##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552351_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	431328
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.99104162029824	30.0	26.0	31.0	16.0	33.0
2	28.613396301654426	31.0	28.0	33.0	16.0	34.0
3	28.300993675346835	31.0	27.0	33.0	16.0	34.0
4	32.296584038133396	35.0	32.0	35.0	25.0	37.0
5	33.40544087098449	35.0	33.0	35.0	28.0	37.0
6	33.23980358335188	35.0	33.0	35.0	28.0	37.0
7	34.238491356925586	35.0	35.0	36.0	31.0	37.0
8	34.45083324059648	35.0	35.0	37.0	32.0	37.0
9	35.79111256398843	37.0	35.0	39.0	30.0	39.0
10	35.648476333555905	37.0	35.0	39.0	31.0	39.0
11	35.89524677275762	37.0	35.0	39.0	31.0	39.0
12	35.78476472661177	37.0	35.0	39.0	31.0	39.0
13	35.99113899399065	37.0	35.0	39.0	31.0	39.0
14	36.93960976333556	39.0	36.0	40.0	31.0	41.0
15	37.28846956376586	39.0	36.0	40.0	32.0	41.0
16	36.913112526893684	38.0	35.0	40.0	32.0	41.0
17	36.021776930781215	38.0	34.0	40.0	30.0	41.0
18	35.795828232806585	38.0	34.0	39.0	29.0	41.0
19	35.191056458194225	37.0	33.0	39.0	27.0	41.0
20	35.80526652570666	37.0	34.0	39.0	30.0	40.0
21	36.03196871058684	38.0	35.0	39.0	30.0	40.0
22	36.43796136582833	38.0	35.0	40.0	31.0	41.0
23	36.667471158839675	38.0	35.0	40.0	31.0	41.0
24	36.589168799614214	38.0	35.0	40.0	31.0	41.0
25	35.81859512946064	38.0	34.0	40.0	30.0	41.0
26	36.02806217078418	38.0	35.0	40.0	30.0	41.0
27	36.31226120261147	38.0	35.0	40.0	31.0	41.0
28	36.27515950738185	38.0	35.0	40.0	31.0	41.0
29	36.275984865346096	38.0	35.0	40.0	31.0	41.0
30	35.722014337116995	38.0	35.0	40.0	29.0	41.0
31	35.81464917649677	38.0	35.0	40.0	30.0	41.0
32	35.60496420357593	38.0	35.0	40.0	29.0	41.0
33	35.45245613546999	38.0	35.0	40.0	27.0	41.0
34	35.20554427257215	38.0	34.0	40.0	25.0	41.0
35	34.94802331404407	38.0	34.0	40.0	23.0	41.0
36	34.65076693374879	38.0	34.0	40.0	22.0	41.0
37	34.56613992135915	38.0	34.0	40.0	22.0	41.0
38	34.42156780918466	38.0	34.0	40.0	21.0	41.0
39	34.22269131612138	38.0	34.0	40.0	21.0	41.0
40	34.18036389939906	38.0	33.0	40.0	21.0	41.0
41	34.098458713554415	38.0	33.0	40.0	20.0	41.0
42	34.02820591290155	37.0	33.0	40.0	20.0	41.0
43	33.89320192521701	37.0	33.0	40.0	19.0	41.0
44	33.767654777802505	37.0	33.0	40.0	18.0	41.0
45	33.684961328733586	37.0	33.0	40.0	20.0	41.0
46	33.467820313079606	36.0	33.0	40.0	19.0	41.0
47	33.47623618220936	36.0	33.0	40.0	20.0	41.0
48	33.376453650122414	36.0	33.0	39.0	20.0	40.0
49	33.29290934045552	36.0	33.0	39.0	19.0	40.0
50	33.11178268046591	36.0	32.0	39.0	19.0	40.0
51	30.71115021514949	34.0	28.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	26.0
10	32.0
11	21.0
12	18.0
13	35.0
14	44.0
15	82.0
16	162.0
17	304.0
18	480.0
19	795.0
20	1165.0
21	1748.0
22	2436.0
23	3457.0
24	5106.0
25	7201.0
26	9601.0
27	10760.0
28	10537.0
29	10565.0
30	12255.0
31	15083.0
32	19418.0
33	27454.0
34	37093.0
35	43405.0
36	56056.0
37	71804.0
38	67826.0
39	16354.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.302869278136356	19.34768899770013	22.9614121967505	19.38802952741301
2	38.17697900437718	21.788059203204984	23.627494621262706	16.40746717115513
3	31.56460976333556	20.143834854217673	32.31809666889235	15.973458713554416
4	27.538207582164848	23.075710364270346	31.54559870910305	17.840483344461756
5	23.973171229319682	27.356211514207285	30.148286223013578	18.522331033459455
6	23.694960679575637	32.10016507159285	30.448753616737147	13.756120632094367
7	73.55585540470362	5.656020476296461	16.829883522516507	3.9582405964834186
8	74.9112044662067	5.250528600044514	15.771060538615625	4.06720639513317
9	68.76136026411454	7.009051116551674	17.77185807552489	6.4577305438088874
10	37.88879924326731	23.150827212701238	24.066603605608723	14.893769938422732
11	28.97841086133986	22.911102455671788	29.818143037317306	18.292343645671043
12	25.238101862155947	21.505211810965204	34.28574078195712	18.97094554492173
13	22.533199792269457	22.70128533273982	36.250834631649234	18.514680243341495
