##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552352_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	435700
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.687339912784026	28.0	16.0	31.0	16.0	33.0
2	27.004886389717697	30.0	25.0	31.0	16.0	33.0
3	27.207521230204268	31.0	25.0	33.0	16.0	34.0
4	30.601813174202434	33.0	28.0	35.0	19.0	37.0
5	32.40582281386275	33.0	32.0	35.0	28.0	37.0
6	31.333828322240073	35.0	30.0	35.0	17.0	37.0
7	33.26886619233417	35.0	32.0	35.0	28.0	37.0
8	33.713068625200826	35.0	35.0	37.0	30.0	37.0
9	35.218840945604775	37.0	34.0	39.0	30.0	39.0
10	35.28190039017673	37.0	34.0	39.0	30.0	39.0
11	35.60578838650448	37.0	35.0	39.0	30.0	39.0
12	35.35904291943998	37.0	34.0	39.0	30.0	39.0
13	35.50089281615791	37.0	34.0	39.0	30.0	39.0
14	35.88867798944228	38.0	34.0	40.0	27.0	41.0
15	36.27877438604544	38.0	34.0	40.0	30.0	41.0
16	35.972024328666514	38.0	34.0	40.0	29.0	41.0
17	35.882513197154005	37.0	34.0	40.0	30.0	41.0
18	35.54125086068396	38.0	34.0	39.0	27.0	40.0
19	34.88519164562773	37.0	33.0	39.0	27.0	40.0
20	35.34812715170989	36.0	34.0	39.0	30.0	40.0
21	35.26946063805371	37.0	34.0	39.0	29.0	40.0
22	35.89440440670186	37.0	34.0	39.0	30.0	40.0
23	36.412352536148724	38.0	35.0	40.0	31.0	41.0
24	36.395455588707826	38.0	35.0	40.0	31.0	41.0
25	34.495538214367684	36.0	33.0	39.0	25.0	41.0
26	35.15403718154693	37.0	33.0	39.0	28.0	41.0
27	35.793472572871245	37.0	35.0	40.0	30.0	41.0
28	35.8672985999541	38.0	35.0	40.0	30.0	41.0
29	35.970817075969705	38.0	35.0	40.0	30.0	41.0
30	35.1745099839339	37.0	34.0	40.0	27.0	41.0
31	35.191328896029376	37.0	34.0	40.0	28.0	41.0
32	35.01407390406243	37.0	34.0	40.0	27.0	41.0
33	35.00889373422079	38.0	34.0	40.0	25.0	41.0
34	34.85356437915997	38.0	34.0	40.0	23.0	41.0
35	34.662549919669495	38.0	34.0	40.0	22.0	41.0
36	34.43381455129676	38.0	34.0	40.0	21.0	41.0
37	34.4211934817535	38.0	34.0	40.0	21.0	41.0
38	34.1072481064953	38.0	33.0	40.0	20.0	41.0
39	34.14402111544641	38.0	34.0	40.0	20.0	41.0
40	33.873493229286204	37.0	33.0	40.0	18.0	41.0
41	33.73512967638283	37.0	33.0	40.0	18.0	41.0
42	33.97647463851274	37.0	33.0	40.0	18.0	41.0
43	34.02071149873766	37.0	33.0	40.0	18.0	41.0
44	34.07944227679596	38.0	34.0	40.0	18.0	41.0
45	34.09444571953179	37.0	34.0	40.0	20.0	41.0
46	33.94234794583429	37.0	33.0	40.0	20.0	41.0
47	33.881266926784484	37.0	33.0	40.0	20.0	41.0
48	33.900300665595594	37.0	33.0	40.0	20.0	41.0
49	33.95683497819601	37.0	34.0	40.0	20.0	41.0
50	33.853575854946065	37.0	33.0	40.0	20.0	41.0
51	32.86370897406472	35.0	32.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	14.0
9	27.0
10	32.0
11	27.0
12	29.0
13	47.0
14	47.0
15	77.0
16	134.0
17	249.0
18	407.0
19	762.0
20	1229.0
21	1919.0
22	2671.0
23	3848.0
24	5571.0
25	8227.0
26	10622.0
27	11530.0
28	11389.0
29	12057.0
30	13742.0
31	17624.0
32	23729.0
33	31489.0
34	38261.0
35	50369.0
36	63145.0
37	72268.0
38	47438.0
39	6720.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.725499196694976	17.02708285517558	20.18843240761992	22.058985540509525
2	40.11039706219876	21.97452375487721	21.490934128987835	16.424145053936197
3	29.2155152627955	21.476474638512737	33.61372504016525	15.694285058526509
4	25.314436538902914	21.762451227909114	35.7725499196695	17.150562313518474
5	25.518246499885244	24.78746844158825	31.984392930915767	17.70989212761074
6	22.38168464539821	30.761303649299975	32.661464310305256	14.195547394996558
7	67.71677759926555	5.263484048657333	22.98898324535231	4.03075510672481
8	67.95455588707826	7.697498278632087	19.21712187284829	5.130823961441359
9	62.73399127840257	5.981409226532017	20.860454441129217	10.424145053936194
10	37.45696580215745	20.854716548083545	27.600642644021118	14.087675005737893
11	29.06541198072068	21.025246729400966	32.66743171907276	17.2419095708056
12	24.9029148496672	19.494147349093414	36.48588478310764	19.11705301813174
13	22.378700941014458	20.841863667661233	39.51319715400505	17.266238237319257
14	19.986688088134034	24.180169841634154	36.01583658480606	19.817305485425752
