##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3552346_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 295797 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.323204765430347 28.0 16.0 31.0 16.0 33.0 2 26.59613518730751 30.0 25.0 31.0 16.0 33.0 3 26.940915560333607 30.0 25.0 33.0 16.0 34.0 4 30.74315155326119 33.0 28.0 35.0 19.0 37.0 5 32.275452421762225 35.0 32.0 35.0 28.0 37.0 6 31.00246452803781 35.0 28.0 35.0 17.0 37.0 7 33.08402384067452 35.0 32.0 35.0 28.0 37.0 8 33.82723624647985 35.0 35.0 37.0 31.0 37.0 9 35.21565465505059 37.0 34.0 39.0 30.0 39.0 10 35.20517449467033 37.0 34.0 39.0 30.0 39.0 11 35.52074902720447 37.0 35.0 39.0 30.0 39.0 12 35.27368769798206 37.0 34.0 39.0 30.0 39.0 13 35.43816536340801 37.0 34.0 39.0 30.0 39.0 14 35.79345970378334 38.0 34.0 40.0 27.0 41.0 15 36.18075910168122 38.0 34.0 40.0 30.0 41.0 16 35.86919745636366 38.0 34.0 40.0 30.0 41.0 17 35.92266993918126 37.0 34.0 40.0 30.0 41.0 18 35.55828152415339 38.0 34.0 39.0 27.0 40.0 19 34.98499646717174 37.0 33.0 39.0 27.0 41.0 20 35.587943758726425 37.0 34.0 39.0 30.0 40.0 21 35.44459882960273 37.0 34.0 39.0 29.0 40.0 22 36.21958978623854 38.0 35.0 40.0 31.0 41.0 23 36.74091015121857 38.0 35.0 40.0 32.0 41.0 24 36.82032272132577 38.0 35.0 40.0 32.0 41.0 25 34.68306642731333 37.0 33.0 40.0 25.0 41.0 26 35.545326693644625 37.0 34.0 40.0 30.0 41.0 27 36.12834477699233 38.0 35.0 40.0 31.0 41.0 28 36.26400538206946 38.0 35.0 40.0 31.0 41.0 29 36.30639255976227 38.0 35.0 40.0 31.0 41.0 30 35.517618501877976 38.0 34.0 40.0 29.0 41.0 31 35.63824176715788 38.0 34.0 40.0 30.0 41.0 32 35.602183254055994 38.0 34.0 40.0 29.0 41.0 33 35.717245272940566 38.0 35.0 40.0 29.0 41.0 34 35.638005118375105 38.0 35.0 40.0 28.0 41.0 35 35.588592852530624 38.0 35.0 40.0 27.0 41.0 36 35.37714040372283 38.0 35.0 40.0 26.0 41.0 37 35.4195647690815 38.0 35.0 40.0 26.0 41.0 38 35.0510079547798 38.0 34.0 40.0 25.0 41.0 39 34.97327221033344 38.0 34.0 40.0 24.0 41.0 40 34.697258592886335 38.0 34.0 40.0 23.0 41.0 41 34.50157371440549 38.0 34.0 40.0 23.0 41.0 42 34.820011697211264 38.0 34.0 40.0 24.0 41.0 43 34.902548031251165 38.0 34.0 40.0 24.0 41.0 44 35.0151691869762 38.0 34.0 40.0 24.0 41.0 45 35.00098716349388 38.0 34.0 40.0 25.0 41.0 46 34.81063026332248 38.0 34.0 40.0 24.0 41.0 47 34.7757448520438 37.0 34.0 40.0 24.0 41.0 48 34.73678570100441 37.0 34.0 40.0 24.0 41.0 49 34.72685997491523 37.0 34.0 40.0 24.0 41.0 50 34.59118584705051 37.0 34.0 40.0 24.0 41.0 51 33.380230360686554 36.0 32.0 39.0 20.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 13.0 9 34.0 10 57.0 11 35.0 12 34.0 13 37.0 14 46.0 15 51.0 16 90.0 17 149.0 18 245.0 19 414.0 20 728.0 21 1063.0 22 1449.0 23 2186.0 24 3024.0 25 4233.0 26 5463.0 27 6203.0 28 6387.0 29 7277.0 30 8911.0 31 11810.0 32 16064.0 33 21511.0 34 25713.0 35 33353.0 36 45037.0 37 52957.0 38 35756.0 39 5467.