FastQCFastQC Report
Sat 18 Jun 2016
SRR3552351_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552351_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences431328
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76081.7638548853772535No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCG10400.24111580977817346No Hit
GAATCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTC9540.2211773870465168No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGC9310.21584501817642257No Hit
GCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC9000.2086579123080347Illumina PCR Primer Index 7 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC5930.1374823799985162Illumina PCR Primer Index 7 (95% over 23bp)
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT5200.12055790488908673No Hit
CTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGCT4970.1152255360189925Illumina PCR Primer Index 7 (95% over 24bp)
AAGCAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG4750.11012500927368499No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAGG700.045.0000042
AACACGC351.2095734E-745.00000423
TCGATAA207.0283795E-445.025
GCTTGCG253.8867394E-545.01
CGCTCTA207.0283795E-445.036
TATCTGG207.0283795E-445.038
ACTACGG207.0283795E-445.02
GTCGTTT207.0283795E-445.09
CATACGA253.8867394E-545.018
TAATGCG207.0283795E-445.01
ATCGATA207.0283795E-445.024
AGTCGGC406.7975634E-945.030
TAGTACG207.0283795E-445.01
ACGGTCT207.0283795E-445.030
CGTTTTT40200.042.6492541
ACGGGAC1500.042.0000045
TATGCGG556.002665E-1140.9090922
TACGGGA1000.040.54
CGACAGG950.040.2631572
ATAACGC451.9235813E-840.011