##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552355_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	383577
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.1276640674493	30.0	28.0	31.0	16.0	33.0
2	28.589375796776135	31.0	28.0	33.0	16.0	33.0
3	28.231544122822797	31.0	27.0	33.0	16.0	34.0
4	32.19086650138043	35.0	32.0	35.0	25.0	37.0
5	33.326291722392114	35.0	32.0	35.0	28.0	37.0
6	33.27763656319331	35.0	33.0	35.0	28.0	37.0
7	34.09798554136458	35.0	33.0	36.0	31.0	37.0
8	34.14566566817093	35.0	35.0	37.0	30.0	37.0
9	35.42520015537949	37.0	34.0	39.0	30.0	39.0
10	35.44598607320042	37.0	34.0	39.0	30.0	39.0
11	35.765228363535876	37.0	35.0	39.0	31.0	39.0
12	35.72019959486622	37.0	35.0	39.0	31.0	39.0
13	35.792727405449234	37.0	35.0	39.0	30.0	39.0
14	36.89130213751085	38.0	36.0	40.0	31.0	41.0
15	37.00095678312307	39.0	36.0	40.0	31.0	41.0
16	36.738034345124966	38.0	35.0	40.0	31.0	41.0
17	35.80919085346619	38.0	34.0	40.0	29.0	41.0
18	35.538436871866665	38.0	33.0	39.0	29.0	40.0
19	34.90738756494785	37.0	33.0	39.0	27.0	40.0
20	35.41344501886192	37.0	34.0	39.0	29.0	40.0
21	35.81475427358783	37.0	34.0	39.0	30.0	40.0
22	36.21049750115361	38.0	35.0	40.0	31.0	41.0
23	36.42975204456993	38.0	35.0	40.0	31.0	41.0
24	36.32575467246472	38.0	35.0	40.0	31.0	41.0
25	35.58350213907507	37.0	34.0	40.0	29.0	41.0
26	35.68454312954113	38.0	34.0	40.0	30.0	41.0
27	36.016815398212096	38.0	35.0	40.0	30.0	41.0
28	35.824017081316136	38.0	35.0	40.0	30.0	41.0
29	35.923598130232	38.0	35.0	40.0	30.0	41.0
30	35.426039621770855	38.0	34.0	40.0	27.0	41.0
31	35.4925608156902	38.0	34.0	40.0	28.0	41.0
32	35.322667938901446	38.0	34.0	40.0	27.0	41.0
33	34.930465069594895	38.0	34.0	40.0	24.0	41.0
34	34.790962440396584	38.0	34.0	40.0	23.0	41.0
35	34.46286143329762	38.0	34.0	40.0	21.0	41.0
36	34.25117772963447	38.0	34.0	40.0	20.0	41.0
37	34.2164571911246	38.0	34.0	40.0	19.0	41.0
38	34.0887331617902	38.0	33.0	40.0	18.0	41.0
39	33.87457537860716	37.0	33.0	40.0	18.0	41.0
40	33.78378787049276	37.0	33.0	40.0	18.0	41.0
41	33.511081217069844	37.0	33.0	40.0	17.0	41.0
42	33.49943557616854	37.0	33.0	40.0	17.0	41.0
43	33.412141499620674	37.0	33.0	40.0	16.0	41.0
44	33.31923446922	37.0	33.0	40.0	15.0	41.0
45	33.32692523274336	37.0	33.0	40.0	17.0	41.0
46	33.067334589925885	36.0	32.0	40.0	15.0	40.0
47	33.087562601511564	36.0	32.0	40.0	17.0	40.0
48	33.01074360558636	36.0	32.0	40.0	17.0	40.0
49	32.97233932170073	36.0	32.0	40.0	16.0	40.0
50	32.8490733281714	36.0	32.0	39.0	15.0	40.0
51	30.241448262017794	34.0	27.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	9.0
9	21.0
10	21.0
11	27.0
12	20.0
13	27.0
14	35.0
15	76.0
16	155.0
17	254.0
18	551.0
19	784.0
20	1164.0
21	1774.0
22	2408.0
23	3418.0
24	5036.0
25	7286.0
26	9571.0
27	10600.0
28	10473.0
29	10511.0
30	11855.0
31	14399.0
32	18428.0
33	25219.0
34	33190.0
35	39458.0
36	49446.0
37	60746.0
38	54224.0
39	12389.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.0518722446862	17.494271032934716	19.67479801969357	23.77905870268551
