FastQCFastQC Report
Sat 18 Jun 2016
SRR3552352_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552352_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435700
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100702.3112233187973374No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGC51591.1840716089052101No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCG46031.0564608675694285No Hit
GAATCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTC40150.9215056231351848No Hit
GCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTCTGC22560.5177874684415882TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAAAGCTGT10880.24971310534771635No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTA9150.2100068854716548No Hit
GAACTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTCT8440.1937112692219417No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCT8250.18935047050722975No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTC6570.15079182924030296No Hit
CTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTCTGCT5220.11980720679366536TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTCTGC4420.10144594904750975TruSeq Adapter, Index 16 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAGG302.1623873E-645.0000042
TTAGCGG900.045.02
ATTGTCG207.02846E-445.01
ATATGCG207.02846E-445.01
TCGATGG351.2096098E-745.02
CTAACGG207.02846E-445.02
CGGATAA207.02846E-445.041
GCTAGTC207.02846E-445.027
CAGTAAT207.02846E-445.018
TATCGAG207.02846E-445.01
GTTATTC253.8868056E-545.09
GCATCGC351.2096098E-745.022
CGTTTTT51800.043.696911
TACGGCT5950.041.974797
CGTATGG603.6379788E-1241.2500042
CACAACG1200.041.25000412
ACGGCTG6000.040.8750048
CGCATGG451.9235813E-840.02
CGCATCG403.4516415E-739.37521
CCAGTCG403.4516415E-739.37528
Produced by FastQC (version 0.11.5)