15	19.04200137709433	21.176038558641267	41.55496901537755	18.22699104888685
16	21.732384668349784	21.27404177186137	36.591691530869866	20.40188202891898
17	21.257975671333487	21.09019967867799	37.19738352077117	20.45444112921735
18	21.714252926325454	20.451457424833603	37.38971769566215	20.44457195317879
19	21.390176727105807	23.05095249024558	35.0442965343126	20.51457424833601
20	23.016295616249714	22.975441817764516	36.45903144365389	17.54923112233188
21	22.60569198990131	24.734909341289878	35.1209547854028	17.538443883406014
22	20.345421161349552	21.17489098003213	36.60706908423227	21.872618774386048
23	20.487950424604083	23.98416341519394	35.803075510672485	19.72481064952949
24	21.722974523754875	21.446178563231584	35.80514115216892	21.02570576084462
25	20.26072985999541	23.644250631168234	34.81937112692219	21.275648381914163
26	18.920817075969705	21.8551755795272	36.28735368372734	22.936653660775765
27	20.379389488179942	23.341519394078496	35.178563231581364	21.100527886160204
28	17.227450080330502	24.023869635070003	37.751893504705066	20.99678677989442
29	20.06242827633693	22.60110167546477	35.09088822584347	22.245581822354833
30	21.107872389258663	23.966031673169613	35.30112462703695	19.62497131053477
31	21.783337158595366	24.03167316961212	32.88340601331191	21.301583658480606
32	22.826945145742485	25.602937801239385	32.47601560706909	19.094101445949047
33	23.49139316043149	23.186137250401654	30.587330732155156	22.735138857011705
34	21.19072756483819	24.061739729171446	31.963507000229512	22.784025705760843
35	20.204498508147807	25.621299058985542	32.00734450309846	22.166857929768188
36	22.910947899931145	27.060362634840484	29.312829928850125	20.715859536378243
37	20.636676612347944	28.214138168464544	31.206105118200593	19.94308010098692
38	22.41473490934129	27.981868257975673	28.719072756483822	20.88432407619922
39	23.062657792058754	25.473261418407162	28.702088593068627	22.761992196465457
40	23.231351847601562	23.2359421620381	32.085150332797795	21.447555657562543
41	20.91691530869865	24.499426210695432	30.018820289189808	24.56483819141611
42	20.99495065411981	24.719302272205645	32.3424374569658	21.943309616708746
43	23.490934128987835	24.00986917603856	29.50034427358274	22.998852421390865
44	21.323617167775993	24.544870323617168	30.938719302272204	23.192793206334635
45	21.13679137020886	23.581133807665825	30.500573789304568	24.78150103282075
46	23.59697039247188	24.82809272435162	30.24259811797108	21.332338765205417
47	19.91301354142759	24.31351847601561	34.470736745467065	21.30273123708974
48	21.21620380996098	24.138627495983474	31.571264631627265	23.073904062428277
49	21.368372733532247	22.52283681432178	33.98485196235942	22.12393848978655
50	21.29538673399128	22.58503557493688	32.837273353224695	23.282304337847144
51	20.312829928850125	22.31696121184301	31.108560936424144	26.26164792288272
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	147.0
1	513.0
2	879.0
3	8899.5
4	16920.0
5	11316.0
6	5712.0
7	5489.5
8	5267.0
9	5102.0
10	4937.0
11	4718.0
12	4499.0
13	4252.5
14	4006.0
15	3870.0
16	3734.0
17	3378.0
18	3022.0
19	2810.5
20	2599.0
21	2611.5
22	2624.0
23	2658.5
24	2693.0
25	2593.0
26	2724.5
27	2956.0
28	3415.0
29	3874.0
30	4751.5
31	5629.0
32	6459.5
33	7290.0
34	8170.5
35	9051.0
36	9602.0
37	10153.0
38	11380.5
39	12608.0
40	13743.5
41	14879.0
42	16334.0
43	17789.0
44	20059.0
45	22329.0
46	33540.5
47	44752.0
48	39430.0
49	34108.0
50	34250.5
51	34393.0
52	30918.5
53	27444.0
54	25611.5
55	23779.0
56	22117.0
57	20455.0
58	19332.0
59	18209.0
60	17314.5
61	16420.0
62	14764.0
63	13108.0
64	11997.5
65	10887.0
66	9468.0
67	8049.0
68	6789.5
69	5530.0
70	4768.0
71	4006.0
72	3558.0
73	3110.0
74	2577.0
75	1735.0
76	1426.0
77	1168.5
78	911.0
79	719.5
80	528.0
81	359.5
82	191.0
83	156.0
84	121.0
85	99.5
86	78.0
87	52.5
88	27.0
89	28.5
90	30.0
91	16.5
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	435700.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.85634012096178