2	35.797766810835896	26.198911822137404	21.7872813020593	16.216040064967398
3	28.07259037950659	24.697518360068514	32.50325228050691	14.726638979917983
4	25.872771307977278	20.905059479582977	36.72874025293487	16.49342895950487
5	25.315647184268087	24.309330330025	30.783910401301434	19.59111208440548
6	21.637637293164083	32.63438631617641	32.478485414923206	13.249490975736292
7	67.40524066875751	5.35094648532107	22.555575542850587	4.688237303070831
8	66.26752907499667	8.332616397750648	19.69356869676753	5.706285830485144
9	61.243765919228736	6.414878890027295	20.545288168998663	11.796067021745307
10	35.20336203682702	20.795824567166434	28.28271768119569	15.718095714810845
11	27.4380372128673	21.152728135420006	32.35230475237045	19.056929899342244
12	23.796525860518226	18.86583397857536	37.58437028288974	19.75326987801667
13	20.875078537034288	20.779660928574966	40.93467543674412	17.410585097646628
14	19.450853414047245	24.487651762227664	34.53987074303203	21.521624080693055
15	17.424663105452098	21.431941956895226	43.094085411794765	18.04930952585791
16	19.333275978486718	21.731490678533905	35.88405978460648	23.051173558372895
17	19.077004095657458	20.72569523198732	36.836150238413666	23.361150433941553
18	20.565362365313874	22.03390714250333	36.57727131710191	20.823459175080885
19	20.83571225594861	23.307966848898655	33.47906678450481	22.377254110647925
20	21.7356619401059	22.67158875532162	36.80356225738248	18.789187047190005
21	22.137667274106633	24.565341509006014	34.452013546171955	18.844977670715398
22	20.168831812126378	20.901148921859235	33.4284902379444	25.501529028069985
23	20.03065877255414	24.250932668017114	35.38090135748494	20.337507201943808
24	21.83551151398546	21.431420549198727	33.88732901086353	22.845738925952286
25	19.823399213195785	25.843311773125084	32.80384381753859	21.52944519614054
26	18.789447751038253	21.636333773922836	34.34382144914841	25.2303970258905
27	21.70281325522646	22.99668645408875	33.25616499425148	22.04433529643331
28	18.116831822554534	21.98619833827367	36.35071967297309	23.546250166198703
29	20.19907345852332	21.347734613910635	33.053598104161615	25.399593823404427
30	19.873454352059692	21.974205961254196	36.43440560826118	21.717934078424932
31	21.12457211980906	21.36676599483285	32.00582933804686	25.502832547311233
32	20.837797886734606	22.727900786543508	33.66260229367246	22.771699033049426
33	18.64971048837652	22.462764972873764	31.08215560369887	27.80536893505085
34	19.903174590760134	21.3128002982452	34.0082955964513	24.775729514543364
35	18.924231640583248	21.568550773377964	31.937785633653736	27.56943195238505
36	20.396947679344695	25.392294115653442	28.96706528285064	25.243692922151222
37	19.23811907387565	23.509230219747273	35.74067266806925	21.511978038307824
38	18.736524869843603	25.18712018708108	28.633625060939526	27.44272988213579
39	21.54951939245575	22.866855937660496	33.331508406395585	22.252116263488166
40	20.23557199727825	21.59331763896167	33.01631745386194	25.154792909898145
