##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552350_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	341593
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.501187085215445	28.0	16.0	31.0	16.0	33.0
2	26.802071471019605	30.0	25.0	33.0	16.0	33.0
3	27.09314593683126	31.0	25.0	33.0	16.0	34.0
4	30.515707289083792	33.0	28.0	35.0	19.0	37.0
5	32.27819363979941	33.0	32.0	35.0	28.0	37.0
6	31.04125084530421	35.0	28.0	35.0	17.0	37.0
7	33.19366321909407	35.0	32.0	35.0	28.0	37.0
8	33.906008027096576	35.0	35.0	37.0	31.0	37.0
9	35.314757620911436	37.0	34.0	39.0	30.0	39.0
10	35.334070077548425	37.0	34.0	39.0	30.0	39.0
11	35.63683974788711	37.0	35.0	39.0	31.0	39.0
12	35.32442702280199	37.0	34.0	39.0	30.0	39.0
13	35.61943014054738	37.0	35.0	39.0	31.0	39.0
14	35.933286103637954	38.0	34.0	40.0	27.0	41.0
15	36.29075830008226	38.0	34.0	40.0	30.0	41.0
16	35.800628233014145	38.0	34.0	40.0	27.0	41.0
17	35.968816691208545	38.0	34.0	40.0	30.0	41.0
18	35.54665932849912	38.0	34.0	39.0	27.0	40.0
19	34.835921696287684	37.0	33.0	39.0	27.0	41.0
20	35.65585067609699	37.0	34.0	39.0	30.0	40.0
21	35.53640736197756	37.0	34.0	39.0	30.0	40.0
22	36.19845254440226	38.0	35.0	40.0	31.0	41.0
23	36.72031628282781	38.0	35.0	40.0	32.0	41.0
24	36.70891089688606	38.0	35.0	40.0	32.0	41.0
25	34.69157154859731	37.0	33.0	40.0	25.0	41.0
26	35.44974282259882	37.0	34.0	40.0	29.0	41.0
27	36.10856194359955	38.0	35.0	40.0	31.0	41.0
28	36.26007265956855	38.0	35.0	40.0	31.0	41.0
29	36.31969624670295	38.0	35.0	40.0	31.0	41.0
30	35.456798002300985	38.0	34.0	40.0	29.0	41.0
31	35.48466157093383	37.0	34.0	40.0	29.0	41.0
32	35.34507440140752	38.0	34.0	40.0	29.0	41.0
33	35.46333209404174	38.0	35.0	40.0	28.0	41.0
34	35.357410719774705	38.0	35.0	40.0	26.0	41.0
35	35.22282365270951	38.0	35.0	40.0	25.0	41.0
36	34.99332246269684	38.0	35.0	40.0	23.0	41.0
37	35.01067352082742	38.0	35.0	40.0	23.0	41.0
38	34.603806284086616	38.0	34.0	40.0	23.0	41.0
39	34.58954955165943	38.0	34.0	40.0	23.0	41.0
40	34.39735591771494	38.0	34.0	40.0	22.0	41.0
41	34.238201602491856	37.0	33.0	40.0	22.0	41.0
42	34.42882319017076	38.0	34.0	40.0	22.0	41.0
43	34.44984528371483	38.0	34.0	40.0	22.0	41.0
44	34.46054807914682	38.0	34.0	40.0	22.0	41.0
45	34.43464883648084	37.0	34.0	40.0	23.0	41.0
46	34.2814460483675	37.0	34.0	40.0	22.0	41.0
47	34.30688860720214	37.0	34.0	40.0	23.0	41.0
48	34.24636336224688	37.0	34.0	40.0	23.0	41.0
49	34.2224196631664	37.0	34.0	40.0	23.0	41.0
50	34.14027219527332	36.0	34.0	40.0	23.0	41.0
51	32.99357129683571	35.0	32.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	14.0
9	28.0
10	39.0
11	34.0
12	32.0
13	33.0
14	49.0
15	69.0
16	112.0
17	176.0
18	309.0
19	490.0
20	801.0
21	1215.0
22	1709.0
23	2497.0
24	3686.0
25	5741.0
26	7387.0
27	8171.0
28	8276.0
29	8606.0
30	10277.0
31	13212.0
32	18142.0
33	24419.0
34	29951.0
35	40644.0
36	53129.0
37	59522.0
38	37521.0
39	5301.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.15505587058283	18.46554232668702	22.800233025852403	20.579168776877747