14	20.301487499072632	24.697909711402925	35.330885080495584	19.669717709028863
15	19.33540136508643	22.895800875435864	38.2917408561466	19.477056903331107
16	21.89632947548038	22.246179241783516	35.491319830847985	20.36617145188812
17	21.9378292158172	21.726389198011724	34.05134839379776	22.28443319237332
18	22.89811929668373	22.08319422805846	34.85305846131019	20.16562801394762
19	23.417909340455523	23.12323799985162	32.812384078937605	20.646468580755247
20	24.659655760813116	23.397507233474293	32.96887751316863	18.97395949254396
21	23.743647525780844	23.99797833667186	33.10682357741672	19.151550560130573
22	22.613417167445657	21.77438051784257	32.33363009125306	23.278572223458713
23	21.697408932413385	23.73066436679279	33.16246568736553	21.409461013428295
24	21.884505527116254	22.9648898286223	34.30475183618963	20.845852808071815
25	21.53952444543364	23.679659099339716	32.82513539580088	21.955681059425775
26	21.279629423547743	23.687541731582463	33.01617330662512	22.016655538244677
27	22.639615327546554	22.95863009125306	33.29183359299651	21.10992098820387
28	20.614010683285112	24.147748349284072	34.33628236516062	20.9019586022702
29	22.53366347651903	23.414663550708507	32.88819645374286	21.1634765190296
30	23.245418799614214	23.327954596038282	32.86338934639068	20.56323725795682
31	24.902626307589586	23.702379627568813	30.726732324356405	20.6682617404852
32	23.389856443356333	24.238862304325245	31.921646635507084	20.449634616811334
33	22.315036352845166	24.448679427257215	30.107018324801544	23.129265895096076
34	21.94246605831293	25.165535277097707	31.224961050523035	21.667037614066327
35	22.806773499517767	23.8403256918169	31.397219749239557	21.955681059425775
36	24.371707841828027	26.79863120409526	28.578946880332367	20.250714073744344
37	22.977409303360783	27.052730172861487	29.81860672156688	20.151253802210846
38	21.86085763038801	27.034182802878554	29.285601676682248	21.81935789005119
39	22.249888715780102	25.525354254766675	29.85546961940797	22.369287410045256
40	22.542473477260923	24.80664366792789	31.189257363305884	21.461625491505306
41	20.99747755768232	26.36439090436976	29.44348616366199	23.194645374285926
42	22.51163847466429	25.327129238074043	29.916675940351656	22.24455634691001
43	22.484512946064246	24.317920468877514	30.3676088730618	22.82995771199644
44	22.644252170042286	23.857713851175905	29.92131278284739	23.576721195934418
45	22.667668224645745	23.754775947770607	29.822779879813044	23.754775947770607
46	23.151986423325173	24.76421655909192	29.774788559982195	22.30900845760071
47	21.756296832109207	24.9093497292084	31.76051635878033	21.57383707990207
48	21.29655389865717	25.12542658950961	30.837552860004454	22.74046665182877
49	21.8279360486683	24.75540655835003	31.73084056680763	21.685816826174047
50	21.22004599747756	23.9536964908376	31.872959789301873	22.953297722382963
51	21.429863120409525	23.53846724534461	30.382910453297722	24.64875918094814
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	178.0
1	480.0
2	782.0
3	6963.0
4	13144.0
5	9271.5
6	5399.0
7	4939.0
8	4479.0
9	4251.0
10	4023.0
11	3854.5
12	3686.0
13	3541.5
14	3397.0
15	3082.0
16	2767.0
17	2612.0
18	2457.0
19	2262.0
20	2067.0
21	2227.0
22	2387.0
23	2166.0
24	1945.0
25	2313.5
26	2932.5
27	3183.0
28	3922.0
29	4661.0
30	5289.0
31	5917.0
32	6131.0
33	6345.0
34	7874.0
35	9403.0
36	10438.0
37	11473.0
38	12098.0
39	12723.0
40	13949.5
41	15176.0
42	17188.5
43	19201.0
44	21513.0
45	23825.0
46	28027.0
47	32229.0
48	34051.5
49	35874.0
50	34711.5
51	33549.0
52	30844.5
53	28140.0
54	26046.0
55	23952.0
56	23096.5
57	22241.0
58	20790.5
59	19340.0
60	18497.5
61	17655.0
62	16500.5
63	15346.0
64	14080.0
65	12814.0
66	10897.0
67	8980.0
68	7899.5
69	6819.0
70	5848.0
71	4877.0
72	4034.5
73	3192.0
74	2646.0
75	1728.0
76	1356.0
77	1034.5
78	713.0
79	514.0
80	315.0
81	270.0
82	225.0
83	186.5
84	148.0
85	106.5
86	65.0
87	52.5