41	18.6348503690263	25.94003290082565	30.873071117402763	24.552045612745292
42	19.371078036482896	23.34837594537733	32.658631774063615	24.621914244076155
43	22.367347364414446	23.44379355383665	29.773943693182854	24.41491538856605
44	21.562033177171728	22.159566397359594	31.55663660751296	24.721763817955715
45	20.296055290072136	21.099544550377107	31.013069083912747	27.59133107563801
46	23.53191145454498	23.399734603482482	30.21349038133152	22.85486356064102
47	19.151043988560314	21.48199709575913	36.84058220383391	22.526376711846645
48	20.618285246508524	23.066294381571367	30.798509816803403	25.516910555116706
49	18.88121550562208	21.38892582193406	35.975045427645554	23.754813244798306
50	19.998070791522952	20.391733602379706	34.42281471516801	25.18738089092933
51	20.29527317852739	20.564319549920874	30.51721036454219	28.623196907009547
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	174.0
1	443.0
2	712.0
3	7220.5
4	13729.0
5	9611.0
6	5493.0
7	5010.0
8	4527.0
9	4233.0
10	3939.0
11	3805.0
12	3671.0
13	3525.5
14	3380.0
15	3061.5
16	2743.0
17	2552.0
18	2361.0
19	2143.5
20	1926.0
21	1849.5
22	1773.0
23	1788.5
24	1804.0
25	1943.5
26	2349.0
27	2615.0
28	2896.5
29	3178.0
30	3881.5
31	4585.0
32	4973.0
33	5361.0
34	5983.5
35	6606.0
36	7235.0
37	7864.0
38	8854.0
39	9844.0
40	10899.0
41	11954.0
42	13434.5
43	14915.0
44	17705.0
45	20495.0
46	33947.0
47	47399.0
48	39743.5
49	32088.0
50	31171.5
51	30255.0
52	26859.0
53	23463.0
54	21405.0
55	19347.0
56	18469.5
57	17592.0
58	16938.5
59	16285.0
60	15310.5
61	14336.0
62	13378.0
63	12420.0
64	11122.0
65	9824.0
66	8834.5
67	7845.0
68	6855.5
69	5866.0
70	4892.5
71	3919.0
72	3389.5
73	2860.0
74	2364.0
75	1531.0
76	1194.0
77	875.5
78	557.0
79	445.5
80	334.0
81	239.0
82	144.0
83	121.5
84	99.0
85	85.0
86	71.0
87	50.5
88	30.0
89	28.0
90	26.0
91	18.5
92	11.0
93	9.0
94	7.0
95	5.5
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	383577.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.496465695815864
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.41178677173946	29.8008019391008
2	7.479624144367272	5.758781885824615
3	2.7999315754766765	3.2336240953790862
4	1.7111786563016689	2.634973217669179
5	1.2741945405774127	2.4525993210567107
6	1.0687406503415897	2.4685642670159362
7	0.9749825358079245	2.6273367220624526
8	0.84005359021746	2.5871275374762726
9	0.7730819130142739	2.678482920996873
>10	5.572215701985119	33.910151673044034
>50	0.056388419372499984	1.462612263095287
>100	0.030257200638902432	1.8562601246740547
>500	0.002750654603536585	0.6856408617948947
>1k	0.002750654603536585	2.4550707923883603
>5k	0.0020629909526524385	5.387972378421424
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8188	2.134643109466939	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTC	6423	1.6745008173065643	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCG	5742	1.496961496648652	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGC	4880	1.2722347794575797	No Hit
GCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC	2081	0.5425247082072179	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCT	1213	0.31623376792664837	No Hit
GAACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCT	1100	0.28677423307445443	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTC	863	0.22498742103932196	No Hit
CCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC	662	0.17258594754117165	No Hit
CTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGCT	533	0.13895515111698564	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTA	532	0.13869444726873612	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.17310735523767065	0.0
2	0.0	0.0	0.0	1.2336506099166529	0.0
3	0.0	0.0	0.0	1.5391955200650718	0.0
4	0.0	0.0	0.0	2.2089437062180477	0.0
5	0.0	0.0	0.0	5.109534721842029	0.0
6	0.0	0.0	0.0	5.829859454555408	0.0
7	0.0	0.0	0.0	6.857032616658454	0.0
8	0.0	0.0	0.0	8.076865922617884	0.0
9	0.0	0.0	0.0	8.467660991143891	0.0
10	0.0	0.0	0.0	10.885167775961541	0.0
11	0.0	0.0	0.0	11.804670248737542	0.0
12	0.0	0.0	0.0	14.032384632029553	0.0
13	0.0	0.0	0.0	14.445600231505017	0.0
14	0.0	0.0	0.0	14.63382840994116	0.0
15	0.0	0.0	0.0	15.029316147735656	0.0
16	0.0	0.0	0.0	15.533256686401948	0.0
17	0.0	0.0	0.0	16.090641513959387	0.0
18	0.0	0.0	0.0	16.7669072963186	0.0
19	0.0	0.0	0.0	17.136585353136397	0.0
20	0.0	0.0	0.0	17.4515156018218	0.0
21	0.0	0.0	0.0	17.853260231974286	0.0
22	0.0	0.0	0.0	18.278468208469224	0.0
23	0.0	0.0	0.0	18.70211196187467	0.0
24	0.0	0.0	0.0	19.025906141400554	0.0
25	0.0	0.0	0.0	19.341097093934202	0.0
26	0.0	0.0	0.0	19.606493611452198	0.0
27	2.60703848249504E-4	0.0	0.0	19.901871071518887	0.0
28	2.60703848249504E-4	0.0	0.0	20.18942741613809	0.0
29	2.60703848249504E-4	0.0	0.0	20.47255179533705	0.0
30	2.60703848249504E-4	0.0	0.0	20.816159467329896	0.0
31	2.60703848249504E-4	0.0	0.0	21.121182969781817	0.0
32	2.60703848249504E-4	0.0	0.0	21.419167468331	0.0
33	2.60703848249504E-4	0.0	0.0	21.722887451541673	0.0
34	2.60703848249504E-4	0.0	0.0	21.995322972962406	0.0
35	2.60703848249504E-4	0.0	0.0	22.327459675632273	0.0
36	5.21407696499008E-4	0.0	0.0	22.6272691011192	0.0
37	5.21407696499008E-4	0.0	0.0	22.919778818855146	0.0
38	5.21407696499008E-4	0.0	0.0	23.193517859517126	0.0
39	5.21407696499008E-4	0.0	0.0	23.489156023432063	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCTAGA	25	3.8859296E-5	45.0	31
ATAGCGG	40	6.7939254E-9	45.0	2
TCGGGAT	25	3.8859296E-5	45.0	5
TACGGGT	20	7.027404E-4	45.0	4
TGATTCG	35	1.2091004E-7	45.0	15
TTCGCCA	20	7.027404E-4	45.0	12
CGTTTTT	3995	0.0	43.648315	1
TACGGCT	510	0.0	40.588234	7
TCGTTCA	50	1.0768417E-9	40.5	16
ACGGGTA	45	1.9224899E-8	40.0	5
TTACGGG	90	0.0	40.0	3
TTAACGG	35	6.2374093E-6	38.571426	2
GAATGAC	205	0.0	38.414635	1
ACGGGAT	65	9.094947E-12	38.076927	5
ATGGGAA	190	0.0	37.894737	5
TACGGGA	95	0.0	37.894737	4
ACGGCTG	550	0.0	37.636364	8
AATGACT	210	0.0	37.5	2
GTGTTAT	30	1.13850445E-4	37.499996	45
GCGAATG	30	1.13850445E-4	37.499996	1
>>END_MODULE