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.13635267737462	29.201000026451553
2	7.787704387700687	5.896279721246073
3	2.7964569294898385	3.175908739691633
4	1.690521025181989	2.5598775564370557
5	1.186338326583643	2.245521359484151
6	0.9467193100202732	2.1503596919525836
7	0.8457502980052206	2.2411907657083296
8	0.7820770159032242	2.3685258811855436
9	0.6997102050996238	2.383962075932336
>10	6.048417132765231	37.89502994792758
>50	0.05065704905098914	1.2647515275606422
>100	0.02258205801068191	1.4657624572240984
>500	0.0030516294609029605	0.8694300325558452
>1k	0.0024413035687223684	2.7637847593497264
>5k	6.103258921805921E-4	1.1919717081997359
>10k+	6.103258921805921E-4	2.326643749093107
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10070	2.3112233187973374	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGC	5159	1.1840716089052101	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCG	4603	1.0564608675694285	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTC	4015	0.9215056231351848	No Hit
GCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTCTGC	2256	0.5177874684415882	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAAAGCTGT	1088	0.24971310534771635	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTA	915	0.2100068854716548	No Hit
GAACTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTCT	844	0.1937112692219417	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCT	825	0.18935047050722975	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTC	657	0.15079182924030296	No Hit
CTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTCTGCT	522	0.11980720679366536	TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTCTGC	442	0.10144594904750975	TruSeq Adapter, Index 16 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12531558411751206	0.0
2	0.0	0.0	0.0	0.9244893275189351	0.0
3	0.0	0.0	0.0	1.1333486343814552	0.0
4	0.0	0.0	0.0	1.6261188891439065	0.0
5	0.0	0.0	0.0	3.301583658480606	0.0
6	0.0	0.0	0.0	3.778058296993344	0.0
7	0.0	0.0	0.0	4.403259123249943	0.0
8	0.0	0.0	0.0	5.276107413357815	0.0
9	0.0	0.0	0.0	5.598117971081019	0.0
10	0.0	0.0	0.0	7.277943539132431	0.0
11	0.0	0.0	0.0	8.122331879733762	0.0
12	0.0	0.0	0.0	10.008721597429425	0.0
13	0.0	0.0	0.0	10.38168464539821	0.0
14	0.0	0.0	0.0	10.545329355060822	0.0
15	0.0	0.0	0.0	10.973146660546247	0.0
16	0.0	0.0	0.0	11.455818223548313	0.0
17	0.0	0.0	0.0	12.009869176038558	0.0
18	0.0	0.0	0.0	12.612347945834289	0.0
19	0.0	0.0	0.0	13.227220564608675	0.0
20	0.0	0.0	0.0	13.599954096855635	0.0
21	0.0	0.0	0.0	14.019049804911637	0.0
22	0.0	0.0	0.0	14.461785632315813	0.0
23	0.0	0.0	0.0	14.863667661234794	0.0
24	0.0	0.0	0.0	15.22469589166858	0.0
25	0.0	0.0	0.0	15.54395226072986	0.0
26	0.0	0.0	0.0	15.831535460179023	0.0
27	0.0	0.0	0.0	16.136332338765204	0.0
28	0.0	0.0	0.0	16.426210695432637	0.0
29	0.0	0.0	0.0	16.757172366307092	0.0
30	0.0	0.0	0.0	17.093642414505393	0.0
31	0.0	0.0	0.0	17.419095708056002	0.0
32	0.0	0.0	0.0	17.725958228138627	0.0
33	0.0	0.0	0.0	18.053018131742025	0.0
34	0.0	0.0	0.0	18.394537525820517	0.0
35	0.0	0.0	0.0	18.71907275648382	0.0
36	0.0	0.0	0.0	19.026394308010097	0.0
37	0.0	0.0	0.0	19.34128987835667	0.0
38	0.0	0.0	0.0	19.69130135414276	0.0
39	0.0	0.0	0.0	20.06242827633693	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTAGG	30	2.1623873E-6	45.000004	2
TTAGCGG	90	0.0	45.0	2
ATTGTCG	20	7.02846E-4	45.0	1
ATATGCG	20	7.02846E-4	45.0	1
TCGATGG	35	1.2096098E-7	45.0	2
CTAACGG	20	7.02846E-4	45.0	2
CGGATAA	20	7.02846E-4	45.0	41
GCTAGTC	20	7.02846E-4	45.0	27
CAGTAAT	20	7.02846E-4	45.0	18
TATCGAG	20	7.02846E-4	45.0	1
GTTATTC	25	3.8868056E-5	45.0	9
GCATCGC	35	1.2096098E-7	45.0	22
CGTTTTT	5180	0.0	43.69691	1
TACGGCT	595	0.0	41.97479	7
CGTATGG	60	3.6379788E-12	41.250004	2
CACAACG	120	0.0	41.250004	12
ACGGCTG	600	0.0	40.875004	8
CGCATGG	45	1.9235813E-8	40.0	2
CGCATCG	40	3.4516415E-7	39.375	21
CCAGTCG	40	3.4516415E-7	39.375	28
>>END_MODULE