276673	0.0
20	0.0	0.0	0.0	19.057714623869547	0.0
21	0.0	0.0	0.0	19.388076066655174	0.0
22	0.0	0.0	0.0	19.72981089961739	0.0
23	0.0	0.0	0.0	20.048976661447938	0.0
24	0.0	0.0	0.0	20.305588777307243	0.0
25	0.0	0.0	0.0	20.542830588022042	0.0
26	0.0	0.0	0.0	20.754659980061025	0.0
27	0.0	0.0	0.0	20.994567429098463	0.0
28	0.0	0.0	0.0	21.2229237787378	0.0
29	0.0	0.0	0.0	21.470295015078626	0.0
30	0.0	0.0	0.0	21.769024216435614	0.0
31	0.0	0.0	0.0	22.0137298144538	0.0
32	0.0	0.0	0.0	22.253992681934257	0.0
33	0.0	0.0	0.0	22.507761450249415	0.0
34	1.7770922150921333E-4	0.0	0.0	22.740027402761957	0.0
35	1.7770922150921333E-4	0.0	0.0	22.99504013562768	0.0
36	1.7770922150921333E-4	0.0	0.0	23.225706705146635	0.0
37	1.7770922150921333E-4	0.0	0.0	23.456728693108616	0.0
38	1.7770922150921333E-4	0.0	0.0	23.70161200034831	0.0
39	1.7770922150921333E-4	0.0	0.0	23.973329400035897	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCCA	25	3.888258E-5	45.000004	42
ATTACGG	20	7.030212E-4	45.000004	2
TAGCCGT	20	7.030212E-4	45.000004	44
GTATGCG	20	7.030212E-4	45.000004	1
TATCGGG	25	3.888258E-5	45.000004	3
CGATAGA	25	3.888258E-5	45.000004	10
CGTTTTT	7035	0.0	44.00853	1
TACGGCT	1610	0.0	42.624226	7
AACACGT	355	0.0	41.830986	41
TCAAGCG	395	0.0	41.58228	17
ACGGCTG	1675	0.0	40.97015	8
ACCGCGC	55	6.002665E-11	40.909092	35
GACACGA	355	0.0	40.56338	25
GCGTAAG	50	1.0786607E-9	40.500004	1
GAATCTG	2035	0.0	40.2457	1
CGAGACA	370	0.0	40.135136	22
GTCAAGC	415	0.0	40.120483	16
CACGACC	365	0.0	40.068493	27
ACACGAC	360	0.0	40.0	26
CGTAAGG	90	0.0	40.0	2
>>END_MODULE