88	40.0
89	32.5
90	25.0
91	20.0
92	15.0
93	14.5
94	14.0
95	7.5
96	1.0
97	2.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	431328.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.514073963481344
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.99298477887943	28.11327540885197
2	7.427728763412376	5.424332748958347
3	2.6892824814849074	2.9458997831290366
4	1.5047921758620415	2.197843712363784
5	1.1648594609285798	2.126688225670358
6	0.9618487247549681	2.1072609286429866
7	0.8078392791156362	2.0648252231763657
8	0.7767066614658948	2.2688579587755475
9	0.6676387009461683	2.1940388016507892
>10	6.9056519598759785	43.81454441282441
>50	0.06453013671411743	1.5909455264126422
>100	0.031619766989917544	2.1953257508273976
>500	0.0032265068357058718	0.9184694404556397
>1k	6.453013671411744E-4	0.24505085122469605
>5k	6.453013671411744E-4	1.792641227036046
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7608	1.7638548853772535	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCG	1040	0.24111580977817346	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTC	954	0.2211773870465168	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGC	931	0.21584501817642257	No Hit
GCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	900	0.2086579123080347	Illumina PCR Primer Index 7 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	593	0.1374823799985162	Illumina PCR Primer Index 7 (95% over 23bp)
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT	520	0.12055790488908673	No Hit
CTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGCT	497	0.1152255360189925	Illumina PCR Primer Index 7 (95% over 24bp)
AAGCAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	475	0.11012500927368499	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1370186957489428	0.0
2	0.0	0.0	0.0	0.6837024259959937	0.0
3	0.0	0.0	0.0	0.8851732324356406	0.0
4	0.0	0.0	0.0	1.175903256918169	0.0
5	0.0	0.0	0.0	2.129469916165888	0.0
6	0.0	0.0	0.0	2.5824894279991097	0.0
7	0.0	0.0	0.0	3.085123154536687	0.0
8	0.0	0.0	0.0	3.847188218710587	0.0
9	0.0	0.0	0.0	4.146264559685436	0.0
10	0.0	0.0	0.0	5.014281474886861	0.0
11	0.0	0.0	0.0	5.892035759329327	0.0
12	0.0	0.0	0.0	6.788801098004303	0.0
13	0.0	0.0	0.0	7.073039542992803	0.0
14	0.0	0.0	0.0	7.188265079011797	0.0
15	0.0	0.0	0.0	7.443291416277172	0.0
16	0.0	0.0	0.0	7.896542770235181	0.0
17	0.0	0.0	0.0	8.425606498998443	0.0
18	0.0	0.0	0.0	9.039988129683211	0.0
19	0.0	0.0	0.0	9.375231842124787	0.0
20	0.0	0.0	0.0	9.686595815713332	0.0
21	0.0	0.0	0.0	10.0865234809704	0.0
22	0.0	0.0	0.0	10.466280881371022	0.0
23	0.0	0.0	0.0	10.919764077453816	0.0
24	0.0	0.0	0.0	11.239242525409896	0.0
25	0.0	0.0	0.0	11.539941761258254	0.0
26	0.0	0.0	0.0	11.817920468877514	0.0
27	0.0	0.0	0.0	12.10517286148824	0.0
28	0.0	0.0	0.0	12.386629200979302	0.0
29	0.0	0.0	0.0	12.68501001557979	0.0
30	0.0	0.0	0.0	13.05943504711032	0.0
31	0.0	0.0	0.0	13.378681652941612	0.0
32	0.0	0.0	0.0	13.697232732398547	0.0
33	0.0	0.0	0.0	14.016711180354626	0.0
34	0.0	0.0	0.0	14.338508049558573	0.0
35	0.0	0.0	0.0	14.659377550263372	0.0
36	0.0	0.0	0.0	14.963090733733956	0.0
37	0.0	0.0	0.0	15.251270494843832	0.0
38	0.0	0.0	0.0	15.575153943170859	0.0
39	0.0	0.0	0.0	15.914570813858594	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAAGG	70	0.0	45.000004	2
AACACGC	35	1.2095734E-7	45.000004	23
TCGATAA	20	7.0283795E-4	45.0	25
GCTTGCG	25	3.8867394E-5	45.0	1
CGCTCTA	20	7.0283795E-4	45.0	36
TATCTGG	20	7.0283795E-4	45.0	38
ACTACGG	20	7.0283795E-4	45.0	2
GTCGTTT	20	7.0283795E-4	45.0	9
CATACGA	25	3.8867394E-5	45.0	18
TAATGCG	20	7.0283795E-4	45.0	1
ATCGATA	20	7.0283795E-4	45.0	24
AGTCGGC	40	6.7975634E-9	45.0	30
TAGTACG	20	7.0283795E-4	45.0	1
ACGGTCT	20	7.0283795E-4	45.0	30
CGTTTTT	4020	0.0	42.649254	1
ACGGGAC	150	0.0	42.000004	5
TATGCGG	55	6.002665E-11	40.909092	2
TACGGGA	100	0.0	40.5	4
CGACAGG	95	0.0	40.263157	2
ATAACGC	45	1.9235813E-8	40.0	11
>>END_MODULE