2	39.52247265020068	19.555728600995923	23.220616347524686	17.701182401278714
3	31.534896792381577	18.44651383371439	32.9673031941521	17.051286179751926
4	27.447283755814667	23.4270023097663	30.963456511111175	18.162257423307853
5	23.956872652542646	26.96220355803544	30.5574177456798	18.523506043742113
6	24.09592702426572	30.271990351090334	30.699692323905936	14.932390300738014
7	72.76027319060988	5.569200774020545	17.59052439599172	4.080001639377856
8	74.09841536565445	4.9195972985394905	16.349573908130434	4.632413427675626
9	67.94313700807686	6.79229375309213	18.475202946196205	6.789366292634802
10	37.7030559759714	22.672595749912908	24.674393210633706	14.94995506348198
11	28.714288641746172	22.727046514419204	30.187386743873557	18.371278099961064
12	25.26310550860234	21.12572564426086	34.481971234773546	19.129197612363253
13	22.640100938836568	22.204202076740447	36.552856762287284	18.6028402221357
14	20.33794603519393	23.785323469743233	35.906180747263555	19.970549747799282
15	19.351391861074436	22.498997344793363	38.40915943827889	19.74045135585331
16	21.75044570585463	21.92814255561443	35.99400456098339	20.32740717754755
17	21.80313999408653	21.45330846943585	34.27763449485206	22.46591704162556
18	22.809893645361583	21.649741066122548	35.302831147008284	20.237534141507584
19	23.29468109709508	22.930797762249227	33.36631605448589	20.408205086169797
20	24.491426932050718	22.816041312321975	33.74161648511533	18.950915270511985
21	23.533269124367305	23.112885802695022	33.80631336122227	19.547531711715404
22	22.46796626394569	21.45447945361878	33.14324356763751	22.93431071479802
23	21.569528649591767	23.20978474383257	33.60548957384958	21.61519703272608
24	21.639202208476167	22.883665648886247	34.66083906871628	20.816293073921305
25	21.68252862324462	22.32305697130796	33.476681313727155	22.517733091720263
26	21.005407019464684	23.689888258834344	33.56187041303539	21.742834308665575
27	22.46386781930543	22.69250248102274	33.8484687918078	20.995160907864037
28	20.570386395505764	23.95218871581092	34.56833131826472	20.909093570418598
29	22.27241190539619	23.022134528517856	33.55367352375488	21.15178004233108
30	23.070144880018034	23.035893592667296	33.16373579083881	20.730225736475866
31	24.73996832487785	23.44690904087613	31.02698240303519	20.786140231210826
32	23.383090402906383	24.29645806559268	31.868920030562688	20.45153150093825
33	22.40502586411314	24.530362156133176	29.99212513136979	23.072486848383893
34	22.11813473929501	25.1940174418094	30.86743580811082	21.820412010784764
35	22.715336672589896	23.986440003161658	31.314751765990522	21.98347155825793
36	24.79968851820734	26.432040469213362	28.267850922003674	20.500420090575627
37	23.17582620252757	27.110333057176227	29.681814322893036	20.032026417403166
38	21.955075191821845	26.937612890193886	29.400192626898093	21.707119291086173
39	22.318080288530503	25.576343777536426	29.8235034090277	22.28207252490537
40	22.705383307034978	24.945768795028002	31.20819220534379	21.14065569259323
