FastQCFastQC Report
Sat 18 Jun 2016
SRR3552348_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552348_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249869
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGC71092.845090827593659No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCG63882.5565396267644243No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62282.4925060731823474No Hit
GAATCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTC51102.0450716175275843Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGC23200.9284865269401166Illumina Single End Adapter 1 (95% over 24bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCAAGAACT16460.6587451824756172No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCAAGAACTCGTA12590.5038640247489684No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCT11090.4438325682657712No Hit
GAACTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCT10080.40341138756708517Illumina Single End Adapter 1 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTC8300.3321740592070245No Hit
CTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGCT3890.15568157714642472Illumina Single End Adapter 1 (96% over 25bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATG3760.15047885091788096No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCAAGAACTCGT3430.1372719304915776No Hit
CCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGC3410.1364715110718016Illumina Single End Adapter 1 (95% over 24bp)
GAATGCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTT3180.12726668774437805No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGCAAGAAC3160.1264662683246021No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTAT2810.11245892847852273No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG207.022678E-445.0000041
TCGCAAT253.882015E-545.00000430
TCCGCTC207.022678E-445.00000423
GGATACC207.022678E-445.0000049
TCTACGG302.1586602E-645.0000042
CGAATGG351.2068267E-745.0000042
CCAAGCG207.022678E-445.00000420
CGATGTG253.882015E-545.00000410
GATCTCG253.882015E-545.00000426
CGCAATG253.882015E-545.00000431
GTAGTTG207.022678E-445.0000041
CCCGCAA302.1586602E-645.00000440
GTCGGCT207.022678E-445.00000445
ACGAAGA207.022678E-445.00000412
CGTTTTT32900.044.521281
TACGGCT8300.042.8313267
ACGGCTG8450.042.0710078
CGATGAA2900.041.89655319
GAATGAC1250.041.3999981
ACCACCT2800.040.98214314
Produced by FastQC (version 0.11.5)