FastQCFastQC Report
Sat 18 Jun 2016
SRR3552346_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552346_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences295797
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33851.144365899586541No Hit
GAATCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTC26590.8989273048746268No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGC18900.6389517135062222No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCG18090.6115680686416698No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTT14660.49561016507942945No Hit
GCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC11260.38066647058624664TruSeq Adapter, Index 21 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCT5300.17917693553349087No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGATCGACT4680.1582166147729693No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCT3520.1190005307694128No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGATCGACTCGTA3400.11494369449318283No Hit
CCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC3000.10142090690574955TruSeq Adapter, Index 21 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGCT2990.10108283721606372TruSeq Adapter, Index 14 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCCG302.1600154E-645.00000432
GTGGCGA302.1600154E-645.00000428
AATATTC302.1600154E-645.00000411
ATAACGC302.1600154E-645.00000411
ACCCGCT302.1600154E-645.00000434
AGGGCGC302.1600154E-645.0000046
TCGTTTA207.024782E-445.038
CGTATTA207.024782E-445.012
CGACGGT253.883758E-545.028
GTTTACG207.024782E-445.01
GACGTAT207.024782E-445.010
CGCATCG207.024782E-445.021
ATAGCGG253.883758E-545.02
TAACGCC253.883758E-545.012
CTAACGG351.2078453E-745.02
TCTACGG207.024782E-445.02
TCTAACG253.883758E-545.01
CGGGCTA207.024782E-445.06
TATTACG351.2078453E-745.01
GGACGTA207.024782E-445.09