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.70056829514836 18.32709594755863 21.896097661571957 17.076238095721052 2 36.23938038587274 23.149322001237334 23.009023080017716 17.602274532872205 3 31.62371491259208 22.051271649137753 30.032420883240874 16.292592555029294 4 27.037123432624398 23.38833727184522 31.390784896398543 18.18375439913184 5 25.750092123990438 27.20074916243234 27.77715798334669 19.27200073023053 6 24.02965547317924 32.78194166945575 27.662214288853505 15.526188568511511 7 73.81109341879734 5.457120930908697 16.918021480948084 3.813764169345869 8 75.36486171259344 6.409801316443371 13.267883041410155 4.957453929553038 9 69.00948961618948 6.178223579008576 15.573856394757216 9.238430410044726 10 38.908102516252704 23.32106140359774 22.184133037184285 15.586703042965278 11 30.605787076948044 23.94851874765464 26.9465207557886 18.49917341960872 12 26.77444328373851 21.139497696055066 32.87288241598123 19.213176604225197 13 25.949215171215396 21.49447087022519 33.73191749747293 18.824396461086486 14 21.82273653891013 26.201753229410713 31.5993738949347 20.37613633674446 15 20.551256436001715 23.562105092343735 36.33978708370943 19.54685138794511 16 23.469473997369818 22.72707295881973 33.400609201580814 20.402843842229636 17 23.508013941994 22.40590675361819 31.34345513984253 22.74262416454528 18 24.21221310560959 22.556685835218072 31.174758364689296 22.056342694483043 19 24.308224897480365 24.653394050649602 29.884346359158474 21.15403469271156 20 25.956314634698796 23.42856756491783 31.45670848588728 19.15840931449609 21 25.580043070078467 25.00295810978475 30.63181844305385 18.785180377082934 22 22.977244529187246 22.715578589370413 31.785312224261908 22.521864657180434 23 22.851820674313803 24.6199251513707 31.38064280570797 21.147611368607524 24 23.359263278532236 23.299424943457844 31.764689973191075 21.576621804818846 25 22.580688783185764 24.270361092235554 29.90632088898806 23.242629235590627 26 20.83253041782033 24.9350061021579 30.80389591510394 23.42856756491783 27 23.160478300996967 24.092198365771118 30.193342055531325 22.553981277700586 28 20.563765014520094 26.27714277021065 32.162597997951295 20.996494217317956 29 22.417063053377824 25.382272301612254 30.982058641568372 21.21860600344155 30 23.70544664077053 24.594569924644265 31.04967257950554 20.650310855079663 31 25.26563825867064 25.68754923139856 28.31401265056779 20.73279985936301 32 23.56988069520651 27.173703587257474 29.358647991697005 19.897767725839007 33 23.151012349685764 24.58949887929898 28.686903518291263 23.572585252723997 34 23.45392279164427 26.253139822242954 29.041538622771697 21.251398763341076 35 22.73045365571659 27.30149392995872 28.076349658718648 21.89170275560604 36 22.630046957879898 29.671024384966714 26.30148378786803 21.397444869285355 37 23.034378306744152 28.02935797185232 27.95194001291426 20.98432370848927 38 21.244299299857673 28.99691342373317 26.711562321456945 23.047224954952213 39 24.443114703665014 25.249410913565722 27.090538443594763 23.2169359391745 40 23.34675470001386 24.761914421038753 28.98744747242197 