41	21.196570187328195	26.110898057044494	29.613311748191563	23.07922000743575
42	22.452743469567586	25.56024274502112	29.61448273237449	22.372531053036802
43	22.61375379472062	24.335100543629405	30.44295404179828	22.608191619851695
44	23.041163021490487	23.806401185035995	29.865073347521758	23.28736244595176
45	22.782668263108434	23.621092938087138	29.924793540851248	23.671445257953177
46	22.97939360584087	24.521579774761193	30.0038349731991	22.49519164619884
47	21.675209972101303	25.018369814369734	31.766751660601944	21.539668552927022
48	21.32128000281036	24.93200973087856	31.160767345935074	22.585942920376002
49	21.934290222574816	24.507235218520286	31.845500346904064	21.712974212000834
50	21.134508025632844	24.059919260640587	31.64467655953137	23.1608961541952
51	21.23199245886186	23.835675789609272	30.44090481947815	24.491426932050718
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	114.0
1	329.5
2	545.0
3	5486.0
4	10427.0
5	7214.0
6	4001.0
7	3848.5
8	3696.0
9	3613.5
10	3531.0
11	3389.5
12	3248.0
13	3055.5
14	2863.0
15	2604.5
16	2346.0
17	2190.0
18	2034.0
19	1967.5
20	1901.0
21	1907.5
22	1914.0
23	1765.0
24	1616.0
25	1822.0
26	2260.5
27	2493.0
28	3001.0
29	3509.0
30	3999.5
31	4490.0
32	4677.5
33	4865.0
34	5939.0
35	7013.0
36	7917.0
37	8821.0
38	9258.0
39	9695.0
40	10709.5
41	11724.0
42	13315.0
43	14906.0
44	16563.0
45	18220.0
46	21606.5
47	24993.0
48	26415.5
49	27838.0
50	27351.5
51	26865.0
52	24890.0
53	22915.0
54	20910.5
55	18906.0
56	18284.5
57	17663.0
58	16611.0
59	15559.0
60	14755.5
61	13952.0
62	13048.0
63	12144.0
64	11352.0
65	10560.0
66	9024.0
67	7488.0
68	6547.5
69	5607.0
70	4772.0
71	3937.0
72	3346.0
73	2755.0
74	2315.5
75	1571.5
76	1267.0
77	956.5
78	646.0
79	487.5
80	329.0
81	241.0
82	153.0
83	108.0
84	63.0
85	52.5
86	42.0
87	32.5
88	23.0
89	14.5
90	6.0
91	4.5
92	3.0
93	2.0
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	341593.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.47159891339463
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.75314940173	29.90097929611547
2	7.5986680721391	5.998631568389843
3	3.005729257869266	3.559228191266129
4	1.8986294542336628	2.997677612106739
5	1.4427163498672955	2.8473160553879304
6	1.208880644283317	2.8629871155190307
7	1.0626997724770118	2.936252142919784
8	0.9790389679349358	3.091538677032929
9	0.9305671435161259	3.305787574576667
>10	6.03877876695603	36.42219415160305
>50	0.050620802674707435	1.4104122493059854
>100	0.026054824906099415	1.8579243025753633
>500	0.0037221178437284873	1.024605731943744
>1k	0.0	0.0
>5k	7.444235687456975E-4	1.7844653312573435
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6073	1.7778467357352172	No Hit
GCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	825	0.2415154877295495	Illumina PCR Primer Index 7 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGC	738	0.216046581750797	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCG	696	0.20375124783001994	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTC	615	0.18003881812566416	No Hit
CCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	613	0.17945332603419858	Illumina PCR Primer Index 7 (95% over 23bp)
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT	417	0.12207510107057229	No Hit
CTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGCT	388	0.11358546574432146	Illumina PCR Primer Index 7 (95% over 24bp)
AAGCAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	372	0.10890152901259686	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11973313270470999	0.0
2	0.0	0.0	0.0	0.694979112569637	0.0
3	0.0	0.0	0.0	0.8984376143539241	0.0
4	0.0	0.0	0.0	1.208455676784946	0.0
5	0.0	0.0	0.0	2.0855228298003765	0.0
6	0.0	0.0	0.0	2.559771423887492	0.0
7	0.0	0.0	0.0	3.038411208660599	0.0
8	0.0	0.0	0.0	3.802478388023174	0.0
9	0.0	0.0	0.0	4.108983497905402	0.0
10	0.0	0.0	0.0	4.928086933865741	0.0
11	0.0	0.0	0.0	5.867508994622255	0.0
12	0.0	0.0	0.0	6.762433656427386	0.0
13	0.0	0.0	0.0	7.07274446490414	0.0
14	0.0	0.0	0.0	7.189842883197255	0.0
15	0.0	0.0	0.0	7.445117435076246	0.0
16	0.0	0.0	0.0	7.899166552007799	0.0
17	0.0	0.0	0.0	8.472948801644062	0.0
18	0.0	0.0	0.0	9.101767307878088	0.0
19	2.927460457327873E-4	0.0	0.0	9.505756850989336	0.0
20	2.927460457327873E-4	0.0	0.0	9.826021025021005	0.0
21	2.927460457327873E-4	0.0	0.0	10.254601235973805	0.0
22	2.927460457327873E-4	0.0	0.0	10.674691811600354	0.0
23	2.927460457327873E-4	0.0	0.0	11.11322538810807	0.0
24	2.927460457327873E-4	0.0	0.0	11.484134628051512	0.0
25	2.927460457327873E-4	0.0	0.0	11.806448024403311	0.0
26	2.927460457327873E-4	0.0	0.0	12.10241427663916	0.0
27	2.927460457327873E-4	0.0	0.0	12.400137005149404	0.0
28	2.927460457327873E-4	0.0	0.0	12.696396003430984	0.0
29	2.927460457327873E-4	0.0	0.0	12.990898525438167	0.0
30	2.927460457327873E-4	0.0	0.0	13.35566009842122	0.0
31	2.927460457327873E-4	0.0	0.0	13.649869874382672	0.0
32	2.927460457327873E-4	0.0	0.0	13.965450111682616	0.0
33	2.927460457327873E-4	0.0	0.0	14.282494079211226	0.0
34	2.927460457327873E-4	0.0	0.0	14.59280488768798	0.0
35	2.927460457327873E-4	0.0	0.0	14.923900665411763	0.0
36	2.927460457327873E-4	0.0	0.0	15.221330647876274	0.0
37	2.927460457327873E-4	0.0	0.0	15.534568916810356	0.0
38	2.927460457327873E-4	0.0	0.0	15.852491122476163	0.0
39	2.927460457327873E-4	0.0	0.0	16.17539001091943	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	25	3.885029E-5	45.000004	21
ATAGCCG	25	3.885029E-5	45.000004	1
TAGCCGT	25	3.885029E-5	45.000004	44
TACGCGG	35	1.208573E-7	45.000004	2
CATCGCC	25	3.885029E-5	45.000004	23
CCAACGG	35	1.208573E-7	45.000004	2
AGTACGG	35	1.208573E-7	45.000004	2
TAGTACG	25	3.885029E-5	45.000004	1
GCGAAGT	20	7.026318E-4	45.0	1
TAGCGAG	20	7.026318E-4	45.0	1
GTAGACG	20	7.026318E-4	45.0	1
TCGCTAA	20	7.026318E-4	45.0	33
GTACTAG	20	7.026318E-4	45.0	1
CTCGCTA	20	7.026318E-4	45.0	32
TATAGCG	20	7.026318E-4	45.0	1
TCATAGG	20	7.026318E-4	45.0	2
ATACGTC	20	7.026318E-4	45.0	41
GACGTAG	30	2.1610067E-6	44.999996	1
CGTTTTT	3425	0.0	43.751823	1
TACGGGA	105	0.0	42.857143	4
>>END_MODULE