FastQCFastQC Report
Sat 18 Jun 2016
SRR3552344_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552344_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences531155
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221624.172416714518361No Hit
GCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGC27690.5213167531134979No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGC26680.5023015880486864No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTC24710.46521260272425186No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCG22750.4283118863608551No Hit
CTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGCT13270.249832911297079No Hit
CCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGC12540.23608927714132408No Hit
TCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGC8160.15362747220679462No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCC7440.14007210701207745No Hit
GAACTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCT6810.12821116246669992No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCATCCTT5970.11239656973952988No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCATCCTTCGTA5960.11220830077849217No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCT5470.10298312168764297No Hit
ACTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGC5460.10279485272660523No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT207.0298545E-445.00000428
CGGTTCA207.0298545E-445.00000441
ACGAGAC207.0298545E-445.00000439
ATACCGG207.0298545E-445.0000042
CGTATTT253.8879632E-545.01
ACGTTAG253.8879632E-545.01
TTAGTCA253.8879632E-545.032
GATAGCG253.8879632E-545.01
CATACGC302.1632895E-644.99999620
GGCATAC302.1632895E-644.99999618
CGCGTAT302.1632895E-644.99999624
ACGCGTA302.1632895E-644.99999623
CGTTTTT105400.044.3382341
TCGTTCA501.0786607E-940.516
ACCACCG1000.040.514
TAGGGCG1300.039.8076935
TGCGGGT850.039.7058834
GTTTTTT119750.039.7014582
GGCCGAT403.4535333E-739.3750048
TGCGAAG403.4535333E-739.3750041
Produced by FastQC (version 0.11.5)