22.90388340652542 41 21.411981865941847 26.518862598336018 27.37147435572369 24.697681179998447 42 21.819355842013273 26.879244887541116 28.297109166083494 23.004290104362113 43 22.616524170292465 25.377877395646337 27.270053448817936 24.73554498524326 44 22.756823091512086 24.396123016798683 28.20211158328178 24.644942308407455 45 22.232815072499047 23.80517719922785 27.554708127533413 26.407299600739698 46 22.918082333492226 26.295398533453685 27.631111877402407 23.15540725565168 47 21.522868724158798 25.299107157949543 30.219711491326823 22.95831262656484 48 21.43598481390954 24.761914421038753 29.414091420805484 24.38800934424622 49 21.20575935523349 23.866029743371296 31.74068702522338 23.187523876171834 50 20.640844903768464 23.836955750058316 31.694709547426108 23.827489798747116 51 21.1601199471259 23.35892520884255 29.628765673755986 25.852189170275558 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 80.0 1 257.5 2 435.0 3 3446.5 4 6458.0 5 4442.0 6 2426.0 7 2291.0 8 2156.0 9 2150.0 10 2144.0 11 2024.5 12 1905.0 13 1872.5 14 1840.0 15 1718.5 16 1597.0 17 1519.0 18 1441.0 19 1355.5 20 1270.0 21 1259.5 22 1249.0 23 1194.5 24 1140.0 25 1283.0 26 1725.0 27 2024.0 28 2247.5 29 2471.0 30 2888.0 31 3305.0 32 3844.0 33 4383.0 34 5089.0 35 5795.0 36 6295.0 37 6795.0 38 7431.5 39 8068.0 40 9089.0 41 10110.0 42 11246.0 43 12382.0 44 13866.5 45 15351.0 46 22286.5 47 29222.0 48 26939.5 49 24657.0 50 24575.0 51 24493.0 52 22741.0 53 20989.0 54 19462.0 55 17935.0 56 17409.5 57 16884.0 58 15908.5 59 14933.0 60 14419.0 61 13905.0 62 12925.0 63 11945.0 64 10463.5 65 8982.0 66 7432.0 67 5882.0 68 4832.5 69 3783.0 70 3056.0 71 2329.0 72 1902.5 73 1476.0 74 1237.5 75 820.0 76 641.0 77 465.0 78 289.0 79 192.5 80 96.0 81 72.0 82 48.0 83 46.0 84 44.0 85 25.5 86 7.0 87 5.0 88 3.0 89 2.5 90 2.0 91 1.0 92 0.0 93 0.5 94 1.0 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 295797.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.81069094547371 #Duplication Level Percentage of deduplicated Percentage of total 1 70.84769848531234 25.371050346556263 2 8.622854975446446 6.17580789186706 3 3.7230658063171553 3.999766768790536 4 2.4075355551245123 3.4486204681921357 5 1.9640235624540636 3.516652040233538 6 1.720443223869204 3.6966156347528707 7 1.4311815994543975 3.5876121361437128 8 1.3045152697476716 3.7372474526868116 9 1.2237303912735442 3.944036775823246 >10 6.6731696470444914 34.97095558303533 >50 0.047872088169474955 1.1586030846492463 >100 0.02692804959532966 1.798263418991663 >500 9.973351701973948E-4 0.18929088620964876 >1k 0.005984011021184369 4.405477512067957 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3385 1.144365899586541 No Hit GAATCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTC 2659 0.8989273048746268 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGC 1890 0.6389517135062222 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCG 1809 0.6115680686416698 No Hit GAATGCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTT 1466 0.49561016507942945 No Hit GCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC 1126 0.38066647058624664 TruSeq Adapter, Index 21 (95% over 21bp) GAACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCT 530 0.17917693553349087 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGATCGACT 468 0.1582166147729693 No Hit GAATGACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCT 352 0.1190005307694128 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGATCGACTCGTA 340 0.11494369449318283 No Hit CCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC 300 0.10142090690574955 TruSeq Adapter, Index 21 (95% over 21bp) CTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGCT 299 0.10108283721606372 TruSeq Adapter, Index 14 (95% over 23bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.10852037038915202 0.0 2 0.0 0.0 0.0 0.7424010385500868 0.0 3 0.0 0.0 0.0 0.916844998427976 0.0 4 0.0 0.0 0.0 1.3644492675720172 0.0 5 0.0 0.0 0.0 2.8938765437107206 0.0 6 0.0 0.0 0.0 3.809369263379953 0.0 7 0.0 0.0 0.0 4.249874069040592 0.0 8 0.0 0.0 0.0 4.812760102367502 0.0 9 0.0 0.0 0.0 5.017968404006802 0.0 10 0.0 0.0 0.0 6.0727458358265975 0.0 11 0.0 0.0 0.0 6.8026382958583085 0.0 12 0.0 0.0 0.0 8.05484842645463 0.0 13 0.0 0.0 0.0 8.519694249772648 0.0 14 0.0 0.0 0.0 8.744848663103411 0.0 15 0.0 0.0 0.0 9.06939556520181 0.0 16 0.0 0.0 0.0 9.470008147479522 0.0 17 0.0 0.0 0.0 9.980155309215442 0.0 18 0.0 0.0 0.0 10.518362255195285 0.0 19 0.0 0.0 0.0 10.980841590685504 0.0 20 0.0 0.0 0.0 11.310797607818875 0.0 21 0.0 0.0 0.0 11.645824670297536 0.0 22 0.0 0.0 0.0 12.016349050193206 0.0 23 0.0 0.0 0.0 12.38180238474359 0.0 24 0.0 0.0 0.0 12.676937223839323 0.0 25 0.0 0.0 0.0 12.939279303035528 0.0 26 0.0 0.0 0.0 13.256726741650525 0.0 27 0.0 0.0 0.0 13.528872841847619 0.0 28 0.0 0.0 0.0 13.75673181269587 0.0 29 0.0 0.0 0.0 14.01941196158176 0.0 30 0.0 0.0 0.0 14.312856452229063 0.0 31 0.0 0.0 0.0 14.611371988221652 0.0 32 0.0 0.0 0.0 14.908197175765812 0.0 33 0.0 0.0 0.0 15.232067938484839 0.0 34 0.0 0.0 0.0 15.535992589512402 0.0 35 0.0 0.0 0.0 15.849045122161483 0.0 36 0.0 0.0 0.0 16.143503821877843 0.0 37 0.0 0.0 0.0 16.44370970631886 0.0 38 0.0 0.0 0.0 16.76758046903789 0.0 39 0.0 0.0 0.0 17.14858500931382 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACCCG 30 2.1600154E-6 45.000004 32 GTGGCGA 30 2.1600154E-6 45.000004 28 AATATTC 30 2.1600154E-6 45.000004 11 ATAACGC 30 2.1600154E-6 45.000004 11 ACCCGCT 30 2.1600154E-6 45.000004 34 AGGGCGC 30 2.1600154E-6 45.000004 6 TCGTTTA 20 7.024782E-4 45.0 38 CGTATTA 20 7.024782E-4 45.0 12 CGACGGT 25 3.883758E-5 45.0 28 GTTTACG 20 7.024782E-4 45.0 1 GACGTAT 20 7.024782E-4 45.0 10 CGCATCG 20 7.024782E-4 45.0 21 ATAGCGG 25 3.883758E-5 45.0 2 TAACGCC 25 3.883758E-5 45.0 12 CTAACGG 35 1.2078453E-7 45.0 2 TCTACGG 20 7.024782E-4 45.0 2 TCTAACG 25 3.883758E-5 45.0 1 CGGGCTA 20 7.024782E-4 45.0 6 TATTACG 35 1.2078453E-7 45.0 1 GGACGTA 20 7.024782E-4 45.0 9 >>END_MODULE